CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown ; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="AgRP/ART was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human AgRP/ART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF704) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown ; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF704-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89203-25ul | |||
704-AG) was serially diluted and captured by Mouse Anti-Human AgRP/ART Monoclonal Antibody (Catalog # MAB8312) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human AgRP/ART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF704) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human AgRP/ART (Catalog # 704-AG) was serially diluted and captured by Mouse Anti-Human AgRP/ART Monoclonal Antibody (Catalog # MAB8312) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human AgRP/ART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF704) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8312-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8311-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-12026-0.025mg | |||
704-AG) was serially diluted and captured by Mouse Anti-Human AgRP/ART Monoclonal Antibody (Catalog # 
704-AG) was serially diluted and captured by Mouse Anti-Human AgRP/ART Monoclonal Antibody (Catalog # ![N/A AgRP/ART [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_AgRP_DAGR00_ELISA_569.jpg "N/A AgRP/ART [HRP]")
![N/A AgRP/ART [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_AgRP_DAGR00_ELISA_570.jpg)
![N/A AgRP/ART [HRP]](http://images.novusbio.com/fullsize/elisa/AgRP_DAGR00_ELISA_27.jpg)
![ELISA: Mouse AgRP/ART ELISA Kit (Colorimetric) [NBP2-78744] - Samples were spiked with high concentrations of Mouse AgRP/ART and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-78744_mouse-agrp-art-elisa-kit-colorimetric-132202416235728.jpg "ELISA: Mouse AgRP/ART ELISA Kit (Colorimetric) [NBP2-78744] - Samples were spiked with high concentrations of Mouse AgRP/ART and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse AgRP/ART ELISA Kit (Colorimetric) [NBP2-78744] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-78744_mouse-agrp-art-elisa-kit-colorimetric-432024919287.jpg)
![Immunohistochemistry: AgRP/ART Antibody [NB100-1540] - Staining of PFA-perfused cryosection of Human Hypothalamus. Antibody at 0.05 ug/mL. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.](http://images.novusbio.com/fullsize/AgRP-ART-Antibody-Immunohistochemistry-NB100-1540-img0001.jpg "Immunohistochemistry: AgRP/ART Antibody [NB100-1540] - Staining of PFA-perfused cryosection of Human Hypothalamus. Antibody at 0.05 ug/mL. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.")
![ELISA: Mouse AgRP/ART ELISA Kit (Chemiluminescence) [NBP2-66664] - Samples were spiked with high concentrations of Mouse AgRP/ART and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66664_mouse-agrp-art-elisa-kit-chemiluminescence-13220241685330.jpg "ELISA: Mouse AgRP/ART ELISA Kit (Chemiluminescence) [NBP2-66664] - Samples were spiked with high concentrations of Mouse AgRP/ART and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse AgRP/ART ELISA Kit (Chemiluminescence) [NBP2-66664] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66664_mouse-agrp-art-elisa-kit-chemiluminescence-432024963983.jpg)
![Immunohistochemistry-Paraffin: AgRP/ART Antibody (MM0085-32B12) [NBP2-12026] - 10% Buffer formalin fixed and parffine embedded human placetal tissue section (4um) is subjected IHC staining using NBP2-12026. Tissue section was pretreated in citric buffer (pH 6.0) with microwave for antige retrieval before IHC is applied.](http://images.novusbio.com/fullsize/AgRP-ART-Antibody-MM0085-32B12-Immunohistochemistry-Paraffin-NBP2-12026-img0001.jpg "<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: AgRP/ART Antibody (MM0085-32B12) [NBP2-12026] - 10% Buffer formalin fixed and parffine embedded human placetal tissue section (4um) is subjected IHC staining using NBP2-12026. Tissue section was pretreated in citric buffer (pH 6.0) with microwave for antige retrieval before IHC is applied.")
