Aconitase 1 Antibody (SR2310) - BSA Free

Western Blot: Aconitase 1 Antibody (SR2310) [NBP3-22405] - Western blot analysis using NBP3-22405 in (1) mouse kidney lysate; (2) HepG2 cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: SR2310
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Aconitase 1 Antibody (SR2310) - BSA Free Summary

• Description: Novus Biologicals Rabbit Aconitase 1 Antibody (SR2310) - BSA Free (NBP3-22405) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human Aconitase 1 (Uniprot #: P21399)
• Specificity: Detects endogenous levels of total Aconitase 1
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACO1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 98 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Aconitase 1 Antibody (SR2310) - BSA Free

• aconitase 1, soluble
• Aconitase
• aconitate hydratase
• Citrate hydro-lyase
• cytoplasmic aconitate hydratase
• EC 4.2.1
• EC 4.2.1.3
• Ferritin repressor protein
• IREB1IREBP1
• IRE-BP 1
• IREBP
• Iron regulatory protein 1
• iron-responsive element binding protein 1
• Iron-responsive element-binding protein 1
• IRP1ACONS

Background

Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACO1