Genetic Strategies: Knockdown Validated: Fumarase Antibody [NBP1-31336] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ...read more
Western Blot: Fumarase Antibody [NBP1-31336] - Representative western blots showing protein expression of fumarase and key enzymes in OXPHOS. VDAC was used as a loading control (n = 3). Image collected and cropped by ...read more
Immunocytochemistry/ Immunofluorescence: Fumarase Antibody [NBP1-31336] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Fumarate hydratase protein stained by Fumarate hydratase antibody diluted at 1:500.Blue: ...read more
Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-31336] - Analysis of paraffin-embedded NCIN87 xenograft, using fumarate hydratase antibody at 1:500 dilution.
Western Blot: Fumarase Antibody [NBP1-31336] - A. 50 ug mouse liver lysate/extract 7.5% SDS-PAGE Fumarate hydratase antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect ...read more
Western Blot: Fumarase Antibody [NBP1-31336] - A.50 ug rat liver lysate/extract 10% SDS-PAGE Fumarate hydratase antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the ...read more
Western Blot: Fumarase Antibody [NBP1-31336] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody diluted at 1:1000. The HRP-conjugated ...read more
55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP1-31336 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS, 20% Glycerol (pH7).
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for Fumarase Antibody
fumarate hydratase, mitochondrial
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for Fumarase Antibody (NBP1-31336)(1)
We have publications tested in 1 confirmed species: Human.
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