Wnt-2 Antibody

Western blot shows lysates of human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Wnt-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3464) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Wnt-2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Wnt‑2 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using 10 µg/mL Goat Anti-Human Wnt‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3464) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Wnt-2 Antibody Summary

• Immunogen: E. coli-derived recombinant human Wnt‑2
  Thr33-Glu75, Leu222-Arg292
  Accession # P09544
• Specificity: Detects human Wnt-2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Wnt-7a and rhWnt-7b is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: WNT2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-2 Antibody

• INT1L1
• INT1L1secreted growth factor
• Int-1-like protein 1
• Int-1-related protein
• IRP
• IRPprotein Wnt-2
• wingless-type MMTV integration site family member 2
• Wnt2
• Wnt-2

Background

Human Wnt-2 is a 35 kDa, secreted, glycosylated member of the Wnt family of developmental proteins. It is considered a class 1 Wnt based on its strong transforming activity on C57MG cells. Human Wnt-2 is synthesized as a 360 amino acid (aa) precursor that contains a 335 aa mature region. The mature region contains 24 cysteines and two potential N-linked glycosylation sites. Mature human Wnt-2 is 96% aa identical to mature mouse and rat Wnt-2. Wnt-2 is produced by mammary myoepithelial cells, endometrial epithelial cells, and breast fibroblasts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Wnt-2 Antibody (AF3464)(1)

Publication using AF3464

• Emily Coffey, Donna R. Newman, Philip L. Sannes Expression of Fibroblast Growth Factor 9 in Normal Human Lung and Idiopathic Pulmonary Fibrosis Journal of Histochemistry & Cytochemistry 2013-09-01 [PMID: 23797050]

Bioinformatics

• Gene Symbol: WNT2
• Uniprot:
  • P09544()