Western Blot: Aconitase 2 Antibody [NBP1-32781] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [C1C3] diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: Aconitase 2 Antibody [NBP1-32781] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aconitase 2 protein stained by Aconitase 2 antibody [C1C3] diluted at ...read more
Western Blot: Aconitase 2 Antibody [NBP1-32781] - Sample (20 ug of whole cell lysate) A: mouse brain 7. 5% SDS PAGE, antibody diluted at 1:20000.
Western Blot: Aconitase 2 Antibody [NBP1-32781] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE, antibody diluted at 1:10000.
Immunoprecipitation: Aconitase 2 Antibody [NBP1-32781] - HeLa whole cell extracts using 5 ug of Aconitase 2 antibody [C1C3]. Western blot analysis was performed using Aconitase 2 antibody [C1C3]. EasyBlot anti-Rabbit ...read more
85 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NBP1-32781 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Immunogen affinity purified
Alternate Names for Aconitase 2 Antibody
aconitase 2, mitochondrial
aconitate hydratase, mitochondrial
The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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