Biological Strategies: Western Blot: IRP2 Antibody [NB100-1798] - Raw macrophages (mouse) were either left untreated (lane 1) or treated overnight with 100mM DFO (lane 2) or 100mM hemin (lane 3). Proteins ...read more
Immunohistochemistry-Paraffin: IRP2 Antibody [NB100-1798] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human placenta using 1:300 conc. of IRP2 antibody on a Bond Rx autostainer (Leica Biosystems). The ...read more
Western Blot: IRP2 Antibody [NB100-1798] - Detection of IRP2 in mouse liver lysate.
Western Blot: IRP2 Antibody [NB100-1798] - Detection of IRP2 in RAW 264.7 lysate using NB100-1798. Lane 1: Lot B Lane 2: Lot C
This IRP2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. Use in Gel super shift assay reported in scientific literature (PMID 26752519).
Preliminary results have shown poor reactivity against human protein. Immunogen has 94% homology with human, primate and rat proteins. Human reactivity reported in scientific literature (PMID: 28888202).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for IRP2 Antibody
Iron regulatory protein 2
iron-responsive element binding protein 2
iron-responsive element-binding protein 2
Iron regulatory proteins -1 and -2 (IRP1, IRP2) are homologous cytoplasmic RNA-binding proteins that controls cellular iron metabolism and maintain systemic iron homeostasis in vertebrates along with serving some additional iron-independent functions. IRP2 belongs to aconitase/IPM isomerase family and it binds to IRES (iron-responsive elements), which are stem-loop structures found in 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. IRP2-IRE binding results in the repression of its mRNA translation and its binding to transferrin receptor mRNA inhibits the degradation of this otherwise quickly degradable mRNA. IRP2 is a substrate of FBXL5, an F-box protein that assembles together with Skp1, Cul1 and Rbx1 into an E3 ubiquitin ligase complex. When iron/O2 are high, IRP2 gets ubiquitinated/degraded by SCF complex containing FBXL5, whereas under conditions of low iron/O2, FBXL5 is degraded preventing IRP2 ubiquitination leading to its RNA-binding activity. IRP2 deficient mice depicts microcytosis, misregulated iron metabolism (in CNS, intestine as well as liver associated with FTH1 and FTL up-regulation) and iron deficiency in splenic macrophages associated with down-regulation of FTH1, FTL, and FPN1.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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