IRP2 Antibody


Western Blot: IRP2 Antibody [NB100-1798] - Detection of IRP2 in RAW 264.7 lysate using NB100-1798. Lane 1: Lot B Lane 2: Lot C
Western Blot: IRP2 Antibody [NB100-1798] - Detection of IRP2 in mouse liver lysate.
Western Blot: IRP2 Antibody [NB100-1798] - Raw macrophages (mouse) were either left untreated (lane 1) or treated overnight with 100mM DFO (lane 2) or 100mM hemin (lane 3). Proteins extracts were subjected to western more

Product Details

Reactivity MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, GS
1.15 mg/ml

IRP2 Antibody Summary

A synthetic peptide made to an internal portion of the murine IRP2 protein sequence (between residues 100-200). [UniProt Q811J3]
Predicted Species
Human (94%), Rat (94%), Primate (94%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
1.15 mg/ml
Immunogen affinity purified


Application Notes
This IRP2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. Use in Gel super shift assay reported in scientific literature (PMID 26752519).
Read Publications using
NB100-1798 in the following applications:

  • GS
    1 publication
  • WB
    4 publications

Reactivity Notes

Preliminary results have shown poor reactivity against human protein. Immunogen has 94% homology with human, primate and rat proteins.

Alternate Names for IRP2 Antibody

  • ACO3
  • EC
  • FLJ23381
  • IRE-BP 2
  • Iron regulatory protein 2
  • iron-responsive element binding protein 2
  • iron-responsive element-binding protein 2


Iron regulatory proteins -1 and -2 (IRP1, IRP2) are homologous cytoplasmic RNA-binding proteins that controls cellular iron metabolism and maintain systemic iron homeostasis in vertebrates along with serving some additional iron-independent functions. IRP2 belongs to aconitase/IPM isomerase family and it binds to IRES (iron-responsive elements), which are stem-loop structures found in 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. IRP2-IRE binding results in the repression of its mRNA translation and its binding to transferrin receptor mRNA inhibits the degradation of this otherwise quickly degradable mRNA. IRP2 is a substrate of FBXL5, an F-box protein that assembles together with Skp1, Cul1 and Rbx1 into an E3 ubiquitin ligase complex. When iron/O2 are high, IRP2 gets ubiquitinated/degraded by SCF complex containing FBXL5, whereas under conditions of low iron/O2, FBXL5 is degraded preventing IRP2 ubiquitination leading to its RNA-binding activity. IRP2 deficient mice depicts microcytosis, misregulated iron metabolism (in CNS, intestine as well as liver associated with FTH1 and FTL up-regulation) and iron deficiency in splenic macrophages associated with down-regulation of FTH1, FTL, and FPN1.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRP2 Antibody (NB100-1798)(5)

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Product General Protocols

View specific protocols for IRP2 Antibody (NB100-1798): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IRP2 Antibody (NB100-1798) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-1798AF405
Alexa Fluor (R) 488 Labeled NB100-1798AF488
Alexa Fluor (R) 647 Labeled NB100-1798AF647
Alexa Fluor (R) 700 Labeled NB100-1798AF700
Biotin Labeled NB100-1798B
DyLight 405 Labeled NB100-1798V
DyLight 488 Labeled NB100-1798G
DyLight 550 Labeled NB100-1798R
DyLight 650 Labeled NB100-1798C
FITC Labeled NB100-1798F
HRP Labeled NB100-1798H

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Blogs on IRP2

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Contact Information

Product PDFs


Gene Symbol IREB2

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