IRP2 Antibody

Images

 
Biological Strategies: Western Blot: IRP2 Antibody [NB100-1798] - Raw macrophages (mouse) were either left untreated (lane 1) or treated overnight with 100mM DFO (lane 2) or 100mM hemin (lane 3). Proteins ...read more
Immunohistochemistry-Paraffin: IRP2 Antibody [NB100-1798] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human placenta using 1:300 conc. of IRP2 antibody on a Bond Rx autostainer (Leica Biosystems). The ...read more
Western Blot: IRP2 Antibody [NB100-1798] - Detection of IRP2 in mouse liver lysate.
Western Blot: IRP2 Antibody [NB100-1798] - Detection of IRP2 in RAW 264.7 lysate using NB100-1798. Lane 1: Lot B Lane 2: Lot C

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, GS, ICC/IF, IHC, GS
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.15 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

IRP2 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the murine IRP2 protein sequence (between residues 100-200). [UniProt Q811J3]
Localization
Cytoplasmic
Predicted Species
Rat (94%), Primate (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IREB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Gel Super Shift Assays reported in scientific literature (PMID 26752519)
  • Gel Supershift Assay
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 1:500-1:1000
Control
NIH 3T3 Whole Cell Lysate
Publications
Read Publications using
NB100-1798 in the following applications:

  • GS
    1 publication
  • IHC
    1 publication
  • WB
    7 publications

Reactivity Notes

Preliminary results have shown poor reactivity against human protein. Immunogen displays the following percentage of sequence identity for non-tested species: primate (94%) and rat (94%) proteins. Human reactivity reported in scientific literature (PMID: 28888202).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.15 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IRP2 Antibody

  • ACO3
  • EC 4.2.1.3
  • FLJ23381
  • IRE-BP 2
  • Iron regulatory protein 2
  • iron-responsive element binding protein 2
  • iron-responsive element-binding protein 2
  • IRP2IRP2AD

Background

Iron regulatory proteins -1 and -2 (IRP1, IRP2) are homologous cytoplasmic RNA-binding proteins that controls cellular iron metabolism and maintain systemic iron homeostasis in vertebrates along with serving some additional iron-independent functions. IRP2 belongs to aconitase/IPM isomerase family and it binds to IRES (iron-responsive elements), which are stem-loop structures found in 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. IRP2-IRE binding results in the repression of its mRNA translation and its binding to transferrin receptor mRNA inhibits the degradation of this otherwise quickly degradable mRNA. IRP2 is a substrate of FBXL5, an F-box protein that assembles together with Skp1, Cul1 and Rbx1 into an E3 ubiquitin ligase complex. When iron/O2 are high, IRP2 gets ubiquitinated/degraded by SCF complex containing FBXL5, whereas under conditions of low iron/O2, FBXL5 is degraded preventing IRP2 ubiquitination leading to its RNA-binding activity. IRP2 deficient mice depicts microcytosis, misregulated iron metabolism (in CNS, intestine as well as liver associated with FTH1 and FTL up-regulation) and iron deficiency in splenic macrophages associated with down-regulation of FTH1, FTL, and FPN1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRP2 Antibody (NB100-1798)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: GS, IHC, WB.


Filter By Application
GS
(1)
IHC
(1)
WB
(7)
All Applications
Filter By Species
Human
(3)
Mouse
(5)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB100-1798 Applications Species
Ito F, Kato K, Yanatori I Et al. Ferroptosis-dependent extracellular vesicles from macrophage contribute to asbestos-induced mesothelial carcinogenesis through loading ferritin Redox biology 2021-10-21 [PMID: 34700146] (Human) Human
Cheng Z, Akatsuka S, Li GH et al. Ferroptosis resistance determines high susceptibility of murine A/J strain to iron-induced renal carcinogenesis Cancer Science 2022-01-01 [PMID: 34699654] (WB, IHC) WB, IHC
Jiang Z, Wang Z, Chen L et al. Artesunate induces ER-derived-ROS-mediated cell death by disrupting labile iron pool and iron redistribution in hepatocellular carcinoma cells American journal of cancer research 2021-03-01 [PMID: 33791148] (WB, Human) WB Human
Masaldan S, Clatworthy SAS, Gamell C et al. Iron accumulation in senescent cells is coupled with impaired ferritinophagy and inhibition of ferroptosis Redox Biol 2017-09-01 [PMID: 28888202] (WB, Human) WB Human
Cloonan SM, Glass K, Laucho-Contreras ME et al. Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice. Nat Med 2016-02-01 [PMID: 26752519] (WB, GS, Mouse) WB, GS Mouse
Whitnall M, Rahmanto YS, Huang ML et al. Identification of nonferritin mitochondrial iron deposits in a mouse model of Friedreich ataxia Proc Natl Acad Sci U S A 2012-11-20 [PMID: 23169664] (WB, Mouse) WB Mouse
Yoshihara D, Fujiwara N, Kato S, Sakiyama H, Eguchi H, Suzuki K. Alterations in renal iron metabolism caused by a copper/zinc-superoxide dismutase deficiency. Free Radic Res. 2012-04-10 [PMID: 22435664] (WB, Mouse) WB Mouse
Masaratana P, Patel N, Latunde-Dada GO, Vaulont S, Simpson RJ, McKie AT. Regulation of iron metabolism in Hamp (-/-) mice in response to iron-deficient diet. Eur J Nutr. 2012-01-13 [PMID: 22241739] (WB, Mouse) WB Mouse
Regan RF, Chen M, Li Z et al. Neurons lacking iron regulatory protein-2 are highly resistant to the toxicity of hemoglobin. Neurobiol Dis 2008-08-01 [PMID: 18571425] (Mouse) Mouse

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Product General Protocols

View specific protocols for IRP2 Antibody (NB100-1798): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IREB2
Entrez
Uniprot