IRP2 Antibody


Biological Strategies: Western Blot: IRP2 Antibody [NB100-1798] - Raw macrophages (mouse) were either left untreated (lane 1) or treated overnight with 100mM DFO (lane 2) or 100mM hemin (lane 3). Proteins more
Immunohistochemistry-Paraffin: IRP2 Antibody [NB100-1798] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human placenta using 1:300 conc. of IRP2 antibody on a Bond Rx autostainer (Leica Biosystems). The more
Western Blot: IRP2 Antibody [NB100-1798] - Detection of IRP2 in mouse liver lysate.
Western Blot: IRP2 Antibody [NB100-1798] - Detection of IRP2 in RAW 264.7 lysate using NB100-1798. Lane 1: Lot B Lane 2: Lot C

Product Details

Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, GS
1.15 mg/ml
Validated by:

Biological Strategies


IRP2 Antibody Summary

A synthetic peptide made to an internal portion of the murine IRP2 protein sequence (between residues 100-200). [UniProt Q811J3]
Predicted Species
Rat (94%), Primate (94%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Gel Supershift Assay
Application Notes
This IRP2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. Use in Gel super shift assay reported in scientific literature (PMID 26752519).
NIH 3T3 Whole Cell Lysate
Read Publications using
NB100-1798 in the following applications:

  • GS
    1 publication
  • WB
    5 publications

Reactivity Notes

Preliminary results have shown poor reactivity against human protein. Immunogen displays the following percentage of sequence identity for non-tested species: human (94%), primate (94%) and rat (94%) proteins. Human reactivity reported in scientific literature (PMID: 28888202).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
1.15 mg/ml
Immunogen affinity purified

Alternate Names for IRP2 Antibody

  • ACO3
  • EC
  • FLJ23381
  • IRE-BP 2
  • Iron regulatory protein 2
  • iron-responsive element binding protein 2
  • iron-responsive element-binding protein 2


Iron regulatory proteins -1 and -2 (IRP1, IRP2) are homologous cytoplasmic RNA-binding proteins that controls cellular iron metabolism and maintain systemic iron homeostasis in vertebrates along with serving some additional iron-independent functions. IRP2 belongs to aconitase/IPM isomerase family and it binds to IRES (iron-responsive elements), which are stem-loop structures found in 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. IRP2-IRE binding results in the repression of its mRNA translation and its binding to transferrin receptor mRNA inhibits the degradation of this otherwise quickly degradable mRNA. IRP2 is a substrate of FBXL5, an F-box protein that assembles together with Skp1, Cul1 and Rbx1 into an E3 ubiquitin ligase complex. When iron/O2 are high, IRP2 gets ubiquitinated/degraded by SCF complex containing FBXL5, whereas under conditions of low iron/O2, FBXL5 is degraded preventing IRP2 ubiquitination leading to its RNA-binding activity. IRP2 deficient mice depicts microcytosis, misregulated iron metabolism (in CNS, intestine as well as liver associated with FTH1 and FTL up-regulation) and iron deficiency in splenic macrophages associated with down-regulation of FTH1, FTL, and FPN1.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRP2 Antibody (NB100-1798)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: GS, WB.

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Showing Publications 1 - 6 of 6.
Publications using NB100-1798 Applications Species
Masaldan S, Clatworthy SAS, Gamell C et al. Iron accumulation in senescent cells is coupled with impaired ferritinophagy and inhibition of ferroptosis Redox Biol 2017 Sep 01 [PMID: 28888202] (WB, Human) WB Human
Cloonan SM, Glass K, Laucho-Contreras ME et al. Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice. Nat. Med. 2016 Feb 01 [PMID: 26752519] (WB, GS, Mouse)

IRP2 antibody was used at 2.5ug/ml concentration for gel supershift assay/electrophoretic mobility shift assay (EMSA) and in Western blot assay in experiments involving cigarette smoke-induced murine bronchitis and emphysema.
WB, GS Mouse
Whitnall M, Rahmanto YS, Huang ML et al. Identification of nonferritin mitochondrial iron deposits in a mouse model of Friedreich ataxia Proc Natl Acad Sci U S A 2012 Nov 20 [PMID: 23169664] (WB, Mouse) WB Mouse
Yoshihara D, Fujiwara N, Kato S, Sakiyama H, Eguchi H, Suzuki K. Alterations in renal iron metabolism caused by a copper/zinc-superoxide dismutase deficiency. Free Radic Res. 2012 Apr 10. [PMID: 22435664] (WB, Mouse) WB Mouse
Masaratana P, Patel N, Latunde-Dada GO, Vaulont S, Simpson RJ, McKie AT. Regulation of iron metabolism in Hamp (-/-) mice in response to iron-deficient diet. Eur J Nutr. 2012 Jan 13. [PMID: 22241739] (WB, Mouse) WB Mouse
Regan RF, Chen M, Li Z et al. Neurons lacking iron regulatory protein-2 are highly resistant to the toxicity of hemoglobin. Neurobiol Dis 2008 Aug [PMID: 18571425] (Mouse) Mouse

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Product General Protocols

View specific protocols for IRP2 Antibody (NB100-1798): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-1798AF350
Alexa Fluor 405 NB100-1798AF405
Alexa Fluor 488 NB100-1798AF488
Alexa Fluor 532 NB100-1798AF532
Alexa Fluor 594 NB100-1798AF594
Alexa Fluor 647 NB100-1798AF647
Alexa Fluor 700 NB100-1798AF700
Alexa Fluor 750 NB100-1798AF750
Biotin NB100-1798B
DyLight 350 NB100-1798UV
DyLight 405 NB100-1798V
DyLight 488 NB100-1798G
DyLight 550 NB100-1798R
DyLight 594 NB100-1798DL594
DyLight 650 NB100-1798C
DyLight 680 NB100-1798FR
DyLight 755 NB100-1798IR
FITC NB100-1798F
HRP NB100-1798H
Janelia Fluor 549 NB100-1798JF549
Janelia Fluor 646 NB100-1798JF646

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Gene Symbol IREB2