IRP2 Antibody

Images

 
Western Blot: IRP2 Antibody [NB100-1798] - Raw macrophages (mouse) were either left untreated (lane 1) or treated overnight with 100mM DFO (lane 2) or 100mM hemin (lane 3). Proteins extracts were subjected to western ...read more
Western Blot: IRP2 Antibody [NB100-1798] - Detection of IRP2 in mouse liver lysate.
Western Blot: IRP2 Antibody [NB100-1798] - Detection of IRP2 in RAW 264.7 lysate using NB100-1798. Lane 1: Lot B Lane 2: Lot C

Product Details

Summary
Reactivity Mu, Hu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, GS
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.15 mg/ml
   

Biological Strategies

     

IRP2 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the murine IRP2 protein sequence (between residues 100-200). [UniProt Q811J3]
Localization
Cytoplasmic
Predicted Species
Human (94%), Rat (94%), Primate (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IREB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Gel Supershift Assay
Application Notes
This IRP2 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. Use in Gel super shift assay reported in scientific literature (PMID 26752519).
Positive Control
NIH 3T3 Lysate (NB800-PC8)
Publications
Read Publications using
NB100-1798 in the following applications:

  • GS
    1 publication
  • WB
    5 publications

Reactivity Notes

Preliminary results have shown poor reactivity against human protein. Immunogen has 94% homology with human, primate and rat proteins.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.15 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IRP2 Antibody

  • ACO3
  • EC 4.2.1.3
  • FLJ23381
  • IRE-BP 2
  • Iron regulatory protein 2
  • iron-responsive element binding protein 2
  • iron-responsive element-binding protein 2
  • IRP2IRP2AD

Background

Iron regulatory proteins -1 and -2 (IRP1, IRP2) are homologous cytoplasmic RNA-binding proteins that controls cellular iron metabolism and maintain systemic iron homeostasis in vertebrates along with serving some additional iron-independent functions. IRP2 belongs to aconitase/IPM isomerase family and it binds to IRES (iron-responsive elements), which are stem-loop structures found in 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. IRP2-IRE binding results in the repression of its mRNA translation and its binding to transferrin receptor mRNA inhibits the degradation of this otherwise quickly degradable mRNA. IRP2 is a substrate of FBXL5, an F-box protein that assembles together with Skp1, Cul1 and Rbx1 into an E3 ubiquitin ligase complex. When iron/O2 are high, IRP2 gets ubiquitinated/degraded by SCF complex containing FBXL5, whereas under conditions of low iron/O2, FBXL5 is degraded preventing IRP2 ubiquitination leading to its RNA-binding activity. IRP2 deficient mice depicts microcytosis, misregulated iron metabolism (in CNS, intestine as well as liver associated with FTH1 and FTL up-regulation) and iron deficiency in splenic macrophages associated with down-regulation of FTH1, FTL, and FPN1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRP2 Antibody (NB100-1798)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: GS, WB.


Filter By Application
GS
(1)
WB
(5)
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(1)
Mouse
(5)
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Showing Publications 1 - 6 of 6.
Publications using NB100-1798 Applications Species
Masaldan S, Clatworthy SAS, Gamell C et al. Iron accumulation in senescent cells is coupled with impaired ferritinophagy and inhibition of ferroptosis Redox Biol 2017 Sep 01 [PMID: 28888202] (WB, Human) WB Human
Cloonan SM, Glass K, Laucho-Contreras ME et al. Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice. Nat. Med. 2016 Feb 01 [PMID: 26752519] (WB, GS, Mouse)

Details:
IRP2 antibody was used at 2.5ug/ml concentration for gel supershift assay/electrophoretic mobility shift assay (EMSA) and in Western blot assay in experiments involving cigarette smoke-induced murine bronchitis and emphysema.
WB, GS Mouse
Whitnall M, Rahmanto YS, Huang ML et al. Identification of nonferritin mitochondrial iron deposits in a mouse model of Friedreich ataxia Proc Natl Acad Sci U S A 2012 Nov 20 [PMID: 23169664] (WB, Mouse) WB Mouse
Yoshihara D, Fujiwara N, Kato S, Sakiyama H, Eguchi H, Suzuki K. Alterations in renal iron metabolism caused by a copper/zinc-superoxide dismutase deficiency. Free Radic Res. 2012 Apr 10. [PMID: 22435664] (WB, Mouse) WB Mouse
Masaratana P, Patel N, Latunde-Dada GO, Vaulont S, Simpson RJ, McKie AT. Regulation of iron metabolism in Hamp (-/-) mice in response to iron-deficient diet. Eur J Nutr. 2012 Jan 13. [PMID: 22241739] (WB, Mouse) WB Mouse
Regan RF, Chen M, Li Z et al. Neurons lacking iron regulatory protein-2 are highly resistant to the toxicity of hemoglobin. Neurobiol Dis 2008 Aug [PMID: 18571425] (Mouse) Mouse

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Product General Protocols

View specific protocols for IRP2 Antibody (NB100-1798): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IRP2 Antibody (NB100-1798) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-1798AF405
Alexa Fluor 488 Labeled NB100-1798AF488
Alexa Fluor 647 Labeled NB100-1798AF647
Alexa Fluor 700 Labeled NB100-1798AF700
Biotin Labeled NB100-1798B
DyLight 405 Labeled NB100-1798V
DyLight 488 Labeled NB100-1798G
DyLight 550 Labeled NB100-1798R
DyLight 650 Labeled NB100-1798C
FITC Labeled NB100-1798F
HRP Labeled NB100-1798H

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Bioinformatics

Gene Symbol IREB2
Entrez
Uniprot