Aconitase 1 Antibody (22C10) - BSA Free

Western Blot: Aconitase 1 Antibody (22C10) [NBP3-26529] - Positive Western Blot detected in: Hela whole cell lysate. All lanes: Aconitase 1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 100 kDa Observed band size: 95-130 kDa

Immunocytochemistry/Immunofluorescence: Aconitase 1 Antibody (22C10) [NBP3-26529] - Staining of HepG2 cell with Aconitase 1 Antibody (22C10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 511-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: Aconitase 1 Antibody (22C10) [NBP3-26529] - Overlay Peak curve showing HepG2 cells stained with Aconitase 1 Antibody (22C10) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 22C10
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Aconitase 1 Antibody (22C10) - BSA Free Summary

• Description: Novus Biologicals Rabbit Aconitase 1 Antibody (22C10) - BSA Free (NBP3-26529) is a recombinant monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human Aconitase 1 [UniProt P21399]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACO1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Aconitase 1 Antibody (22C10) - BSA Free

• aconitase 1, soluble
• Aconitase
• aconitate hydratase
• Citrate hydro-lyase
• cytoplasmic aconitate hydratase
• EC 4.2.1
• EC 4.2.1.3
• Ferritin repressor protein
• IREB1IREBP1
• IRE-BP 1
• IREBP
• Iron regulatory protein 1
• iron-responsive element binding protein 1
• Iron-responsive element-binding protein 1
• IRP1ACONS

Background

Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACO1