Serpin A5/Protein C Inhibitor Antibody (210326) [Unconjugated]

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Serpin A5 Monoclonal Antibody (Catalog # MAB1266, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

• Reactivity: Hu
• Applications: WB, IP, CyTOF-ready, Flow
• Clone: 210326
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Serpin A5/Protein C Inhibitor Antibody (210326) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Serpin A5/Protein C
  His20-Pro406
  Accession # P05154
• Specificity: Detects human Serpin A5/Protein C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Serpin A1, A3, F2, C1, or recombinant mouse Serpin A5 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SERPINA5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunoprecipitation 25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A5/Protein C Inhibitor Antibody (210326) [Unconjugated]

• Acrosomal serine protease inhibitor
• antitrypsin), member 5
• member 5
• PAI-3
• PAI3PLANH3
• PCI
• PCIplasminogen activator inhibitor III
• Plasminogen activator inhibitor 3
• plasminogen activator inhibitor-3
• PROCIplasma serine protease inhibitor
• Protein C inhibitor
• serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
• Serpin A5
• serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
• SERPNA5

Background

Serpin A5 is the a member of the Serpin superfamily and inhibits a variety of serine proteases such as protein C, plasminogen activators, thrombin, factor Xa, several kallekreins and acrosin (1). Serpin A5 is synthesized in the liver and secreted in plasma. It is found in numerous steroid-responsive organs and has been detected in saliva, cerebral spinal fluid, amniotic fluid, tears and semen. Because of its protease targets and regulated expression patterns, Serpin A5 has been proposed to play a role in processes such as blood coagulation, fertilization and carcinogenesis (2, 3). Similar to Serpins C1 and D1, its thrombin inhibitory activity is enhanced by heparin.

1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
2. Palmier, D. et al. (2002) J. Biol. Chem. 277:40950.
3. Geiger, M. et al. (1996) Immunopharmacology 32:53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin A5/Protein C Inhibitor Antibody (MAB1266)(2)

Publications using MAB1266

• AM Crist, KM Hinkle, X Wang, CM Moloney, BJ Matchett, SA Labuzan, I Frankenhau, NO Azu, AM Liesinger, ER Lesser, DJ Serie, ZS Quicksall, TA Patel, TP Carnwath, M DeTure, X Tang, RC Petersen, R Duara, NR Graff-Radf, M Allen, MM Carrasquil, H Li, OA Ross, N Ertekin-Ta, DW Dickson, YW Asmann, RE Carter, ME Murray Transcriptomic analysis to identify genes associated with selective hippocampal vulnerability in Alzheimer's disease Nature Communications, 2021;12(1):2311. 2021-01-01 [PMID: 33875655] (Co-IP, Human)
• Prohaska TA, Wahlmuller FC, Furtmuller M, Geiger M Interaction of protein C inhibitor with the type II transmembrane serine protease enteropeptidase. PLoS ONE, 2012;7(6):e39262. 2012-01-01 [PMID: 22723979] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SERPINA5
• Uniprot:
  • P05154()