ACE-2 Antibody [Biotin]

ACE-2 was detected in immersion fixed paraffin-embedded sections of human kidney using Human ACE-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

ACE-2 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ACE-2
  Gln18-Ser740
  Accession # Q9BYF1
• Specificity: Detects human ACE-2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human ACE is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACE2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ACE-2 Antibody [Biotin]

• ACE2
• ACE-2
• ACEH
• ACEHangiotensin I converting enzyme 2
• ACE-related carboxypeptidase
• angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• angiotensin-converting enzyme 2
• Angiotensin-converting enzyme homolog
• DKFZp434A014
• EC 3.4.17
• EC 3.4.17.23
• Metalloprotease MPROT15

Background

ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).

1. Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
2. Crackower,  M.A. et al. (2002) Nature 417:822.
3. Huang, L. et al. (2003) J. Biol. Chem. 278:15532.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACE-2 Antibody (BAF933)(10)

Publications using BAF933

• Lauren Grimm, Chinyere Onyeukwu, Grace Kenny, Danielle M. Parent, Jia Fu, Shaurya Dhingra et al. Immune Dysregulation in Acute SARS-CoV-2 Infection Pathogens and Immunity 2023-02-20 [PMID: 36865568]
• RH Shoemaker, RA Panettieri, SK Libutti, HS Hochster, NR Watts, PT Wingfield, P Starkl, L Pimenov, R Gawish, A Hladik, S Knapp, D Boring, JM White, Q Lawrence, J Boone, JD Marshall, RL Matthews, BD Cholewa, JW Richig, BT Chen, DL McCormick, R Gugensberg, S Höller, JM Penninger, G Wirnsberge Development of an aerosol intervention for COVID-19 disease: Tolerability of soluble ACE2 (APN01) administered via nebulizer PLoS ONE, 2022-07-11;17(7):e0271066. 2022-07-11 [PMID: 35816490] (ELISA Capture, Human)
• Brian Dobosh, Keivan Zandi, Diego Moncada Giraldo, Shu Ling Goh, Kathryn Musall, Milagros Aldeco et al. Baricitinib attenuates the proinflammatory phase of COVID-19 driven by lung-infiltrating monocytes Cell Reports 2022-06-01 [PMID: 35688145]
• V Vásquez, MC Navas, JA Jaimes, J Orozco SARS-CoV-2 electrochemical immunosensor based on the spike-ACE2 complex Analytica chimica acta, 2022-03-23;1205(0):339718. 2022-03-23 [PMID: 35414393] (ELISA Development, Human)
• H Caohuy, O Eidelman, T Chen, S Liu, Q Yang, A Bera, NI Walton, TT Wang, HB Pollard Common cardiac medications potently inhibit ACE2 binding to the SARS-CoV-2 Spike, and block virus penetration and infectivity in human lung cells Scientific Reports, 2021-11-12;11(1):22195. 2021-11-12 [PMID: 34773067] (ELISA Detection, Human)
• RH Shoemaker, RA Panettieri, SK Libutti, HS Hochster, NR Watts, PT Wingfield, P Starkl, L Pimenov, R Gawish, A Hladik, S Knapp, D Boring, JM White, Q Lawrence, J Boone, JD Marshall, RL Matthews, BD Cholewa, JW Richig, BT Chen, DL McCormick, R Gugensberg, S Höller, JM Penninger, G Wirnsberge Development of a novel, pan-variant aerosol intervention for COVID-19 bioRxiv : the preprint server for biology, 2021-09-20;0(0):. 2021-09-20 [PMID: 34545364] (ELISA Detection, Human)
• Kazuhito Honjo, Ronnie M. Russell, Ran Li, Weimin Liu, Regina Stoltz, Edlue M. Tabengwa et al. Convalescent plasma-mediated resolution of COVID-19 in a patient with humoral immunodeficiency Cell Reports Medicine 2021-01-01 [PMID: 33521696] (ELISA Detection, Human)
• Rodolfo Mattar Rosa, Juliana Almada Colucci, Rodrigo Yokota, Roseli Peres Moreira, Danielle Sanches Aragão, Amanda Aparecida Ribeiro et al. Alternative pathways for angiotensin II production as an important determinant of kidney damage in endotoxemia American Journal of Physiology-Renal Physiology 2016-09-01 [PMID: 27252489]
• Zon W. Lai, Rebecca A. Lew, Michael A. Yarski, Fi-Tjen Mu, Robert K. Andrews, A. Ian Smith The Identification of a Calmodulin-Binding Domain within the Cytoplasmic Tail of Angiotensin-Converting Enzyme-2 Endocrinology 2009-05-01 [PMID: 19164471]
• Follis KE, York J, Nunberg JH Furin cleavage of the SARS coronavirus spike glycoprotein enhances cell-cell fusion but does not affect virion entry. Virology, 2006-03-07;350(2):358-69. 2006-03-07 [PMID: 16519916] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ACE2
• Uniprot:
  • Q9BYF1()