ACE-2 Proteins

933-ZN were tested in a functional ELISA for binding to recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein 10499-CV. All four lots were plotted on the same graph to show lot-to-lot consistency of recombinant Human ACE-2. The ED₅₀ for this effect is 1.00-12.0 ng/mL." class="big_lightbox"> 933-ZN were tested in a functional ELISA for binding to recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein 10499-CV. All four lots were plotted on the same graph to show lot-to-lot consistency of recombinant Human ACE-2. The ED₅₀ for this effect is 1.00-12.0 ng/mL." class="big_thumb" />

10499-CV) was immobilized on a Biacore Sensor Chip CM5, and binding to recombinant human ACE-2 (933-ZN) was measured at a concentration range between 0.73 nM and 46.7 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of K_d=7.037 nM. (Biacore T200)." class="big_lightbox"> 10499-CV) was immobilized on a Biacore Sensor Chip CM5, and binding to recombinant human ACE-2 (933-ZN) was measured at a concentration range between 0.73 nM and 46.7 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of K_d=7.037 nM. (Biacore T200)." class="big_thumb" />

ES007)." class="big_lightbox"> ES007)." class="big_thumb" />

10544-ZN) binds Recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein (10499-CV) in a functional ELISA." class="big_lightbox"> 10544-ZN) binds Recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein (10499-CV) in a functional ELISA." alt="Recombinant Human ACE-2 Fc Chimera (10544-ZN) binds Recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein (10499-CV) in a functional ELISA." class="big_thumb" />

10544-ZN) is measured by its ability to cleave fluorogenic peptide substrate Mca-YVADAPK(Dnp)-OH (ES007)" class="big_lightbox"> 10544-ZN) is measured by its ability to cleave fluorogenic peptide substrate Mca-YVADAPK(Dnp)-OH (ES007)" class="big_thumb" />

10544-ZN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~126 kDa under reducing conditions." class="big_lightbox"> 10544-ZN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~126 kDa under reducing conditions." class="big_thumb" />

10499-CV) in a functional ELISA." class="big_lightbox"> 10499-CV) in a functional ELISA." alt="Biotinylated Recombinant Human ACE-2 His-tag (Catalog # BT933) binds Recombinant SARS-CoV-2 Spike RBD Fc Chimera (10499-CV) in a functional ELISA." class="big_thumb" />

ES007). " class="big_lightbox"> ES007). " class="big_thumb" />

ES007)." class="big_lightbox"> ES007)." alt="Recombinant Mouse ACE-2 His-tag (Catalog # 3437-ZN) is measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007)." class="big_thumb" />

ES007). " class="big_lightbox"> ES007). " alt="Recombinant Cynomolgus Monkey ACE‑2 His-tag Protein (Catalog # 10619-ZN) is measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (ES007). " class="big_thumb" />

10499-CV) in a functional ELISA." class="big_lightbox"> 10499-CV) in a functional ELISA." class="big_thumb" />

ES007). " class="big_lightbox"> ES007). " alt="Biotinylated Recombinant Human ACE‑2 Fc-Chimera (Catalog # BT10544) is measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (ES007). " class="big_thumb" />

10499-CV) in a functional ELISA." class="big_lightbox"> 10499-CV) in a functional ELISA." class="big_thumb" />

10499-CV) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human ACE-2 Fc Chimera Avi-tag (CHO Expressed) (Catalog # AVI10544) binds with an ED₅₀ of 0.6-4.8 ng/mL." class="big_lightbox"> 10499-CV) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human ACE-2 Fc Chimera Avi-tag (CHO Expressed) (Catalog # AVI10544) binds with an ED₅₀ of 0.6-4.8 ng/mL." alt="When Recombinant SARS-CoV-2 Spike RBD Fc Chimera (10499-CV) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human ACE-2 Fc Chimera Avi-tag (CHO Expressed) (Catalog # AVI10544) binds with an ED₅₀ of 0.6-4.8 ng/mL." class="big_thumb" />

ES007). " class="big_lightbox"> ES007). " class="big_thumb" />

10499-CV) in a functional ELISA." class="big_lightbox"> 10499-CV) in a functional ELISA." alt="Recombinant Hamster ACE-2 His-tag (Catalog # 10578-ZN) binds Recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein (10499-CV) in a functional ELISA." class="big_thumb" />

ES007). " class="big_lightbox"> ES007). " class="big_thumb" />

10499-CV) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human ACE-2 His-tag Avi-tag (CHO Expressed) (Catalog # AVI10579) binds with an ED₅₀ of 1.2-9.6 ng/mL." class="big_lightbox"> 10499-CV) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human ACE-2 His-tag Avi-tag (CHO Expressed) (Catalog # AVI10579) binds with an ED₅₀ of 1.2-9.6 ng/mL." alt="When Recombinant SARS-CoV-2 Spike RBD Fc Chimera (10499-CV) is immobilized at 0.2 µg/mL (100 µL/well), Biotinylated Recombinant Human ACE-2 His-tag Avi-tag (CHO Expressed) (Catalog # AVI10579) binds with an ED₅₀ of 1.2-9.6 ng/mL." class="big_thumb" />

ES007). " class="big_lightbox"> ES007). " class="big_thumb" />

10545-ZN) is measured by its ability to cleave fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (ES007)" class="big_lightbox"> 10545-ZN) is measured by its ability to cleave fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (ES007)" alt="Recombinant Porcine ACE-2 His-tag (10545-ZN) is measured by its ability to cleave fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (ES007)" class="big_thumb" />

10545-ZN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~111 kDa under reducing conditions." class="big_lightbox"> 10545-ZN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~111 kDa under reducing conditions." class="big_thumb" />

ES007). " class="big_lightbox"> ES007). " alt="Recombinant Canine ACE-2 His-tag (Catalog # 10566-ZN) is measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (ES007). " class="big_thumb" />

ES007). " class="big_lightbox"> ES007). " alt="Recombinant Ferret ACE‑2 His-tag Protein (Catalog # 10635-ZN) is measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (ES007). " class="big_thumb" />