Mas Antibody

Images

 
Western Blot: Mas Antibody [NBP1-78444] - Analysis of MAS1 in: 1. Ntera 2, 2. A431, 3. HepG2, 4. MCF7, 5. 3T3 and 6. Cos7.
Immunocytochemistry/ Immunofluorescence: Mas Antibody [NBP1-78444] - MAS1 antibody was tested at 1:25 in HepG2 cells with FITC (green). Nuclei were counterstained with Dapi (blue).
Immunohistochemistry: Mas Antibody [NBP1-78444] - Analysis of MAS1 in mouse kidney using DAB with hematoxylin counterstain.
Simple Western: Mas Antibody [NBP1-78444] - Simple Western lane view shows a specific band for MAS1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Mas Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human MAS1 protein (between residues 75-125) [UniProt P04201]
Localization
Cell membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:25
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This MAS1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin embedded sections and Western blot.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-78444 in the following applications:

Publications
Read Publications using
NBP1-78444 in the following applications:

Reactivity Notes

Human, monkey, rat, and mouse. Rabbit reactivity reported in the scientific literature (PMID: 23701246).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Mas Antibody

  • Mas
  • MAS1 oncogene
  • MAS1
  • MGC119966
  • proto-oncogene Mas

Background

MAS1 oncogene (Proto-oncogene Mas, MAS1) is a GTP binding protein coupled receptor which shares a conserved structural motif with other proteins of its class. When activated, MAS1 is a modulator of a critical step in the growth-regulating pathway to trigger oncogenes (PMID: 20674894). Evidence has shown that the MAS1 oncogene acts as a receptor for angiotensin (Ang)-(1-7), however it is not fully known how Intracellular signaling through Mas1 occurs (PMID: 19461648). Furthermore, research proves that in tandom with Ang-(1-7), the MAS1 receptor is expressed in the ovarian stroma of post menopausal women and in male testis (PMID: 20674894, 20361351). Additionally, together with Ang- (1-7) MAS1 may be an important component of normal spermatogenesis as low expression levels were found in infertile men with impaired spermatogenesis (PMID: 20361351).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Mas Antibody (NBP1-78444)(4)

Reviews for Mas Antibody (NBP1-78444) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-78444:
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WB
(1)
IF
(1)
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Human
(1)
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot Mas NBP1-78444
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reviewed by:
Kasia Szymanska
WB Human 10/30/2013
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Summary

ApplicationWestern Blot
Sample TestedIMCD3, hTertRPE1, HDF, SHSY5Y whole cell lysates
SpeciesHuman
LotA-1

Blocking

Blocking Details10% non-fat milk in PBST, 2h, at room temp

Primary Anitbody

Dilution Ratio1:1000 in 100on-fat milk/PBST, O/N, at 4deg C

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#Dako
Secondary Concentration1:10000
Immunofluorescence Mas NBP1-78444
Enlarge
reviewed by:
Gabrielle Wheway
IF Mouse 10/14/2013
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse inner medullary collecting duct (IMCD3) cell line
SpeciesMouse
LotA-1

Blocking

Blocking Details1% non-fat milk/PBS 15 minutes room temperature

Primary Anitbody

Dilution Ratio1:100 1 hour room temperature

Secondary Antibody

Secondary Descriptiongoat anti rabbit IgG Alexa Fluor 488
Secondary Concentration1:1000

Details

Fixation Detailsmethanol fixation -20oC 5 minutes
Wash Description3 x 5 minutes PBS

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Mas Antibody (NBP1-78444). (Showing 1 - 1 of 1 FAQs).

  1. Hi, I have two questions about MAS1 Antibody (NBP1-78444). Could you tell me if it could detect N-terminal tag3* flag-Mas or not? The human N-terminal tag3* flag-Mas vector that I constructed were veri?ed by DNA sequencing and were consistent with the NCBI reference sequence: NM_002377.2. It's open reading frame-shift did not appear and MAS1 is about 34KD. Why is the MW of MAS1 is bigger than 37KD on your datasheet?
    • I have pulled up information on your NCBI sequence and it matches that associated with the uniprot ID for this antibody: P04201. The protein appears to theoretically have a molecular weight of 37 kDa, but most likely due to post translational modifications such as glycosylation we expressed the protein at a higher molecular weight in our sample, which can be common for proteins that under go post translational modifications such as this one. We will back this antibody with our 100% guarantee for all stated species and applications.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-78444AF405
Alexa Fluor 488 Labeled NBP1-78444AF488
Alexa Fluor 647 Labeled NBP1-78444AF647
Alexa Fluor 700 Labeled NBP1-78444AF700
Biotin Labeled NBP1-78444B
DyLight 405 Labeled NBP1-78444V
DyLight 488 Labeled NBP1-78444G
DyLight 550 Labeled NBP1-78444R
DyLight 650 Labeled NBP1-78444C
FITC Labeled NBP1-78444F
HRP Labeled NBP1-78444H

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Recent Reviews

5
5
2
4
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2
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1
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Kasia Szymanska
10/30/2013
Application: WB
Species: Human

Gabrielle Wheway
10/14/2013
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol MAS1
Entrez
Uniprot