Mas Antibody - BSA Free

Images

 
Western Blot: Mas Antibody [NBP1-78444] - Analysis of MAS1 in: (1) Ntera 2, (2) A431, (3) HepG2, (4) MCF7, (5) 3T3, and (6) Cos7.
Immunocytochemistry/ Immunofluorescence: Mas Antibody [NBP1-78444] - Tested at 1:25 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: Mas Antibody [NBP1-78444] - Analysis of MAS1 in mouse kidney using DAB with hematoxylin counterstain.
Simple Western: Mas Antibody [NBP1-78444] - Lane view shows a specific band for MAS1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Mas Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal portion of the human MAS1 protein (between residues 75-125) [Uniprot: P04201]
Localization
Cell membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:25
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Simple Western 1:100
  • Western Blot 1:1000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-78444 in the following applications:

Publications
Read Publications using
NBP1-78444 in the following applications:

  • 2 publications
  • IHC
    2 publications
  • 1 publication
  • WB
    12 publications

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34558543). Rabbit reactivity reported in the scientific literature (PMID: 23701246).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Mas Antibody - BSA Free

  • Mas
  • MAS1 oncogene
  • MAS1
  • MGC119966
  • proto-oncogene Mas

Background

MAS1 oncogene (Proto-oncogene Mas, MAS1) is a GTP binding protein coupled receptor which shares a conserved structural motif with other proteins of its class. When activated, MAS1 is a modulator of a critical step in the growth-regulating pathway to trigger oncogenes (PMID: 20674894). Evidence has shown that the MAS1 oncogene acts as a receptor for angiotensin (Ang)-(1-7), however it is not fully known how Intracellular signaling through Mas1 occurs (PMID: 19461648). Furthermore, research proves that in tandom with Ang-(1-7), the MAS1 receptor is expressed in the ovarian stroma of post menopausal women and in male testis (PMID: 20674894, 20361351). Additionally, together with Ang- (1-7) MAS1 may be an important component of normal spermatogenesis as low expression levels were found in infertile men with impaired spermatogenesis (PMID: 20361351).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP3-15868
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-30027
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-47974
Species: Ca, Hu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-87581
Species: Hu
Applications: IHC, IHC-P, WB
AF933
Species: Ha, Hu, Mu, Rt
Applications: Block, Flow, IHC, IP, Simple Western, WB
DAN00
Species: Hu
Applications: ELISA
AF1513
Species: Mu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
NBP2-46376
Species: Hu
Applications: IHC, WB
NBP2-45519
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-47801
Species: Ca, Hu, Pm, Mu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-77078
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
AF4090
Species: Hu
Applications: IHC, IP, Neut, WB
NBP1-32870
Species: Ch, Hu
Applications: IHC, IHC-P, IP, KD, WB
NBP2-43648
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-58268
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NB200-541
Species: Mu
Applications: IA, ICC/IF, IHC, IHC-Fr, IP, WB
H00008458-M06
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, WB
NBP2-67656
Species: Hu, Mu, Rt, Ze
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-48733
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP1-78444
Species: Hu, Mu, Rt, Pm, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for Mas Antibody (NBP1-78444)(16)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IHC
(2)
IHC-P
(1)
WB
(12)
All Applications
Filter By Species
Human
(2)
Mouse
(5)
Rat
(7)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NBP1-78444 Applications Species
Gao Q, Chen R, Wu L et al. Angiotensin-(1-7) reduces alpha -synuclein aggregation by enhancing autophagic activity in Parkinson's disease Neural regeneration research May 1 2022 [PMID: 34558543] (WB, Rat) WB Rat
Dang R, Yang M, Cui C et al. Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling Aging cell Sep 16 2021 [PMID: 34529881] (IHC-P, WB, Mouse) IHC-P, WB Mouse
Yoshihara T, Deminice R, Hyatt H et al. Angiotensin 1-7 protects against ventilator-induced diaphragm dysfunction Clinical and translational science Mar 20 2021 [PMID: 33742769] (WB, Rat) WB Rat
Fan H, Bhullar K, Wu J Spent Hen Muscle Protein-Derived RAS Regulating Peptides Show Antioxidant Activity in Vascular Cells Antioxidants Feb 15 2021 [PMID: 33671990] (WB, Human, Rat) WB Human, Rat
Dang Z, Su S, Jin G, et al. Tsantan Sumtang attenuated chronic hypoxia-induced right ventricular structure remodeling and fibrosis by equilibrating local ACE-AngII-AT1R/ACE2-Ang1-7-Mas axis in rat J Ethnopharmacol Dec 17 2019 [PMID: 31862407] (IHC, Rat) IHC Rat
Stone RE, Liu S, Levy AM, et al. Activation of the Protective Arm of the Renin Angiotensin System in Demyelinating Disease J Neuroimmune Pharmacol Dec 11 2019 [PMID: 31828731]
Cerniello FM, Silva MG, Carretero OA, Gironacci MM Mas receptor is translocated to the nucleus upon agonist stimulation in brainstem neurons from spontaneously hypertensive rats but not normotensive rats Cardiovasc. Res. Dec 11 2019 [PMID: 31825460] (WB, Rat) WB Rat
Cui C, Xu P, Li G et al. Vitamin D receptor activation regulates microglia polarization and oxidative stress in spontaneously hypertensive rats and angiotensin II-exposed microglial cells: Role of renin-angiotensin system Redox Biol Aug 8 2019 [PMID: 31421410] (WB, Mouse) WB Mouse
Mo J, Enkhjargal B, Travis ZD et al. AVE 0991 attenuates oxidative stress and neuronal apoptosis via Mas/PKA/CREB/UCP-2 pathway after subarachnoid hemorrhage in rats Redox Biol. 2018 Sep 27 [PMID: 30296700] (WB, ICC/IF, Rat) WB, ICC/IF Rat
Ma X, Pang Z, Zhou J et al. Acetylation and Amination Protect Angiotensin 1-7 from Physiological Hydrolyzation and Therefore Increases Its Antitumor Effects on Lung Cancer Mol. Pharm. Apr 27 2018 [PMID: 29685037] (WB, Mouse) WB Mouse
Show All 16 Publications.

Reviews for Mas Antibody (NBP1-78444) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-78444:
Filter by Applications
Western Blot
(1)
Immunofluorescence
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Mas NBP1-78444
Enlarge
5
reviewed by:
Verified Customer
Western Blot Human 10/30/2013
View

Summary

ApplicationWestern Blot
Sample TestedIMCD3, hTertRPE1, HDF, SHSY5Y whole cell lysates
SpeciesHuman
LotA-1
Immunofluorescence Mas NBP1-78444
Enlarge
5
reviewed by:
Verified Customer
Immunofluorescence Mouse 10/14/2013
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse inner medullary collecting duct (IMCD3) cell line
SpeciesMouse
LotA-1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Mas Antibody (NBP1-78444). (Showing 1 - 1 of 1 FAQs).

  1. Hi, I have two questions about MAS1 Antibody (NBP1-78444). Could you tell me if it could detect N-terminal tag3* flag-Mas or not? The human N-terminal tag3* flag-Mas vector that I constructed were veri?ed by DNA sequencing and were consistent with the NCBI reference sequence: NM_002377.2. It's open reading frame-shift did not appear and MAS1 is about 34KD. Why is the MW of MAS1 is bigger than 37KD on your datasheet?
    • I have pulled up information on your NCBI sequence and it matches that associated with the uniprot ID for this antibody: P04201. The protein appears to theoretically have a molecular weight of 37 kDa, but most likely due to post translational modifications such as glycosylation we expressed the protein at a higher molecular weight in our sample, which can be common for proteins that under go post translational modifications such as this one. We will back this antibody with our 100% guarantee for all stated species and applications.

Secondary Antibodies

 

Isotype Controls

Additional Mas Products

Array NBP1-78444

Bioinformatics Tool for Mas Antibody (NBP1-78444)

Discover related pathways, diseases and genes to Mas Antibody (NBP1-78444). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Mas Antibody (NBP1-78444)

Discover more about diseases related to Mas Antibody (NBP1-78444).
 

Pathways for Mas Antibody (NBP1-78444)

View related products by pathway.

PTMs for Mas Antibody (NBP1-78444)

Learn more about PTMs related to Mas Antibody (NBP1-78444).
 

Research Areas for Mas Antibody (NBP1-78444)

Find related products by research area.

Blogs on Mas

There are no specific blogs for Mas, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Verified Customer
10/30/2013
Application: Western Blot
Species: Human

Verified Customer
10/14/2013
Application: Immunofluorescence
Species: Mouse

Bioinformatics

Gene Symbol MAS1
Entrez
Uniprot