Western Blot: Mas Antibody [NBP1-78444] - Analysis of MAS1 in: (1) Ntera 2, (2) A431, (3) HepG2, (4) MCF7, (5) 3T3, and (6) Cos7.
Immunocytochemistry/ Immunofluorescence: Mas Antibody [NBP1-78444] - Tested at 1:25 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: Mas Antibody [NBP1-78444] - Analysis of MAS1 in mouse kidney using DAB with hematoxylin counterstain.
Simple Western: Mas Antibody [NBP1-78444] - Lane view shows a specific band for MAS1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Use in Rat reported in scientific literature (PMID:34558543). Rabbit reactivity reported in the scientific literature (PMID: 23701246).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for Mas Antibody - BSA Free
MAS1 oncogene (Proto-oncogene Mas, MAS1) is a GTP binding protein coupled receptor which shares a conserved structural motif with other proteins of its class. When activated, MAS1 is a modulator of a critical step in the growth-regulating pathway to trigger oncogenes (PMID: 20674894). Evidence has shown that the MAS1 oncogene acts as a receptor for angiotensin (Ang)-(1-7), however it is not fully known how Intracellular signaling through Mas1 occurs (PMID: 19461648). Furthermore, research proves that in tandom with Ang-(1-7), the MAS1 receptor is expressed in the ovarian stroma of post menopausal women and in male testis (PMID: 20674894, 20361351). Additionally, together with Ang- (1-7) MAS1 may be an important component of normal spermatogenesis as low expression levels were found in infertile men with impaired spermatogenesis (PMID: 20361351).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Le Nguyen B, Yoshihara T, Deminice R et al. Alterations in renin-angiotensin receptors are not responsible for exercise preconditioning of skeletal muscle fibers Sports Medicine and Health Science Jul 1 2021 (WB, Rat)
FAQs for Mas Antibody (NBP1-78444). (Showing 1 - 1 of 1 FAQs).
Hi, I have two questions about MAS1 Antibody (NBP1-78444). Could you tell me if it could detect N-terminal tag3* flag-Mas or not? The human N-terminal tag3* flag-Mas vector that I constructed were veri?ed by DNA sequencing and were consistent with the NCBI reference sequence: NM_002377.2. It's open reading frame-shift did not appear and MAS1 is about 34KD. Why is the MW of MAS1 is bigger than 37KD on your datasheet?
I have pulled up information on your NCBI sequence and it matches that associated with the uniprot ID for this antibody: P04201. The protein appears to theoretically have a molecular weight of 37 kDa, but most likely due to post translational modifications such as glycosylation we expressed the protein at a higher molecular weight in our sample, which can be common for proteins that under go post translational modifications such as this one. We will back this antibody with our 100% guarantee for all stated species and applications.
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