ACE-2 Antibodies: Novus Biologicals

AF933

ACE-2 Antibody [Unconjugated]

(1 Review)

100 μg

AF933

HAF017). A specific band was detected for ACE-2 at approximately 100 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human kidney tissue, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for ACE-2 at approximately 100 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of human kidney tissue, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ACE-2 at approximately 100 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

F0108). Quadrant markers were set based on Goat IgG control antibody (AB-108-C, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox">

HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF933) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (<a class=

F0108). Quadrant markers were set based on Goat IgG control antibody (AB-108-C, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

ACE-2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to respiratory bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

ACE‑2 was detected in immersion fixed paraffin-embedded sections of hamster lung using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to respiratory bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in eputhelial cells in bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

ACE‑2 was detected in immersion fixed frozen sections of rat lung using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in eputhelial cells in bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

ACE‑2 was detected in immersion fixed paraffin-embedded sections of rat lung using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

ACE‑2 was detected in immersion fixed paraffin-embedded sections of rat kidney using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE-2 at approximately 155 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

(A) Transduction of pLV pseudotyped with VSV-G (A,C) or CoV-2 Spike glycoprotein (B,D) in HEK293T (A,B) or Vero E6 (C,D) cells. The lentiviral backbone incorporates enhanced green fluorescent protein (eGFP) that is expressed upon integration into target cells. The fluorescence was recorded at 48 h post transduction. Magnification 4X. (E) Transduction efficiency of pLV pseudotyped with CoV-2 Spike glycoprotein in Vero E6, hACE2-HEK293T and 293T cells. The fluorescence was recorded at 48 h post transduction. The experiments were done in triplicates and standard error of mean was plotted as error bars. (F) Whole cell lysates from Vero E6, hACE2-293T and 293T cells were run on SDS-PAGE and probed with anti ACE2 antibody. Beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33154514), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunoblot analysis of ACE2 protein in the media from high glucose- or Ang II-stimulated mouse PT cells.(A) Mouse PT cells were incubated for 72 hrs in normal media (C, 7.8 mM D-glucose), with or without Ang II (10−7 M), high D-glucose (D-G, 25 mM), or high L-glucose (25 mM). Above graph is representative immunoblot for ACE2 in the media, showing bands at ∼90 kDa and ∼70 kDa. (B) Graphical representation of densitometry analysis of two ACE2 bands on immunoblots. For the ∼90 kDa band, *p<0.05 vs C, **p<0.001 vs C, **p<0.003 vs L-G; n = 5. For the ∼70 kDa band, *p<0.04 vs C; **p<0.001 vs C or L-G, **p<0.03 vs Ang II; n = 5. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0085958), licensed under a CC-BY license. Not internally tested by R&D Systems.

$<b>Genetic Strategies Validation. </b>Deglycosylation of ACE2 protein in media and cell lysates from mouse PT cells.Representative immunoblot for ACE2 treated without (−) or with (+) deglycosylation with PNGase F in the media (Lanes 1–2) and cell lysates (Lanes 3–4). Lanes 1 and 3: wildtype PT cells, Lanes 2 and 4: ACE2 knockout (KO) PT cells transfected with a human ACE2 vector, Lane 5: mouse kidney cortex. Lanes 1+ and 2+ show a reduction in the sizes of ACE2 fragments in media fractions to ∼75 kDa and ∼60 kDa for mouse ACE2, and to ∼80 kDa and ∼65 kDa for human ACE2, respectively. Lanes 3+ and 4+ show a reduction in the sizes of ACE2 in cell lysates to ∼85 kDa for both mouse and human ACE2 treated with the PNGase F, respectively. Lane 5+ shows a reduction in size of ACE2 in mouse cortex from ∼100 kDa to ∼85 kDa after treatment with PNGase F. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0085958), licensed under a CC-BY license. Not internally tested by R&D Systems.$

<b>Genetic Strategies Validation. </b>Immunoblot analysis of ACE2 protein in media and cell lysates from mouse PT cells.(A) Representative immunoblot for ACE2 protein in concentrated media (Lanes 1–3) and cell lysates (Lanes 4–6) from mouse PT cells. Lanes 1 and 4: wildtype cells, Lanes 2 and 5: ACE2 knockout (KO) cells, Lanes 3 and 6: ACE2 KO cells transfected with a human ACE2 expression vector, Lane 7: mouse kidney cortex showing a band at ∼100 kDa, used as a positive control. Lane 1 shows two bands in the media at ∼90 kDa and ∼70 kDa for mouse ACE2. Lane 3 shows two bands in the media for human ACE2 in transfected cells, at ∼110 kDa and ∼95 kDa. Lanes 4 and 6 show a single band in cell lysates at ∼100 kDa for mouse ACE2, and ∼120 kDa for human ACE2, respectively. Lanes 2 and 5 show no ACE2 bands detected on immunoblots of both media and cell lysates from untransfected ACE2 KO cells. (B) Increased ACE2 activity in the media from ACE2 KO cells transfected with a human ACE2 expression vector (HA-hACE2, 3.75 µg on 35 mm culture dishes). Untransfected cells and cells transfected with an empty pcDNA3 vector had no detectable ACE2 activity in the media. Numbers in parentheses represent mean values for ACE2 activity. *P<0.001 vs untransfected control or empty pcDNA3 vector, n = 4. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0085958), licensed under a CC-BY license. Not internally tested by R&D Systems.

Simple Western: ACE-2 Antibody [Unconjugated] [AF933] - Design for a membrane-localized ACE2 expression system. (A) Our ACE2 construct is driven by a CMV promoter followed by the first 25 residues of ACE2 containing the leader sequence that direct ACE2 to the plasma membrane. This is followed by a 3xHA tag linked to the remainder of ACE2 (20-805) and a C-terminal sfGFP. Both 3xHA and sfGFP fusions are separated from ACE2 by flexible 3xGGGGS linkers. (B) The ACE2 fusion protein is designed to be embedded in the plasma membrane where it can perform extracellular carboxypeptidase-mediated metabolism and its levels can be detected by cell staining with antibodies to HA. (C) Lysates from untransfected or 3xHA-ACE2-sfGFP-transfected HEK293 cells were analyzed by automated Jess capillary immunoassay using antibodies to HA, GFP, and two ACE2 antibodies. (D) Confocal fluorescence microscopy of HEK cells transfected with 3xHA-ACE2-sfGFP and stained with HA and the nuclear stain DAPI. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37644110), licensed under a CC-BY license. Not internally tested by R&D Systems.

Distribution of ACE2 along the olfactory retrograde route. A Schematic elucidation of a gross view of mouse olfactory retrograde route comprised by OB surface, SEZ/RC, SEZ in the rostral migratory stream (SEZ/RMS) and SVZ of the LV (top panel) and a microscopic view of SEZ/RC and SEZ/RMS (bottom panel). B–E Co-staining of ACE2 (green) with astrocyte marker GFAP (red) in SEZ/RC and SEZ/RMS. B-C show the low magnitude images. D–E show the enlarged images of the indicated regions in B and C, respectively. DAPI (blue) was used for nuclear staining. Arrow: indicates astrocyte. Dash arrow: indicates neuroblast. Arrowhead: indicates microvessel. The dash lines in all images outline the border of SEZ/RC and SEZ/RMS, respectively. Image magnitude B-C: 20X, D-E: 100X Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35672716), licensed under a CC-BY license. Not internally tested by R&D Systems.

Ubiquitous expression of ACE2 in cerebral microvascular pericytes. A Schematic elucidation of sectioning plates and main brain regions of a mouse brain. OB: olfactory bulb, SEZ/RC: subependymal zone (SEZ) in the rhinocele, CTX: cerebral cortex, CC: corpus callosum, STR: striatum, LV: lateral ventricle, HP: hippocampus, TH: thalamus, 3 V: third ventricle, HY: hypothalamus, AQ: cerebral aqueduct, MB: midbrain, CB: cerebellum, 4 V: fourth ventricle, MY: medulla oblongata. B Scanning images show ACE2 (green) distribution in the hemisphere sections. C–F ACE2 (red) ubiquitously distributes in the cerebral microvessels labeled by endothelial cell marker CD31 (green) and pericyte marker PDGFR beta (green), respectively. ACE2 overlapped with pericyte marker PDGFR beta (F) but not endothelial cell marker CD31 (D). C and E showed the low magnitude images. D and F showed the enlarged images of the indicated regions in C and E, respectively. G ACE2 (red) does not distribute in large blood vessels marked by CD31 (green) but is detectable in the meninges wrapping HY. DAPI (blue) was used for nuclear staining. *: indicates large blood vessels. #: indicates meninges. Arrowhead: indicates microvessel. Image magnitude B: 10X; C, E and G: 20X; D and F: 100X Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35672716), licensed under a CC-BY license. Not internally tested by R&D Systems.

Differential distribution of ACE2 in brain parenchyma. A Uneven distribution of ACE2 (green) in indicated brain regions. Each insert shows a comparable high magnitude image of the corresponding region. B Enlarged images of the indicated region in the MY panel in A with co-staining of ACE2 (green) and astrocyte marker GFAP (red). C Co-staining of ACE2 (red) and neuron marker NeuN (green) in the MY. Cii-Civ show the high magnitude image of the comparable regions of Ci. D Quantitation of the relative expression of ACE2 in astrocytes and neurons. Values are presented as mean ± SEM. n = 60. ***: p < 0.001. DAPI (blue) was used for nuclear staining. Arrowhead: indicates microvessel. Arrow: indicates astrocyte. Dash arrow: indicates neuron. #indicates meninges wrapping MY. Image magnitude A and Ci: 20X, B and Cii-iv: 100X Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35672716), licensed under a CC-BY license. Not internally tested by R&D Systems.

ADAM17 deletion reduces ACE2 shedding in post-MI mice. Knockout of ADAM17 in the myocardium of MI mice using TAPI-1, the same as before. A Western blot analysis of the expression of ADAM17, p-ADAM17, pP38MAPK, total P38MAPK, ACE2 protein in different position myocardium of 30 days post-MI mice. B Quantification of pP38MAPK levels in different position of myocardium. C Quantification of ACE2 levels in cardiomyocytes. D Detection of ACE2 activity in cardiomyocyte lysates by ACE2 activity fluorometric assay. E Detection of pADAM17 Thr735 expression in peripheral blood of patients with or without HF after MI. F Detection of ACE2 expression in peripheral blood of patients with or without HF after MI. N = 6 biological replicates. Data shown as mean ± SEM. Derived by two-sample t-test, *P < 0.05; **P < 0.01, ***P < 0.001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37046278), licensed under a CC-BY license. Not internally tested by R&D Systems.

Activated P38MAPK induced phosphorylation of ADAM17 in injured cardiomyocyte. A–C Representative western blot (A) and quantitative results depicting the phosphorylation vs. total protein levels of P38 (B) and ERK1/2 (C) in H9C2 cardiomyocytes treated with 600 μM hydrogen peroxide for 12 h (n = 3). D Phosphorylated ADAM17 (p-ADAM17) was assessed by western blot with anti-phospho-ADAM17 (pThr735) antibody after transfection with ADAM17 siRNA or P38MAPK inhibitor SB203580 as indicated (n = 3). E Flow cytometric analysis of annexin V/PI double stained cardiomyocyte after treatment as indicated (right). Histogram indicating the percentage of later apoptosis cells in 4 groups (left) (n = 3). F The cell viability was detected by CCK8 assay (n = 4). G The live (green) and dead (red) cells were observed by calcein-AM/PI double staining kit after treatment as indicated (right). Histogram showing percentage of viable cells(left) (n = 3). H Protein levels of ACE2 (110 kDa), collagen I, collagen III TGF-beta 1 in cardiomyocytes and released ACE2 protein (90 kDa) in the supernatant were detected by western blotting(n = 3). I, J ACE2 activity in cardiomyocyte lysates (I) and cardiomyocyte supernatant (J) was detected by the ACE2 Activity Fluorometric Assay(n = 4). Data shown as mean ± SEM. Derived by two-sample t-test, *P < 0.05; **P < 0.01, ***P < 0.001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37046278), licensed under a CC-BY license. Not internally tested by R&D Systems.

Increased expression of TMPRSS2 and ACE2 by Vero E6-TMPRSS2-T2A-ACE2 cells. (A) Analysis of TMPRSS2 mRNA levels by qRT-PCR. mRNA levels (-fold) in Vero E6, VeroE6/TMPRSS2, and Vero E6-TMPRSS2-T2A-ACE2 cells were compared with those in HeLa229 cells (set to 1.0). *: p < 0.005, **: p < 0.0001. (B) Western blot analysis with an anti-human ACE2 antibody was performed to detect ACE2 protein in HeLa229, Vero E6, VeroE6/TMPRSS2, and Vero E6-TMPRSS2-T2A-ACE2 cells. beta -actin was used as a loading control. The amount of ACE2 protein detected in each cells was normalized with the that of beta -actin and expressed as a fold change (the amount in HeLa cells was set to 1.0). The transferred membrane was cut prior to hybridization with anti-ACE2 or anti-beta -actin. The original of blots with membrane edges visible was shown in the Supplementary figure S4. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39438626), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt, Ha

Species Glossary

Reactivity: Hu, Mu, Rt, Ha

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB933U-100UG
Alexa Fluor 405	FAB933V-100UG
Alexa Fluor 488	FAB933G-100UG
Alexa Fluor 532	AF933X-100UG
Alexa Fluor 594	FAB933T-100UG
Alexa Fluor 647	FAB933R-100UG
Alexa Fluor 700	FAB933N-100UG
Alexa Fluor 750	FAB933S-100UG
Alexa Fluor Plus 405	AF933AFP405-100UG
Alexa Fluor Plus 488	AF933AFP488-100UG
Alexa Fluor Plus 555	AF933AFP555-100UG
Alexa Fluor Plus 594	AF933AFP594-100UG
Alexa Fluor Plus 647	AF933AFP647-100UG
Alexa Fluor Plus 680	AF933AFP680-100UG
Allophycocyanin	FAB933A-100
Biotin	BAF933
CoraFluor 1	AF933CL1
DyLight 350	AF933D
DyLight 405	AF933E
DyLight 488	AF933K
DyLight 550	AF933L
DyLight 594	AF933M
DyLight 650	AF933W
DyLight 680	AF933Y
DyLight 755	AF933Z
FITC	AF933F
HRP	AF933H
Janelia Fluor 525	AF933JF525
Janelia Fluor 549	AF933I
Janelia Fluor 646	AF933J
Janelia Fluor 669	AF933JF669
PerCP	AF933C
Phycoerythrin	FAB933P-100
mFluor Violet 450 SE	AF933MFV450
mFluor Violet 500 SE	AF933MFV500
mFluor Violet 610 SE	AF933MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF933-SP
Carrier Free	FAB933V-100UG
Carrier Free	FAB933U-100UG
Carrier Free	FAB933T-100UG
Carrier Free	FAB933S-100UG
Carrier Free	FAB933R-100UG
Carrier Free	FAB933N-100UG
Carrier Free	FAB933G-100UG
Carrier Free	FAB933P-100
Carrier Free	FAB933A-100

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MAB933

ACE-2 Antibody (171606) [Unconjugated]

(60 Publications)

500 μg

MAB933

HAF007). A specific band was detected for ACE-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of NS0 mouse myeloma cell line and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB933) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for ACE-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NS0 mouse myeloma cell line and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB933) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ACE-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

ACE-2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB933) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to respiratory bronchioles. Staining was performed our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

ACE‑2 was detected in immersion fixed paraffin-embedded sections of hamster lung using Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB933) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to respiratory bronchioles. Staining was performed our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

In microvasculature, ACE2 is putatively expressed in pericytes. (A) Representative image of human pancreatic Formalin-Fixed Paraffin Embedded (FFPE) section stained for ACE2 in case #301118. In panel-a, a representative image of a pancreatic section showing two adjacent lobules (blue and red dotted lines) with different staining for ACE2 in endothelial cells/pericytes. A specific segmentation of the two lobules with high (blue) (zoom-in, panel-b) and low or null expression of ACE2 (red) (zoom-in, panel-c) is shown, suggesting lobularity of ACE2 expression in exocrine endothelial cells/pericytes of human pancreas. Scale bar in panel-a: 100 µm. Scale bar in panels-b and -c: 30 µm. (B) Double immunofluorescence staining of ACE2 (green) and CD31 (red) in FFPE pancreas sections from Body01A of Case #110118 (panels-a to -d) and of Body01B of Case #141117 (panels-e to -i). Digital zoom-in overlay images are shown in panels-d, -h and -i. Scale bar in panels-d and -g: 100 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33281748), licensed under a CC-BY license. Not internally tested by R&D Systems.

ACE2 staining pattern in human pancreas. Immunohistochemistry for ACE2 in human pancreatic tissue sections (case #110118) using R&D MAB933 antibody. ACE2 is markedly expressed in microvasculature associated cells (A, B) in some rare ductal cells (C, D) and in a subset of endocrine cells within pancreatic islets (E, F). Scale bars in (A, C, E) 150 µm. Scale bars in (B, D, F) 70 µm. Zoom-in images are reported in (B, D, F). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33281748), licensed under a CC-BY license. Not internally tested by R&D Systems.

Modulation of cell surface-expressed ATR1 and ACE2 molecules in Vero E6 cells infected with SARS-CoV-2 (24 h.p.i) and treated with Azilsartan (15 µM) for 72 hours by immunofluorescence microscopy. (A) Schematic flow of the analysis: Vero E6 cells were treated with various ARBs (the MTT assay previously defined non-cytotoxic concentrations: Azilosartan 15µM; Eprosartan 30µM; Irbesartan 60µM; Losartan 7µM; Olmesartan 15µM; Telmisartan 7µM; Valsartan 7µM) for 72 hours and were subsequently infected for analysis of ACE2 and ATR1 on treated and infected cells, 24 hours post-infection (h.p.i.). (B) The panel presents SARS-CoV-2 infected cells after incubation with Azilsartan (15 µM) and evaluation of fluorescence corresponding to the ATR1, ACE2, viral spike protein, and the nucleus of the cells. The merge of the images is displayed at the right of the panel. Images were acquired using a confocal microscope (Zeiss LSM 800) with a 63X/1.4 oil objective. (C) Quantitative representation of Mean Fluorescence corresponding to ATR1 and ACE2 molecules expression on VERO E6 cells treated or not treated with Azilsartan and SARS-CoV-2 in the cells. ***P < 0.001; ****P < 0.0001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34178717), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Ha

Species Glossary

Reactivity: Hu, Ha

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 532	FAB933X-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB933-SP

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(select up to 3 products from this page to compare)

AF3437

ACE-2 Antibody [Unconjugated]

(1 Review)

(20 Publications)

100 μg

AF3437

HAF019). A specific band was detected for ACE-2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for ACE-2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ACE-2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0108). Quadrant markers were set based on Goat IgG control antibody (AB-108-C, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox">

HEK293 human embryonic kidney cell line transfected with (A) mouse ACE-2 or (B) irrelevant protein, and eGFP was stained with Goat Anti-Mouse ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF3437) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (<a class=

F0108). Quadrant markers were set based on Goat IgG control antibody (AB-108-C, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

ACE‑2 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for <a class=

Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

ACE-2 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE-2 at approximately 141 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

MAB34371) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox">

Recombinant Mouse ACE‑2 protein was serially diluted 2-fold and captured by Rat Anti-Mouse ACE‑2 Monoclonal Antibody (<a class=

MAB34371) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

Expression of vasoactive proteins (mRNA and protein levels) in response to different oxygen conditions (mRNA: fold change relative to control condition: 21% O2; protein: % of control condition: 21% O2) as determined by qRT‐PCR using actb as house‐keeping gene and the delta delta Ct method and western blotting from cell lysates. (a) NOS3 mRNA expression, protein expression at 72 hr and Griess reaction (analyzed levels of NO2− and NO3− in the supernatants resulting from NOS activity). (b) ACE1, ACE2 mRNA expression and ACE1, ACE2 intracellular protein expression at 72 hr. (c) ACE1 and ACE2 protein levels in the cell culture supernatant as determined by Western blotting and mRNA expression of sheddases ADAM10 and ADAM17. p‐values result from statistical testing with Student's t‐test (mRNA), one‐way ANOVA (protein) and two‐way ANOVA (Griess) with Tukey's multiple comparison test. *p < .05; **p < .01; ***p < .001, ****p < .0001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33565273), licensed under a CC-BY license. Not internally tested by R&D Systems.

regDCs EXO are taken up by acceptor PBTECs, inhibiting ACE2 expression in vitro. (A) Uptake of Dil labeled EXO (red) by PBTECs, counterstained with nuclear stain DAPI (blue), phalloidin (Alex flour 647) for cell membrane and visualized under confocal microscopy. ACE2 mRNA expression (B) and flow cytometry scattergrams showing ACE2 positive cells percentage (C) in PBTECs treated or not treated with iDCs or regDCs EXO in the presence or absence of TGF beta R1 inhibitor SB431542. (D) representative bar graph of (C). Results shown are representative of three independent experiments (*P < 0.05 by one-way ANOVA followed by Tukey’s multiple comparisons). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33841418), licensed under a CC-BY license. Not internally tested by R&D Systems.

3437-ZN) was serially diluted and captured by Rat Anti-Mouse ACE‑2 Monoclonal Antibody (Catalog # MAB34371) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Mouse ACE‑2 (Catalog # <a class=

3437-ZN) was serially diluted and captured by Rat Anti-Mouse ACE‑2 Monoclonal Antibody (Catalog # MAB34371) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3437U-100UG
Alexa Fluor 405	AF3437V-100UG
Alexa Fluor 488	AF3437G-100UG
Alexa Fluor 532	AF3437X-100UG
Alexa Fluor 594	AF3437T-100UG
Alexa Fluor 647	AF3437R-100UG
Alexa Fluor 700	AF3437N-100UG
Alexa Fluor 750	AF3437S-100UG
Alexa Fluor Plus 405	AF3437AFP405-100UG
Alexa Fluor Plus 488	AF3437AFP488-100UG
Alexa Fluor Plus 555	AF3437AFP555-100UG
Alexa Fluor Plus 594	AF3437AFP594-100UG
Alexa Fluor Plus 647	AF3437AFP647-100UG
Alexa Fluor Plus 680	AF3437AFP680-100UG
Allophycocyanin	AF3437A
Biotin	AF3437B
CoraFluor 1	AF3437CL1
DyLight 350	AF3437D
DyLight 405	AF3437E
DyLight 488	AF3437K
DyLight 550	AF3437L
DyLight 594	AF3437M
DyLight 650	AF3437W
DyLight 680	AF3437Y
DyLight 755	AF3437Z
FITC	AF3437F
HRP	AF3437H
Janelia Fluor 525	AF3437JF525
Janelia Fluor 549	AF3437I
Janelia Fluor 646	AF3437J
Janelia Fluor 669	AF3437JF669
PE	AF3437P
PerCP	AF3437C
mFluor Violet 450 SE	AF3437MFV450
mFluor Violet 500 SE	AF3437MFV500
mFluor Violet 610 SE	AF3437MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3437-SP

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NBP2-67692

ACE-2 Antibody (SN0754)

(24 Publications)

100 ul

NBP2-67692

Immunocytochemistry/Immunofluorescence: ACE-2 Antibody (SN0754) [NBP2-67692] - Staining ACE2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ACE-2 Antibody (SN0754) [NBP2-67692] - ACE-2 staining in paraffin-embedded sections of hamster lung tissue using Rabbit Anti-Human ACE-2 (SN0754) Monoclonal Antibody (Catalog # NBP2-67692) at 3 ug/ml overnight at 4 degrees C. Detection kit: VC003. Counterstain: Hematoxylin. Localization: respiratory bronchioles.

Western Blot: ACE-2 Antibody (SN0754) [NBP2-67692] - Western blot analysis of ACE-2 on Hamster testis (1) and stomach (2) tissue lysates using anti-ACE-2 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: ACE-2 Antibody (SN0754) [NBP2-67692] - Staining ACE2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: ACE-2 Antibody (SN0754) [NBP2-67692] - Staining ACE2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ACE-2 Antibody (SN0754) [NBP2-67692] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-ACE2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ACE-2 Antibody (SN0754) [NBP2-67692] - Analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ACE-2 Antibody (SN0754) [NBP2-67692] - Analysis of paraffin-embedded mouse kidney tissue using anti-ACE2 antibody. Counter stained with hematoxylin.

Western blot analysis on different lysates with NBP2-67692 at 1/2,000 dilution. Lane 1: Caco-2 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Mouse testis tissue lysate (40 µg/Lane) Lane 5: Mouse lung tissue lysate (40 µg/Lane) Lane 6: Rat testis tissue lysate (40 µg/Lane) Lane 7: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 92 kDa Observed band size: 130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67692 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Reactivity:

Hu, Mu, Rt, Ha

Species Glossary

Reactivity: Hu, Mu, Rt, Ha

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP1-76614

ACE-2 Antibody - BSA Free

(8 Publications)

0.1 mg

NBP1-76614

Western Blot: ACE-2 Antibody [NBP1-76614] - Validation in Rat Skin Tissue Loading: 15 ug of lysates per lane.Antibodies: ACE2 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: ACE-2 Antibody [NBP1-76614] - Loading: 15 ug of lysates per lane.Antibodies: ACE2, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: ACE-2 Antibody [NBP1-76614] - Validation in Human TissuesLoading: 15 ug of lysates per lane.Antibodies: ACE2, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/Immunofluorescence: ACE-2 Antibody [NBP1-76614] - Analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 at 20 mg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing membrane staining on Caco2 cells.

Immunocytochemistry/Immunofluorescence: ACE-2 Antibody [NBP1-76614] - Analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 at 20 mg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76614] - Validation of ACE-2 in Rat Lung Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling ACE-2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76614] - Validation of ACE-2 in Human Testis Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling ACE-2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Reactivity:

Hu, Mu, Rt, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Bv

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76614AF350
Alexa Fluor 405	NBP1-76614AF405
Alexa Fluor 488	NBP1-76614AF488
Alexa Fluor 532	NBP1-76614AF532
Alexa Fluor 594	NBP1-76614AF594
Alexa Fluor 647	NBP1-76614AF647
Alexa Fluor 700	NBP1-76614AF700
Alexa Fluor 750	NBP1-76614AF750
Alexa Fluor Plus 405	NBP1-76614AFP405
Alexa Fluor Plus 488	NBP1-76614AFP488
Alexa Fluor Plus 555	NBP1-76614AFP555
Alexa Fluor Plus 594	NBP1-76614AFP594
Alexa Fluor Plus 647	NBP1-76614AFP647
Alexa Fluor Plus 680	NBP1-76614AFP680
Allophycocyanin	NBP1-76614APC
Biotin	NBP1-76614B
CoraFluor 1	NBP1-76614CL1
DyLight 350	NBP1-76614UV
DyLight 405	NBP1-76614V
DyLight 488	NBP1-76614G
DyLight 550	NBP1-76614R
DyLight 594	NBP1-76614DL594
DyLight 650	NBP1-76614C
DyLight 680	NBP1-76614FR
DyLight 755	NBP1-76614IR
FITC	NBP1-76614F
HRP	NBP1-76614H
Janelia Fluor 525	NBP1-76614JF525
Janelia Fluor 549	NBP1-76614JF549
Janelia Fluor 646	NBP1-76614JF646
Janelia Fluor 669	NBP1-76614JF669
PE	NBP1-76614PE
PerCP	NBP1-76614PCP
mFluor Violet 450 SE	NBP1-76614MFV450
mFluor Violet 500 SE	NBP1-76614MFV500
mFluor Violet 610 SE	NBP1-76614MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76614-0.025mg

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NBP2-80038

ACE-2 Antibody (AC384) - Azide and BSA Free

(7 Publications)

100 μg

NBP2-80038

Western Blot: ACE-2 Antibody (AC384) [NBP2-80038] - 1: Human ACE2 Fc-fusion protein. 2: Human ACE2 (Ecto domain) (FLAG®-tagged). 3: HepG2 cell lysate. 4: Other Fc-fusion (hGITR-Fc) proteins (negative control). 1:2'000 dilution.

Immunohistochemistry-Paraffin: ACE-2 Antibody (AC384) [NBP2-80038] - ACE-2 staining in paraffin-embedded sections of hamster lung tissue using Mouse Anti-Human ACE-2 (AC384) Monoclonal Antibody (Catalog # NBP2-80038) at 10 ug/ml overnight at 4 degrees C. Detection kit: VC001. Counterstain: Hematoxylin. Localization: respiratory bronchioles.

Flow Cytometry: ACE-2 Antibody (AC384) [NBP2-80038] - Detection of ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # NBP2-80038) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG1 control antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry-Paraffin: ACE-2 Antibody (AC384) [NBP2-80038] - ACE-2 was detected in immersion fixed paraffin-embedded sections of human lung normal (left) and lung cancer tissue (right) using Mouse Anti-HumanACE-2 (AC18F) Monoclonal Antibody (Catalog # NBP2-80038) at 15ug/mL for 1 hour at room temperature followed by incubation with the Goat anti-Mouse IgG Secondary Antibody.

Functional: ACE-2 Antibody (AC384) [NBP2-80038] - Binding of ACE2 (human) to the Spike protein of SARS-CoV-2 is inhibited by the antibody anti-ACE2 (human), mAb (blocking) (AC384). Spike (SARS-CoV-2):Fc (human) (RBD) (rec.) is coated on an ELISA plate at 1ug/ml. ACE2 (human), mAb (blocking) (AC384) or an unrelated mAb Control are added (starting at 40 ug/mL with a two-fold serial dilution) together with 500 ng/uL of ACE2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-80038AF350
Alexa Fluor 405	NBP2-80038AF405
Alexa Fluor 488	NBP2-80038AF488
Alexa Fluor 532	NBP2-80038AF532
Alexa Fluor 594	NBP2-80038AF594
Alexa Fluor 647	NBP2-80038AF647
Alexa Fluor 700	NBP2-80038AF700
Alexa Fluor 750	NBP2-80038AF750
Alexa Fluor Plus 405	NBP2-80038AFP405
Alexa Fluor Plus 488	NBP2-80038AFP488
Alexa Fluor Plus 555	NBP2-80038AFP555
Alexa Fluor Plus 594	NBP2-80038AFP594
Alexa Fluor Plus 647	NBP2-80038AFP647
Alexa Fluor Plus 680	NBP2-80038AFP680
Allophycocyanin	NBP2-80038APC
Biotin	NBP2-80038B
CoraFluor 1	NBP2-80038CL1
DyLight 350	NBP2-80038UV
DyLight 405	NBP2-80038V
DyLight 488	NBP2-80038G
DyLight 550	NBP2-80038R
DyLight 594	NBP2-80038DL594
DyLight 650	NBP2-80038C
DyLight 680	NBP2-80038FR
DyLight 755	NBP2-80038IR
FITC	NBP2-80038F
HRP	NBP2-80038H
Janelia Fluor 525	NBP2-80038JF525
Janelia Fluor 549	NBP2-80038JF549
Janelia Fluor 646	NBP2-80038JF646
Janelia Fluor 669	NBP2-80038JF669
PE	NBP2-80038PE
mFluor Violet 450 SE	NBP2-80038MFV450
mFluor Violet 500 SE	NBP2-80038MFV500
mFluor Violet 610 SE	NBP2-80038MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-80038-50ug

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MAB9332

ACE-2 Antibody (535919) [Unconjugated]

(7 Publications)

100 μg

MAB9332

HAF018). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human kidney and human testis. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Rat/Hamster ACE‑2 Monoclonal Antibody (Catalog # MAB9332) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

HAF018). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of human kidney and human testis. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Rat/Hamster ACE‑2 Monoclonal Antibody (Catalog # MAB9332) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox">

HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB9332) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

CTS013). Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007). Specific staining was localized to convoluted tubules. Staining was performed using our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

ACE‑2 was detected in immersion fixed frozen sections of rat kidney using Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB9332) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (<a class=

CTS013). Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007). Specific staining was localized to convoluted tubules. Staining was performed using our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to respiratory bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to respiratory bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Sialic acid has modest effect on Spike binding and viral entry. (B) Western blot for purified Fc-proteins from HEK293T probed with anti-Fc, anti-RBD or anti-ACE2 Ab. CD44-Fc is positive control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33103998), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Rt, Ha

Species Glossary

Reactivity: Hu, Rt, Ha

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB9332U-100UG
Alexa Fluor 405	FAB9332V-100UG
Alexa Fluor 488	FAB9332G-100UG
Alexa Fluor 532	FAB9332X
Alexa Fluor 594	FAB9332T-100UG
Alexa Fluor 647	FAB9332R-100UG
Alexa Fluor 700	FAB9332N-100UG
Alexa Fluor 750	FAB9332S-100UG
Alexa Fluor Plus 405	FAB9332AFP405-100UG
Alexa Fluor Plus 488	FAB9332AFP488-100UG
Alexa Fluor Plus 555	FAB9332AFP555-100UG
Alexa Fluor Plus 594	FAB9332AFP594-100UG
Alexa Fluor Plus 647	FAB9332AFP647-100UG
Alexa Fluor Plus 680	FAB9332AFP680-100UG
Allophycocyanin	FAB9332A
Allophycocyanin/Cy7	FAB9332APCCY7
Biotin	FAB9332B
CoraFluor 1	FAB9332CL1
DyLight 350	FAB9332D
DyLight 405	FAB9332E
DyLight 488	FAB9332K
DyLight 550	FAB9332L
DyLight 594	FAB9332M
DyLight 650	FAB9332W
DyLight 680	FAB9332Y
DyLight 755	FAB9332Z
FITC	FAB9332F
HRP	FAB9332H
Janelia Fluor 525	FAB9332JF525
Janelia Fluor 549	FAB9332I
Janelia Fluor 646	FAB9332J
Janelia Fluor 669	FAB9332JF669
PE/Atto594	FAB9332PEATT594
PE/Cy5.5	FAB9332PECY55
PE/Cy7	FAB9332PECY7
PerCP	FAB9332C
Phycoerythrin	FAB9332P-100
mFluor Violet 450 SE	FAB9332MFV450
mFluor Violet 500 SE	FAB9332MFV500
mFluor Violet 610 SE	FAB9332MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB9332S-100UG
Carrier Free	FAB9332R-100UG
Carrier Free	FAB9332N-100UG
Carrier Free	FAB9332G-100UG
Carrier Free	MAB9332-SP
Carrier Free	FAB9332V-100UG
Carrier Free	FAB9332U-100UG
Carrier Free	FAB9332T-100UG
Carrier Free	FAB9332P-100

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MAB9331

ACE-2 Antibody (171608) [Unconjugated]

(8 Publications)

100 μg

MAB9331

HAF007). A specific band was detected for ACE-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

HAF007). A specific band was detected for ACE-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NS0 mouse myeloma cell line and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB9331) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ACE-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS013). Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007). Specific staining was localized to convoluted tubules. Staining was performed using our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

ACE‑2 was detected in immersion fixed frozen sections of rat kidney using Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB9331) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (<a class=

CTS013). Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007). Specific staining was localized to convoluted tubules. Staining was performed using our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to respiratory bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to respiratory bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Reactivity:

Hu, Rt, Ha

Species Glossary

Reactivity: Hu, Rt, Ha

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB9331U-100UG
Alexa Fluor 405	FAB9331V-100UG
Alexa Fluor 488	FAB9331G-100UG
Alexa Fluor 532	FAB9331X-100UG
Alexa Fluor 594	FAB9331T-100UG
Alexa Fluor 647	FAB9331R-100UG
Alexa Fluor 700	FAB9331N-100UG
Alexa Fluor 750	FAB9331S-100UG
Alexa Fluor Plus 405	FAB9331AFP405-100UG
Alexa Fluor Plus 488	FAB9331AFP488-100UG
Alexa Fluor Plus 555	FAB9331AFP555-100UG
Alexa Fluor Plus 594	FAB9331AFP594-100UG
Alexa Fluor Plus 647	FAB9331AFP647-100UG
Alexa Fluor Plus 680	FAB9331AFP680-100UG
Allophycocyanin	FAB9331A
Biotin	FAB9331B
CoraFluor 1	FAB9331CL1
DyLight 350	FAB9331D
DyLight 405	FAB9331E
DyLight 488	FAB9331K
DyLight 550	FAB9331L
DyLight 594	FAB9331M
DyLight 650	FAB9331W
DyLight 680	FAB9331Y
DyLight 755	FAB9331Z
FITC	FAB9331F
HRP	FAB9331H
Janelia Fluor 525	FAB9331JF525
Janelia Fluor 549	FAB9331I
Janelia Fluor 646	FAB9331J
Janelia Fluor 669	FAB9331JF669
PE	FAB9331P
PerCP	FAB9331C
mFluor Violet 450 SE	FAB9331MFV450
mFluor Violet 500 SE	FAB9331MFV500
mFluor Violet 610 SE	FAB9331MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB9331-SP

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MAB3437

ACE-2 Antibody (460502) [Unconjugated]

(7 Publications)

100 μg

MAB3437

HAF005). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human kidney. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human/Mouse/Rat/Hamster ACE‑2 Monoclonal Antibody (Catalog # MAB3437) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (<a class=

HAF005). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of human kidney. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human/Mouse/Rat/Hamster ACE‑2 Monoclonal Antibody (Catalog # MAB3437) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF005). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western blot shows recombinant human ACE-2 protein, recombinant mouse ACE-2 protein, recombinant rat ACE-2 protein, and recombinant hamster ACE-2 protein. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human/Mouse/Rat/Hamster ACE‑2 Monoclonal Antibody (Catalog # MAB3437) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (<a class=

HAF005). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

Down-regulated ACE2 expression in mice challenged with cationic PAMAM dendrimers. (A) Levels of AngII in the plasma of the vehicle- (control) and nanoparticle-treated (15 μg/g) mice at 3 hrs after administration. AngII levels were determined using radioimmunoassays. n = 4–5 mice per group. *p < 0.05 or **p < 0.01 for the comparison of the G4, G5-, and G6-treated groups with the vehicle (control) group (two-tailed t-test). (B) The ACE2 mRNA relative expression level of the vehicle- (control) and nanoparticle-treated (15 μg/g) mice at 3 hrs after administration. Data were normalized to the expression of beta -actin reference gene. (two-tailed t-test). (C) Western blots of total lung samples obtained 3 hrs after the instillation of nanoparticles (15 μg/g). The blots are representative of three different mice for each treatment. Quantitative analyses of the ACE and ACE2 protein levels are illustrated. The levels are shown as the mean ACE- and ACE2-to-beta -actin ratios ± SEM. n = 3 mice per treatment. **p < 0.01 for the comparison of the G5-, and G6-treated groups with the vehicle group (two-tailed t-test). (D) Binding of G5 and G5.5 nanoparticles to recombinant human ACE2 at different concentrations was measured by surface plasmon resonance (SPR). The detailed dynamic binding constant and equilibrium dissociation constant are shown in Table S2. (E) G5 and G5.5 nanoparticles at different concentrations were incubated with recombinant ACE2 and AngII. The levels of AngII in the enzymatic activity measurement system were determined by radioimmunoassay. n = 3 tests per group. (two-tailed t-tests). Data are shown as the mean values ± SEM. *p < 0.05 or **p < 0.01; N.S. means not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25890286), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt, Ha

Species Glossary

Reactivity: Hu, Mu, Rt, Ha

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3437U-100UG
Alexa Fluor 405	FAB3437V-100UG
Alexa Fluor 488	FAB3437G-100UG
Alexa Fluor 532	FAB3437X-100UG
Alexa Fluor 594	FAB3437T-100UG
Alexa Fluor 647	FAB3437R-100UG
Alexa Fluor 700	FAB3437N-100UG
Alexa Fluor 750	FAB3437S-100UG
Alexa Fluor Plus 405	FAB3437AFP405-100UG
Alexa Fluor Plus 488	FAB3437AFP488-100UG
Alexa Fluor Plus 555	FAB3437AFP555-100UG
Alexa Fluor Plus 594	FAB3437AFP594-100UG
Alexa Fluor Plus 647	FAB3437AFP647-100UG
Alexa Fluor Plus 680	FAB3437AFP680-100UG
Allophycocyanin	FAB3437A
Biotin	FAB3437B
CoraFluor 1	FAB3437CL1
DyLight 350	FAB3437D
DyLight 405	FAB3437E
DyLight 488	FAB3437K
DyLight 550	FAB3437L
DyLight 594	FAB3437M
DyLight 650	FAB3437W
DyLight 680	FAB3437Y
DyLight 755	FAB3437Z
FITC	FAB3437F
HRP	FAB3437H
Janelia Fluor 525	FAB3437JF525
Janelia Fluor 549	FAB3437I
Janelia Fluor 646	FAB3437J
Janelia Fluor 669	FAB3437JF669
PE	FAB3437P
PerCP	FAB3437C
mFluor Violet 450 SE	FAB3437MFV450
mFluor Violet 500 SE	FAB3437MFV500
mFluor Violet 610 SE	FAB3437MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3437-SP

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NBP2-80035

ACE-2 Antibody (AC18F) - Azide and BSA Free

(1 Review)

(9 Publications)

100 μg

NBP2-80035

<b>Biological Strategies Validation. </b>Flow Cytometry: ACE-2 Antibody (AC18F) [NBP2-80035] - Detection of endogenous human ACE2 by anti-ACE2 (h), mAb (AC18F). HepG2 cell line treated with CellTase solution (Abeomics) before analysis and then stained with anti-ACE2 (h), mAb (AC18F) (red line) or an isotype control, mouse IgG1 (green line) at 1ug/10E6 cells each, revealed with a PE-conjugated goat anti-mouse IgG and then analyzed by flow cytometry.

Flow Cytometry: ACE-2 Antibody (AC18F) [NBP2-80035] - HepG2 cells were stained significantly using anti-ACE2 (human), mAb (AC18F).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-80035AF350
Alexa Fluor 405	NBP2-80035AF405
Alexa Fluor 488	NBP2-80035AF488
Alexa Fluor 532	NBP2-80035AF532
Alexa Fluor 594	NBP2-80035AF594
Alexa Fluor 647	NBP2-80035AF647
Alexa Fluor 700	NBP2-80035AF700
Alexa Fluor 750	NBP2-80035AF750
Alexa Fluor Plus 405	NBP2-80035AFP405
Alexa Fluor Plus 488	NBP2-80035AFP488
Alexa Fluor Plus 555	NBP2-80035AFP555
Alexa Fluor Plus 594	NBP2-80035AFP594
Alexa Fluor Plus 647	NBP2-80035AFP647
Alexa Fluor Plus 680	NBP2-80035AFP680
Allophycocyanin	NBP2-80035APC
Biotin	NBP2-80035B
CoraFluor 1	NBP2-80035CL1
DyLight 350	NBP2-80035UV
DyLight 405	NBP2-80035V
DyLight 488	NBP2-80035G
DyLight 550	NBP2-80035R
DyLight 594	NBP2-80035DL594
DyLight 650	NBP2-80035C
DyLight 680	NBP2-80035FR
DyLight 755	NBP2-80035IR
FITC	NBP2-80035F
HRP	NBP2-80035H
Janelia Fluor 525	NBP2-80035JF525
Janelia Fluor 549	NBP2-80035JF549
Janelia Fluor 646	NBP2-80035JF646
Janelia Fluor 669	NBP2-80035JF669
PE	NBP2-80035PE
PerCP	NBP2-80035PCP
mFluor Violet 450 SE	NBP2-80035MFV450
mFluor Violet 500 SE	NBP2-80035MFV500
mFluor Violet 610 SE	NBP2-80035MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-80035-50ug

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NBP1-76611

ACE-2 Antibody - BSA Free

0.1 mg

NBP1-76611

Western Blot: ACE-2 Antibody [NBP1-76611] - Loading: 15 ug of lysates per lane. Antibodies: ACE2, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution.

Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-76611] - Human kidney cells using Angiotensin Converting Enzyme 2 antibody at 2 ug/ml.

Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76611] - Validation of ACE-2 in Human Testis Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling ACE-2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76611] - Validation of ACE-2 In Caco2 Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE-2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing membrane staining on Caco2 cells.

Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76611] - Validation of ACE-2 in Human Lung Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76611] - Validation of ACE-2 in Rat Lung Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling ACE-2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76611] - Validation of ACE-2 in Mouse Lung Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling ACE-2 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunocytochemistry/ Immunofluorescence: ACE-2 Antibody - BSA Free [NBP1-76611] - Validation of ACE-2 in Human Kidney Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney cells labeling ACE-2 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5545

Alt Catalog# IMG-5545

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76611AF350
Alexa Fluor 405	NBP1-76611AF405
Alexa Fluor 488	NBP1-76611AF488
Alexa Fluor 532	NBP1-76611AF532
Alexa Fluor 594	NBP1-76611AF594
Alexa Fluor 647	NBP1-76611AF647
Alexa Fluor 700	NBP1-76611AF700
Alexa Fluor 750	NBP1-76611AF750
Alexa Fluor Plus 405	NBP1-76611AFP405
Alexa Fluor Plus 488	NBP1-76611AFP488
Alexa Fluor Plus 555	NBP1-76611AFP555
Alexa Fluor Plus 594	NBP1-76611AFP594
Alexa Fluor Plus 647	NBP1-76611AFP647
Alexa Fluor Plus 680	NBP1-76611AFP680
Allophycocyanin	NBP1-76611APC
Biotin	NBP1-76611B
CoraFluor 1	NBP1-76611CL1
DyLight 350	NBP1-76611UV
DyLight 405	NBP1-76611V
DyLight 488	NBP1-76611G
DyLight 550	NBP1-76611R
DyLight 594	NBP1-76611DL594
DyLight 650	NBP1-76611C
DyLight 680	NBP1-76611FR
DyLight 755	NBP1-76611IR
FITC	NBP1-76611F
HRP	NBP1-76611H
Janelia Fluor 525	NBP1-76611JF525
Janelia Fluor 549	NBP1-76611JF549
Janelia Fluor 646	NBP1-76611JF646
Janelia Fluor 669	NBP1-76611JF669
PE	NBP1-76611PE
PerCP	NBP1-76611PCP
mFluor Violet 450 SE	NBP1-76611MFV450
mFluor Violet 500 SE	NBP1-76611MFV500
mFluor Violet 610 SE	NBP1-76611MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76611-0.025mg

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NBP1-88123

ACE-2 Antibody - BSA Free

(2 Publications)

0.1 ml

NBP1-88123

Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-88123] - Staining of human duodenum shows strong positivity in apical membranes in glandular cells.

Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-88123] - Staining of human kidney shows strong positivity in apical membrane in cells in tubules.

Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-88123] - Staining of human tonsil shows no positivity in lymphoid cells as expected.

Flow Cytometry: ACE-2 Antibody [NBP1-88123] - Detection of ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Rabbit Anti-Human ACE-2 Polyclonal Antibody (Catalog # NBP1-88123) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on Rabbit IgG control antibody staining (Catalog # MAB1050, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

ACE-2 Antibodies

ACE-2 Antibody [Unconjugated]

ACE-2 Antibody (171606) [Unconjugated]

ACE-2 Antibody [Unconjugated]

ACE-2 Antibody (SN0754)

ACE-2 Antibody - BSA Free

ACE-2 Antibody (AC384) - Azide and BSA Free

ACE-2 Antibody (535919) [Unconjugated]

ACE-2 Antibody (171608) [Unconjugated]

ACE-2 Antibody (460502) [Unconjugated]

ACE-2 Antibody (AC18F) - Azide and BSA Free

ACE-2 Antibody - BSA Free

ACE-2 Antibody - BSA Free