Acetylcholinesterase/ACHE Antibody

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Western Blot: Acetylcholinesterase/ACHE Antibody [NB100-1519] - 0.3ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Acetylcholinesterase/ACHE Antibody Summary

Immunogen
Peptide with sequence QFDHYSKQDRCSDL corresponding to C-Terminus according to NP_000656.1.
Marker
Early Neuronal Development Marker
Specificity
This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACHE
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 67 kDa band observed in human brain (cerebellum and hippocampus) lysates (calculated MW of 67 kDa band according to NP_000656.1).
Positive Control
Brain Lysate (NB820-59177)
Acetylcholinesterase/ACHE Lysate (NBL1-07238)
Reviewed Applications
Read 1 Review rated 5
using
NB100-1519 in the following applications:

Publications
Read Publication using NB100-1519.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Bovine.his batch is not expected to cross-react with Mouse and Rat in Western blot.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Acetylcholinesterase/ACHE Antibody

  • acetylcholinesterase (Yt blood group)
  • Acetylcholinesterase
  • ACHE
  • apoptosis-related acetylcholinesterase
  • ARACHE
  • EC 3.1.1
  • EC 3.1.1.7
  • N-ACHE
  • Yt blood group
  • YT

Background

Acetylcholinesterase, or ACHE, hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Acetylcholinesterase/ACHE Antibody (NB100-1519)(1)

Review for Acetylcholinesterase/ACHE Antibody (NB100-1519) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-1519:
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 Acetylcholinesterase/ACHE NB100-1519
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reviewed by:
Anonymous
Mouse 11/21/2016
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Summary

Sample Tested Mouse brain
SpeciesMouse

Comments

CommentsFloating sections were incubated overnight at 4C with a 1:200 dilution of the above ACHE antibody, washed and then incubated for 1 hour at room temperature with a 1:500 dilution of donkey anti-rabbit DyLight 594 secondary antibody (Cat#NBP1-75637).

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Anonymous
11/21/2016
Application:
Species: Mouse

Bioinformatics

Gene Symbol ACHE
Entrez
Uniprot