Acetylcholinesterase/ACHE Antibody

Images

 
Western Blot: Acetylcholinesterase/ACHE Antibody [NB100-1519] - (0.3ug/ml) staining of Daudi (A), HeLa (B) and Jurkat (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NB100-1519] - analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by ...read more
Flow Cytometry: Acetylcholinesterase/ACHE Antibody [NB100-1519] - analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Western Blot: Acetylcholinesterase/ACHE Antibody [NB100-1519] - (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Acetylcholinesterase/ACHE Antibody Summary

Immunogen
Peptide with sequence QFDHYSKQDRCSDL corresponding to C-Terminus according to NP_000656.1.
Marker
Early Neuronal Development Marker
Specificity
This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACHE
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1-0.5 ug/ml
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
Western blot: Approx 65-70kDa band observed in lysates of cell lines Daudi, HeLa and Jurkat, and approx. 70kDa in Rat Brain lysates (calculated MW of 67.8kDa according to Human NP_000656.1 and 68.2kDa according to Rat NP_742006.1).Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nucleus of U2OS cells.
Flow Cytometry: Flow cytometric analysis of HeLa cells.
Control
Brain Lysate (NB820-59177)
Acetylcholinesterase/ACHE Lysate (NBL1-07238)
Acetylcholinesterase/ACHE Lysate (NBP2-66223)
Reviewed Applications
Read 1 Review rated 5
using
NB100-1519 in the following applications:

Publications
Read Publication using NB100-1519.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Acetylcholinesterase/ACHE Antibody

  • acetylcholinesterase (Yt blood group)
  • Acetylcholinesterase
  • ACHE
  • apoptosis-related acetylcholinesterase
  • ARACHE
  • EC 3.1.1
  • EC 3.1.1.7
  • N-ACHE
  • Yt blood group
  • YT

Background

Acetylcholinesterase, or ACHE, hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-03523
Species: Hu, Ca
Applications: WB, Flow
NBP1-30052
Species: Hu, Mu, Rt, Av, Ch, GP, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP2-13306
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-86614
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-55298
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-13075
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
NB300-109
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, ICC, IHC-FrFl
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB300-187
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC
NBP1-89727
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-49118
Species: Hu
Applications: IHC, IHC-P
NBP1-85740
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
H00010908-M08
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP1-05163
Species: Hu, Rt, Po, Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
7815-NG/CF
Species: Rt

Publications for Acetylcholinesterase/ACHE Antibody (NB100-1519)(1)

Review for Acetylcholinesterase/ACHE Antibody (NB100-1519) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-1519:
Filter by Applications
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 Acetylcholinesterase/ACHE NB100-1519
Enlarge
reviewed by:
Anonymous
Mouse 11/21/2016
View

Summary

Sample Tested Mouse brain
SpeciesMouse

Comments

CommentsFloating sections were incubated overnight at 4C with a 1:200 dilution of the above ACHE antibody, washed and then incubated for 1 hour at room temperature with a 1:500 dilution of donkey anti-rabbit DyLight 594 secondary antibody (Cat#NBP1-75637).

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Acetylcholinesterase/ACHE Antibody (NB100-1519) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Acetylcholinesterase/ACHE Antibody (NB100-1519)

Discover related pathways, diseases and genes to Acetylcholinesterase/ACHE Antibody (NB100-1519). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Acetylcholinesterase/ACHE Antibody (NB100-1519)

Discover more about diseases related to Acetylcholinesterase/ACHE Antibody (NB100-1519).
 

Pathways for Acetylcholinesterase/ACHE Antibody (NB100-1519)

View related products by pathway.

PTMs for Acetylcholinesterase/ACHE Antibody (NB100-1519)

Learn more about PTMs related to Acetylcholinesterase/ACHE Antibody (NB100-1519).
 

Research Areas for Acetylcholinesterase/ACHE Antibody (NB100-1519)

Find related products by research area.

Blogs on Acetylcholinesterase/ACHE

There are no specific blogs for Acetylcholinesterase/ACHE, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
11/21/2016
Application:
Species: Mouse

Bioinformatics

Gene Symbol ACHE
Entrez
Uniprot