Western Blot: Acetylcholinesterase/ACHE Antibody [NB100-1519] - (0.3ug/ml) staining of Daudi (A), HeLa (B) and Jurkat (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NB100-1519] - analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by ...read more
Flow Cytometry: Acetylcholinesterase/ACHE Antibody [NB100-1519] - analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Western Blot: Acetylcholinesterase/ACHE Antibody [NB100-1519] - (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Western blot: Approx 65-70kDa band observed in lysates of cell lines Daudi, HeLa and Jurkat, and approx. 70kDa in Rat Brain lysates (calculated MW of 67.8kDa according to Human NP_000656.1 and 68.2kDa according to Rat NP_742006.1).Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse Brain for this particular batch. Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nucleus of U2OS cells. Flow Cytometry: Flow cytometric analysis of HeLa cells.
Predicted cross-reactivity based on sequence identity: Mouse
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for Acetylcholinesterase/ACHE Antibody
acetylcholinesterase (Yt blood group)
Yt blood group
Acetylcholinesterase, or ACHE, hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Floating sections were incubated overnight at 4C with a 1:200 dilution of the above ACHE antibody, washed and then incubated for 1 hour at room temperature with a 1:500 dilution of donkey anti-rabbit DyLight 594 secondary antibody (Cat#NBP1-75637).
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