Collectrin/TMEM27 Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Collectrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4965) followed by HRP-conjugated ...read more
Collectrin was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Collectrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4965) at 1.7 µg/mL overnight at 4 °C. Tissue was ...read more
Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Collectrin at approximately 69 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse Collectrin ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Collectrin/TMEM27 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Collectrin
Glu15-Ile144
Accession # Q9ESG4
Specificity
Detects mouse Collectrin in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Collectrin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
TMEM27
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publication using
AF4965 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Collectrin/TMEM27 Antibody [Unconjugated]

  • Collectrin
  • kidney-specific membrane protein
  • NX17
  • NX-17
  • TMEM27
  • transmembrane protein 27NX170610008J07Rik

Background

Collectrin (also Transmembrane protein 27) is a 37‑43 kDa member of the MEM27 family of proteins. It is expressed on pancreatic beta -cells where it regulates insulin secretion, and on renal tubular epithelium where it mediates amino acid transport. Mature mouse Collectrin is a 208 amino acid (aa) type I transmembrane glycoptotein. It contains a 127 aa extracellular region (aa 15‑141) and a 60 aa cytoplasmic domain (aa 163‑222). Collectrin dimerizes on the cell surface, and undergoes proteolytic cleavage to generate a 26 kDa soluble fragment. Over aa 15‑144, mouse Collectrin shares 92% and 86% aa identity with rat and human Collectrin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Collectrin/TMEM27 Antibody (AF4965)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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(1)
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF4965

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Bioinformatics

Gene Symbol TMEM27
Uniprot