53BP1 Antibody


Western Blot: 53BP1 Antibody [NB100-904] - Analysis in untreated U2OS cell lysate.
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-904] - Analysis in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).
Immunohistochemistry: 53BP1 Antibody [NB100-904] - Analysis in human renal cancer using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-904] - Analysis in HeLa cells. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-904] - Analysis in MEFs, HeLa, C33A and CaSki cells. Image courtesy of an anonymous customer review.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, PLA
1 mg/ml

53BP1 Antibody Summary

A synthetic peptide corresponding to a portion of human 53BP1 encoded in exon 11, 12 and 19 [UniProt# Q12888]. This antibody is a cocktail hybrid of NB100-304 and NB100-305.
DNA Double Strand Break Marker
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at -20 °C.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.1% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Immunoprecipitation
  • Proximity Ligation Assay
Application Notes
This 53BP1 antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot and Immunohistochemistry paraffin embedded sections. In Western blot a band was observed at ~220 kD. Irradiated lysates demonstrate a gel shift. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in immunprecipitation reported in scientific literature (PMID 25512560). Use in proximity ligation assay reported in scientific literature (PMID 26734725)
Reviewed Applications
Read 3 Reviews rated 5
NB100-904 in the following applications:

Read Publications using
NB100-904 in the following applications:

  • 28 publications
  • IP
    1 publication
  • PLA
    1 publication
  • WB
    10 publications

Reactivity Notes

Human has been tested in WB, IHC and ICC/IF, mouse has only been tested in ICC/IF.

Alternate Names for 53BP1 Antibody

  • 53BP1
  • MGC138366
  • p202
  • p53-binding protein 1
  • p53BP1,53BP1FLJ41424
  • TP53BP1
  • tumor protein 53-binding protein, 1
  • tumor protein p53 binding protein 1
  • tumor protein p53-binding protein, 1
  • tumor suppressor p53-binding protein 1


53BP1 (p53 binding protein 1) plays a key role in response to DNA damage, checkpoint signaling during mitosis and enhancing TP53-mediated transcriptional activation. Originally identified as p53's transcriptional enhancing partner, 53BP1 now has been established as a substrate for ATM (ataxia telangiectasia mutated) signaling and that it relocalizes to discrete foci overlapping with gamma H2AX (phosphorylated histone H2AX); demarcating DNA double strand breaks (DSBs) sites following exposure to radiation. 53BP1 functions downstream of gamma H2AX-dependent hierarchy of proteins that collectively establish IRIF (ionizing radiation induced foci) at DSBs; this hierarchy includes Mre11/Rad50/NBS1 (MRN complex), ATM, MDC1, RNF8, RNF168 and HERC2. With the exception of ATM, whose function to generate gamma H2AX may be partially compensated by the activity of DNA-PK (DNA-dependent kinase), all of these proteins are physically and functionally required to recruit 53BP1 to the DSB site. Briefly, this process involves DSB recognition by MRN, ATM activation, gamma H2AX-formation, MDC1-recruitment, MRN-retention (leading to further ATM-activation and gamma H2AX spreading) and RNF8/RNF168/HERC2-mediated histone H2A and H2AX mono and poly-ubiquitination.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Ca
Applications: IP (-), WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB
Species: Hu, Mu
Applications: IP (-), WB, ICC/IF, PLA
Species: Hu, Mu
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC
Species: Hu, Mu, Ha, Ma, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IP
Species: Hu
Applications: WB, Func, ICC/IF, IP
Species: Hu, Mu, Rt, Ch, Ha
Applications: WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: WB, Flow, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Simple Western, IHC
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, PLA
Species: Hu, Mu, Rt, Pm
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Ce, Ch
Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: WB, IHC, IHC-P, IP
Species: Hu
Applications: WB
Species: Mu
Applications: WB
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP, PLA

Publications for 53BP1 Antibody (NB100-904)(57)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IP, PLA, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 57. Show All 57 Publications.
Publications using NB100-904 Applications Species
Saredi G, Huang H, Hammond CM et al. H4K20me0 marks post-replicative chromatin and recruits the TONSL-MMS22L DNA repair complex. Nature Jun 22 2016 12:00AM [PMID:27338793] (WB) WB
Pederiva C, Bohm S, Julner A, Farnebo M. Splicing controls the ubiquitin response during DNA double-strand break repair. Cell Death Differ. Jun 17 2016 12:00AM [PMID:27315300] (ICC/IF, WB) ICC/IF, WB
Rassoolzadeh H, Bohm S, Hedstrom E et al. Overexpression of the scaffold WD40 protein WRAP53b enhances the repair of and cell survival from DNA double-strand breaks. Cell Death Dis Jun 17 2016 12:00AM [PMID:27310875] (ICC/IF, WB) ICC/IF, WB
Avila AI, Illing A, Becker F et al. Xpg limits the expansion of haematopoietic stem and progenitor cells after ionising radiation. Nucleic Acids Res. May 2 2016 12:00AM [PMID:27137888] (ICC/IF, Mouse) ICC/IF Mouse
Mackenzie KJ, Carroll P, Lettice L et al. Ribonuclease H2 mutations induce a cGAS/STING-dependent innate immune response. EMBO J. 2016 Feb 22 [PMID:26903602] (ICC/IF, Mouse) ICC/IF Mouse
Rassoolzadeh H, Coucoravas C, Farnebo M. The proximity ligation assay reveals that at DNA double-strand breaks WRAP53B associates with gammaH2AX and controls interactions between RNF8 and MDC1. Nucleus. 2015 Sep 03 [PMID:26734725] (PLA, Human) PLA Human
Park J, Welner RS, Chan MY et al. The DNA Ligase IV Syndrome R278H Mutation Impairs B Lymphopoiesis via Error-Prone Nonhomologous End-Joining. J. Immunol. 2016 Jan 01 [PMID:26608917] (ICC/IF) ICC/IF
Hausmann C, Temme A, Cordes N, Eke I. ILKAP, ILK and PINCH1 control cell survival of p53-wildtype glioblastoma cells after irradiation. Oncotarget 2015 Oct 27 [PMID:26460618]
Hedstrom E, Pederiva C, Farnebo J et al. Downregulation of the cancer susceptibility protein WRAP53beta in epithelial ovarian cancer leads to defective DNA repair and poor clinical outcome. Cell Death Dis 2015 [PMID:26426684] (ICC/IF) ICC/IF
Boysen G, Barbieri CE, Prandi D et al. SPOP mutation leads to genomic instability in prostate cancer. Elife 2015 [PMID:26374986] (ICC/IF) ICC/IF
Show All 57 Publications.

Reviews for 53BP1 Antibody (NB100-904) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.
We have 3 review tested in 1 application: IF.

Reviews using NB100-904:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
 53BP1 NB100-904
reviewed by:
Human 11/20/2014


Sample TestedHeLa cells


Blocking Details2% BSA in PBS, 1 h at RT

Primary Anitbody

Dilution Ratio1:2000 in 2% BSA/PBS, 14h at 4C

Secondary Antibody

Secondary DescriptionAlexa Fluor® 488 Goat Anti-Mouse IgG (H+L)
Secondary Manufacturer Cat#A-11001 from Life Technologies (by Molecular Probes)
Secondary Concentration0.2 ug/ml


Detection NotesZeiss LSM 780 confocal microscope, x63 oil objective
Fixation Details4% Paraformaldehyde as fixative, permeabilization with 0.2% Triton-X100
Wash Description3x washes in PBS for 5 min
reviewed by:
IF Human 08/24/2012


Sample TestedHuman tumor cell lines: A549, FaDu, MiaPaCa2, UTSCC15


Blocking Details1% BSA, 30 min, RT

Primary Anitbody

Dilution Ratio1:2000 1h RT

Secondary Antibody

Secondary DescriptionAlexaFluor488 (anti-Rb)
Secondary Manufacturer Cat#Invitrogen A11034
Secondary Concentration1:2000


Detection Notesimages with LSM
Fixation DetailsFixation: 3%FA, Permeabilisation: Triton-X, Buffer: 1%BSA, 1h incubation RT
Wash Description1xPBS, 6x
Immunofluorescence 53BP1 NB100-904
reviewed by:
IF Mouse 05/22/2012


Sample TestedMEFs, HeLa, C33A, CaSki
CommentsComplete protocol can be found at: delangelab.rockefeller.edu/protocols_files/IF_protocol.pdf


Blocking DetailsPBG blocking 30mins @RT

Primary Anitbody

Dilution Ratio1:500 2h @RT

Secondary Antibody

Secondary DescriptionFITC conjugated Donkey anti-rabbit IgG
Secondary Manufacturer Cat#711-095-152
Secondary Concentration1:250


Detection NotesZeiss Observer A1, Axiovision
Fixation Details4% PFH fixation 10mins @RT, Triton-X permeabilization 10mins @RT, PBS wash 2x5mins @RT
Wash DescriptionPBG wash 3x5mins @RT


CommentsComplete protocol can be found at: delangelab.rockefeller.edu/protocols_files/IF_protocol.pdf

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 53BP1 Antibody (NB100-904). (Showing 1 - 5 of 5 FAQs).

  1. We're looking for anti53BP1 antibody covalently labeled with a fluorofor preferabley Cy3. Do you make it?
    • We sell 53BP1 antibodies available conjugated to DyLight 488, 550 (very similar in spectrum to Cy3) and 650. You may also purchase the unlabeled antibodies and conjugate them to the fluorophores of your choice. The catalog numbers that you may be interested in are: NB100-904R, NB100-304R, NB100-305R.
  2. Hello I want to ask you for an advice in case of antibodies. In our research we focus on DNA repair foci and use your Novus antibodies for phosphorylated histone H2AX (monoclonal mouse) and protein 53BP1(polyclonal rabbit). Now we want to use antobody for phosphorylated 53BP1 protein to increase specifity and I want to ask you which from your NOVUS antibodies will be the best for us.
    • We currently stock just one antibody to phosphorylated 53BP1, which you can see at the following link: NB100-1803. This is guaranteed for detection of 53BP1 (pSer25) in human and mouse samples by WB, FLOW, ICC/IF,IHC-P, IP and PLA. As this antibody is a rabbit polyclonal, you would be unable to stain for both 53BP1 and its phosphorylated isoform in the same sample, unless you used an antibody from a different host for detection of total 53BP1 or used directly conjugated primaries. Our mouse monoclonal to 53BP1, with catalogue number NBP2-25028, is validated for detection of the human protein by WB, FLOW and IHC. Unfortunately we do not currently stock any other antibodies to phosphorylated 53BP1.
  3. I would like to know if these antibodies are suitable with IHC techniques using HRP conjugated antibodies and DAB as chromogen? Cat# NB200-175, NB100-904 and NB100-142
    • Yes, all of these antibodies are useful for IHC-P with DAB based detection. Please note that the PTEN Antibody (28H6) NB200-175 has been discontinued, however.
  4. I would like to know if this ab would also work for phosphorylated 53BP1? Or if you have another ab that would detect it?
    • NB100-904 is a cocktail of NB100-304 and NB100-305. These two antibodies recognize between residues 350 and 400 and residues 1925 and the C-terminus (residue 1972), respectively. There are multiple phosphorylations between residues 350 and 400 and as you can see from our datasheet, NB100-304 recognizes 53BP1. We do sell one phosphospecific antibody to 53BP1, catalog number NB100-1803.
  5. Is there a recommended IHC-P protocol for the 53BP1 Antibody (NB100-904)?
    • We do not have a specific protocol outside of our general protocol for IHC for NB100-904. However, I would ensure that the protocol used includes ample permeabilization for a nuclear signal (usually 0.1-0.5% Triton X in the blocking and diluent buffers or a separate permeabilization step) and heat induced antigen retrieval with pH 6.0 citrate buffer. Our general protocols for both IHC-P and antigen retrieval are linked below. IHC-P Protocol Antigen Retrieival Protocol

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-904AF405
Alexa Fluor (R) 488 Labeled NB100-904AF488
Alexa Fluor (R) 647 Labeled NB100-904AF647
Alexa Fluor (R) 700 Labeled NB100-904AF700
Biotin Labeled NB100-904B
DyLight 405 Labeled NB100-904V
DyLight 488 Labeled NB100-904G
DyLight 550 Labeled NB100-904R
DyLight 650 Labeled NB100-904C
FITC Labeled NB100-904F
HRP Labeled NB100-904H

Additional 53BP1 Antibody Products

Related Products by Gene

Bioinformatics Tool for 53BP1 Antibody (NB100-904)

Discover related pathways, diseases and genes to 53BP1 Antibody (NB100-904). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for 53BP1 Antibody (NB100-904)

Discover more about diseases related to 53BP1 Antibody (NB100-904).

Pathways for 53BP1 Antibody (NB100-904)

View related products by pathway.

PTMs for 53BP1 Antibody (NB100-904)

Learn more about PTMs related to 53BP1 Antibody (NB100-904).

Research Areas for 53BP1 Antibody (NB100-904)

Find related products by research area.

Blogs on 53BP1.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

53BP1, DNA Damage Response and Tumor Suppression
53BP1 (p53 binding protein 1) was originally thought to be a p53 transcriptional enhancing partner, but now has been shown to be an ataxia telangiectasia mutated (ATM) substrate. It is a late DNA damage response (DDR) marker, appearing in the telophas...  Read full blog post.

53BP1, DNA Damage Response and Tumor Suppression
53BP1 (p53 binding protein 1) was originally thought to be a p53 transcriptional enhancing partner, but now has been shown to be an ataxia telangiectasia mutated (ATM) substrate. It is a late DNA damage response (DDR) marker, appearing in the telophas...  Read full blog post.

NUP153 & 53BP1: A Novel DNA Repair Pathway
Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De...  Read full blog post.

Blocking 53BP1 Expression Lessens Tumor Development in BRCA1-Defective Mice
Our antibody database at Novus Biologicals provides research tools for the forefront of cancer research. Recently, a mouse study using 53BP1 and BRCA1 antibodies showed that deletion of 53BP1 greatly lessened the incidence of tumor development in mice...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol TP53BP1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB100-904 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought