Histone H2AX [p Ser139] Antibody [Unconjugated]

Images

 
Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 μM camptothecin (CPT) for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 24 hours, loaded at 0.2 mg/mL. A specific band was detected for ...read more
Histone H2AX phosphorylated at S139 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with ultraviolet radiation using Rabbit Anti-Human Phospho-Histone H2AX (S139) Antigen ...read more
Histone H2AX was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human Phospho-Histone H2AX (S139) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2288) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Histone H2AX [p Ser139] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide containing human Histone H2AX S139 site
Modification
p Ser139
Specificity
Detects human Histone H2AX when phosphorylated at S139.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H2AFX
Purity
Antigen and protein A Affinity-purified
Purity Statement
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF2288 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen and protein A Affinity-purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Histone H2AX [p Ser139] Antibody [Unconjugated]

  • H2A.X
  • H2A/X
  • H2AFX
  • Histone H2AX

Background

Histone H2AX is phosphorylated at S139 in cells exposed to DNA double-strand break-inducing agents, such as ionizing radiation. The S139 phosphorylated H2AX, termed ( gamma -H2AX, marks the site of DNA double-strand breaks and serves to recruit cell cycle checkpoint and DNA repair factors to the site of damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H2AX Antibody (AF2288)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Goat.

We have publications tested in 3 applications: ICC, IHC, Western Blot.


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ICC
(8)
IHC
(2)
Western Blot
(5)
All Applications
Filter By Species
Human
(10)
Mouse
(4)
Goat
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using AF2288 Applications Species
DM Zhang, R Navara, T Yin, J Szymanski, U Goldsztejn, C Kenkel, A Lang, C Mpoy, CE Lipovsky, Y Qiao, S Hicks, G Li, KMS Moore, C Bergom, BE Rogers, CG Robinson, PS Cuculich, JK Schwarz, SL Rentschler Cardiac radiotherapy induces electrical conduction reprogramming in the absence of transmural fibrosis Nature Communications, 2021;12(1):5558. 2021 [PMID: 34561429] (IHC, Mouse) IHC Mouse
M Al-Mugotir, JJ Lovelace, J George, M Bessho, D Pal, L Struble, C Kolar, S Rana, A Natarajan, T Bessho, GEO Borgstahl Selective killing of homologous recombination-deficient cancer cell lines by inhibitors of the RPA:RAD52 protein-protein interaction PLoS ONE, 2021;16(3):e0248941. 2021 [PMID: 33784323] (Western Blot, Human) Western Blot Human
A Schepsky, G Asta Traus, J Petur Joel, S Ingthorsso, J Kricker, J Thor Bergt, A Asbjarnars, T Gudjonsson, N Nupponen, A Slipicevic, F Lehmann, T Gudjonsson Melflufen, a peptide-conjugated alkylator, is an efficient anti-neo-plastic drug in breast cancer cell lines Cancer Med, 2020;0(0):. 2020 [PMID: 32717133] (ICC, Human) ICC Human
CE Hellweg, V Shinde, SP Srinivasan, M Henry, T Rotshteyn, C Baumstark-, C Schmitz, S Feles, LF Spitta, R Hemmersbac, J Hescheler, A Sachinidis Radiation Response of Murine Embryonic Stem Cells Cells, 2020;9(7):. 2020 [PMID: 32660081] (ICC, Mouse) ICC Mouse
S Miranda, M Correia, AG Dias, A Pestana, P Soares, J Nunes, J Lima, V Máximo, P Boaventura Evaluation of the role of mitochondria in the non-targeted effects of ionizing radiation using cybrid cellular models Sci Rep, 2020;10(1):6131. 2020 [PMID: 32273537] (ICC, Human) ICC Human
HL Li, CY Wang, J Fu, XJ Yang, Y Sun, YH Shao, LH Zhang, XM Yang, XL Zhang, J Lin PTEN expression in U251 glioma cells enhances their sensitivity to ionizing radiation by suppressing DNA repair capacity Eur Rev Med Pharmacol Sci, 2019;23(23):10453-10458. 2019 [PMID: 31841199] (ICC, Human) ICC Human
Y Wang, AJ Bernhardy, J Nacson, JJ Krais, YF Tan, E Nicolas, MR Radke, E Handorf, A Llop-Gueva, J Balmaña, EM Swisher, V Serra, S Peri, N Johnson BRCA1 intronic Alu elements drive gene rearrangements and PARP inhibitor resistance Nat Commun, 2019;10(1):5661. 2019 [PMID: 31827092] (ICC, Human) ICC Human
Z Simandi, K Pajer, K Karolyi, T Sieler, LL Jiang, Z Kolostyak, Z Sari, Z Fekecs, A Pap, A Patsalos, GA Contreras, B Reho, Z Papp, X Guo, A Horvath, G Kiss, Z Keresztess, G Vámosi, J Hickman, H Xu, D Dormann, T Hortobagyi, M Antal, A Nogradi, L Nagy Arginine methyltransferase PRMT8 provides cellular stress tolerance in aging motoneurons J. Neurosci., 2018;0(0):. 2018 [PMID: 30054395] (Western Blot, Mouse) Western Blot Mouse
M Jangi, C Fleet, P Cullen, SV Gupta, S Mekhoubad, E Chiao, N Allaire, CF Bennett, F Rigo, AR Krainer, JA Hurt, JP Carulli, JF Staropoli SMN deficiency in severe models of spinal muscular atrophy causes widespread intron retention and DNA damage Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28270613] (IHC, Mouse) IHC Mouse
N Rebolleda, I Losada-Fer, G Perez-Chac, R Castejon, S Rosado, M Morado, MT Vallejo-Cr, A Martinez, JA Vargas-Nuñ, P Perez-Acie Synergistic Activity of Deguelin and Fludarabine in Cells from Chronic Lymphocytic Leukemia Patients and in the New Zealand Black Murine Model PLoS ONE, 2016;11(4):e0154159. 2016 [PMID: 27101369] (Western Blot, Human) Western Blot Human
Show All 16 Publications.

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Bioinformatics

Gene Symbol H2AFX