Histone H2AX [p Ser139] Antibody [Unconjugated]


Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 μM camptothecin (CPT) for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 24 hours, loaded at 0.2 mg/mL. A specific band was detected for ...read more
Histone H2AX phosphorylated at S139 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with ultraviolet radiation using Rabbit Anti-Human Phospho-Histone H2AX (S139) Antigen ...read more
Histone H2AX was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human Phospho-Histone H2AX (S139) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2288) ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Histone H2AX [p Ser139] Antibody [Unconjugated] Summary

Phosphopeptide containing human Histone H2AX S139 site
p Ser139
Detects human Histone H2AX when phosphorylated at S139.
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.5 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read Publications using
AF2288 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Antigen and protein A Affinity-purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Histone H2AX [p Ser139] Antibody [Unconjugated]

  • H2AFX
  • Histone H2AX


Histone H2AX is phosphorylated at S139 in cells exposed to DNA double-strand break-inducing agents, such as ionizing radiation. The S139 phosphorylated H2AX, termed ( gamma -H2AX, marks the site of DNA double-strand breaks and serves to recruit cell cycle checkpoint and DNA repair factors to the site of damage.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Ca, Ma
Applications: WB, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Ch, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Bt, Bv, Ca, Fi, Gt, Pm
Applications: WB, ChIP, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC, KD, KO
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu
Applications: WB, Func, ICC/IF, IP, In vitro, KO
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: WB, Flow, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Ha, Ma, Pm
Applications: WB, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP, ISH, PAGE, KO
Species: Hu, Mu, Rt(-)
Applications: WB, Flow, ICC/IF, Flow-IC
Species: Hu, Mu
Applications: WB, ICC/IF, IP
Species: Hu
Applications: WB, Simple Western, IHC
Species: Hu
Applications: WB, Flow, ICC/IF, IP, CyTOF-ready
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, IHC, IHC-P, IP
Species: Hu
Applications: WB, IHC
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl, KD, KO

Publications for Histone H2AX Antibody (AF2288)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Goat.

We have publications tested in 3 applications: ICC, IHC, Western Blot.

Filter By Application
Western Blot
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF2288 Applications Species
HL Li, CY Wang, J Fu, XJ Yang, Y Sun, YH Shao, LH Zhang, XM Yang, XL Zhang, J Lin PTEN expression in U251 glioma cells enhances their sensitivity to ionizing radiation by suppressing DNA repair capacity Eur Rev Med Pharmacol Sci, 2019;23(23):10453-10458. 2019 [PMID: 31841199] (ICC, Human) ICC Human
Y Wang, AJ Bernhardy, J Nacson, JJ Krais, YF Tan, E Nicolas, MR Radke, E Handorf, A Llop-Gueva, J Balmaña, EM Swisher, V Serra, S Peri, N Johnson BRCA1 intronic Alu elements drive gene rearrangements and PARP inhibitor resistance Nat Commun, 2019;10(1):5661. 2019 [PMID: 31827092] (ICC, Human) ICC Human
Z Simandi, K Pajer, K Karolyi, T Sieler, LL Jiang, Z Kolostyak, Z Sari, Z Fekecs, A Pap, A Patsalos, GA Contreras, B Reho, Z Papp, X Guo, A Horvath, G Kiss, Z Keresztess, G Vámosi, J Hickman, H Xu, D Dormann, T Hortobagyi, M Antal, A Nogradi, L Nagy Arginine methyltransferase PRMT8 provides cellular stress tolerance in aging motoneurons J. Neurosci., 2018;0(0):. 2018 [PMID: 30054395] (Western Blot, Mouse) Western Blot Mouse
M Jangi, C Fleet, P Cullen, SV Gupta, S Mekhoubad, E Chiao, N Allaire, CF Bennett, F Rigo, AR Krainer, JA Hurt, JP Carulli, JF Staropoli SMN deficiency in severe models of spinal muscular atrophy causes widespread intron retention and DNA damage Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28270613] (IHC, Mouse) IHC Mouse
N Rebolleda, I Losada-Fer, G Perez-Chac, R Castejon, S Rosado, M Morado, MT Vallejo-Cr, A Martinez, JA Vargas-Nuñ, P Perez-Acie Synergistic Activity of Deguelin and Fludarabine in Cells from Chronic Lymphocytic Leukemia Patients and in the New Zealand Black Murine Model PLoS ONE, 2016;11(4):e0154159. 2016 [PMID: 27101369] (Western Blot, Human) Western Blot Human
Kim, Bo S, Serebreni, Leonid, Fallica, Jonathan, Hamdan, Omar, Wang, Lan, Johnston, Laura, Kolb, Todd, Damarla, Mahendra, Damico, Rachel, Hassoun, Paul M Cyclin-dependent kinase five mediates activation of lung xanthine oxidoreductase in response to hypoxia. PLoS ONE, 2015;10(4):e0124189. 2015 [PMID: 25831123]
Jia XZ, Yang SY, Zhou J, Li SY, Ni JH, An GS, Jia HT Inhibition of CHK1 kinase by Go6976 converts 8-chloro-adenosine-induced G2/M arrest into S arrest in human myelocytic leukemia K562 cells. Biochem. Pharmacol., 2009;77(5):770-80. 2009 [PMID: 19059218] (Western Blot, Human) Western Blot Human
Yang SY, Jia XZ, Feng LY, Li SY, An GS, Ni JH, Jia HT Inhibition of topoisomerase II by 8-chloro-adenosine triphosphate induces DNA double-stranded breaks in 8-chloro-adenosine-exposed human myelocytic leukemia K562 cells. Biochem. Pharmacol., 2009;77(3):433-43. 2009 [PMID: 19014910] (Western Blot, Human) Western Blot Human
Roh DS, Cook AL, Rhee SS, Joshi A, Kowalski R, Dhaliwal DK, Funderburgh JL DNA cross-linking, double-strand breaks, and apoptosis in corneal endothelial cells after a single exposure to mitomycin C. Invest. Ophthalmol. Vis. Sci., 2008;49(11):4837-43. 2008 [PMID: 18658091] (ICC, Goat) ICC Goat
Tang KF, He CX, Zeng GL, Wu J, Song GB, Shi YS, Zhang WG, Huang AL, Steinle A, Ren H Induction of MHC class I-related chain B (MICB) by 5-aza-2'-deoxycytidine. Biochem. Biophys. Res. Commun., 2008;370(4):578-83. 2008 [PMID: 18395517] (ICC, Human) ICC Human
Show All 11 Publications.

Reviews for Histone H2AX Antibody (AF2288) (0)

There are no reviews for Histone H2AX Antibody (AF2288). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Histone H2AX Antibody (AF2288) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Additional Histone H2AX Products

Bioinformatics Tool for Histone H2AX Antibody (AF2288)

Discover related pathways, diseases and genes to Histone H2AX Antibody (AF2288). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Histone H2AX Antibody (AF2288)

Discover more about diseases related to Histone H2AX Antibody (AF2288).

Pathways for Histone H2AX Antibody (AF2288)

View related products by pathway.

PTMs for Histone H2AX Antibody (AF2288)

Learn more about PTMs related to Histone H2AX Antibody (AF2288).

Blogs on Histone H2AX

There are no specific blogs for Histone H2AX, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

53BP1 Antibody

Contact Information

Learn the difference between western blot and simple western

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Histone H2AX [p Ser139] Antibody [Unconjugated] and receive a gift card or discount.


Gene Symbol H2AFX