gamma H2AX Products

Antibodies
gamma H2AX [p Ser139] Antibod ...
gamma H2AX [p Ser139] Antibody
NB100-2280
Species: Hu, Mu, Ca, Po, Bt, Bv, ChHa, Gp, Pm, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Host: Rabbit Polyclonal
gamma H2AX [p Ser139] Antibod ...
gamma H2AX [p Ser139] Antibody (3F2)
NB100-74435
Species: Hu, Mu, Bv
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P
Host: Mouse Monoclonal

Description

Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). The H2AX variant represents between 2-25% of total H2A (1-3). Human H2AX protein is 143 amino acids in length and has a theoretical molecular weight of 15 kDa (5). As a result of DSBs, H2AX becomes phosphorylated at serine-139 and gamma H2AX is generated as a response to genomic instability (1-4). Gamma H2AX and its phosphorylation can be detected through western blotting and immunofluorescence, serving as a biomarker for DSB frequency (1-4). Ataxia telangiectasia mutated (ATM) is considered a key protein for H2AX phosphorylation (1-4). Upon the phosphorylation and formation of gamma H2AX, many repair proteins become associated including breast cancer 1 protein (BRCA1), NBS1 (Nijmegen breakage syndrome 1)/hMRE11 (human meiotic recombination 11 protein)/ hRAD50 (N/M/R) repair complex, and 53BP1 (p53 binding protein 1) (1-4). Gamma H2AX detection assays are currently utilized for a variety of purposes, from studying DNA repair to drug development and radiation biodosimetry (4).

References

1. Palla, V. V., Karaolanis, G., Katafigiotis, I., Anastasiou, I., Patapis, P., Dimitroulis, D., & Perrea, D. (2017). gamma-H2AX: Can it be established as a classical cancer prognostic factor?. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. https://doi.org/10.1177/1010428317695931

2. Kuo, L. J., & Yang, L. X. (2008). Gamma-H2AX - a novel biomarker for DNA double-strand breaks. In vivo (Athens, Greece).

3. Kinner, A., Wu, W., Staudt, C., & Iliakis, G. (2008). Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic acids research. https://doi.org/10.1093/nar/gkn550

4. Redon, C. E., Weyemi, U., Parekh, P. R., Huang, D., Burrell, A. S., & Bonner, W. M. (2012). gamma-H2AX and other histone post-translational modifications in the clinic. Biochimica et biophysica acta. https://doi.org/10.1016/j.bbagrm.2012.02.021

5. H2AX: Uniprot (P16104)

Bioinformatics

Entrez Mouse
Human
Rat
Uniprot Human
Human
Product By Gene ID 3014
Alternate Names
  • gH2AX
  • H2A histone family member X
  • H2A histone family, member X
  • H2A.X
  • H2A/X
  • H2a/x
  • H2AFX
  • H2AX histone
  • H2AX
  • Histone H2A.x
  • Histone H2AX
  • YH2AX

Research Areas for gamma H2AX

Find related products by research area and learn more about each of the different research areas below.

Checkpoint signaling
DNA Double Strand Break Repair
DNA Repair
Mitotic Regulators
Phospho-Specific

Bioinformatics Tool for gamma H2AX

Discover related pathways, diseases and genes to gamma H2AX. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
 
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Related gamma H2AX Blog Posts

Check out the latest blog posts on gamma H2AX.
Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...    Read more.
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