IFI202b Antibody

Western Blot: IFI202b Antibody [NBP2-27066] - Analysis of Ifi202b in mouse spleen tissue lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

IFI202b Antibody Summary

• Immunogen: A synthetic peptide corresponding to amino acids 273-290 of the mouse Ifi202b protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IFI202B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000

Reactivity Notes

Mouse. Human reactivity reported in scientific literature (PMID: 24131791).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Notes

This product shares 91% sequence homology with Human IFI16

Background

Interferon-activable protein 202 (Ifi202b, p202) belongs to the HIN-200 family and contains two HIN-200 domains. It is reported to inhibit transcriptional activity of several transcription factors, including NF-kappaB p50 and p65, FOS, JUN, E2F1, E2F4, MYOD1 and myogenin. It has anti-apoptotic effects due to inhibition of the transcriptional activity of p53. It is an interferon-inducible protein which is responsible for Lupus susceptibility and is induced by Interleukin-6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFI202b Antibody (NBP2-27066)(3)

Publications using NBP2-27066

• Panchanathan R, Liu H, Leung YK et al. Bisphenol A (BPA) stimulates the interferon signaling and activates the inflammasome activity in myeloid cells. Mol. Cell. Endocrinol. 2015 Aug 12 [PMID: 26277401] (WB, Mouse)
  
  Details:
  IFI202b antibody (Imgenex: IMG-6670A) was used for WB on lysates of purified CD11b+ murine bone marrow-derived cells / BMCs (from C57BL/6 female mice) that were treated or not with 10 nM of Bisphenol A/ for 14 h in vitro (Fig. 3B). WB was also performed on lysates of murine macrophages cell line J774.A1 treated or not with 10 or 100 nM of BPA. WB was also performed under similar treatment conditions on lysates of BMCs which were isolated from non lupus-prone C57BL/6 or age-matched lupus-prone [(NZB × NZW)F1] female mice (Fig 5).
• Panchanathan R, Liu H, Xin D et al. Identification of a negative feedback loop between cyclic di-GMP-induced levels of IFI16 and p202 cytosolic DNA sensors and STING. Innate Immun 2013 Oct 16 [PMID: 24131791] (WB, Human, Mouse)
• Panchanathan R, Liu H, Liu H et al. Distinct regulation of murine lupus susceptibility genes by the IRF5/Blimp-1 axis. J Immunol. 2012 Jan 1 [PMID: 22116829] (WB)
  
  Details:
  WB (mouse splenocytes or RAW 264.7 cells), Figs 1A, 2F, 3B, 3D, 6B, 6E, 7A. The specificity of the antibody was validated with recombinantly expressed mouse p202 protein by WB (see publication for details).

Bioinformatics

• Gene Symbol: IFI202B
• Entrez:
  • 26388(Mouse)
• Uniprot:
  • Q9R002(Mouse)