IFI202b Antibody - BSA Free

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Western Blot: IFI202b Antibody [NBP2-27066] - Analysis of Ifi202b in mouse spleen tissue lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using this antibody. Goat anti-rabbit ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

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IFI202b Antibody - BSA Free Summary

Immunogen
A synthetic peptide corresponding to amino acids 273-290 of the mouse Ifi202b protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IFI202B
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Publications
Read Publications using
NBP2-27066 in the following applications:

  • WB
    3 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24131791).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for IFI202b Antibody - BSA Free

  • Ifbip-1;Ifi202;Ifi202a;Ifi202c;Interferon-activable protein 202;p20;p202

Background

Interferon-activable protein 202 (Ifi202b, p202) belongs to the HIN-200 family and contains two HIN-200 domains. It is reported to inhibit transcriptional activity of several transcription factors, including NF-kappaB p50 and p65, FOS, JUN, E2F1, E2F4, MYOD1 and myogenin. It has anti-apoptotic effects due to inhibition of the transcriptional activity of p53. It is an interferon-inducible protein which is responsible for Lupus susceptibility and is induced by Interleukin-6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IFI202b Antibody (NBP2-27066)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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WB
(3)
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Human
(1)
Mouse
(2)
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Showing Publications 1 - 4 of 4.
Publications using NBP2-27066 Applications Species
Zhang L, He Y, Zhang M, Zhang J et Al. Heterozygous Gnaq deficiency enhances Ifi202b/IFI16 and NF-?B activation in endothelial cells and exacerbates lupus nephritis pathology iScience 2024-08-07 [PMID: 39108722]
Panchanathan R, Liu H, Leung YK et al. Bisphenol A (BPA) stimulates the interferon signaling and activates the inflammasome activity in myeloid cells. Mol. Cell. Endocrinol. 2015-08-12 [PMID: 26277401] (WB, Mouse)

Details:
IFI202b antibody (Imgenex: IMG-6670A) was used for WB on lysates of purified CD11b+ murine bone marrow-derived cells / BMCs (from C57BL/6 female mice) that were treated or not with 10 nM of Bisphenol A/ for 14 h in vitro (Fig. 3B). WB was also performed on lysates of murine macrophages cell line J774.A1 treated or not with 10 or 100 nM of BPA. WB was also performed under similar treatment conditions on lysates of BMCs which were isolated from non lupus-prone C57BL/6 or age-matched lupus-prone [(NZB × NZW)F1] female mice (Fig 5).
WB Mouse
Panchanathan R, Liu H, Xin D, Choubey D. Identification of a negative feedback loop between cyclic di-GMP-induced levels of IFI16 and p202 cytosolic DNA sensors and STING. Innate Immun. [PMID: 24131791] (WB, Mouse, Human) WB Mouse, Human
Panchanathan R, Liu H, Liu H et al. Distinct regulation of murine lupus susceptibility genes by the IRF5/Blimp-1 axis. J Immunol. 2012-01-01 [PMID: 22116829] (WB)

Details:
WB (mouse splenocytes or RAW 264.7 cells), Figs 1A, 2F, 3B, 3D, 6B, 6E, 7A. The specificity of the antibody was validated with recombinantly expressed mouse p202 protein by WB (see publication for details).
WB

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Bioinformatics

Gene Symbol IFI202B
Entrez
Uniprot