Human Kidney Whole Tissue Lysate (Adult Whole Tumor)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Concentration
5.0 mg/ml

Order Details

Human Kidney Whole Tissue Lysate (Adult Whole Tumor) Summary

Specificity
Total Protein - Human Tumor Tissue: Kidney

Applications/Dilutions

Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40 and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Kidney

Type
Tissue
Life Stage
Adult
Tissue
Digestive/Waste
Protein State
Native
Tissue Condition
Tumor
Subcellular Fraction
Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Kidney Lysate (NB820-59429). (Showing 1 - 3 of 3 FAQ).

  1. What are the protease inhibitors? How much KCL, EDTA, magnesium chloride, sucrose, HEPES, glycerol is used?
    • The information you have requested is proprietary. I am sorry for any inconvenience.
  2. How is the protein concentration determined (BCA, nanodrop)?
    • The protein concentration of this lysate is determined using the Bio-Rad DC Protein Assay Kit.
  3. Is densitometry analysis performed to determine if the size of the bands of the different biomarkers tested in Western blot are the same across lots?
    • No, we do not perform densitometry to quantitate the band intensity, as long as we see an obvious band, it will pass our QC.

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