53BP1 Antibody

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-305] - Paraformaldehyde (4%) fixed Saos-2 cells (human osteosarcoma cell line) were stained with anti-53BP1 antibody followed by Alexa Fluor 488 secondary antibody. ICC/IF image submitted by a verified customer review.

Western Blot: 53BP1 Antibody [NB100-305] - Western blot of whole cell lysate (20 ug/lane) from U2Os cells resolved on a 3 to 8 percent trisacetate gel. A band appears at approximately the theoretical molecular weight of 214 kDa.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-305] - Exosomes modulate the repair of DNA DSBs in irradiated recipient cells. Representative images of 53BP1 foci in BHY cells 6 hours after 2 Gy and transfer of BHY exosomes isolated 24 hours after irradiation with 0, 3, 6 or 9 Gy (53BP1 foci green, nuclei blue). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0152213), licensed under a CC-BY licence.

Immunohistochemistry: 53BP1 Antibody [NB100-305] - Immunohistochemical staining of placental villi with 53BP1 Antibody (Catalog #NB100-305) at 40X magnification.

Flow Cytometry: 53BP1 Antibody [NB100-305] - An intracellular stain was performed on HeLa cells with 53BP1 Antibody (Catalog #NB100-305AF488) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-305] - Analysis in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-305] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated at a 1:200 dilution overnight at 4C and detected with and anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

• Reactivity: Hu, Mu, Rt, Bt, Bv, Ca
• Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

53BP1 Antibody Summary

• Immunogen: The epitope recognized by 53BP1 Antibody maps to a region between residues 1925 and the C-terminus (residue 1972) of human 53BP1 (NP_005648.1).
• Marker: DNA Double Strand Break Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TP53BP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation
  • Flow Cytometry 2-5 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1:50-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: This 53BP1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western Blot. Chromatin Immunoprecipitation and Immunohistochemistry-Frozen were reported in scientific literature.
• Control:

  Human Placenta Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Human has been tested in both WB and ICC/IF, mouse has only been tested in ICC/IF. Feedback on bovine has been negative. Rat reactivity reported in scientific literature (PMID: 24244353). Bat, canine, and bovine reactivity reported in scientific literature (PMID: 27573809). Predicted cross-reactivity based on sequence identity: Bovine (100%), Canine (100%), Chimpazee (100%), Equine (100%), Feline (100%), Gibbon (100%), Gorilla (100%), Human (100%), Hamster (100%), Marmoset (100%), Mouse (100%), Orangutan (100%), Panda (100%), Porcine (100%), Rabbit (100%), Sheep (100%), Bat (98%), Elephant (98%), Rat (98%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as secondary antibodies: NB100-1803, NB100-322, NB100-224, NB100-464, NB100-1707, NB100-2349, NB100-97827, NB500-160, NB200-171

Alternate Names for 53BP1 Antibody

• 53BP1
• p202
• p53-binding protein 1
• p53bp1
• TDRD30
• TP53-Binding Protein 1
• TP53BP1
• tumor protein 53-binding protein, 1
• tumor protein p53 binding protein 1
• tumor protein p53-binding protein, 1
• tumor suppressor p53-binding protein 1

Background

Tumor protein p53 binding protein 1 (P53-binding protein 1 or 53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase with a theoretical molecular weight of 214 kDa. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 plays a key role in response to DNA damage, acts as a signaling checkpoint during mitosis, and enhances TP53-mediated transcriptional activation. Originally identified as p53's transcriptional enhancing partner, 53BP1 is known as a key substrate for ataxia telangiectasia mutated (ATM) signaling, whose function to generate gamma H2AX may be partially compensated by the activity of DNA-dependent kinase (DNA-PK). 53BP1 relocalizes to discrete foci overlapping with gamma phosphorylated histone H2AX; demarcating DNA double strand breaks (DSBs) sites following exposure to radiation (1). 53BP1 functions downstream of gamma H2AX-dependent proteins that collectively establish ionizing radiation induced foci at DSBs. 53BP1 is downstream of Mre11/Rad50/NBS1 (MRN complex), MDC1, RNF8, RNF168 and HERC2 which recruit 53BP1 to the DSB site, suggesting a role in DNA repair through genomic stability maintenance (2).

References

1.Henry, E., Souissi-Sahraoui, I., Deynoux, M., Lefevre, A., Barroca, V., Campalans, A.,... Arcangeli, M. L. (2019). Human hematopoietic stem/progenitor cells display ROS-dependent long-term hematopoietic defects after exposure to low dose of ionizing radiations. Haematologica. doi:10.3324/haematol.2019.226936

2.Janoshazi, A. K., Horton, J. K., Zhao, M. L., Prasad, R., Scappini, E. L., Tucker, C. J., & Wilson, S. H. (2020). Shining light on the response to repair intermediates in DNA of living cells. DNA Repair (Amst), 85, 102749. doi:10.1016/j.dnarep.2019.102749

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 53BP1 Antibody (NB100-305)(94)

Publications using NB100-305

• Nacson J, Di Marcantonio D, Wang Y et al. BRCA1 Mutational Complementation Induces Synthetic Viability Mol. Cell Apr 22 2020 [PMID: 32359443] (WB, Mouse)
• Prajapati B, Fatima M, Fatma M et al. Temporal transcriptome analysis of neuronal commitment reveals the preeminent role of the divergent lncRNA biotype and a critical candidate gene during differentiation Cell Death Discov Apr 24 2020 [PMID: 32351715] (ChIP)
• Bao X, Wang J, Zhou G et al. Extended in vitro culture of primary human mesenchymal stem cells downregulates Brca1-related genes and impairs DNA double-strand break recognition FEBS Open Bio Apr 25 2020 [PMID: 32333827] (ICC/IF, Mouse)
• Elbasani E, Gramolelli S, GUnther T et al. Kaposi sarcoma herpesvirus lytic replication is independent of the anaphase promoting complex activity J. Virol. Apr 15 2020 [PMID: 32295923] (ICC/IF, Human)
• Ercilla A, Benada J, Amitash S et al. Physiological Tolerance to ssDNA Enables Strand Uncoupling during DNA Replication Cell Rep Feb 18 2020 [PMID: 32075739] (ICC/IF, Human)
• GOttgens EL, van den Heuvel CN, de Jong MC ACLY (ATP Citrate Lyase) Mediates Radioresistance in Head and Neck Squamous Cell Carcinomas and is a Novel Predictive Radiotherapy Biomarker Cancers (Basel) Dec 7 2019 [PMID: 31817870] (ICC/IF, Human)
• Sieben CJ, Jeganathan KB, Nelson GG et al. BubR1 allelic effects drive phenotypic heterogeneity in mosaic-variegated aneuploidy progeria syndrome J. Clin. Invest. Nov 18 2019 [PMID: 31738183] (ICC/IF, Mouse)
• Callen E, Zong D, Wu W et al. 53BP1 Enforces Distinct Pre- and Post-resection Blocks on Homologous Recombination Mol. Cell 2019 Oct 15 [PMID: 31653568]
• Hladik D, Hofig I, Oestreicher U et al. Long-term culture of mesenchymal stem cells impairs ATM-dependent recognition of DNA breaks and increases genetic instability Stem Cell Res Ther Jul 29 2019 [PMID: 31358047] (ICC/IF, Mouse)
• Hwang SY, Kang MA, Baik CJ et al. CTCF cooperates with CtIP to drive homologous recombination repair of double-strand breaks Nucleic Acids Res. Jul 24 2019 [PMID: 31340001]
• Gottgens EL, Bussink J, Leszczynska KB et al. Inhibition of CD4/CDK6 enhances radiosensitivity of HPV negative head and neck squamous cell carcinomas Int. J. Radiat. Oncol. Biol. Phys. Jul 1 2019 [PMID: 31271827] (ICC/IF, Human)
• Spies J, Lukas C, Somyajit K et al. 53BP1 nuclear bodies enforce replication timing at under-replicated DNA to limit heritable DNA damage Nat. Cell Biol. Feb 25 2019 [PMID: 30804506] (ICC/IF, Human)
• Anderson R, Lagnado A, Maggiorani D et al. Length-independent telomere damage drives post-mitotic cardiomyocyte senescence EMBO J. Mar 1 2019 [PMID: 30737259] (ICC/IF, Rat)
• Zong D, Adam S, Wang Y et al. BRCA1 Haploinsufficiency Is Masked by RNF168-Mediated Chromatin Ubiquitylation. Mol. Cell Jan 10 2019 [PMID: 30704900] (ICC/IF, Human)
• Benkafadar N, Francois F, Affortit C et al. ROS-Induced Activation of DNA Damage Responses Drives Senescence-Like State in Postmitotic Cochlear Cells: Implication for Hearing Preservation. Mol. Neurobiol. Jan 28 2019 [PMID: 30693443] (ICC/IF, Mouse)
• Boscolo Sesillo F, Fox D, Sacco A. Muscle Stem Cells Give Rise to Rhabdomyosarcomas in a Severe Mouse Model of Duchenne Muscular Dystrophy. Cell Rep Jan 15 2019 [PMID: 30650360] (ICC/IF, Mouse)
• Kocher S, Beyer B, Lange T et al. A functional ex vivo assay to detect PARP1-EJ repair and radiosensitization by PARP-inhibitor in prostate cancer Int. J. Cancer Nov 26 2018 [PMID: 30478958] (IHC-Fr, Human)
• Clouaire T, Rocher V, Lashgari A et al. Comprehensive Mapping of Histone Modifications at DNA Double-Strand Breaks Deciphers Repair Pathway Chromatin Signatures. Molecular Cell Sep 1 2018 [PMID: 30270107] (ChIP, Human)
• Zhang Y, Chang JF, Sun J et al. Histone H3K27 methylation is required for NHEJ and genome stability by modulating the dynamics of FANCD2 on chromatin J. Cell. Sci. May 14 2018 [PMID: 29760279] (WB, Human)
• Parisotto M, Grelet E, El Bizri R et al. PTEN deletion in luminal cells of mature prostate induces replication stress and senescence in vivo J. Exp. Med. May 9 2018 [PMID: 29743291] (Mouse)
• Ohsaki A, Miyano Y, Tanaka R et al. A novel mechanism of gamma-irradiation-induced IL-6 production mediated by P2Y11 receptor in epidermal keratinocytes. Biol. Pharm. Bull. Mar 16 2018 [PMID: 29553088] (Human)
• Chen NC, Partridge AT, Tuzer F, Cohen J. Induction of a Senescence-Like Phenotype in Cultured Human Fetal Microglia During HIV-1 Infection. J. Gerontol. A Biol. Sci. Med. Sci. 2018 Feb 05 [PMID: 29415134] (IHC, Human)
• Santaguida S, Richardson A, Iyer DR et al. Chromosome Mis-segregation Generates Cell-Cycle-Arrested Cells with Complex Karyotypes that Are Eliminated by the Immune System. Dev. Cell. 2017 Jun 19 [PMID: 28633018]
• Vancevska A, Douglass KM, Pfeiffer V et al. The telomeric DNA damage response occurs in the absence of chromatin decompaction. Genes Dev 2017 Mar 15 [PMID: 28381410] (WB, Human)
• Reindl J, Girst S, Walsh DW et al. Chromatin organization revealed by nanostructure of irradiation induced gammaH2AX, 53BP1 and Rad51 foci Sci Rep Jan 17 2017 [PMID: 28094292] (ICC/IF, Human)
• Benkafadar N, Menardo J, Bourien J et al. Reversible p53 inhibition prevents cisplatin ototoxicity without blocking chemotherapeutic efficacy. EMBO Mol Med Oct 28 2016 [PMID: 27794029] (ICC/IF, Mouse)
• Croco E, Marchionni S, Bocchini M et al. DNA Damage Detection by 53BP1: Relationship to Species Longevity. J. Gerontol. A Biol. Sci. Med. Sci. 2016 Aug 29 [PMID: 27573809]
• Bakr A, Kocher S, Volquardsen J et al. Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1. Oncotarget. 2016 Sep 06 [PMID: 27494840]
• Morales JC, Richard P, Patidar PL et al. XRN2 Links Transcription Termination to DNA Damage and Replication Stress PLoS Genet. 2016 Jul 01 [PMID: 27437695]
• Hashizume R, Zhang A, Mueller S et al. Inhibition of DNA damage repair by the CDK4/6 inhibitor palbociclib delays irradiated intracranial atypical teratoid rhabdoid tumor and glioblastoma xenograft regrowth Neuro-oncology 2016 Jul 01 [PMID: 27370397] (IHC)
• Ochs F, Somyajit K, Altmeyer M et al. 53BP1 fosters fidelity of homology-directed DNA repair Nat. Struct. Mol. Biol. 2016 Aug 01 [PMID: 27348077]
• Penterling C, Drexler GA, Bohland C et al. Depletion of Histone Demethylase Jarid1A Resulting in Histone Hyperacetylation and Radiation Sensitivity Does Not Affect DNA Double-Strand Break Repair. PLoS ONE Jun 3 2016 [PMID: 27253695] (ICC/IF, Human)
• van Ree JH, Nam HJ, Jeganathan KB et al. Pten regulates spindle pole movement through Dlg1-mediated recruitment of Eg5 to centrosomes. Nat Cell Biol 2016 Jul [PMID: 27240320] (WB)
• Mutschelknaus L, Peters C, Winkler K et al. Exosomes Derived from Squamous Head and Neck Cancer Promote Cell Survival after Ionizing Radiation. PLoS One 2016 Mar 23 [PMID: 27006994] (WB)
• Weyemi U, Redon CE, Choudhuri R et al. The histone variant H2A.X is a regulator of the epithelial-mesenchymal transition. Nat Commun 2016 Feb 15 [PMID: 26876487] (WB)
• Kibe T, Zimmermann M, de Lange T. TPP1 Blocks an ATR-Mediated Resection Mechanism at Telomeres. Mol. Cell. 2016 Jan 21 [PMID: 26778124] (WB, Mouse)
• Szczepanski S, Hussain MS, Sur I et al. A novel homozygous splicing mutation of CASC5 causes primary microcephaly in a large Pakistani family. Hum. Genet. 2015 Nov 30 [PMID: 26621532] (WB, ICC/IF, Human)
• Bakr A, Kocher S, Volquardsen J et al. Functional crosstalk between DNA damage response proteins 53BP1 and BRCA1 regulates double strand break repair choice. Radiother Oncol 2015 Nov 23 [PMID: 26615718]
• Reindl J, Drexler GA, Girst S et al. Nanoscopic exclusion between Rad51 and 53BP1 after ion irradiation in human HeLa cells. Phys Biol. 2015 Nov 24 [PMID: 26595336] (ICC/IF, Human)
• Cristini A, Park JH, Capranico G et al. DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions. Nucleic Acids Res. 2015 Nov 17 [PMID: 26578593] (ICC/IF, Human)
• Belin BJ, Lee T, Mullins RD. DNA damage induces nuclear actin filament assembly by formin-2 and Spire-1/2 that promotes efficient DNA repair. Elife. 2015 Aug 19 [PMID: 26287480] (ICC/IF, Human)
• Yi YW, Park JS, Kwak SJ, Seong YS. Co-treatment with BEZ235 Enhances Sensitivity of BRCA1-negative Breast Cancer Cells to Olaparib Anticancer Res. 2015 Jul 01 [PMID: 26124328] (Human)
• Mathieu AL, Verronese E, Rice GI et al. PRKDC mutations associated with immunodeficiency, granuloma, and autoimmune regulator-dependent autoimmunity J. Allergy Clin. Immunol. 2015 Apr 01 [PMID: 25842288] (ICC/IF, Human)
  
  Details:
  53BP1 antibody was used for ICC-IF on early passaged primary fibroblasts from 2 unrelated patients with PRKDC mutations presenting with immunodeficiency and autoimmunity. The fibroblasts were subjected or not to X-irradiation for 1 and 24 hours prior to immunostaining analysis (Fig E1). See full text for detailed ICC-IF protocol.
• Boersma V, Moatti N, Segura-Bayona S et al. MAD2L2 controls DNA repair at telomeres and DNA breaks by inhibiting 5' end resection Nature 2015 Mar 23 [PMID: 25799990] (WB, Human)
• Walter D, Lier A, Geiselhart A et al. Exit from dormancy provokes DNA-damage-induced attrition in haematopoietic stem cells Nature. 2015 Feb 18 [PMID: 25707806] (ICC/IF, Mouse)
  
  Details:
  53BP1 antibody used for ICC/IF analysis on methanol-fixed, mouse bone marrow cells, which was then permeabilized in 0.2% Triton-X-100/PBS. The primary antibody was used at a dilution of 1:200 and incubated for overnight at 4C (Figure 1).
• Chen L, Zhu X, Zou Y et al. The topoisomerase II catalytic inhibitor ICRF-193 preferentially targets telomeres that are capped by TRF2. Am. J. Physiol., Cell Physiol. 2014 Dec 17 [PMID: 25518961] (ICC/IF, Human)
• Wang Z, Yin H, Zhang Y et al. miR-214-mediated downregulation of RNF8 induces chromosomal instability in ovarian cancer cells. Cell Cycle 2014 [PMID: 25483088] (WB)
• Dulev S, Tkach J, Lin S, Batada, NN. SET8 methyltransferase activity during the DNA double-strand break response is required for recruitment of 53BP1. EMBO Rep. 2014 Sep 24 [PMID: 25252681]
• Klement K, Goodarzi AA. DNA double strand break responses and chromatin alterations within the aging cell. Exp Cell Res. 2014 Sep 08 [PMID: 25218945]
• Jurk Diana, Wilson Caroline, Passos Joao F et al. Chronic inflammation induces telomere dysfunction and accelerates ageing in mice. Nat Commun. 2014 Jun 24 [PMID: 24960204] (Mouse)
• Lu H, Fang EF, Sykora P et al. Senescence induced by RECQL4 dysfunction contributes to Rothmund-Thomson syndrome features in mice. Cell Death Dis. 2014 May 16 [PMID: 24832598] (ICC/IF, Human)
• Clynes D, Jelinska C, Xella B et al. ATRX Dysfunction Induces Replication Defects in Primary Mouse Cells. PLoS ONE 3/21/2014 [PMID: 24651726] (ICC/IF, Mouse)
• Munch Sandra, Weidtkamp-Peters Stefanie, Klement Karolin et al. The tumor suppressor PML specifically accumulates at RPA/Rad51-containing DNA damage repair foci but is nonessential for DNA damage-induced fibroblast senescence. Mol Cell Biol. 2014 May 01 [PMID: 24615016]
• Kato K, Nakajima K, Ui A et al. Fine-Tuning of DNA Damage-Dependent Ubiquitination by OTUB2 Supports the DNA Repair Pathway Choice. Mol. Cell 2014 Feb 24 [PMID: 24560272] (ICC/IF, Human)
• Dey M, Patra S, Su LY, Segall AM. Tumor Cell Death Mediated by Peptides That Recognize Branched Intermediates of DNA Replication and Repair. PLoS One. 2013 Nov 14 [PMID: 24244353] (ICC/IF, Rat)
• Boussouar A, Barette C, Nadon R et al. Acacetin and Chrysin, Two Polyphenolic Compounds, Alleviate Telomeric Position Effect in Human Cells. Mol Ther Nucleic Acids. 2013 Aug 20 [PMID: 23962900] (ICC/IF, Human)
• Biroccio A, Cherfils-Vicini J, Augereau A et al. TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells. Nat Cell Biol 2013 Jul [PMID: 23792691] (ICC/IF, Human)
• Cousin W, Ho ML, Desai R et al. Regenerative Capacity of Old Muscle Stem Cells Declines without Significant Accumulation of DNA Damage. PLoS One 2013 May 21 [PMID: 23704914] (ICC/IF, WB, Mouse)
• Bowen S, Wangsa D, Ried T et al. Concurrent V(D)J recombination and DNA end instability increase interchromosomal trans-rearrangements in ATM-deficient thymocytes. Nucleic Acids Res 2013 Mar 6 [PMID: 23470994] (Mouse)
• Tang J, Cho NW, Cui G et al. Acetylation limits 53BP1 association with damaged chromatin to promote homologous recombination. Nat Struct Mol Biol 2013 Feb 3 [PMID: 23377543] (ChIP, Human)
• Saker J, Kriegs M, Zenker M et al. Inactivation of HNSCC Cells by 90Y-Labeled Cetuximab Strictly Depends on the Number of Induced DNA Double-Strand Breaks. J Nucl Med 2013 Jan 23 [PMID: 23345302]
• Zimmermann M, Lottersberger F, Buonomo SB et al. 53BP1 regulates DSB repair using Rif1 to control 5' end resection. Science. 2013 Feb 8 [PMID: 23306437] (WB, Mouse)
• Bock FJ, Krumschnabel G, Manzl C et al. Loss of PIDD limits NF-kB activation and cytokine production but not cell survival or transformation after DNA damage Cell Death Differ 2012 Dec 14 [PMID: 23238565] (ICC/IF, Mouse)
• Tischner D, Manzl C, Soratroi C et al. Necrosis-like death can engage multiple pro-apoptotic Bcl-2 protein family members Apoptosis 2012 Sep 13 [PMID: 22971741] (ICC/IF, Mouse)
• Jurk D, Wang C, Miwa S et al. Postmitotic neurons develop a p21-dependent senescence-like phenotype driven by a DNA damage response Aging Cell 2012 Aug 9 [PMID: 22882466] (IHC-Fr, ICC/IF, Mouse)
• Fenske AE, Glaesener S, Bokemeyer C, Thomale J, Dahm-Daphi J, Honecker F, Dartsch DC. Cisplatin resistance induced in germ cell tumour cells is due to reduced susceptibility towards cell death but not to altered DNA damage induction or repair. Cancer Lett. 2012 May 19. [PMID: 22613583] (ICC/IF, Human)
• Chapman JR, Sossick AJ, Boulton SJ et al. BRCA1-associated exclusion of 53BP1 from DNA damage sites underlies temporal control of DNA repair. J Cell Sci. 2012 Aug 1 [PMID: 22553214] (ICC/IF, Human)
• Chahwan R, van Oers JM, Avdievich E et al. The ATPase activity of MLH1 is required to orchestrate DNA double-strand breaks and end processing during class switch recombination J Exp Med 2012 Mar 26 [PMID: 22451719] (WB, Mouse)
• Lichterfeld M, Cung T, Seiss K, Rosenberg ES, Pereyra F, Yu XG. Shelterin Dysfunction and p16INK4a-Mediated Growth Inhibition in HIV-1-Specific CD8 T Cells. J Virol;86(10):5533-40. 2012 May. [PMID: 22398292] (ICC/IF, Human)
• Blackford A, Schwab R, Nieminuszcy J et al. The DNA translocase activity of FANCM protects stalled replication forks. Hum Mol Genet 2012 May 01 [PMID: 22279085] (ICC/IF, Human)
• Patro BS, Frohlich R, Bohr VA, Stevnsner T. WRN helicase regulates the ATR-CHK1-induced S-phase checkpoint pathway in response to topoisomerase-I-DNA covalent complexes. Journal of Cell Science. 2011 Dec 8. [PMID: 22159421]
• Braun F, Hamelin J, Gevaudan G, Patureau D. Development and application of an enzymatic and cell flotation treatment for the recovery of viable microbial cells from environmental matrices such as anaerobic sludge. Appl Environ Microbiol 2011 Dec [PMID: 22003005] (ICC/IF)
• Peuscher MH, Jacobs JJL. DNA-damage response and repair activities at uncapped telomeres depend on RNF8. Nat Cell Biol. 2011 Aug 21. [PMID: 21857671]
• Freund A, Patil CK, Campisi J. p38MAPK is a novel DNA damage response-independent regulator of the senescence-associated secretory phenotype. EMBO J 2011 Apr 20 [PMID: 21399611] (ICC/IF, Human)
• Augereau A, T'kint de Roodenbeke C, Simonet T et al. Telomeric damage in early stage of chronic lymphocytic leukemia correlates with shelterin dysregulation. Blood 2011 Aug 4 [PMID: 21355086] (ICC/IF, Human)
• . First do no harm. Nature 2011 Jan 13 [PMID: 21228830] (Human)
• Rodier F, Munoz DP, Teachenor R et al. DNA-SCARS: distinct nuclear structures that sustain damage-induced senescence growth arrest and inflammatory cytokine secretion. J Cell Sci;124(Pt 1):68-81. 2011 Jan 1. [PMID: 21118958]
• Nakada S, Tai I, Panier S et al. Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1. Nature. 2010 Aug 19 [PMID: 20725033] (ICC/IF, Human)
• Ramachandran S, Chahwan R, Nepal RM et al. The RNF8/RNF168 ubiquitin ligase cascade facilitates class switch recombination. Proc Natl Acad Sci U S A;107(2):809-14. 2010 Jan 12. [PMID: 20080757] (WB, ICC/IF, Mouse)
• Stewart GS, Panier S, Townsend K et al. The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling cascade at sites of DNA damage. Cell. 2009 Feb 6 [PMID: 19203578] (ICC/IF, Human)
• Ibbich N. Immunocytochemical detection of residual double-strand breaks to determine the cellular radiosensitivity under Consideration of chromatin in human fibroblasts / Immuncytochemischer Nachweis residueller Doppelstrangbruchezur Bestimmung der zellularen Strahlenempfindlichkeit unterBerucksichtigung der Chromatinstruktur bei humanen Fibroblasten. Doctor of Medicine Thesis, Medical Faculty of the University of Hamburg, 16 December 2014 Thesis. 2014 (ICC/IF, Human)
• Shao G, Patterson-Fortin J, Messick TE et al. MERIT40 controls BRCA1-Rap80 complex integrity recruitment to DNA double-str breaks. Genes; Dev;23(6):740-754. 2009 [PMID: 19261746]
• Rieske P, Augelli BJ, Stawski R et al. A population of human brain cells expressing phenotypic markers of more than one lineage can be induced in vitro to differentiate into mesenchymal cells. Exp Cell Res 2009 Feb 1 [PMID: 19061885] (ICC/IF, Human)
• Spardy N, Duensing A, Hoskins EE et al. HPV-16 E7 Reveals a Link between DNA Replication Stress, Fanconi Anemia D2 Protein, Alternative Lengthening of Telomere-Associated Promyelocytic Leukemia Bodies. Cancer Res;68(12):9954-9963. 2008 [PMID: 19047177]
• Solier S, Sordet O, Kohn KW et al. Death Receptor-Induced Activation of the Chk2- Histone H2AX-Associated DNA Damage Response Pathways. Mol Cell Biol;29(1):68-82. 2009 [PMID: 18955500]
• Palm W, Hockemeyer D, Kibe T et al. Functional dissection of human mouse POT1 proteins. Mol Cell Biol :MCB.01352-01308. 2008 [PMID: 18955498]
• Wirthner R, Wrann S, Balamurugan K et al. Impaired DNA double-str break repair contributes to chemoresistance in HIF-1{alpha}-deficient mouse embryonic fibroblasts. Carcinogenesis;29(12):2306-2316. 2008 [PMID: 18842680]
• Greubel C, Hable V, Drexler GA et al. Quantitative analysis of DNA-damage response factors after sequential ion microirradiation Radiat Environ Biophys. 2008 Nov [PMID: 18648840]
• Greubel C, Hable V, Drexler GA et al. Competition effect in DNA damage response. Radiat Environ Biophys 2008 Nov [PMID: 18648839] (ICC/IF, Human)
• Guirouilh-Barbat J, Redon C, Pommier Y. Transcription-coupled DNA Double-Str Breaks Are Mediated via the Nucleotide Excision Repair the Mre11-Rad50-Nbs1 Complex. Mol Biol Cell :E08-02-0215. 2008 [PMID: 18632984]
• Ibarra A, Schwob E, Mendez J. Excess MCM proteins protect human cells from replicative stress by licensing backup origins of replication. PNAS;105(26):8956-8961. 2008 [PMID: 18579778]
• Rakhorst HA, Tra WM, Posthumus-Van Sluijs ST et al. Quantitative analysis of radiation-induced DNA break repair in a cultured oral mucosal model. Tissue Eng 2006 Dec [PMID: 17518676] (ICC/IF, Human)
• Rothkamm, K, Balroop, S, Shekhdar, J, Fernie, P, Goh, V. Leukocyte DNA Damage after Multi-Detector Row CT: A Quantitative Biomarker of Low-Level Radiation Exposure,. 242, 244-251. 2007 [PMID: 17185671]
• Silverman, J et al. Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM 53BPI functions in the S-phase checkpoint. Genes; Development 18: 2108-2119. 2004 [PMID: 15342490]

Reviews for 53BP1 Antibody (NB100-305) (5)

Average Rating: 3.8

(Based on 5 reviews)

We have 5 reviews tested in 2 species: Human, Mouse.

reviewed by: Anonymous ICC Human 12/30/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: Saos-2 human osteosarcoma cell line
• Species: Human
• Lot: F-6

reviewed by: Anonymous ICC Human 08/08/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: Cancer Cells
• Species: Human
• Lot: F-4

Comments

• Comments: 1/500

reviewed by: Anonymous WB Human 06/30/2015

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysates
• Species: Human
• Lot: Lot A

Blocking

• Blocking Details: 4% BSA in TBST for 1h at room temperature

Primary Anitbody

• Dilution Ratio: 1:400 in TBST + 1%BSA, overnight, 4*C

Secondary Antibody

• Secondary Description: Goat anti-rabbit IgG-HRP
• Secondary Manufacturer Cat#: sc-2301
• Secondary Concentration: 1:10,000

Details

• Detection Notes: ECL

Comments

• Comments: Transfer condition: 100v constant, 90 min at 4*C.

reviewed by: Manuela Buonanno ICC Human 11/07/2013

Summary

• Application: Immunocytochemistry
• Sample Tested: AG1522 normal human fibroblasts
• Species: Human

Blocking

• Blocking Details: 1% BSA

Primary Anitbody

• Dilution Ratio: 1:200 1h

Secondary Antibody

• Secondary Description: 1 h in secondary Alexa-
• Secondary Concentration: 1h 1:200

Details

• Fixation Details: 2% paraforlmadehyde in PBS
• Wash Description: 3 times in PBS

reviewed by: Anonymous ICC Mouse 10/06/2011

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse Keratinocytes
• Species: Mouse
• Comments: It's ok!

Blocking

• Blocking Details: Blocking Buffer: 2% BSA with 2%NGS in PBS

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:250, Primary Ab Incubation Time: 2 hours, Primary Ab Incubation Temp: room temperature

Secondary Antibody

• Secondary Description: Secondary Ab: Goat anti-rabbit 594 conjugated or 488 conjugated

Comments

• Comments: It's ok!

FAQs for 53BP1 Antibody (NB100-305). (Showing 1 - 2 of 2 FAQs).

1. We're looking for anti53BP1 antibody covalently labeled with a fluorofor preferabley Cy3. Do you make it?
   • We sell 53BP1 antibodies available conjugated to DyLight 488, 550 (very similar in spectrum to Cy3) and 650. You may also purchase the unlabeled antibodies and conjugate them to the fluorophores of your choice. The catalog numbers that you may be interested in are: NB100-904R, NB100-304R, NB100-305R.
2. Hello I want to ask you for an advice in case of antibodies. In our research we focus on DNA repair foci and use your Novus antibodies for phosphorylated histone H2AX (monoclonal mouse) and protein 53BP1(polyclonal rabbit). Now we want to use antobody for phosphorylated 53BP1 protein to increase specifity and I want to ask you which from your NOVUS antibodies will be the best for us.
   • We currently stock just one antibody to phosphorylated 53BP1, which you can see at the following link: NB100-1803. This is guaranteed for detection of 53BP1 (pSer25) in human and mouse samples by WB, FLOW, ICC/IF,IHC-P, IP and PLA. As this antibody is a rabbit polyclonal, you would be unable to stain for both 53BP1 and its phosphorylated isoform in the same sample, unless you used an antibody from a different host for detection of total 53BP1 or used directly conjugated primaries. Our mouse monoclonal to 53BP1, with catalogue number NBP2-25028, is validated for detection of the human protein by WB, FLOW and IHC. Unfortunately we do not currently stock any other antibodies to phosphorylated 53BP1.

Bioinformatics

• Gene Symbol: TP53BP1