53BP1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-305] - Paraformaldehyde (4%) fixed Saos-2 cells (human osteosarcoma cell line) were stained with anti-53BP1 antibody followed by Alexa Fluor 488 secondary ...read more
Western Blot: 53BP1 Antibody [NB100-305] - Western blot of whole cell lysate (20 ug/lane) from U2Os cells resolved on a 3 to 8 percent trisacetate gel. A band appears at approximately the theoretical molecular weight of ...read more
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-305] - Exosomes modulate the repair of DNA DSBs in irradiated recipient cells. Representative images of 53BP1 foci in BHY cells 6 hours after 2 Gy and ...read more
Immunohistochemistry: 53BP1 Antibody [NB100-305] - Immunohistochemical staining of placental villi with 53BP1 Antibody (Catalog #NB100-305) at 40X magnification.
Flow Cytometry: 53BP1 Antibody [NB100-305] - An intracellular stain was performed on HeLa cells with 53BP1 Antibody (Catalog #NB100-305AF488) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-305] - Analysis in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-305] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bt, Bv, Ca, Po, Pm, Eq, Fe, Ha, Pm-Or, Pm, Rb, ShSpecies Glossary
Applications WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

53BP1 Antibody Summary

Immunogen
The epitope recognized by 53BP1 Antibody maps to a region between residues 1925 and the C-terminus (residue 1972) of human 53BP1 (NP_005648.1).
Marker
DNA Double Strand Break Marker
Predicted Species
Porcine (100%), Chimpanzee (100%), Rabbit (100%), Orangutan (100%), Feline (100%), Equine (100%), Primate (100%), Hamster (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TP53BP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation
  • Flow Cytometry 2-5 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1:50-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This 53BP1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western Blot. Chromatin Immunoprecipitation and Immunohistochemistry-Frozen were reported in scientific literature.
Control
Human Placenta Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 5 Reviews rated 3.8
using
NB100-305 in the following applications:

Publications
Read Publications using
NB100-305 in the following applications:

  • 3 publications
  • 52 publications
  • IHC
    2 publications
  • 2 publications
  • ISH
    1 publication
  • WB
    15 publications

Reactivity Notes

Human has been tested in both WB and ICC/IF, mouse has only been tested in ICC/IF. Feedback on bovine has been negative. Rat reactivity reported in scientific literature (PMID: 24244353). Bat, canine, and bovine reactivity reported in scientific literature (PMID: 27573809). Predicted cross-reactivity based on sequence identity: Chimpazee (100%), Equine (100%), Feline (100%), Gibbon (100%), Gorilla (100%), Hamster (100%), Marmoset (100%), Orangutan (100%), Panda (100%), Porcine (100%), Rabbit (100%), Sheep (100%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as secondary antibodies: NB100-1803, NB100-322, NB100-224, NB100-464, NB100-1707, NB100-2349, NB100-97827, NB500-160, NB200-171

Alternate Names for 53BP1 Antibody

  • 53BP1
  • p202
  • p53-binding protein 1
  • p53bp1
  • TDRD30
  • TP53-Binding Protein 1
  • TP53BP1
  • tumor protein 53-binding protein, 1
  • tumor protein p53 binding protein 1
  • tumor protein p53-binding protein, 1
  • tumor suppressor p53-binding protein 1

Background

Tumor protein p53 binding protein 1 (P53-binding protein 1 or 53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase with a theoretical molecular weight of 214 kDa. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 plays a key role in response to DNA damage, acts as a signaling checkpoint during mitosis, and enhances TP53-mediated transcriptional activation. Originally identified as p53's transcriptional enhancing partner, 53BP1 is known as a key substrate for ataxia telangiectasia mutated (ATM) signaling, whose function to generate gamma H2AX may be partially compensated by the activity of DNA-dependent kinase (DNA-PK). 53BP1 relocalizes to discrete foci overlapping with gamma phosphorylated histone H2AX; demarcating DNA double strand breaks (DSBs) sites following exposure to radiation (1). 53BP1 functions downstream of gamma H2AX-dependent proteins that collectively establish ionizing radiation induced foci at DSBs. 53BP1 is downstream of Mre11/Rad50/NBS1 (MRN complex), MDC1, RNF8, RNF168 and HERC2 which recruit 53BP1 to the DSB site, suggesting a role in DNA repair through genomic stability maintenance (2).

References

1.Henry, E., Souissi-Sahraoui, I., Deynoux, M., Lefevre, A., Barroca, V., Campalans, A.,... Arcangeli, M. L. (2019). Human hematopoietic stem/progenitor cells display ROS-dependent long-term hematopoietic defects after exposure to low dose of ionizing radiations. Haematologica. doi:10.3324/haematol.2019.226936

2.Janoshazi, A. K., Horton, J. K., Zhao, M. L., Prasad, R., Scappini, E. L., Tucker, C. J., & Wilson, S. H. (2020). Shining light on the response to repair intermediates in DNA of living cells. DNA Repair (Amst), 85, 102749. doi:10.1016/j.dnarep.2019.102749

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF7114
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Publications for 53BP1 Antibody (NB100-305)(100)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ChIP, ICC/IF, IHC, IHC-Fr, ISH, WB.


Filter By Application
ChIP
(3)
ICC/IF
(52)
IHC
(2)
IHC-Fr
(2)
ISH
(1)
WB
(15)
All Applications
Filter By Species
Human
(42)
Mouse
(23)
Rat
(2)
All Species
Showing Publications 1 - 10 of 100. Show All 100 Publications.
Publications using NB100-305 Applications Species
Elsesy ME, Oh-Hohenhorst SJ, LOser A et al. Second-Generation Antiandrogen Therapy Radiosensitizes Prostate Cancer Regardless of Castration State through Inhibition of DNA Double Strand Break Repair Cancers Aug 31 2020 [PMID: 32878283] (ICC/IF, Human) ICC/IF Human
Gerakopoulos V, Ngo P, Tsiokas L Loss of polycystins suppresses deciliation via the activation of the centrosomal integrity pathway Life Sci Alliance Sep 1 2020 [PMID: 32651191] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Malaquin N, Vancayseele A, Gilbert S et al. DNA Damage- But Not Enzalutamide-Induced Senescence in Prostate Cancer Promotes Senolytic Bcl-xL Inhibitor Sensitivity Cells Jul 1 2020 [PMID: 32630281]
de Krijger I, van der Torre J, Peuscher MH et al. H3K36 dimethylation by MMSET promotes classical non-homologous end-joining at unprotected telomeres Oncogene May 29 2020 [PMID: 32472076] (ISH, ICC/IF, Mouse) ISH, ICC/IF Mouse
Nacson J, Di Marcantonio D, Wang Y et al. BRCA1 Mutational Complementation Induces Synthetic Viability Mol. Cell Apr 22 2020 [PMID: 32359443] (WB, Mouse) WB Mouse
Prajapati B, Fatima M, Fatma M et al. Temporal transcriptome analysis of neuronal commitment reveals the preeminent role of the divergent lncRNA biotype and a critical candidate gene during differentiation Cell Death Discov Apr 24 2020 [PMID: 32351715] (ChIP) ChIP
Bao X, Wang J, Zhou G et al. Extended in vitro culture of primary human mesenchymal stem cells downregulates Brca1-related genes and impairs DNA double-strand break recognition FEBS Open Bio Apr 25 2020 [PMID: 32333827] (ICC/IF, Mouse) ICC/IF Mouse
Elbasani E, Gramolelli S, GUnther T et al. Kaposi sarcoma herpesvirus lytic replication is independent of the anaphase promoting complex activity J. Virol. Apr 15 2020 [PMID: 32295923] (ICC/IF, Human) ICC/IF Human
Lototska L, Yue J X et al. Human RAP1 specifically protects telomeres of senescent cells from DNA damage. EMBO Rep Mar 4 2020 [PMID: 32096305] (ICC/IF, Human) ICC/IF Human
Ercilla A, Benada J, Amitash S et al. Physiological Tolerance to ssDNA Enables Strand Uncoupling during DNA Replication Cell Rep Feb 18 2020 [PMID: 32075739] (ICC/IF, Human) ICC/IF Human
Show All 100 Publications.

Reviews for 53BP1 Antibody (NB100-305) (5) 3.85

Average Rating: 3.8
(Based on 5 reviews)
We have 5 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-305:
Filter by Applications
ICC
(4)
WB
(1)
All Applications
Filter by Species
Human
(4)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry 53BP1 NB100-305
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5
reviewed by:
Anonymous
ICC Human 12/30/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedSaos-2 human osteosarcoma cell line
SpeciesHuman
LotF-6
Immunocytochemistry 53BP1 NB100-305
Enlarge
5
reviewed by:
Anonymous
ICC Human 08/08/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedCancer Cells
SpeciesHuman
LotF-4

Comments

Comments1/500
Western Blot 53BP1 NB100-305
Enlarge
4
reviewed by:
Anonymous
WB Human 06/30/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates
SpeciesHuman
LotLot A

Blocking

Blocking Details4% BSA in TBST for 1h at room temperature

Primary Anitbody

Dilution Ratio1:400 in TBST + 1%BSA, overnight, 4*C

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG-HRP
Secondary Manufacturer Cat#sc-2301
Secondary Concentration1:10,000

Details

Detection NotesECL

Comments

CommentsTransfer condition: 100v constant, 90 min at 4*C.
  2
reviewed by:
Manuela Buonanno
ICC Human 11/07/2013
View

Summary

ApplicationImmunocytochemistry
Sample TestedAG1522 normal human fibroblasts
SpeciesHuman

Blocking

Blocking Details1% BSA

Primary Anitbody

Dilution Ratio1:200 1h

Secondary Antibody

Secondary Description1 h in secondary Alexa-
Secondary Concentration1h 1:200

Details

Fixation Details2% paraforlmadehyde in PBS
Wash Description3 times in PBS
  3
reviewed by:
Anonymous
ICC Mouse 10/06/2011
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse Keratinocytes
SpeciesMouse
CommentsIt's ok!

Blocking

Blocking DetailsBlocking Buffer: 2% BSA with 2%NGS in PBS

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:250, Primary Ab Incubation Time: 2 hours, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti-rabbit 594 conjugated or 488 conjugated

Comments

CommentsIt's ok!

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for 53BP1 Antibody (NB100-305). (Showing 1 - 2 of 2 FAQs).

  1. We're looking for anti53BP1 antibody covalently labeled with a fluorofor preferabley Cy3. Do you make it?
    • We sell 53BP1 antibodies available conjugated to DyLight 488, 550 (very similar in spectrum to Cy3) and 650. You may also purchase the unlabeled antibodies and conjugate them to the fluorophores of your choice. The catalog numbers that you may be interested in are: NB100-904R, NB100-304R, NB100-305R.
  2. Hello I want to ask you for an advice in case of antibodies. In our research we focus on DNA repair foci and use your Novus antibodies for phosphorylated histone H2AX (monoclonal mouse) and protein 53BP1(polyclonal rabbit). Now we want to use antobody for phosphorylated 53BP1 protein to increase specifity and I want to ask you which from your NOVUS antibodies will be the best for us.
    • We currently stock just one antibody to phosphorylated 53BP1, which you can see at the following link: NB100-1803. This is guaranteed for detection of 53BP1 (pSer25) in human and mouse samples by WB, FLOW, ICC/IF,IHC-P, IP and PLA. As this antibody is a rabbit polyclonal, you would be unable to stain for both 53BP1 and its phosphorylated isoform in the same sample, unless you used an antibody from a different host for detection of total 53BP1 or used directly conjugated primaries. Our mouse monoclonal to 53BP1, with catalogue number NBP2-25028, is validated for detection of the human protein by WB, FLOW and IHC. Unfortunately we do not currently stock any other antibodies to phosphorylated 53BP1.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-305AF350
Alexa Fluor 405 NB100-305AF405
Alexa Fluor 488 NB100-305AF488
Alexa Fluor 594 NB100-305AF594
Alexa Fluor 647 NB100-305AF647
Alexa Fluor 700 NB100-305AF700
Alexa Fluor 750 NB100-305AF750
Biotin NB100-305B
DyLight 350 NB100-305UV
DyLight 405 NB100-305V
DyLight 488 NB100-305G
DyLight 550 NB100-305R
DyLight 650 NB100-305C
DyLight 680 NB100-305FR
DyLight 755 NB100-305IR
FITC NB100-305F
HRP NB100-305H
Janelia Fluor 549 NB100-305JF549
Janelia Fluor 646 NB100-305JF646

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Blogs on 53BP1.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

53BP1, DNA Damage Response and Tumor Suppression
53BP1 (p53 binding protein 1) was originally thought to be a p53 transcriptional enhancing partner, but now has been shown to be an ataxia telangiectasia mutated (ATM) substrate. It is a late DNA damage response (DDR) marker, appearing in the telophas...  Read full blog post.

53BP1, DNA Damage Response and Tumor Suppression
53BP1 (p53 binding protein 1) was originally thought to be a p53 transcriptional enhancing partner, but now has been shown to be an ataxia telangiectasia mutated (ATM) substrate. It is a late DNA damage response (DDR) marker, appearing in the telophas...  Read full blog post.

NUP153 & 53BP1: A Novel DNA Repair Pathway
Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De...  Read full blog post.

Blocking 53BP1 Expression Lessens Tumor Development in BRCA1-Defective Mice
Our antibody database at Novus Biologicals provides research tools for the forefront of cancer research. Recently, a mouse study using 53BP1 and BRCA1 antibodies showed that deletion of 53BP1 greatly lessened the incidence of tumor development in mice...  Read full blog post.

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Recent Reviews

3.8
5
2
4
1
3
1
2
1
1
0

Anonymous
12/30/2019
Application: ICC
Species: Human

Anonymous
08/08/2019
Application: ICC
Species: Human

Anonymous
06/30/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol TP53BP1