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53BP1 Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-305] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Genetic Strategies: Knockdown Validated: 53BP1 Antibody [NB100-305] - Western blot showing an efficient siRNA-mediated knockdown of the indicated individual or combined proteins. Image collected and cropped by ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody - BSA Free [NB100-305] - 53BP1 Antibody [NB100-305] - Exosomes modulate the repair of DNA DSBs in irradiated recipient cells. ...read more
Immunohistochemistry: 53BP1 Antibody [NB100-305] - Immunohistochemical staining of placental villi with 53BP1 Antibody (Catalog #NB100-305) at 40X magnification.
Western Blot: 53BP1 Antibody [NB100-305] - Western blot of whole cell lysate (20 ug/lane) from U2Os cells resolved on a 3 to 8 percent trisacetate gel. A band appears at approximately the theoretical molecular weight of ...read more
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-305] - HCC1937 cells (human) were treated with PARPi for 96 h. Nuclei are stained in blue (DAPI) and 53BP1 antibody (foci in green). ICC/IF image submitted ...read more
Flow Cytometry: 53BP1 Antibody [NB100-305] - An intracellular stain was performed on Ntera2 cells with 53BP1 Antibody NB100-305 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and ...read more
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-305] - Paraformaldehyde (4%) fixed Saos-2 cells (human osteosarcoma cell line) were stained with anti-53BP1 antibody followed by Alexa Fluor 488 secondary ...read more
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [NB100-305] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Flow Cytometry: 53BP1 Antibody [NB100-305] - An intracellular stain was performed on HeLa cells with 53BP1 Antibody (Catalog #NB100-305AF488) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Inhibition of ACLY exacerbates radiation induced DNA damage in HNSCC cells and impairs homologous recombination. (C,D) Quantification of cells positive for 53BP1 foci (>5 53BP1 foci/nucleus in a single plane of view) in ...read more
Exosomes modulate the repair of DNA DSBs in irradiated recipient cells. (B) Representative images of 53BP1 foci in BHY cells 6 hours after 2 Gy and transfer of BHY exosomes isolated 24 hours after irradiation with 0, 3, ...read more
RAP1 protects telomeres against DNA damageWestern blotting showing the expression of RAP1 and TRF2 in MRC-5 cells of different population doublings (PD). Senescent cells (PD 72) were left in culture for at least 4 weeks ...read more
Hypoxia, blood vessel and proliferation staining. IHC staining in the Biopsy 23 and Biopsy 3 respectively for CA-9, EF5, CD31, Ki67 and LYVE1. 15× Magnification. Image collected and cropped by CiteAb from the following ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bt, Bv, Ca, Nm, Po, Pm, Eq, Fe, Ha, Pm-Or, Pm, Rb, ShSpecies Glossary
Applications WB, ChIP, Flow, ICC/IF, IHC, ChIP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

53BP1 Antibody - BSA Free Summary

Immunogen
The epitope recognized by 53BP1 Antibody maps to a region between residues 1925 and the C-terminus (residue 1972) of human 53BP1 (NP_005648.1).
Marker
DNA Double Strand Break Marker
Predicted Species
Porcine (100%), Chimpanzee (100%), Rabbit (100%), Orangutan (100%), Feline (100%), Equine (100%), Primate (100%), Hamster (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TP53BP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation reported in scientific literature
  • Flow Cytometry 2-5 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1:50-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen reported in scientific literature
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:2000-1:10000
Control
Human Placenta Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 6 Reviews rated 4
using
NB100-305 in the following applications:

Publications
Read Publications using
NB100-305 in the following applications:

Reactivity Notes

Use in Naked mole-rat reported in scientific literature (PMID: 33608273). Human has been tested in both WB and ICC/IF, mouse has only been tested in ICC/IF. Feedback on bovine has been negative. Rat reactivity reported in scientific literature (PMID: 24244353). Bat, canine, and bovine reactivity reported in scientific literature (PMID: 27573809). Predicted cross-reactivity based on sequence identity: Chimpazee (100%), Equine (100%), Feline (100%), Gibbon (100%), Gorilla (100%), Hamster (100%), Marmoset (100%), Orangutan (100%), Panda (100%), Porcine (100%), Rabbit (100%), Sheep (100%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 53BP1 Antibody - BSA Free

  • 53BP1
  • p202
  • p53-binding protein 1
  • p53bp1
  • TDRD30
  • TP53-Binding Protein 1
  • TP53BP1
  • tumor protein 53-binding protein, 1
  • tumor protein p53 binding protein 1
  • tumor protein p53-binding protein, 1
  • tumor suppressor p53-binding protein 1

Background

Tumor protein p53 binding protein 1 (P53-binding protein 1 or 53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase with a theoretical molecular weight of 214 kDa. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 plays a key role in response to DNA damage, acts as a signaling checkpoint during mitosis, and enhances TP53-mediated transcriptional activation. Originally identified as p53's transcriptional enhancing partner, 53BP1 is known as a key substrate for ataxia telangiectasia mutated (ATM) signaling, whose function to generate gamma H2AX may be partially compensated by the activity of DNA-dependent kinase (DNA-PK). 53BP1 relocalizes to discrete foci overlapping with gamma phosphorylated histone H2AX; demarcating DNA double strand breaks (DSBs) sites following exposure to radiation (1). 53BP1 functions downstream of gamma H2AX-dependent proteins that collectively establish ionizing radiation induced foci at DSBs. 53BP1 is downstream of Mre11/Rad50/NBS1 (MRN complex), MDC1, RNF8, RNF168 and HERC2 which recruit 53BP1 to the DSB site, suggesting a role in DNA repair through genomic stability maintenance (2).

References

1.Henry, E., Souissi-Sahraoui, I., Deynoux, M., Lefevre, A., Barroca, V., Campalans, A., . . . Arcangeli, M. L. (2019). Human hematopoietic stem/progenitor cells display ROS-dependent long-term hematopoietic defects after exposure to low dose of ionizing radiations. Haematologica. doi:10.3324/haematol.2019.226936

2.Janoshazi, A. K., Horton, J. K., Zhao, M. L., Prasad, R., Scappini, E. L., Tucker, C. J., & Wilson, S. H. (2020). Shining light on the response to repair intermediates in DNA of living cells. DNA Repair (Amst), 85, 102749. doi:10.1016/j.dnarep.2019.102749

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 53BP1 Antibody (NB100-305)(139)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Drosophila melanogaster, ICC/IF, IHC-P, Naked mole-rat.

We have publications tested in 9 applications: Chemotaxis, FLOW, ICC, ICC/IF, IF/IHC, IHC-Fr, IHC-P, ISH, WB.


Filter By Application
Chemotaxis
(3)
FLOW
(1)
ICC
(2)
ICC/IF
(68)
IF/IHC
(4)
IHC-Fr
(2)
IHC-P
(2)
ISH
(1)
WB
(22)
All Applications
Filter By Species
Human
(56)
Mouse
(28)
Rat
(2)
Drosophila melanogaster
(1)
ICC/IF
(1)
IHC-P
(1)
Naked mole-rat
(1)
All Species
Showing Publications 1 - 10 of 139. Show All 139 Publications.
Publications using NB100-305 Applications Species
Jacome Burbano MS, Robin JD, Bauwens S et al. Non-canonical telomere protection role of FOXO3a of human skeletal muscle cells regulated by the TRF2-redox axis Communications biology 2023-05-25 [PMID: 37231173] (ICC/IF, Human)

Details:
Dilution:1:200
ICC/IF Human
Damen M, Soroka E, Khatif H et al. Epidermal stratification is uncoupled from centrosome-dependent cell division orientation of the basal progenitors bioRxiv 2020-07-25 (ICC/IF, Mouse) ICC/IF Mouse
Krais JJ, Glass DJ, Chudoba I et al. Genetic separation of Brca1 functions reveal mutation-dependent Pol? vulnerabilities Nature communications 2023-11-24 [PMID: 38001070] (WB, Mouse) WB Mouse
Arnould C, Rocher V, Saur F et al. Chromatin compartmentalization regulates the response to DNA damage Nature 2023-11-01 [PMID: 37853125] (WB)

Details:
Dilution 1:1000
WB
Sharma AB, Ramlee MK, Kosmin J et al. C16orf72/HAPSTR1/TAPR1 functions with BRCA1/Senataxin to modulate replication-associated R-loops and confer resistance to PARP disruption Nature Communications 2023-08-17 [PMID: 37591890] (WB) WB
Jin B, Zhu J, Pan T et al. MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation Nucleic acids research 2023-07-03 [PMID: 37395406] (ICC/IF, Human)

Details:
Dilutions: 1:100
ICC/IF Human
Oetting A, Christiansen S, Gatzemeier F et al. Impaired DNA double-strand break repair and effective radiosensitization of HPV-negative HNSCC cell lines through combined inhibition of PARP and Wee1 Clinical and Translational Radiation Oncology 2023-07-01 [PMID: 37180052] (IHC-P, ICC/IF, IHC-P, ICC/IF) IHC-P, ICC/IF IHC-P, ICC/IF
Komar ZM, van Gent DC, Chakrabarty S Establishing a Microfluidic Tumor Slice Culture Platform to Study Drug Response Current protocols 2023-03-01 [PMID: 36912175] (IHC-P) IHC-P
Capala ME, Pachler KS, Lauwers I et al. Ex Vivo Functional Assay for Evaluating Treatment Response in Tumor Tissue of Head and Neck Squamous Cell Carcinoma Cancers (Basel) 2023-01-12 [PMID: 36672427]
Storchova R, Palek M, Palkova N et al. Phosphorylation of TRF2 promotes its interaction with TIN2 and regulates DNA damage response at telomeres Nucleic acids research 2023-01-18 [PMID: 36651296] (ICC/IF) ICC/IF
Show All 139 Publications.

Reviews for 53BP1 Antibody (NB100-305) (6) 46

Average Rating: 4
(Based on 6 reviews)
We have 6 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-305:
Filter by Applications
ICC
(5)
WB
(1)
All Applications
Filter by Species
Human
(5)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry 53BP1 NB100-305
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5
reviewed by:
Marina Rodriguez Candela
ICC Human 06/14/2021
View

Summary

ApplicationImmunocytochemistry
Sample Testedhuman immortalized breast cancer cells
SpeciesHuman
LotF-8

Comments

CommentsCells were fixed in 4% PFA. 53BP1 Ig was diluted 1:200 and incubated overnight at 4ºC.
Immunocytochemistry 53BP1 NB100-305
Enlarge
5
reviewed by:
Verified Customer
ICC Human 12/30/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedSaos-2 human osteosarcoma cell line
SpeciesHuman
LotF-6
Immunocytochemistry 53BP1 NB100-305
Enlarge
5
reviewed by:
Verified Customer
ICC Human 08/08/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedCancer Cells
SpeciesHuman
LotF-4

Comments

Comments1/500
Western Blot 53BP1 NB100-305
Enlarge
4
reviewed by:
Verified Customer
WB Human 06/30/2015
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysates
SpeciesHuman
LotLot A
  2
reviewed by:
Manuela Buonanno
ICC Human 11/07/2013
View

Summary

ApplicationImmunocytochemistry
Sample TestedAG1522 normal human fibroblasts
SpeciesHuman
  3
reviewed by:
Verified Customer
ICC Mouse 10/06/2011
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse Keratinocytes
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for 53BP1 Antibody (NB100-305). (Showing 1 - 2 of 2 FAQs).

  1. We're looking for anti53BP1 antibody covalently labeled with a fluorofor preferabley Cy3. Do you make it?
    • We sell 53BP1 antibodies available conjugated to DyLight 488, 550 (very similar in spectrum to Cy3) and 650. You may also purchase the unlabeled antibodies and conjugate them to the fluorophores of your choice. The catalog numbers that you may be interested in are: NB100-904R, NB100-304R, NB100-305R.
  2. Hello I want to ask you for an advice in case of antibodies. In our research we focus on DNA repair foci and use your Novus antibodies for phosphorylated histone H2AX (monoclonal mouse) and protein 53BP1(polyclonal rabbit). Now we want to use antobody for phosphorylated 53BP1 protein to increase specifity and I want to ask you which from your NOVUS antibodies will be the best for us.
    • We currently stock just one antibody to phosphorylated 53BP1, which you can see at the following link: NB100-1803. This is guaranteed for detection of 53BP1 (pSer25) in human and mouse samples by WB, FLOW, ICC/IF,IHC-P, IP and PLA. As this antibody is a rabbit polyclonal, you would be unable to stain for both 53BP1 and its phosphorylated isoform in the same sample, unless you used an antibody from a different host for detection of total 53BP1 or used directly conjugated primaries. Our mouse monoclonal to 53BP1, with catalogue number NBP2-25028, is validated for detection of the human protein by WB, FLOW and IHC. Unfortunately we do not currently stock any other antibodies to phosphorylated 53BP1.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional 53BP1 Products

Research Areas for 53BP1 Antibody (NB100-305)

Find related products by research area.

Blogs on 53BP1.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

53BP1, DNA Damage Response and Tumor Suppression
53BP1 (p53 binding protein 1) was originally thought to be a p53 transcriptional enhancing partner, but now has been shown to be an ataxia telangiectasia mutated (ATM) substrate. It is a late DNA damage response (DDR) marker, appearing in the telophas...  Read full blog post.

53BP1, DNA Damage Response and Tumor Suppression
53BP1 (p53 binding protein 1) was originally thought to be a p53 transcriptional enhancing partner, but now has been shown to be an ataxia telangiectasia mutated (ATM) substrate. It is a late DNA damage response (DDR) marker, appearing in the telophas...  Read full blog post.

NUP153 & 53BP1: A Novel DNA Repair Pathway
Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De...  Read full blog post.

Blocking 53BP1 Expression Lessens Tumor Development in BRCA1-Defective Mice
Our antibody database at Novus Biologicals provides research tools for the forefront of cancer research. Recently, a mouse study using 53BP1 and BRCA1 antibodies showed that deletion of 53BP1 greatly lessened the incidence of tumor development in mice...  Read full blog post.

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Recent Reviews

4
5
3
4
1
3
1
2
1
1
0

Marina Rodriguez Candela
06/14/2021
Application: ICC
Species: Human

Verified Customer
12/30/2019
Application: ICC
Species: Human

Verified Customer
08/08/2019
Application: ICC
Species: Human

Bioinformatics

Gene Symbol TP53BP1