CD31/PECAM-1 Antibody

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ICC/IF, IF/IHC, IHC-Fr.

Showing Publications 1 - 4 of 4.

Publications using NB100-92205	Applications	Species
Lee HJ, Jung YH, Choi GE et al. O-cyclic phytosphingosine-1-phosphate stimulates HIF1 alpha-dependent glycolytic reprogramming to enhance the therapeutic potential of mesenchymal stem cells Cell Death Dis 2019-08-05 [PMID: 31383843] (IF/IHC)	IF/IHC
Chou CW, Wang CC, Wu CP et al. Tumor cycling hypoxia induces chemoresistance in glioblastoma multiforme by upregulating the expression and function of ABCB1 Neuro Oncol 2012-09-03 [PMID: 22946104] (IF/IHC, Mouse)	IF/IHC	Mouse
Kishore M, Ma L, Cornish G et al. Primed T Cell Responses to Chemokines Are Regulated by the Immunoglobulin-Like Molecule CD31 PLoS One 2012-01-01 [PMID: 22724015] (ICC/IF, Mouse)	ICC/IF	Mouse
Hsieh CH, Wu CP, Lee HT et al. NADPH oxidase subunit 4 mediates cycling hypoxia-promoted radiation resistance in glioblastoma multiforme Free Radic Biol Med 2012-06-16 [PMID: 22713363] (ICC/IF, IHC-Fr, Mouse)	ICC/IF, IHC-Fr	Mouse

Array NB100-92205

• CD31/PECAM-1 Antibodies
• CD31/PECAM-1 Antibody Pair
• CD31/PECAM-1 ELISA Kits
• CD31/PECAM-1 Lysate
• CD31/PECAM-1 Protein
• CD31/PECAM-1 Proteins and Enzymes
• CD31/PECAM-1 ELISAs