| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-41568SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97507-0.2mg | |||
| BSA Free | NBP1-97507SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-616SS | |||
| Azide and BSA Free | NBP2-80820 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64895SS | |||
| Azide and BSA Free | NBP2-80651 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27216 | |||
| Azide and BSA Free | NBP2-25199SS | |||
| Azide and BSA Free | NBP2-27216-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71775SS | |||
| Azide and BSA Free | NBP2-80850 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB500-180SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-03495SS | |||
![Western Blot: LOX propeptide Antibody [NB110-41568] - Detection of LOPP in MC3T3-E1 cell lysate.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Western-Blot-NB110-41568-img0012.jpg "Western Blot: LOX propeptide Antibody [NB110-41568] - Detection of LOPP in MC3T3-E1 cell lysate.")
![Immunohistochemistry: LOX propeptide Antibody [NB110-41568] - LOX-PP was over-expressed in the vascular wall and in VSMC from TgLOX mice. (a) Representative immunohistochemical analysis showing LOX-PP staining (brown color) in aorta from both wild-type and TgLOX mice. The indicated areas are shown at high magnification (bars = 20 um). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-31312-w) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Immunohistochemistry-NB110-41568-img0017.jpg)
![Western Blot: LOX propeptide Antibody - BSA Free [NB110-41568] - LOX propeptide Antibody [NB110-41568] - Human VSMC were transduced with a lentiviral vector encoding for full-length LOX (pLOX; black bars), LOX-PP (pLOX-PP; grey bars) or with the corresponding empty vector (pLVX; V; white bars). (a) Immunoblots corresponding to mature LOX (mLOX; left panel) & LOX-PP (right panel). The position of the pro-enzyme (ProLOX, right panel), detected with the antibody against the propeptide. GAPDH was analyzed as a loading control. Displayed blots are not cropped from different gels or different parts of the same gel. Representative immunoblots from 3 independent experiments were shown. Image collected & cropped by Cite Ab from the following publication (//pubmed.ncbi.nlm.nih.gov/30185869/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Western-Blot-NB110-41568-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: LOX propeptide Antibody [NB110-41568] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-LOX propeptide at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Immunocytochemistry-Immunofluorescence-NB110-41568-img0015.jpg)
![Western Blot: LOX propeptide Antibody [NB110-41568] - Total protein from human and mouse brain was separated on a 12% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-LOX propeptide in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Western-Blot-NB110-41568-img0014.jpg)
![Immunohistochemistry-Paraffin: LOX propeptide Antibody [NB110-41568] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse intestine using 1:200 dilution of rabbit anti- LOX propeptide (LOX-PP) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This LOX-PP antibody generated a strong cytoplasmic staining in the epithelial and other cells with a majority of the signal localizing to luminal end of columner epithelial cells. There appears to be some nuclear positivity also which might be a nuclear variant of LOX-PP.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Immunohistochemistry-Paraffin-NB110-41568-img0011.jpg)
![Immunohistochemistry: LOX propeptide Antibody - BSA Free [NB110-41568] - Mature LOX & LOX-PP were over-expressed in the vascular wall & in VSMC from TgLOX mice. (a) Representative immunohistochemical analysis showing LOX & LOX-PP staining (brown color) in aorta from both wild-type & TgLOX mice. The indicated areas are shown at high magnification (bars = 20 µm). (b,c) Mature LOX & LOX-PP protein levels were determined by Western-blot in VSMC supernatants from these animals. The position of the pro-enzyme (ProLOX), mature LOX (mLOX) & LOX-PP forms are indicated. GAPDH was analyzed as loading control. Representative immunoblots from 3 independent experiments were shown. WT: wild-type; Tg: TgLOX. Displayed blots are not cropped from different gels or different parts of the same gel & images conform the digital image & integrity policies of the journal. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30185869), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-41568_rabbit-polyclonal-lox-propeptide-antibody-3102024162492.jpg)
![Western Blot: LOX propeptide Antibody - BSA Free [NB110-41568] - Over-expression of LOX-PP did not affect VSMC proliferation. Human VSMC were transduced with a lentiviral vector encoding for full-length LOX (pLOX; black bars), LOX-PP (pLOX-PP; grey bars) or with the corresponding empty vector (pLVX; V; white bars). (a) Immunoblots corresponding to mature LOX (mLOX; left panel) & LOX-PP (right panel) are shown. The position of the pro-enzyme (ProLOX, right panel), detected with the antibody against the propeptide, was also indicated. GAPDH was analyzed as a loading control. Displayed blots are not cropped from different gels or different parts of the same gel & images conform the digital image & integrity policies of the journal. Representative immunoblots from 3 independent experiments were shown. (b) Transduced VSMC were serum-starved for 24 h & then stimulated with 20% FCS. Cell proliferation was evaluated by the [3H]-thymidine incorporation method (left panel) or by cell count (right panel) in these cells. Results represented as mean ± SD. *P < 0.003 vs. pLVX (Kruskal-Wallis; at least n = 6). (c) [3H]-thymidine incorporation into DNA assessed in human VSMC transduced with lentiviral particles for full-length LOX (pLOX; black bars), or the corresponding empty vector (pLVX; white bars) treated or not with BAPN (500 µM). Results are represented as mean ± SD. P < 0.0001: * vs. pLVX; #vs. pLOX transduced cells (Two-way ANOVA; at least n = 9). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30185869), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-41568_rabbit-polyclonal-lox-propeptide-antibody-31020241624915.jpg)
![Western Blot: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Lane 1: MW marker; Lane 2: HO-1 (rat), (recombinant); Lane 3: HO-1 (human), (recombinant); Lane 4: HO-2 (human), (recombinant); Lane 5: MDBK cell lysate; Lane 6: Dog liver microsome; Lane 7: Mouse liver microsome.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-HO-1-1-Western-Blot-NBP1-97507-img0004.jpg "Western Blot: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Lane 1: MW marker; Lane 2: HO-1 (rat), (recombinant); Lane 3: HO-1 (human), (recombinant); Lane 4: HO-2 (human), (recombinant); Lane 5: MDBK cell lysate; Lane 6: Dog liver microsome; Lane 7: Mouse liver microsome.")
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Analysis of human spleen tissue stained with HO-1, mAb (HO-1-1) at 10ug/ml.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-HO-1-1-Immunohistochemistry-Paraffin-NBP1-97507-img0003.jpg)
![Flow Cytometry: HO-1/HMOX1/HSP32 Antibody (HO-1-1) [NBP1-97507] - Analysis of 10^6 Jurkat cells stained using HO-1 monoclonal antibody (HO-1-1), (Prod. No. ADI-OSA-110), at a concentration of 50 ug/mL](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-HO-1-1-Flow-Cytometry-NBP1-97507-img0005.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP1-31327-img0031.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - Cellular aggregates composed of equine endometrial fibroblast. Co-staining using the vimentin antibody and a cytokeratin antibody (mouse). Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunocytochemistry-NBP1-31327-img0037.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-31327] - Human primary cardiac fibroblasts stained with Vimentin (green) and smooth muscle actin (red) antibodies. Image from verified customer review.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31327-img0024.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat ovary. Vimentin antibody at 1:500.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0036.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - 293T cell lysate: A. 20 ug. B. 10 ug. C. 5 ug. D. 1 ug.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP1-31327-img0014.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, membrane was blotted with Vimentin antibody at 1:50000.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP1-31327-img0023.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded human lung adenocarcinoma. Vimentin antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0022.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Paraffin-embedded rat testis. Vimentin antibody at 1:500.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP1-31327-img0034.jpg)
![Immunoprecipitation: Vimentin Antibody [NBP1-31327] - Vimentin protein from HeLa whole cell extracts using 5 ug of Vimentin antibody. Western blot analysis was performed using Vimentin antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunoprecipitation-NBP1-31327-img0019.jpg)
![Sandwich ELISA: Vimentin Antibody [NBP1-31327] - Detection of recombinant full-length Vimentin protein using as capture antibody at concentration of 5 ug/mL as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Sandwich-ELISA-NBP1-31327-img0039.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-9102024204128.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Non-transfected (-) and transfected (+) 293T whole cell extracts (10 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-91020242010448.jpg)
![ELISA: Vimentin Antibody [NBP1-31327] - Sandwich ELISA detection of recombinant full-length Vimentin protein using the capture antibody at concentration of 5 ug/mL and NBP1-31327 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-91020242063381.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202419593530.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420131228.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein expression by immunohistochemical analysis.Sample:Paraffin-Embedded adult mouse retina. Green: Vimentin protein stained by Vimentin antibody (NBP1-31327) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420131227.jpg)
![Western Blot: Vimentin Antibody [NBP1-31327] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP1-31327) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-9102024201149.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoskeleton and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-91020242011456.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-91020242021974.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat ovary.Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420134066.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420134020.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein by immunohistochemical analysis.Sample: Paraffin-embedded dog pancreas.Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420115052.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody [NBP1-31327] - Vimentin antibody detects Vimentin protein at cytoskeleton by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Vimentin stained by Vimentin antibody (NBP1-31327) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-910202420134098.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody [NBP1-31327] - Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31327_rabbit-polyclonal-vimentin-antibody-1610202419414514.jpg)
![Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW105) [NB100-616] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B Antibody (RW105)) at 5ug/ml for 60 minutes at room temperature and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW105-Immunocytochemistry-Immunofluorescence-NB100-616-img0005.jpg "Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW105) [NB100-616] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B Antibody (RW105)) at 5ug/ml for 60 minutes at room temperature and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.")
![Western Blot: KAT3B/p300 Antibody (RW105) [NB100-616] - p300 detected in a HeLa nuclear extract using NB 100-616 (1:250). ECL: 20 minute exposure.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW105-Western-Blot-NB100-616-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW105) [NB100-616] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B/p300 Antibody (RW105) NB100-616 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW105-Immunocytochemistry-Immunofluorescence-NB100-616-img0008.jpg)
![Flow Cytometry: KAT3B/p300 Antibody (RW105) [NB100-616] - An intracellular stain was performed on RAW 246.7 cells with KAT3B/p300 antibody (RW105) NB100-616PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin. Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW105-Flow-Cytometry-NB100-616-img0006.jpg)
![Flow (Intracellular): KAT3B/p300 Antibody (RW105) [NB100-616] - An intracellular stain was performed on THP-1 cells with KAT3B/p300 (RW105) antibody NB100-616 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% Saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW105-Flow-Intracellular-NB100-616-img0003.jpg)
![Flow Cytometry: KAT3B/p300 Antibody (RW105) [NB100-616] - An intracellular stain was performed on Raw264.7 cells with KAT3B/p300 Antibody (RW105) NB100-616 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW105-Flow-Cytometry-NB100-616-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: KAT3B/p300 Antibody (RW105) [NB100-616] - 293T cells were treated with different siRNAs against p300 alone for 2 days and core histones were prepared and subjected to WB analysis as indicated. The knockdown efficiency of p300 was confirmed by WB analysis of cell lysates. Western Blot protocol is 6% gel for running, 40V overnight transfer membrane. primary Ab 1:500, secondary Ab 1:5000. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-616_mouse-monoclonal-kat3b-p300-antibody-rw105-knockdown-validated-147202311647.jpg)
![Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Analysis using Alexa Fluor (R) 488 conjugate of CD45 antibody (OX-1). Rat Splenocytes were stained with CD45 antibody (OX-1) [NB100-64895] (blue) and a matched isotype control [NBP2-27287] (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-Flow-Cytometry-NB100-64895-img0005.jpg "Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Analysis using Alexa Fluor (R) 488 conjugate of CD45 antibody (OX-1). Rat Splenocytes were stained with CD45 antibody (OX-1) [NB100-64895] (blue) and a matched isotype control [NBP2-27287] (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Analysis using the FITC conjugate of CD45 antibody (OX-1). Staining of rat spleen cells.](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-Flow-Cytometry-NB100-64895-img0004.jpg)
![Flow Cytometry: CD45 Antibody (OX-1) - BSA Free [NB100-64895] - Representative sequential gating during flow cytometry analysis of rat non-parenchymal liver cells (n = 4). Cells were labeled with antibodies to SE-1 (NB110-68095AF488), CD31, and CD45 (NB100-64895PE). LSECs were then identified as SE-1-Alexa488+ cells (e), and the biexponential CD45-PE/CD31-APC of events (f) were used to display and select the CD45+ CD31+ subsets of LSECs. FMOs (used for gating), and single antibody staining controls are shown in Additional file. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33246411/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-BSA-Free-Flow-Cytometry-NB100-64895-img0006.jpg)
![Flow Cytometry: CD45 Antibody (OX-1) [NB100-64895] - Staining of rat peripheral blood lymphocytes with mouse anti-rat CD45 antibody (OX-1) [NB100-64895].](http://images.novusbio.com/fullsize/CD45-Antibody-OX-1-Flow-Cytometry-NB100-64895-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - HeLa and COS7 cells were rinsed with PBS and fixed with -20C methanol for 5 minutes. Cells were incubated with anti-TGN38 antibody diluted 1:100 in PBS containing 0.2% BSA for 1 hour. The cells were then rinsed in PBS and incubated with goat anti-mouse IgG conjugated to rhodamine for 30 minutes in PBS containing 0.2% BSA. Following washing, cells were imaged using a Zeiss Axioplan microscope. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TGN38-Antibody-2F7-1-Immunocytochemistry-Immunofluorescence-NB300-575-img0016.jpg "Immunocytochemistry/Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - HeLa and COS7 cells were rinsed with PBS and fixed with -20C methanol for 5 minutes. Cells were incubated with anti-TGN38 antibody diluted 1:100 in PBS containing 0.2% BSA for 1 hour. The cells were then rinsed in PBS and incubated with goat anti-mouse IgG conjugated to rhodamine for 30 minutes in PBS containing 0.2% BSA. Following washing, cells were imaged using a Zeiss Axioplan microscope. ICC/IF image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Staining of TGN-38 in NRK cells.](http://images.novusbio.com/fullsize/TGN38-Antibody-2F7-1-Immunocytochemistry-Immunofluorescence-NB300-575-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Analysis of Phalloidin (green) and TGN38 (purple) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a TGN38 monoclonal antibody at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 680 goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin at a dilution of 1:300 (1 unit/mL final concentration) for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.](http://images.novusbio.com/fullsize/TGN38-Antibody-2F7-1-Immunocytochemistry-Immunofluorescence-NB300-575-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Staining in NS-1 Cells.](http://images.novusbio.com/fullsize/TGN38-Antibody-2F7-1-Immunocytochemistry-Immunofluorescence-NB300-575-img0015.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Mouse liver. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-Paraffin-NBP1-31341-img0036.jpg "Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Mouse liver. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Analysis of HO-1 in HEK293 cell lysate using anti-HO-1 antibody. WB Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Western-Blot-NBP1-31341-img0017.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Nrf2-pathway activation by hybrids: nuclear translocation and targets induction. Protein levels by Western blot. Anti-actin was used as protein loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32047434/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Western-Blot-NBP1-31341-img0032.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Human renal carcinoma . Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-Paraffin-NBP1-31341-img0019.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Rat spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-Paraffin-NBP1-31341-img0035.jpg)
![Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31341_rabbit-polyclonal-ho-1-hmox1-hsp32-antibody-91020242001087.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - HO-1/HMOX1/HSP32 antibody detects HO-1/HMOX1/HSP32 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.HO-1/HMOX1/HSP32 stained by HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31341_rabbit-polyclonal-ho-1-hmox1-hsp32-antibody-910202420104478.jpg)
![Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31341_rabbit-polyclonal-ho-1-hmox1-hsp32-antibody-910202420104459.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31341_rabbit-polyclonal-ho-1-hmox1-hsp32-antibody-161020241941452.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-27216. Staining of human PBMC. Image from verified customer review.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0007.jpg "Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-27216. Staining of human PBMC. Image from verified customer review.")
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the PE conjugate of NBP2-27216. Staining of CD4 in human PBMC using anti-CD4 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0004.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the FITC conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0011.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the DyLight 405 conjugate of NBP2-27216. Staining of CD4 in human PBMCs using anti-CD4 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0005.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the PE conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of this antibody. Green represents isotype control; red represents anti-CD4 antibody. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0009.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Allophycocyanin conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0010.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) - Azide and BSA Free [NBP2-25199] - analysis of CD4 in human PBMCs using 0.1 ug of this antibody. Secondary antibody is goat anti-mouse PE.](http://images.novusbio.com/fullsize/nbp2-25199_mouse-monoclonal-cd4-antibody-rpa-t4-imgenex-img-5916a-25ug-17720241044654.jpg)
![Western Blot: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Effect of AIF overexpression on mitochondrial fission and fusion in the cortex. Immunoblotting of OPA1 and Mitofusin 1 in the mitochondrial fraction from cortical tissue of the CL and IL hemispheres at 24 h after HI in WT and AIF Tg mice. The expression of Mitofusin 1 was significantly decreased at 24 h post-HI both in WT and AIF Tg mice, while the short form (82 kDa) and cleavage band of OPA1 were significantly increased at 24 h post-HI in both the WT and AIF Tg mice. Quantification of OPA1 and Mitofusin 1 did not show any significant differences in the CL and IL hemispheres between WT and AIF Tg mice (n=6/group). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2280-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-11E9-1H12-Western-Blot-NBP1-71775-img0014.jpg "Western Blot: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Effect of AIF overexpression on mitochondrial fission and fusion in the cortex. Immunoblotting of OPA1 and Mitofusin 1 in the mitochondrial fraction from cortical tissue of the CL and IL hemispheres at 24 h after HI in WT and AIF Tg mice. The expression of Mitofusin 1 was significantly decreased at 24 h post-HI both in WT and AIF Tg mice, while the short form (82 kDa) and cleavage band of OPA1 were significantly increased at 24 h post-HI in both the WT and AIF Tg mice. Quantification of OPA1 and Mitofusin 1 did not show any significant differences in the CL and IL hemispheres between WT and AIF Tg mice (n=6/group). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2280-z), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Antibody was tested in 3T3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-11E9-1H12-Immunocytochemistry-Immunofluorescence-NBP1-71775-img0013.jpg)
![Immunohistochemistry-Paraffin: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Analysis of Mitofusin-1 in mouse liver.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-11E9-1H12-Immunohistochemistry-Paraffin-NBP1-71775-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Analysis of Mitofusin-1 expression in (1) MEF wild-type and (2) MEF Mitofusin-1-null whole cell lysates using NBP1-71775.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-11E9-1H12-Western-Blot-NBP1-71775-img0009.jpg)
![Western Blot: Mitofusin 1 Antibody (11E9-1H12) - BSA Free [NBP1-71775] - Effect of AIF overexpression on mitochondrial fission & fusion in the cortex.a Representative immunoblots of mitochondrial fission proteins (P-DRP1 & FIS1) in the mitochondrial fraction from cortical tissue of P9 WT & AIF Tg mice under physiological conditions. Quantification of P-DRP1 & FIS1 did not show a significant difference between WT & AIF Tg mice (n = 6/group). b Representative immunoblots of mitochondrial fusion proteins (OPA1 & MFN1) in the mitochondrial fraction from cortical tissue of P9 WT & AIF Tg mice under physiological conditions. Quantification of OPA1 & MFN1 did not show any significant difference between WT & AIF Tg mice (n = 6/group). c Immunoblotting of P-DRP1and FIS1 in the mitochondrial fraction from the cortical tissue of the CL & IL hemispheres in WT & AIF Tg mice at 24 h after HI. Quantification of P-DRP1 & FIS1 did not show any significant differences in the CL & IL hemispheres between WT & AIF Tg mice (n = 6/group). d Immunoblotting of OPA1 & MFN1 in the mitochondrial fraction from cortical tissue of the CL & IL hemispheres at 24 h after HI in WT & AIF Tg mice. The expression of MFN1 was significantly decreased at 24 h post-HI both in WT & AIF Tg mice, while the short form (82 kDa) & cleavage band of OPA1 were significantly increased at 24 h post-HI in both the WT & AIF Tg mice. Quantification of OPA1 & MFN1 did not show any significant differences in the CL & IL hemispheres between WT & AIF Tg mice (n = 6/group). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32001673), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-71775_mouse-monoclonal-mitofusin-1-antibody-11e9-1h12-310202416163735.jpg)
![Western Blot: Kif2a Antibody [NB500-180] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Kif2a knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:10000 of Rabbit Anti-Human Kif2a Polyclonal Antibody (Catalog # NB500-180) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Kif2a at approximately 100 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Kif2a-Antibody-Knockout-Validated-NB500-180-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Kif2a Antibody [NB500-180] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Kif2a knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:10000 of Rabbit Anti-Human Kif2a Polyclonal Antibody (Catalog # NB500-180) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Kif2a at approximately 100 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Kif2a Antibody [NB500-180] - Analysis of Kif2a in Hela, 3T3 and PC12 lysate.](http://images.novusbio.com/fullsize/Kif2a-Antibody-Western-Blot-NB500-180-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Kif2a Antibody [NB500-180] - Kif2a antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Kif2a-Antibody-Immunocytochemistry-Immunofluorescence-NB500-180-img0005.jpg)
![Simple Western: Kif2a Antibody [NB500-180] - Lane view shows a specific band for Kif2a in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Kif2a-Antibody-Simple-Western-NB500-180-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Mouse embryonic fibroblasts, fixed with 4% PFA washed with PBS, permeabilized with 0.2% Triton X-100. Primary antibody at 1:100 in 3% BSA in PBS. Secondary antibodies incubation for 2 hours. Image from a verified customer review.](http://images.novusbio.com/fullsize/TGN38-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-03495-img0007.jpg "Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Mouse embryonic fibroblasts, fixed with 4% PFA washed with PBS, permeabilized with 0.2% Triton X-100. Primary antibody at 1:100 in 3% BSA in PBS. Secondary antibodies incubation for 2 hours. Image from a verified customer review.")
![Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32.](http://images.novusbio.com/fullsize/TGN38-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-03495-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32](http://images.novusbio.com/fullsize/TGN38-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-03495-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to TGN diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.](http://images.novusbio.com/fullsize/TGN38-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-03495-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TGN38 Antibody [NBP1-03495] - Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to TGN diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.](http://images.novusbio.com/fullsize/TGN38-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-03495-img0006.jpg)
![Western Blot: NF-H Antibody [NB300-217] - Analysis of spinal cord lysates from different species using chicken pAb to NF-H, NB300-217, dilution 1:20,000 in green: [1] protein standard (red), [2] rat, [3] mouse, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the phosphorylated from of NF-H. The protein from different species is known to have different SDS-PAGE molecular weights, with large species generally expressing larger proteins. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with this antibody. The antibody does not recognize non-phosphorylated forms of NF-H.](http://images.novusbio.com/fullsize/NF-H-Antibody-Western-Blot-NB300-217-img0007.jpg "Western Blot: NF-H Antibody [NB300-217] - Analysis of spinal cord lysates from different species using chicken pAb to NF-H, NB300-217, dilution 1:20,000 in green: [1] protein standard (red), [2] rat, [3] mouse, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the phosphorylated from of NF-H. The protein from different species is known to have different SDS-PAGE molecular weights, with large species generally expressing larger proteins. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with this antibody. The antibody does not recognize non-phosphorylated forms of NF-H.")
![Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - Imaging of Feline optic nerve at antibody dilution 1:5000. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunocytochemistry-Immunofluorescence-NB300-217-img0006.jpg)
![Immunohistochemistry Free-Floating: NF-H Antibody [NB300-217] - Analysis of a rat cerebellum section stained with NF-H antibody, dilution 1:5,000 (Red), and costained with rabbit GFAP pAb, dilution 1:5,000 Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free floating sections were stained with above antibodies. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes and other glial cells.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunohistochemistry-Free-Floating-NB300-217-img0009.jpg)
![Western Blot: NF-H Antibody [NB300-217] - Analysis of 200kDa Neurofilament Heavy expression in rat spinal cord extract. The first lane is Coomassie Brilliant Blue stained and the second lane is probed with chicken anti-Neurofilament Heavy antibody NB300-217.](http://images.novusbio.com/fullsize/NF-H-Antibody-Western-Blot-NB300-217-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - Rat neurons stained with 200kDa Neurofilament Heavy Antibody NB300-217.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunocytochemistry-Immunofluorescence-NB300-217-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - SH-SY5Y cells stained with 200kDa Neurofilament Heavy Antibody NB300-217 (red) and Fibrillarin Antibody NB300-269 (green). Nuclear DNA is stained with Hoechst dye (blue).](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunocytochemistry-Immunofluorescence-NB300-217-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - Mixed rat neuron/glial cultures stained with Neurofilament Light (NF-L) antibody NBP1-05217 [green] and Neurofilament Heavy (NF-H) antibody NB300-217 [red]. Blue is a DNA stain. NB300-217 binds primarily to the phosphorylated axonal forms of NF-H, in contrast to NBP1-05217 which stains both axonal and dendritic/perikaryal neurofilaments. The surrounding axonal profiles are orange due to staining of both the NF-H and NF-L antibodies.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunocytochemistry-Immunofluorescence-NB300-217-img0004.jpg)
![Immunohistochemistry-Frozen: NF-H Antibody [NB300-217] - Canine optic nerve, antibody specifically labelled the axons (Green). Blue is DAPI. Image taken with an epifluorescent microscope and was incubated at 1:10000 for 1hr at RT.. Image from verified customer review.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunohistochemistry-Frozen-NB300-217-img0008.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody [NB300-217] - NF-H immunoreactivity in a FFPE section of mouse trigeminal ganglion. Antibody was diluted 1 to 2000 and left on sections for 1h at room temperature. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nb300-217_chicken-polyclonal-nf-h-antibody-immunohistochemistry-paraffin-204202311828.jpg)
![Immunohistochemistry-Frozen: Chicken Polyclonal NF-H Antibody [NB300-217] - Fixed frozen section of mouse optic nerve showing neurofilament heavy NB300-217 in green and S100B in red. NB300-217 was diluted 1 in 4000 and was left on tissue sections overnight at room temperature. Primary was detected with donkey anti chicken conjugated to Alexa 488. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb300-217_chicken-polyclonal-nf-h-antibody-immunohistochemistry-frozen-842024131739..png)
![Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of canine cecum showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb300-217_chicken-polyclonal-nf-h-antibody-immunohistochemistry-paraffin-2942024113042.png)
![Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of feline colon showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb300-217_chicken-polyclonal-nf-h-antibody-immunohistochemistry-paraffin-2942024113519.png)
![Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of equine jejunum showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb300-217_chicken-polyclonal-nf-h-antibody-immunohistochemistry-paraffin-2942024114043..png)
![Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of human colon showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb300-217_chicken-polyclonal-nf-h-antibody-immunohistochemistry-paraffin-2942024114432..png)