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Palladin Antibody (1E6) [DyLight 680]

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Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
1E6
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 680

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP1-25959AF405
Alexa Fluor 488
NBP1-25959AF488
Alexa Fluor 647
NBP1-25959AF647
Alexa Fluor 700
NBP1-25959AF700
Biotin
NBP1-25959B
DyLight 350
NBP1-25959UV
DyLight 405
NBP1-25959V
DyLight 488
NBP1-25959G
DyLight 550
NBP1-25959R
DyLight 650
NBP1-25959C
DyLight 755
NBP1-25959IR
FITC
NBP1-25959F
HRP
NBP1-25959H
Janelia Fluor 549
NBP1-25959JF549
Janelia Fluor 646
NBP1-25959JF646
Unconjugated
NBP1-25959
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Palladin Antibody (1E6) [DyLight 680] Summary

Immunogen
Endogenous Palladin immunoprecipitated from chicken embryo fibroblasts.
Localization
Cytoplasm, cytoskeleton. Note: Localizes to stress fibers and Z-disks. Cell junction, focal adhesion. Cell projection, ruffle. Cell projection, lamellipodium.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PALLD
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This Palladin (1E6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB a band is seen at ~85 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken, Human, Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Palladin Antibody (1E6) [DyLight 680]

  • CGI151
  • CGI-151
  • FLJ22190
  • FLJ38193
  • KIAA0992FLJ39139
  • MYN
  • myoneurin
  • Palladin
  • palladin, cytoskeletal associated protein
  • PALLD
  • PNCA1
  • Sarcoma antigen NY-SAR-77
  • SIH002
  • SIH002FLJ61376

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Myotilin Antibody [NBP2-38361] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: Myotilin Antibody [NBP2-38361] - Staining of human skin shows low expression as expected.
Immunohistochemistry-Paraffin: Myotilin Antibody [NBP2-38361] - Staining of human skeletal muscle shows high expression.
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Western Blot: VASP Antibody [NBP1-85792] - Analysis in human cell line HEL.
Immunocytochemistry/Immunofluorescence: VASP Antibody [NBP1-85792] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & focal adhesion sites. Antibody staining is shown in green.
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Immunohistochemistry-Paraffin: MYPN Antibody [NBP2-38924] - Staining in human skeletal muscle and prostate tissues using anti-MYPN antibody. Corresponding MYPN RNA-seq data are presented for the same tissues.
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Western Blot: p19ARF Antibody [NB200-106] - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.
Immunocytochemistry/Immunofluorescence: p19ARF Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.
Immunohistochemistry-Paraffin: p19ARF Antibody [NB200-106] - IHC analysis of formalin fixed paraffin-embedded (FFPE) mouse spleen using p19ARF antibody at 10 ug/ml on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining in the germinal centers of spleen was observed. Staining was performed by Histowiz.
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AKT [p Ser473]
Antibody - Pan Specif
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Akt phosphorylated at S473 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Ezrin Antibody
NBP2-16396
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c-Myc Antibody
NB600-335
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TAS2R50
Overexpression Lysate
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Western Blot: LPP Antibody [NBP1-89549] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/Immunofluorescence: LPP Antibody [NBP1-89549] - Staining of human cell line U-251 MG shows localization to cytosol and focal adhesion sites. Antibody staining is shown in green.
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Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-25959B
DyLight 350 Labeled NBP1-25959UV
DyLight 405 Labeled NBP1-25959V
DyLight 488 Labeled NBP1-25959G
DyLight 550 Labeled NBP1-25959R
DyLight 650 Labeled NBP1-25959C
DyLight 755 Labeled NBP1-25959IR
FITC Labeled NBP1-25959F
HRP Labeled NBP1-25959H
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol PALLD
Entrez