Home » Palladin » Palladin Antibodies » Palladin Antibody (1E6) [DyLight 680]

Palladin Antibody (1E6) [DyLight 680]

 Submit a Review

Images

 
There are currently no images for Palladin Antibody (NBP1-25959FR).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
1E6
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 680

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP1-25959AF405
Alexa Fluor 488
NBP1-25959AF488
Alexa Fluor 647
NBP1-25959AF647
Alexa Fluor 700
NBP1-25959AF700
Biotin
NBP1-25959B
DyLight 350
NBP1-25959UV
DyLight 405
NBP1-25959V
DyLight 488
NBP1-25959G
DyLight 550
NBP1-25959R
DyLight 650
NBP1-25959C
DyLight 755
NBP1-25959IR
FITC
NBP1-25959F
HRP
NBP1-25959H
Unconjugated
NBP1-25959
100% Guarantee on every product

Palladin Antibody (1E6) [DyLight 680] Summary

Immunogen
Endogenous Palladin immunoprecipitated from chicken embryo fibroblasts.
Localization
Cytoplasm, cytoskeleton. Note: Localizes to stress fibers and Z-disks. Cell junction, focal adhesion. Cell projection, ruffle. Cell projection, lamellipodium.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PALLD
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This Palladin (1E6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB a band is seen at ~85 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken, Human, Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Palladin Antibody (1E6) [DyLight 680]

  • CGI151
  • CGI-151
  • FLJ22190
  • FLJ38193
  • KIAA0992FLJ39139
  • MYN
  • myoneurin
  • Palladin
  • palladin, cytoskeletal associated protein
  • PALLD
  • PNCA1
  • Sarcoma antigen NY-SAR-77
  • SIH002
  • SIH002FLJ61376

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Myotilin Antibody
NBP1-87851
Western Blot: Myotilin Antibody [NBP1-87851] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: Myotilin Antibody [NBP1-87851] - Staining of human skin shows low expression as expected.
Immunohistochemistry-Paraffin: Myotilin Antibody [NBP1-87851] - Staining of human skeletal muscle shows high expression.
Species: Hu
Applications: WB, IHC, IHC-P
VASP Antibody
NBP1-85792
Western Blot: VASP Antibody [NBP1-85792] - Analysis in human cell line HEL.
Immunocytochemistry/Immunofluorescence: VASP Antibody [NBP1-85792] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: VASP Antibody [NBP1-85792] - Staining in human small intestine and skeletal muscle tissues using anti-VASP antibody. Corresponding VASP RNA-seq data are presented for the same tissues.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MYPN Antibody
NBP2-38924
Immunohistochemistry-Paraffin: MYPN Antibody [NBP2-38924] - Staining of human skeletal muscle shows high expression.
Immunohistochemistry-Paraffin: MYPN Antibody [NBP2-38924] - Staining of human prostate shows low expression as expected.
Immunohistochemistry-Paraffin: MYPN Antibody [NBP2-38924] - Staining in human skeletal muscle and prostate tissues using anti-MYPN antibody. Corresponding MYPN RNA-seq data are presented for the same tissues.
Species: Hu
Applications: IHC, IHC-P
p19ARF Antibody
NB200-106
Western Blot: p19ARF Antibody [NB200-106] - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.
Immunocytochemistry/Immunofluorescence: p19ARF Antibody [NB200-106] - p19ARF antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: p19ARF Antibody [NB200-106] - IHC analysis of formalin fixed paraffin-embedded (FFPE) mouse spleen using p19ARF antibody at 10 ug/ml on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining in the germinal centers of spleen was observed. Staining was performed by Histowiz.
(40 Publications)
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
AKT [p Ser473]
Antibody - Pan Specif
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Akt phosphorylated at S473 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
(18 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
Ezrin Antibody
NBP2-16396
Western Blot: Ezrin Antibody [NBP2-16396] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/Immunofluorescence: Ezrin Antibody [NBP2-16396] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Ezrin protein stained by Ezrin antibody [N2C2], Internal diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:1000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - araffin-embedded rat duodenum. Ezrin antibody [N2C2], Internal diluted at 1:500.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
c-Myc Antibody
NB600-335
Western Blot: c-Myc Antibody [NB600-335] - Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-c-myc antibody NB600-335 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-335] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-c-Myc conjugated to Alexa Fluor 488 [NB600-335AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.
Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ug/ml (1:25,000).
(1 Review)
(38 Publications)
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
BRCA2 Antibody
(234403)
MAB2476
Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human BRCA2 Monoclonal Antibody (Catalog # MAB2476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BRCA2 at approximately 380 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(5 Publications)
Species: Hu
Applications: WB, IHC
TAS2R50
Overexpression Lysate
NBP2-05027
Western Blot: TAS2R50 Overexpression Lysate (Adult Normal) [NBP2-05027] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TAS2R50.
Species: Hu
ROS Antibody (4-6G)
NBP2-50179
ROS Antibody (4-6G)
Species: Hu
Applications: WB, Flow, ICC/IF, IP, CyTOF-ready
OR13G1 RNAi
H00441933-R02
Species: Hu
PDLIM1 Antibody
H00009124-D01P
Western Blot: PDLIM1 Antibody [H00009124-D01P] - Analysis of PDLIM1 expression in human pancreas.
Western Blot: PDLIM1 Antibody [H00009124-D01P] - Analysis of PDLIM1 expression in HepG2.
Western Blot: PDLIM1 Antibody [H00009124-D01P] - Analysis of PDLIM1 expression in human liver.
(1 Publication)
Species: Hu
Applications: WB, ELISA
ANKRD1 Antibody
NBP2-14292
Immunocytochemistry/Immunofluorescence: ANKRD1 Antibody [NBP2-14292] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.
Immunohistochemistry-Paraffin: ANKRD1 Antibody [NBP2-14292] - Staining in human heart muscle and testis tissues. Corresponding ANKRD1 RNA-seq data are presented for the same tissues.
Immunohistochemistry: ANKRD1 Antibody [NBP2-14292] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
BRUNOL4 Antibody
NBP1-89932
Immunocytochemistry/Immunofluorescence: BRUNOL4 Antibody [NBP1-89932] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
Immunohistochemistry-Paraffin: BRUNOL4 Antibody [NBP1-89932] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Max Antibody
[Unconjugated]
AF4304
Western blot shows lysates of LNCaP human prostate cancer cell line and PC-3 human prostate cancer cell line. Gels were loaded with 20 μg of cytoplasmic (Cyto) and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human Max Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4304) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Max was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Publication)
Species: Hu
Applications: WB
LPP Antibody
NBP1-89549
Western Blot: LPP Antibody [NBP1-89549] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/Immunofluorescence: LPP Antibody [NBP1-89549] - Staining of human cell line U-251 MG shows localization to cytosol and focal adhesion sites.
Immunohistochemistry-Paraffin: LPP Antibody [NBP1-89549] - Staining in human smooth muscle and pancreas tissues using anti-LPP antibody. Corresponding LPP RNA-seq data are presented for the same tissues.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.
(1 Review)
(13 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
Dysbindin Antibody
NBP1-85299
Western Blot: Dysbindin Antibody [NBP1-85299] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: Dysbindin Antibody [NBP1-85299] - Immunofluorescent staining of human cell line A-431 shows localization to microtubules.
Immunohistochemistry-Paraffin: Dysbindin Antibody [NBP1-85299] - Staining in human lymph node and pancreas tissues using anti-DTNBP1 antibody. Corresponding DTNBP1 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Palladin Antibody (NBP1-25959FR) (0)

There are no publications for Palladin Antibody (NBP1-25959FR).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Palladin Antibody (NBP1-25959FR) (0)

There are no reviews for Palladin Antibody (NBP1-25959FR). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Palladin Antibody (NBP1-25959FR) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-25959B
DyLight 350 Labeled NBP1-25959UV
DyLight 405 Labeled NBP1-25959V
DyLight 488 Labeled NBP1-25959G
DyLight 550 Labeled NBP1-25959R
DyLight 650 Labeled NBP1-25959C
DyLight 755 Labeled NBP1-25959IR
FITC Labeled NBP1-25959F
HRP Labeled NBP1-25959H
Learn about Custom Antibody Labeling

Additional Palladin Products

Bioinformatics Tool for Palladin Antibody (NBP1-25959FR)

Discover related pathways, diseases and genes to Palladin Antibody (NBP1-25959FR). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Palladin Antibody (NBP1-25959FR)

Discover more about diseases related to Palladin Antibody (NBP1-25959FR).
 

Pathways for Palladin Antibody (NBP1-25959FR)

View related products by pathway.

Research Areas for Palladin Antibody (NBP1-25959FR)

Find related products by research area.

Blogs on Palladin

There are no specific blogs for Palladin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Palladin Antibody (1E6) [DyLight 488]
NBP1-25959G
12.5% SDS-PAGE Stained with Coomassie Blue.
Recombinant Human Palladin Protein
H00023022-Q01-10ug
Western Blot: TAS2R50 Overexpression Lysate (Adult Normal) [NBP2-05027] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TAS2R50.
TAS2R50 Overexpression Lysate (Native)
NBP2-05027
Other Palladin RNAi
Palladin RNAi
H00023022-R04-20nmol

Contact Information

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Palladin Antibody (1E6) [DyLight 680] and receive a gift card or discount.


Bioinformatics

Gene Symbol PALLD
Entrez