Mitofusin 1 Antibody (11E9-1H12)

Images

 
Genetic Strategies: Western Blot: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Analysis of Mitofusin-1 expression in (1) MEF wild-type and (2) MEF Mitofusin-1-null whole cell lysates using NBP1-71775.
Immunocytochemistry/ Immunofluorescence: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Antibody was tested in 3T3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Analysis of Mitofusin-1 in mouse liver.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KO
Clone
11E9-1H12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

Mitofusin 1 Antibody (11E9-1H12) Summary

Immunogen
Mouse Mitofusin 1 [Swiss-Prot: Q811U4]
Localization
Mitochondrion outer membrane; Multi-pass membrane protein
Marker
Mitochondrial Fusion Marker
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MFN1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Knockout Validated
Application Notes
In Western blot, a band can be seen at ~80 kDa.
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-71775 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23658809). Human reactivity reported in scientific literature (PMID: 24710686, 24928681). Knockout-validation on mouse MEF cells.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Mitofusin 1 Antibody (11E9-1H12)

  • DKFZp762F247
  • EC 3.6.5
  • EC 3.6.5.-
  • FLJ20693
  • Fzo homolog
  • hfzo1
  • hfzo2
  • MFN1
  • MGC41806
  • mitochondrial transmembrane GTPase Fzo-1
  • mitochondrial transmembrane GTPase FZO-2
  • Mitofusin 1
  • mitofusin-1
  • putative transmembrane GTPase
  • Transmembrane GTPase MFN1

Background

Mitochondria are dynamic organelles that continuously divide and fuse, and their dynamics/distribution is critical for their role in bioenergetics and cell survival. Mitochondrial fusion process is mediated by mitofusin 1 (MFN1) and mitofusin 2 (MFN2) proteins in the outer membrane as well as OPA1 in the inner mitochondrial membrane. MFN1 is an essential transmembrane GTPase critical to mitochondrial fusion; acts independently of the cytoskeleton, and its overexpression induces formation of mitochondrial networks. MFN1 forms homo-/-heteromultimers (with MFN2), and the multimerization has been proposed to be essential for mitochondrion fusion. MFN1 interacts with VAT1 and is ubiquitinated by MARCH5. A mutation in the GTPase domain disrupts the mitochondrial fusion activity of MFN1/MFN2 and loss of MFN1/2 leads to mitochondrial dysfunction mediated embryonic lethality in mice. MFN proteins have been reported to regulate mitochondrial membrane potential, while silencing of MFN1/2 resulte in mitochondrial fragmentation and increased apoptotic sensitivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Mitofusin 1 Antibody (NBP1-71775)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Flow, ICC/IF, WB.


Filter By Application
Flow
(1)
ICC/IF
(1)
WB
(12)
All Applications
Filter By Species
Human
(2)
Mouse
(7)
Rat
(3)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NBP1-71775 Applications Species
Halder A, Yadav K, Aggarwal A et al. Activation of TNFR1 and TLR4 following oxygen glucose deprivation promotes mitochondrial fission in C6 astroglial cells Cell. Signal. Jul 18 2020 [PMID: 32693013] (Flow, WB, ICC/IF, Rat) Flow, WB, ICC/IF Rat
Zhang X, Zhao D, Wu W et al. Melatonin regulates mitochondrial dynamics and alleviates neuron damage in prion diseases Aging (Albany NY) Jun 10 2020 [PMID: 32526704]
Li T, Li K, Zhang S et al. Overexpression of apoptosis inducing factor aggravates hypoxic-ischemic brain injury in neonatal mice Cell Death Dis Jan 30 2020 [PMID: 32001673] (WB, Mouse) WB Mouse
Kim D, Cho J, Kang H Protective Effect of Exercise Training against the Progression of Alzheimer's Disease in 3xTg-AD Mice Behav. Brain Res. Jul 17 2019 [PMID: 31325514] (WB, Mouse) WB Mouse
Bahat A, Goldman A, Zaltsman Y et al. MTCH2-mediated mitochondrial fusion drives exit from naive pluripotency in embryonic stem cells Nat Commun Dec 3 2018 [PMID: 30510213] (WB, Mouse) WB Mouse
Nobis S, Goichon A, Achamrah N et al. Alterations of proteome, mitochondrial dynamic and autophagy in the hypothalamus during activity-based anorexia Sci Rep May 8 2018 [PMID: 29740148] (WB, Mouse) WB Mouse
Montaigne D, Marechal X, Coisne A et al. Myocardial Contractile Dysfunction is Associated with Impaired Mitochondrial Function and Dynamics in Type 2 Diabetic but not in Obese Patients. Circulation. 2014 Jun 13 [PMID: 24928681] (WB, Human) WB Human
Wu Z, Zhu Y, Cao X et al. Mitochondrial Toxic Effects of Abeta Through Mitofusins in the Early Pathogenesis of Alzheimer's Disease. Mol. Neurobiol. 2014 Apr 08 [PMID: 24710686] (WB, Human) WB Human
Amadoro G, Corsetti V, Florenzano F et al. AD-linked, toxic NH2 human tau affects the quality control of mitochondria in neurons. Neurobiol. Dis. 2014 Feb 1 [PMID: 24411077] (WB, Rat) WB Rat
Wappler EA, Institoris A, Dutta S et al. Mitochondrial Dynamics Associated with Oxygen-Glucose Deprivation in Rat Primary Neuronal Cultures. PLoS One 2013 May 2 [PMID: 23658809] (WB, Rat) WB Rat
Show All 13 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Mitofusin 1 Antibody (NBP1-71775). (Showing 1 - 1 of 1 FAQs).

  1. Could you please tell me if you'd offer the mitofusin 1 antibody Cat# NBP1-71775 for using it with rat tissue? If not, could you please offer one that works with rats?
    • NBP1-71775 has been validated for use in human and mouse only. Rat is not one of the listed species and we cannot guarantee that it will work in rat. Unfortunately, we currently do not have a Mitofusin-1 antibody that has been tested and validated for use in rat. If you'd be interested in testing one of our antibodies, we can recommend our Innovator's Reward program. Our Innovator's Reward is offered to reward researchers for testing new species and applications with our products. If testing on this antibody is performed on an untested species and/or application, and the results are shared, then the antibody is eligible for the nnovator's Reward. Under this program, Novus will provide you a credit of equal or lesser value on a future product of equal value in exchange for your new data in the form of an online review. The great thing is you are eligible even if the antibody doesn't work because the data is still useful to us. (Note: Rat was added as a species on the datasheet from a researcher who published using rat in May 2013 - please see PMID 23658809 for details).

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-71775AF350
Alexa Fluor 405 NBP1-71775AF405
Alexa Fluor 488 NBP1-71775AF488
Alexa Fluor 532 NBP1-71775AF532
Alexa Fluor 594 NBP1-71775AF594
Alexa Fluor 647 NBP1-71775AF647
Alexa Fluor 700 NBP1-71775AF700
Alexa Fluor 750 NBP1-71775AF750
Biotin NBP1-71775B
DyLight 350 NBP1-71775UV
DyLight 405 NBP1-71775V
DyLight 488 NBP1-71775G
DyLight 550 NBP1-71775R
DyLight 594 NBP1-71775DL594
DyLight 650 NBP1-71775C
DyLight 680 NBP1-71775FR
DyLight 755 NBP1-71775IR
FITC NBP1-71775F
HRP NBP1-71775H
Janelia Fluor 549 NBP1-71775JF549
Janelia Fluor 646 NBP1-71775JF646

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Bioinformatics

Gene Symbol MFN1
Entrez