NF-H Antibody

Images

 
Western Blot: NF-H Antibody [NB300-217] - Analysis of spinal cord lysates from different species using chicken pAb to NF-H, NB300-217, dilution 1:20,000 in green: [1] protein standard (red), [2] rat, [3] mouse, and [4] ...read more
Immunocytochemistry/ Immunofluorescence: NF-H Antibody [NB300-217] - Imaging of Feline optic nerve at antibody dilution 1:5000. This image was submitted via customer Review.
Immunohistochemistry Free-Floating: NF-H Antibody [NB300-217] - Analysis of a rat cerebellum section stained with NF-H antibody, dilution 1:5,000 (Red), and costained with rabbit GFAP pAb, dilution 1:5,000 Green). DAPI ...read more
Western Blot: NF-H Antibody [NB300-217] - Analysis of 200kDa Neurofilament Heavy expression in rat spinal cord extract. The first lane is Coomassie Brilliant Blue stained and the second lane is probed with chicken ...read more
Immunocytochemistry/ Immunofluorescence: NF-H Antibody [NB300-217] - Rat neurons stained with 200kDa Neurofilament Heavy Antibody NB300-217.
Immunocytochemistry/ Immunofluorescence: NF-H Antibody [NB300-217] - SH-SY5Y cells stained with 200kDa Neurofilament Heavy Antibody NB300-217 (red) and Fibrillarin Antibody NB300-269 (green). Nuclear DNA is stained with ...read more
Immunocytochemistry/ Immunofluorescence: NF-H Antibody [NB300-217] - Mixed rat neuron/glial cultures stained with Neurofilament Light (NF-L) antibody NBP1-05217 [green] and Neurofilament Heavy (NF-H) antibody NB300-217 ...read more
Immunohistochemistry-Frozen: NF-H Antibody [NB300-217] - Canine optic nerve, antibody specifically labelled the axons (Green). Blue is DAPI. Image taken with an epifluorescent microscope and was incubated at 1:10000 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Eq, FeSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

NF-H Antibody Summary

Immunogen
Native NF-H (phosphorylated) purified from bovine spinal cord.
Marker
Neuronal Marker
Specificity
Reacts very strongly with NF-H KSP type phosphorylated repeats. Reactivity with non-phosphorylated KSP sequences is orders of magnitude weaker.
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
NEFH
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100000
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:20000
  • Immunohistochemistry 1:20000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunohistochemistry Free-Floating 1:5000
Application Notes
In WB a band can be seen at around 200kDa corresponding to rodent Neurofilament Heavy. In larger species such as porcine or human, Neurofilament Heavy runs a little slower and can be seen around 210-220kDa. Use in IHC-P and IHC-Fr reported in scientific literature (PMID: 31054993 and 26319770 respectively).
Theoretical MW
200-220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NF-H Knockout HeLa Cell Lysate
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB300-217 in the following applications:

Publications
Read Publications using
NB300-217 in the following applications:

Reactivity Notes

Feline reactivity reported in verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
5mM Sodium Azide
Purity
Ammonium sulfate precipitation

Alternate Names for NF-H Antibody

  • 200 kDa neurofilament protein
  • KIAA0845
  • NEFH
  • Neurofilament Heavy (200kDa)
  • neurofilament heavy polypeptide
  • Neurofilament triplet H protein
  • neurofilament, heavy polypeptide 200kDa
  • neurofilament, heavy polypeptide
  • NF200
  • NFH
  • NF-H

Background

Neurofilaments can be defined as the intermediate or 10nm filaments found specifically in neuronal cells. Neurofilaments appears as fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid axon impulse conduction. They are composed of a mixture of subunits which usually include the three neurofilament triplet proteins, known as Neurofilament Light (NF-L), Neurofilament Medium (NF-M) and Neurofilament Heavy (NF-H). Neurofilaments may also include smaller amounts of peripherin, a-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-109
Species: Hu, Mu, Rt, Dr, I, Ma
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NB300-217
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl

Publications for NF-H Antibody (NB300-217)(3)

Reviews for NF-H Antibody (NB300-217) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Canine, Feline.

Reviews using NB300-217:
Filter by Applications
IHC-Fr
(1)
ICC
(1)
All Applications
Filter by Species
Canine
(1)
Feline
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen NF-H NB300-217
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reviewed by:
Anonymous
IHC-Fr Canine 02/06/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedOptic nerve
SpeciesCanine
Lot7575-2

Comments

CommentsNB300-217 was incubated at 1:10000 for 1hr at RT.
Immunocytochemistry NF-H NB300-217
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reviewed by:
Anonymous
ICC Feline 05/10/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedoptic nerve
SpeciesFeline
Lot2796-6

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NF-H Antibody (NB300-217). (Showing 1 - 1 of 1 FAQs).

  1. What is the concentration of this product?
    • For NB300-217 Lot: 27967, the concentration is 22.5mg/ml (Total IgY prep).

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
02/06/2019
Application: IHC-Fr
Species: Canine

Anonymous
05/10/2017
Application: ICC
Species: Feline

Bioinformatics

Gene Symbol NEFH
Entrez
Uniprot