Mitofusin 1 Antibody (11E9-1H12) [FITC]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, KO
• Clone: 11E9-1H12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

Mitofusin 1 Antibody (11E9-1H12) [FITC] Summary

• Immunogen: Mouse Mitofusin 1 [Swiss-Prot: Q811U4]
• Localization: Mitochondrion outer membrane; Multi-pass membrane protein
• Marker: Mitochondrial Fusion Marker
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MFN1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23658809). Human reactivity reported in scientific literature (PMID: 24710686, 24928681).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Mitofusin 1 Antibody (11E9-1H12) [FITC]

• DKFZp762F247
• EC 3.6.5
• EC 3.6.5.-
• FLJ20693
• Fzo homolog
• hfzo1
• hfzo2
• MFN1
• MGC41806
• mitochondrial transmembrane GTPase Fzo-1
• mitochondrial transmembrane GTPase FZO-2
• Mitofusin 1
• mitofusin-1
• putative transmembrane GTPase
• Transmembrane GTPase MFN1

Background

Mitochondria are dynamic organelles that continuously divide and fuse, and their dynamics/distribution is critical for their role in bioenergetics and cell survival. Mitochondrial fusion process is mediated by mitofusin 1 (MFN1) and mitofusin 2 (MFN2) proteins in the outer membrane as well as OPA1 in the inner mitochondrial membrane. MFN1 is an essential transmembrane GTPase critical to mitochondrial fusion; acts independently of the cytoskeleton, and its overexpression induces formation of mitochondrial networks. MFN1 forms homo-/-heteromultimers (with MFN2), and the multimerization has been proposed to be essential for mitochondrion fusion. MFN1 interacts with VAT1 and is ubiquitinated by MARCH5. A mutation in the GTPase domain disrupts the mitochondrial fusion activity of MFN1/MFN2 and loss of MFN1/2 leads to mitochondrial dysfunction mediated embryonic lethality in mice. MFN proteins have been reported to regulate mitochondrial membrane potential, while silencing of MFN1/2 resulte in mitochondrial fragmentation and increased apoptotic sensitivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MFN1