Kif2a Antibody

Knockout Validated: Kif2a Antibody [NB500-180] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Kif2a knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:10000 of Rabbit Anti-Human Kif2a Polyclonal Antibody (Catalog # NB500-180) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Kif2a at approximately 100 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.

Western Blot: Kif2a Antibody [NB500-180] - Analysis of Kif2a in Hela, 3T3 and PC12 lysate.

Immunocytochemistry/Immunofluorescence: Kif2a Antibody [NB500-180] - Kif2a antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Simple Western: Kif2a Antibody [NB500-180] - Lane view shows a specific band for Kif2a in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Po, Ma
• Applications: WB, Simple Western, ICC/IF, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Kif2a Antibody Summary

• Immunogen: A recombinant segment of the N-terminal domain of human Kif2a. [UniProt# O00139]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KIF2A
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:10000
  • Immunoprecipitation 1:10-1:500
  • Knockout Validated
  • Simple Western 1:1000
  • Western Blot 1:10000
• Application Notes: This Kif2a antibody is useful for Western blot, Immunoprecipitation, and Immunocytochemistry/Immunofluorescence. A band at approx. 110 kDa can be detected by Western blot.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for Kif2a Antibody

• HK2
• KIF2kinesin-2
• kinesin heavy chain member 2
• kinesin heavy chain member 2A
• Kinesin, heavy chain, 2
• Kinesin-2
• kinesin-like protein KIF2A
• KNS2

Background

Kif2a (Kinesin-like protein KIF2A or Kinesin-2) belongs to Kinesin-13 family that includes Kif2a, Kif2b and Kif2c members which are classified as microtubule depolymerases with potential of depolymerizing microtubules at their ends. Among these proteins, Kif2a specifically localizes to centrosomes during mitotic cell division and is essential to normal congress of chromosomes at the metaphase plate, normal spindle dynamics/turnover and for the formation of bipolar mitotic spindles. Kif2a gets localized to the spindle microtubules and spindle poles from prophase to metaphase. Kif2a-PSRC1 interactions leads to its recruitment to mitotic spindles, PLK1 kiase activity is required for Kif2a targeting to spindle microtubules and spindle poles. siRNA knockdown of Kif2a in human cancer cells or Kif2a blocking with antibody addition to Xenopus eggs leads to monopolar spindles formation and cell cycle block suggesting that Kif2a is essential for bipolar spindle assembly and normal progression of mitosis. Kif2a has been shown to be essential for proper neuronal outgrowth and its depletion caused neuronal migratory delay by changing microtubule dynamics around the cell body suggesting that Kif2a is implicated in non-mitotic cells also. Moreover, Kif2a has been established as a plus end-directed microtubule-dependent motor essential for normal brain development and that it regulates microtubule dynamics during axonal growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kif2a Antibody (NB500-180)(17)

Publications using NB500-180

• Shankar S, Hsu ZT, Ezquerra A et al. Α gamma -tubulin complex-dependent pathway suppresses ciliogenesis by promoting cilia disassembly Cell reports 2022-11-15 [PMID: 36384111] (WB, Human)
• Tay A, Melosh N Mechanical Stimulation after Centrifuge-Free Nano-Electroporative Transfection Is Efficient and Maintains Long-Term T Cell Functionalities Small (Weinheim an der Bergstrasse, Germany) 2021-08-15 [PMID: 34396686]
• Steblyanko, Y, Rajendraprasad, G Et al. Microtubule poleward flux in human cells is driven by the coordinated action of four kinesins. EMBO J 2020-12-01 [PMID: 33073400] (IHC, Drosophila melanogaster)
• Gwon D, Hong J et al. c-Cbl Acts as an E3 Ligase Against DDA3 for Spindle Dynamics and Centriole Duplication during Mitosis. Mol Cells 2019-12-31 [PMID: 31722512] (ICC/IF, WB, Human)
• Miller KE, Session AM, Heald R Kif2a Scales Meiotic Spindle Size in Hymenochirus boettgeri Curr. Biol. 2019-09-23 [PMID: 31630945]
• So C, Seres K. B, et al. A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. Science 2019-06-28 [PMID: 31249032] (ICC/IF, Bovine, Mouse, Porcine, Sheep)
• Chen MH, Liu Y, Wang YL et al. KIF2A regulates the spindle assembly and the metaphase I-anaphase I transition in mouse oocyte. Sci Rep. 2016-12-19 [PMID: 27991556] (ICC/IF, Mouse)
• Yi ZY, Ma XS, Liang QX et al. Kif2a regulates spindle organization and cell cycle progression in meiotic oocytes. Sci Rep. 2016-12-19 [PMID: 27991495] (ICC/IF, WB, Mouse)
• Bendre S, Rondelet A, Hall C et al. GTSE1 tunes microtubule stability for chromosome alignment and segregation by inhibiting the microtubule depolymerase MCAK. J. Cell Biol. 2016-12-05 [PMID: 27881713] (Human)
• Uematsu K, Okumura F, Tonogai S et al. ASB7 regulates spindle dynamics and genome integrity by targeting DDA3 for proteasomal degradation. J. Cell Biol. 2016-10-10 [PMID: 27697924] (WB, Human)
• Kwon HJ, Park JE, Song H, Jang CY. DDA3 and Mdp3 modulate Kif2a recruitment onto the mitotic spindle to control minus-end spindle dynamics. J. Cell. Sci. 2016-06-09 [PMID: 27284004] (ICC/IF)
• Park JE, Song H, Kwon HJ et al. Ska1 cooperates with DDA3 for spindle dynamics and spindle attachment to kinetochore. Biochem. Biophys. Res. Commun. 2016-01-19 [PMID: 26797278] (ICC/IF, WB, Human)
• Zaganjor E, Weil LM, Gonzales JX et al. Ras transformation uncouples the kinesin-coordinated cellular nutrient response. Proc. Natl. Acad. Sci. U.S.A. 2014-07-22 [PMID: 25002494] (WB, Human)
  
  Details:
  Kif2a antibody used for WB on HBEC3KT cells treated with control KIF2C or KIF2A siRNA, starved, and then stimulated with amino acids/AA or fresh medium with serum/M (Fig 1C). Antibody also used for WB in lysates of HBEC3KT and HCT116 cells (Fig. 3C).
• Ma N, Tulu US, Ferenz NP et al. Poleward transport of TPX2 in the mammalian mitotic spindle requires dynein, Eg5, and microtubule flux Mol Biol Cell 2010-03-15 [PMID: 20110350] (ICC/IF, Porcine)
• Jang C-Y, Coppinger JA, Seki A et al. Plk1 and Aurora A regulate the depolymerase activity and the cellular localization of Kif2a. J Cell Sci;122(Pt 9):1334-41. 2009-05-01 [PMID: 19351716]
• Jang CY, Wong J, Coppinger JA et al. DDA3 recruits microtubule depolymerase kif2a to spindle poles and controls spindle dynamics and mitotic chromosome movement. J Cell Biol 2008-04-01 [PMID: 18411309] (ICC/IF, WB, Human)
• Ferenz NP, Wadsworth P. Prophase microtubule arrays undergo flux-like behavior in mammalian cells. Mol Biol Cell;18(10):3993-4002. 2007-10-01 [PMID: 17671163]

FAQs for Kif2a Antibody (NB500-180). (Showing 1 - 2 of 2 FAQs).

1. Can you provide the immunogen sequence for the Kif2a Antibody Cat. No. NB500-180? If considered proprietary, can you provide the % homology with drosophila?
   • The exact immunogen sequence of NB500-180 is proprietary, but it falls within amino acids 1-120 of human Kif2A (Accession #: O00139). Based on an alignment of the immunogen with the Drosophila melanogaster sequence, I am getting a homology of about 25%.
2. We receive an enquiry about the following antibody: Anti-Kif2a antibody (NB 500-180). The customer would like to know the immunogen of the antibody. If this information is not available, I would appreciate if you could at least give the amino acids range at the N-ter recognized by the antibody.
   • Unfortunately many of our immunogen sequences are proprietary and we are unable to disclose the exact amino acid range. However, we can confirm you that the immunogen which was used to generate our Kif2a antibody with catalogue number NB500-180 is derived from a region within the first 150 amino acids of the protein.

Bioinformatics

• Gene Symbol: KIF2A
• Entrez:
  • 3796(Human)
  • 16563(Mouse)
  • 84391(Rat)
• Uniprot:
  • O00139(Human)