Kif2a Antibody

Images

 
Western Blot: Kif2a Antibody [NB500-180] - Analysis of Kif2a in Hela, 3T3 and PC12 lysate.
Immunocytochemistry/ Immunofluorescence: Kif2a Antibody [NB500-180] - Kif2a antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Simple Western: Kif2a Antibody [NB500-180] - Lane view shows a specific band for Kif2a in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, MaSpecies Glossary
Applications WB, Simple Western, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Kif2a Antibody Summary

Immunogen
A recombinant segment of the N-terminal domain of human Kif2a. [UniProt# O00139]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KIF2A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Simple Western 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10000
  • Immunoprecipitation 1:10-1:500
Application Notes
This Kif2a antibody is useful for Western blot, Immunoprecipitation, and Immunocytochemistry/Immunofluorescence. A band at approx. 110 kDa can be detected by Western blot.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Kif2a Lysate (NBP2-65247)
Publications
Read Publications using
NB500-180 in the following applications:

  • 6 publications
  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for Kif2a Antibody

  • HK2
  • KIF2kinesin-2
  • kinesin heavy chain member 2
  • kinesin heavy chain member 2A
  • Kinesin, heavy chain, 2
  • Kinesin-2
  • kinesin-like protein KIF2A
  • KNS2

Background

Kif2a (Kinesin-like protein KIF2A or Kinesin-2) belongs to Kinesin-13 family that includes Kif2a, Kif2b and Kif2c members which are classified as microtubule depolymerases with potential of depolymerizing microtubules at their ends. Among these proteins, Kif2a specifically localizes to centrosomes during mitotic cell division and is essential to normal congress of chromosomes at the metaphase plate, normal spindle dynamics/turnover and for the formation of bipolar mitotic spindles. Kif2a gets localized to the spindle microtubules and spindle poles from prophase to metaphase. Kif2a-PSRC1 interactions leads to its recruitment to mitotic spindles, PLK1 kiase activity is required for Kif2a targeting to spindle microtubules and spindle poles. siRNA knockdown of Kif2a in human cancer cells or Kif2a blocking with antibody addition to Xenopus eggs leads to monopolar spindles formation and cell cycle block suggesting that Kif2a is essential for bipolar spindle assembly and normal progression of mitosis. Kif2a has been shown to be essential for proper neuronal outgrowth and its depletion caused neuronal migratory delay by changing microtubule dynamics around the cell body suggesting that Kif2a is implicated in non-mitotic cells also. Moreover, Kif2a has been established as a plus end-directed microtubule-dependent motor essential for normal brain development and that it regulates microtubule dynamics during axonal growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB500-180
Species: Hu, Mu, Rt, Po, Ma
Applications: WB, Simple Western, ICC/IF, IP

Publications for Kif2a Antibody (NB500-180)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(6)
WB
(5)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB500-180 Applications Species
Chen MH, Liu Y, Wang YL et al. KIF2A regulates the spindle assembly and the metaphase I-anaphase I transition in mouse oocyte. Sci Rep. Dec 19 2016 [PMID: 27991556] (ICC/IF, Mouse) ICC/IF Mouse
Yi ZY, Ma XS, Liang QX et al. Kif2a regulates spindle organization and cell cycle progression in meiotic oocytes. Sci Rep. Dec 19 2016 [PMID: 27991495] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Bendre S, Rondelet A, Hall C et al. GTSE1 tunes microtubule stability for chromosome alignment and segregation by inhibiting the microtubule depolymerase MCAK. J. Cell Biol. Dec 5 2016 [PMID: 27881713] (Human) Human
Uematsu K, Okumura F, Tonogai S et al. ASB7 regulates spindle dynamics and genome integrity by targeting DDA3 for proteasomal degradation. J. Cell Biol. Oct 10 2016 [PMID: 27697924] (WB, Human) WB Human
Kwon HJ, Park JE, Song H, Jang CY. DDA3 and Mdp3 modulate Kif2a recruitment onto the mitotic spindle to control minus-end spindle dynamics. J. Cell. Sci. Jun 9 2016 [PMID: 27284004] (ICC/IF) ICC/IF
Park JE, Song H, Kwon HJ et al. Ska1 cooperates with DDA3 for spindle dynamics and spindle attachment to kinetochore. Biochem. Biophys. Res. Commun. 2016 Jan 19 [PMID: 26797278] (WB, ICC/IF, Human) WB, ICC/IF Human
Zaganjor E, Weil LM, Gonzales JX et al. Ras transformation uncouples the kinesin-coordinated cellular nutrient response. Proc. Natl. Acad. Sci. U.S.A. 2014 Jul 22 [PMID: 25002494] (WB, Human)

Details:
Kif2a antibody used for WB on HBEC3KT cells treated with control KIF2C or KIF2A siRNA, starved, and then stimulated with amino acids/AA or fresh medium with serum/M (Fig 1C). Antibody also used for WB in lysates of HBEC3KT and HCT116 cells (Fig. 3C).
WB Human
Ma N, Tulu US, Ferenz NP et al. Poleward transport of TPX2 in the mammalian mitotic spindle requires dynein, Eg5, and microtubule flux Mol Biol Cell 2010 Mar 15 [PMID: 20110350] (ICC/IF, Porcine) ICC/IF Porcine
Jang C-Y, Coppinger JA, Seki A et al. Plk1 and Aurora A regulate the depolymerase activity and the cellular localization of Kif2a. J Cell Sci;122(Pt 9):1334-41. 2009 May 1. [PMID: 19351716]
Jang C-Y, Wong J, Coppinger JA, Seki A, Yates JR, III, Fang G. DDA3 recruits microtubule depolymerase Kif2a to spindle poles and controls spindle dynamics and mitotic chromosome movement. J Cell Biol;181(2):255-67. 2008 Apr 21. [PMID: 18411309] (WB, ICC/IF, Human) WB, ICC/IF Human
Show All 11 Publications.

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Product General Protocols

View specific protocols for Kif2a Antibody (NB500-180): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Kif2a Antibody (NB500-180). (Showing 1 - 2 of 2 FAQs).

  1. Can you provide the immunogen sequence for the Kif2a Antibody Cat. No. NB500-180? If considered proprietary, can you provide the % homology with drosophila?
    • The exact immunogen sequence of NB500-180 is proprietary, but it falls within amino acids 1-120 of human Kif2A (Accession #: O00139). Based on an alignment of the immunogen with the Drosophila melanogaster sequence, I am getting a homology of about 25%.
  2. We receive an enquiry about the following antibody: Anti-Kif2a antibody (NB 500-180). The customer would like to know the immunogen of the antibody. If this information is not available, I would appreciate if you could at least give the amino acids range at the N-ter recognized by the antibody.
    • Unfortunately many of our immunogen sequences are proprietary and we are unable to disclose the exact amino acid range. However, we can confirm you that the immunogen which was used to generate our Kif2a antibody with catalogue number NB500-180 is derived from a region within the first 150 amino acids of the protein.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Kif2a.

Kif2a and MT-Destabilization during Mitosis
Kif2a belongs to the Kinesin-13 microtubule depolymerase family that includes members Kif2a, Kif2b, and Kif2c. These proteins are capable of depolymerizing microtubules (MTs) at their ends. During mitotic cell division, Kif2a specifically localizes to...  Read full blog post.

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Bioinformatics

Gene Symbol KIF2A
Entrez
Uniprot