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TGN38 Antibody (2F7.1)

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Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - HeLa and COS7 cells were rinsed with PBS and fixed with -20C methanol for 5 minutes. Cells were incubated with anti-TGN38 antibody diluted ...read more
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Staining of TGN-38 in NRK cells.
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Analysis of Phalloidin (green) and TGN38 (purple) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 ...read more
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Staining in NS-1 Cells.

Product Details

Summary
Reactivity Hu, Mu, Rt, ChHa, Pm, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
2F7.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TGN38 Antibody (2F7.1) Summary

Immunogen
Synthetic peptide corresponding to residues L(18) P S A S K P N N T S S E N N P P(34) C of rat TGN38.
Marker
TGN Marker
Specificity
Detects Trans Golgi Network (TGN) 38 from human and monkey samples and both recombinant and endogenous rat samples.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TGOLN2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20 - 1:50
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:500
Application Notes
IP usage was reported in scientific literature (PMID: 10514494). WB: Detects an approx. 38 kDa protein representing deglycosylated recombinant rat TGN38. Under normal WB conditions, detects an 85-95 kDa band depending on cell type. IF: Staining of TGN38 in NRK cells results in staining predominantly in the TGN. IF: it has been shown that methanol works best as a fixative.
Reviewed Applications
Read 1 Review rated 5
using
NB300-575 in the following applications:

Publications
Read Publications using
NB300-575 in the following applications:

Reactivity Notes

Chinese Hamster reactivity reported in scientific literature (PMID: 11408588). Rabbit reactivity reported in scientific literature (PMID: 10514494). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Use in Human reported in scientific literature (PMID:24576880).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 1 mg/mL BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for TGN38 Antibody (2F7.1)

  • TGN38
  • TGN46
  • TGN46TGN38 homolog
  • TGN48MGC14722
  • TGN51
  • TGN5148, 51kD isoforms)
  • Tgoln1
  • Tgoln2
  • trans-golgi network protein 2
  • Trans-Golgi network protein TGN51
  • Ttgn1

Background

TGN38, also known as TGN46, is localized primarily in the Trans-Golgi network. TGN38 has been reported as being the best available marker for human trans-Golgi network

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, ChHa, Pm, Rb
Applications: WB, Flow, ICC/IF, IHC

Publications for TGN38 Antibody (NB300-575)(17)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC, IHC-P, WB.


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ICC/IF
(7)
IF/IHC
(4)
IHC
(1)
IHC-P
(1)
WB
(1)
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Human
(1)
Mouse
(3)
Rat
(9)
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Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB300-575 Applications Species
Andreska T, Lüningschrör P, Wolf D et al. DRD1 signaling modulates TrkB turnover and BDNF sensitivity in direct pathway striatal medium spiny neurons Cell reports 2023-05-29 [PMID: 37252844] (IHC, Rat) IHC Rat
J Tisdale E, R Artalejo C Rab2 stimulates LC3 lipidation on secretory membranes by noncanonical autophagy Experimental cell research 2023-05-16 [PMID: 37201743]
Maruo K, Nishiyama M, Honda Y et al. Increased GLUT1 expression and localization to Golgi apparatus of acinar cells in the parotid gland of Goto-Kakizaki diabetic rats Archives of oral biology 2022-12-05 [PMID: 36521282] (IHC-P, Rat) IHC-P Rat
Andreska T Effects of dopamine on BDNF/TrkB mediated signaling and plasticity on cortico-striatal synapses Thesis 2021-01-01 (IF/IHC, Rat) IF/IHC Rat
Boyer CK, Bauchle CJ, Zhang J et al. Synchronized proinsulin trafficking reveals delayed Golgi export accompanies ?-cell secretory dysfunction in rodent models of hyperglycemia Scientific reports 2023-03-30 [PMID: 36997560] (ICC/IF, Rat) ICC/IF Rat
Rohli KE, Boyer CK, Bearrows SC et al. ER Redox Homeostasis Regulates Proinsulin Trafficking and Insulin Granule Formation in the Pancreatic Islet beta-Cell Function (Oxford, England) 2022-09-28 [PMID: 36325514] (IF/IHC, Mouse) IF/IHC Mouse
Parchure A, Tian M, Stalder D et al. Liquid-liquid phase separation facilitates the biogenesis of secretory storage granules The Journal of cell biology 2022-12-05 [PMID: 36173346] (ICC/IF, Rat) ICC/IF Rat
Kamat, V;Radtke, JR;Hu, Q;Wang, W;Sweet, IR;Hampe, CS; Autoantibodies directed against glutamate decarboxylase interfere with glucose-stimulated insulin secretion in dispersed rat islets International journal of experimental pathology [PMID: 35246889]
Li M, Shao F, Qian Q Et al. A putative long noncoding RNA-encoded micropeptide maintains cellular homeostasis in pancreatic beta cells Molecular Therapy - Nucleic Acids 2021-12-01 [PMID: 34513312] (ICC/IF, Rat) ICC/IF Rat
Casas M, FadO R, DomInguez JL et al. Sensing of nutrients by CPT1C controls SAC1 activity to regulate AMPA receptor trafficking J. Cell Biol. 2020-10-05 [PMID: 32931550] (ICC/IF, Mouse) ICC/IF Mouse
Show All 17 Publications.

Review for TGN38 Antibody (NB300-575) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB300-575:
Filter by Applications
ICC
(1)
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Filter by Species
Rat
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry TGN38 NB300-575
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5
reviewed by:
Verified Customer
ICC Rat 01/06/2020
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Summary

ApplicationImmunocytochemistry
Sample TestedINS-1
SpeciesRat
LotUB275575

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TGN38 Antibody (NB300-575) (0)

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Secondary Antibodies

 

Isotype Controls

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Verified Customer
01/06/2020
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol TGOLN2
Entrez