TGN38 Antibody (2F7.1)

Immunocytochemistry/Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - HeLa and COS7 cells were rinsed with PBS and fixed with -20C methanol for 5 minutes. Cells were incubated with anti-TGN38 antibody diluted 1:100 in PBS containing 0.2% BSA for 1 hour. The cells were then rinsed in PBS and incubated with goat anti-mouse IgG conjugated to rhodamine for 30 minutes in PBS containing 0.2% BSA. Following washing, cells were imaged using a Zeiss Axioplan microscope. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Staining of TGN-38 in NRK cells.

Immunocytochemistry/Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Analysis of Phalloidin (green) and TGN38 (purple) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a TGN38 monoclonal antibody at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 680 goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin at a dilution of 1:300 (1 unit/mL final concentration) for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.

Immunocytochemistry/Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Staining in NS-1 Cells.

• Reactivity: Hu, Mu, Rt, ChHa, Pm, Rb
• Applications: WB, Flow, ICC/IF, IHC, IHC-P
• Clone: 2F7.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TGN38 Antibody (2F7.1) Summary

• Immunogen: Synthetic peptide corresponding to residues L(18) P S A S K P N N T S S E N N P P(34) C of rat TGN38.
• Marker: TGN Marker
• Specificity: Detects Trans Golgi Network (TGN) 38 from human and monkey samples and both recombinant and endogenous rat samples.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TGOLN2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:20 - 1:50
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:500
• Application Notes: IP usage was reported in scientific literature (PMID: 10514494). WB: Detects an approx. 38 kDa protein representing deglycosylated recombinant rat TGN38. Under normal WB conditions, detects an 85-95 kDa band depending on cell type. IF: Staining of TGN38 in NRK cells results in staining predominantly in the TGN. IF: it has been shown that methanol works best as a fixative.

Reactivity Notes

Chinese Hamster reactivity reported in scientific literature (PMID: 11408588). Rabbit reactivity reported in scientific literature (PMID: 10514494). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Use in Human reported in scientific literature (PMID:24576880).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1 mg/mL BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for TGN38 Antibody (2F7.1)

• TGN38
• TGN46
• TGN46TGN38 homolog
• TGN48MGC14722
• TGN51
• TGN5148, 51kD isoforms)
• Tgoln1
• Tgoln2
• trans-golgi network protein 2
• Trans-Golgi network protein TGN51
• Ttgn1

Background

TGN38, also known as TGN46, is localized primarily in the Trans-Golgi network. TGN38 has been reported as being the best available marker for human trans-Golgi network

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGN38 Antibody (NB300-575)(12)

Publications using NB300-575

• Li M, Shao F, Qian Q Et al. A putative long noncoding RNA-encoded micropeptide maintains cellular homeostasis in pancreatic beta cells Molecular Therapy - Nucleic Acids 2021-12-01 [PMID: 34513312] (ICC/IF, Rat)
• Casas M, FadO R, DomInguez JL et al. Sensing of nutrients by CPT1C controls SAC1 activity to regulate AMPA receptor trafficking J. Cell Biol. 2020-10-05 [PMID: 32931550] (ICC/IF, Mouse)
• Taverner A, MacKay J, Laurent F et al. Cholix protein domain I functions as a carrier element for efficient apical to basal epithelial transcytosis Tissue Barriers 2020-01-13 [PMID: 31928299] (IHC, Rat)
• Bearrows SC, Bauchle CJ, Becker M et al. Chromogranin B regulates early-stage insulin granule trafficking from the Golgi in pancreatic islet beta-cells J. Cell. Sci. 2019-07-01 [PMID: 31182646]
• Tao T, Sun J, Peng Y, et al. Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth J. Biol. Chem. 2019-07-12 [PMID: 31152060] (ICC/IF, Mouse)
• Alsalem J A, Patel D et al. Characterization of vitamin D production by human ocular barrier cells. Invest Ophthalmol Vis Sci 2014-07-04 [PMID: 24576880] (ICC/IF, Human)
• Szmydynger-Chodobska J, Strazielle N, Gandy JR, Keefe TH, Zink BJ, Ghersi-Egea JF, Chodobski A. Posttraumatic invasion of monocytes across the blood-cerebrospinal fluid barrier. J Cereb Blood Flow Metab;32(1):93-104. 2012-01-01 [PMID: 21829211] (WB, Rat)
• Szmydynger-Chodobska J, Fox LM, Lynch KM et al. Vasopressin amplifies the production of proinflammatory mediators in traumatic brain injury. J Neurotrauma 2010-08-01 [PMID: 20504162] (ICC/IF, IHC, Rat)
• Andreska T Effects of dopamine on BDNF/TrkB mediated signaling and plasticity on cortico-striatal synapses Thesis 2021-01-01 (IHC, Rat)
• Rohli KE, Boyer CK, Bearrows SC et al. ER Redox Homeostasis Regulates Proinsulin Trafficking and Insulin Granule Formation in the Pancreatic Islet beta-Cell Function (Oxford, England) 2022-09-28 [PMID: 36325514] (IHC, Mouse)
• Kamat, V;Radtke, JR;Hu, Q;Wang, W;Sweet, IR;Hampe, CS; Autoantibodies directed against glutamate decarboxylase interfere with glucose-stimulated insulin secretion in dispersed rat islets International journal of experimental pathology [PMID: 35246889]
• Mikhaylova M, Reddy PP, Munsch T et al. Calneurons provide a calcium threshold for trans-Golgi network to plasma membrane trafficking. Proc Natl Acad Sci U S A 2009-06-02 [PMID: 19458041]

Review for TGN38 Antibody (NB300-575) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: jerome cattin ICC Rat 01/06/2020

Summary

• Application: Immunocytochemistry
• Sample Tested: INS-1
• Species: Rat
• Lot: UB275575

Bioinformatics

• Gene Symbol: TGOLN2
• Entrez:
  • 10618(Human)
  • 192152(Rat)