Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - HeLa and COS7 cells were rinsed with PBS and fixed with -20C methanol for 5 minutes. Cells were incubated with anti-TGN38 antibody diluted ...read more
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Staining of TGN-38 in NRK cells.
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Analysis of Phalloidin (green) and TGN38 (purple) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 ...read more
This product is unpurified. The exact concentration of antibody is not quantifiable.
In Western blot, this antibody detects an ~38 kDa protein representing deglycosylated recombinant rat TGN38. Under normal Western blot conditions, this antibody detects an 85-95 kDa band depending on cell type.Immunofluorescence staining of TGN38 in NRK cells results in staining predominantly in the TGN.In Immunofluorescence, it has been shown that methanol works best as a fixative.Optimal dilutions should be determined by the end user. Product is useful for Immunoprecipitation as reported in the literature (PMID: 10514494)
Chinese Hamster reactivity reported in scientific literature (PMID: 11408588), Rabbit reactivity reported in scientific literature (PMID: 10514494). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Alternate Names for TGN38 Antibody (2F7.1)
TGN5148, 51kD isoforms)
trans-golgi network protein 2
Trans-Golgi network protein TGN51
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.