TGN38 Antibody (2F7.1)

Images

 
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - HeLa and COS7 cells were rinsed with PBS and fixed with -20C methanol for 5 minutes. Cells were incubated with anti-TGN38 antibody diluted ...read more
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Staining of TGN-38 in NRK cells.
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Analysis of Phalloidin (green) and TGN38 (purple) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 ...read more
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Staining in NS-1 Cells.

Product Details

Summary
Reactivity Hu, Mu, Rt, ChHa, Pm, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
2F7.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TGN38 Antibody (2F7.1) Summary

Immunogen
Synthetic peptide corresponding to residues L(18) P S A S K P N N T S S E N N P P(34) C of rat TGN38.
Marker
TGN Marker
Specificity
Detects Trans Golgi Network (TGN) 38 from human and monkey samples and both recombinant and endogenous rat samples.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TGOLN2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:20 - 1:50
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
Application Notes
IP usage was reported in scientific literature (PMID: 10514494). WB: Detects an approx. 38 kDa protein representing deglycosylated recombinant rat TGN38. Under normal WB conditions, detects an 85-95 kDa band depending on cell type. IF: Staining of TGN38 in NRK cells results in staining predominantly in the TGN. IF: it has been shown that methanol works best as a fixative.
Reviewed Applications
Read 1 Review rated 5
using
NB300-575 in the following applications:

Publications
Read Publications using
NB300-575 in the following applications:

  • 5 publications
  • IHC
    2 publications
  • WB
    1 publication

Reactivity Notes

Chinese Hamster reactivity reported in scientific literature (PMID: 11408588). Rabbit reactivity reported in scientific literature (PMID: 10514494). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Use in Human reported in scientific literature (PMID:24576880).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 1 mg/mL BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for TGN38 Antibody (2F7.1)

  • TGN38
  • TGN46
  • TGN46TGN38 homolog
  • TGN48MGC14722
  • TGN51
  • TGN5148, 51kD isoforms)
  • Tgoln1
  • Tgoln2
  • trans-golgi network protein 2
  • Trans-Golgi network protein TGN51
  • Ttgn1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-575
Species: Hu, Mu, Rt, ChHa, Pm, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P

Publications for TGN38 Antibody (NB300-575)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(5)
IHC
(2)
WB
(1)
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Human
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Mouse
(2)
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(4)
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Showing Publications 1 - 9 of 9.
Publications using NB300-575 Applications Species
Casas M, FadO R, DomInguez JL et al. Sensing of nutrients by CPT1C controls SAC1 activity to regulate AMPA receptor trafficking J. Cell Biol. Oct 5 2020 [PMID: 32931550] (ICC/IF, Mouse) ICC/IF Mouse
Taverner A, MacKay J, Laurent F et al. Cholix protein domain I functions as a carrier element for efficient apical to basal epithelial transcytosis Tissue Barriers Jan 13 2020 [PMID: 31928299] (IHC, Rat) IHC Rat
Bearrows SC, Bauchle CJ, Becker M et al. Chromogranin B regulates early-stage insulin granule trafficking from the Golgi in pancreatic islet beta-cells J. Cell. Sci. Jul 1 2019 [PMID: 31182646]
Tao T, Sun J, Peng Y, et al. Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth J. Biol. Chem. 2019 Jul 12 [PMID: 31152060] (ICC/IF, Mouse) ICC/IF Mouse
Alsalem J A, Patel D et al. Characterization of vitamin D production by human ocular barrier cells. Invest Ophthalmol Vis Sci Jul 4 2014 [PMID: 24576880] (ICC/IF, Human) ICC/IF Human
Li M, Shao F, Qian Q et al. A putative long noncoding RNA-encoded micropeptide maintains cellular homeostasis in pancreatic beta-cells bioRxiv Jan 1 2020 (ICC/IF, Rat) ICC/IF Rat
Szmydynger-Chodobska J, Strazielle N, Gandy JR, Keefe TH, Zink BJ, Ghersi-Egea JF, Chodobski A. Posttraumatic invasion of monocytes across the blood-cerebrospinal fluid barrier. J Cereb Blood Flow Metab;32(1):93-104. 2012 Jan. [PMID: 21829211] (WB, Rat) WB Rat
Szmydynger-Chodobska J, Fox LM, Lynch KM et al. Vasopressin amplifies the production of proinflammatory mediators in traumatic brain injury. J Neurotrauma 2010 Aug [PMID: 20504162] (IHC, ICC/IF, Rat) IHC, ICC/IF Rat
Mikhaylova M, Reddy PP, Munsch T et al. Calneurons provide a calcium threshold for trans-Golgi network to plasma membrane trafficking. PNAS:0903001106. 2009 [PMID: 19458041]

Review for TGN38 Antibody (NB300-575) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB300-575:
Filter by Applications
ICC
(1)
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Filter by Species
Rat
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry TGN38 NB300-575
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5
reviewed by:
Verified Customer
ICC Rat 01/06/2020
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Summary

ApplicationImmunocytochemistry
Sample TestedINS-1
SpeciesRat
LotUB275575

Product General Protocols

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Recent Reviews

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Verified Customer
01/06/2020
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol TGOLN2
Entrez