TGN38 Antibody (2F7.1)

Images

 
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - HeLa and COS7 cells were rinsed with PBS and fixed with -20C methanol for 5 minutes. Cells were incubated with anti-TGN38 antibody diluted ...read more
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Staining of TGN-38 in NRK cells.
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Analysis of Phalloidin (green) and TGN38 (purple) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 ...read more
Immunocytochemistry/ Immunofluorescence: TGN38 Antibody (2F7.1) [NB300-575] - Staining in NS-1 Cells.

Product Details

Summary
Reactivity Hu, Mu, Rt, ChHa, Mk, RbSpecies Glossary
Applications WB, B/N, Flow, ICC/IF, IHC, IHC-P, ICC, IF
Clone
2F7.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

TGN38 Antibody (2F7.1) Summary

Immunogen
Synthetic peptide corresponding to residues L(1) P S A S K P N N T S S E N N P P(17) C of rat TGN38.
Marker
TGN Marker
Specificity
Detects Trans Golgi Network (TGN) 38 from human and monkey samples and both recombinant and endogenous rat samples.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TGOLN2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Blocking/Neutralizing 50 - 200 molar excess
  • Flow Cytometry 1:20 - 1:50
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Immunocytochemistry 1:1000
  • Immunofluorescence 1:50 - 1:200
Application Notes
IP usage was reported in scientific literature (PMID: 10514494). WB: Detects an approx. 38 kDa protein representing deglycosylated recombinant rat TGN38. Under normal WB conditions, Detects an 85-95 kDa band depending on cell type. IF: Staining of TGN38 in NRK cells results in staining predominantly in the TGN. IF: it has been shown that methanol works best as a fixative.
Publications
Read Publications using
NB300-575 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Chinese Hamster reactivity reported in scientific literature (PMID: 11408588). Rabbit reactivity reported in scientific literature (PMID: 10514494). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites diluted with PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for TGN38 Antibody (2F7.1)

  • TGN38
  • TGN46
  • TGN46TGN38 homolog
  • TGN48MGC14722
  • TGN51
  • TGN5148, 51kD isoforms)
  • Tgoln1
  • Tgoln2
  • trans-golgi network protein 2
  • Trans-Golgi network protein TGN51
  • Ttgn1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, ChHa, Mk, Rb
Applications: WB, B/N, Flow, ICC/IF, IHC, IHC-P, ICC, IF

Publications for TGN38 Antibody (NB300-575)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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TGN38 NB300-575

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Bioinformatics

Gene Symbol TGOLN2
Entrez