TRA-1-60(R) Antibody (TRA-1-60)


Western Blot: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - Whole cell protein from human ES cells (lane 1), human NTERA-2 cells (lane 2), mouse embryonic fibroblasts (MEF, lane 3) and mouse V6.5 ES cells (lane 4, more
Immunocytochemistry/ Immunofluorescence: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - Human embryonic stem cells stained with TRA-1-60 antibody (Cat# NB100-730) detected with Alexa Fluor 488 anti-mouse IgM more
Flow Cytometry: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - Staining of NTERA-2 cells using NB100-730at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgM secondary antibody.
Western Blot: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - Analysis of NB100-730 expression in NTERA-2 cl.D1 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - NTERA-2 cells stained NB100-730(Green) detected with DyLight Fluor 488 conjugated anti-mouse IgM secondary antibody. Nuclei more

Product Details

Reactivity Hu, Mu(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IP
1 mg/ml

TRA-1-60(R) Antibody (TRA-1-60) Summary

Human embryonal carcinoma cell line 2102Ep.
Cell Membrane
Embryonic Stem Cell Marker
Specific for the neuraminidase resistant form of the TRA-1-60 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. TRA-1-60 reacts with a sialidase-sensitive epitope.
IgM purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
1 mg/ml
IgM purified


  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:10-1:500
Application Notes
This TRA-1-60 (R) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western blot.
Read Publications using
NB100-730 in the following applications:

Reactivity Notes

Human. No immunoreactivity is seen with murine EC, EG or ES cells.

Alternate Names for TRA-1-60(R) Antibody (TRA-1-60)

  • TRA160(R)


TRA-1-60 is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors. Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan. Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRA-1-60 (R) Antibody (NB100-730)(5)

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Product General Protocols

View specific protocols for TRA-1-60 (R) Antibody (NB100-730): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRA-1-60 (R) Antibody (NB100-730). (Showing 1 - 1 of 1 FAQs).

  1. We would like to know the host of the following antibodies, is BALC/c mouse or not?
    • The lab has informed me that these were both produced in BALB/c mice.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-730AF405
Alexa Fluor (R) 488 Labeled NB100-730AF488
Alexa Fluor (R) 647 Labeled NB100-730AF647
Alexa Fluor (R) 700 Labeled NB100-730AF700
Allophycocyanin Labeled NB100-730APC
Biotin Labeled NB100-730B
DyLight 350 Labeled NB100-730UV
DyLight 405 Labeled NB100-730V
DyLight 405LS Labeled NB100-730V3
DyLight 488 Labeled NB100-730G
DyLight 550 Labeled NB100-730R
DyLight 650 Labeled NB100-730C
DyLight 680 Labeled NB100-730FR
DyLight 755 Labeled NB100-730IR
FITC Labeled NB100-730F
HRP Labeled NB100-730H
PE Labeled NB100-730PE
PerCP Labeled NB100-730PCP

Additional TRA-1-60(R) Antibody (TRA-1-60) Products

TRA-1-60(R) NB100-730

Research Areas for TRA-1-60 (R) Antibody (NB100-730)

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Blogs on TRA-1-60(R)

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