TRA-1-60® Antibody (TRA-1-60)

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Western Blot: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - Whole cell protein from human ES cells (lane 1), human NTERA-2 cells (lane 2), mouse embryonic fibroblasts (MEF, lane 3) and mouse V6.5 ES cells (lane 4, ...read more
Immunocytochemistry/ Immunofluorescence: TRA-1-60(R) Antibody (TRA-1-60) [NB100-730] - TRA-1-60(R) was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using ...read more
Flow Cytometry: TRA-1-60(R) Antibody (TRA-1-60) [NB100-730] - BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-60 Neuraminidase Resistant Epitope Monoclonal Antibody (Catalog # NB100-730, filled ...read more
Western Blot: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - Analysis of NB100-730 expression in NTERA-2 cl.D1 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730] - Human embryonic stem cells stained with TRA-1-60 antibody (Cat# NB100-730) detected with Alexa Fluor 488 anti-mouse IgM ...read more

Product Details

Summary
Reactivity Hu, Mu(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP
Clone
TRA-1-60
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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Catalog# & Formulation Size Price

TRA-1-60® Antibody (TRA-1-60) Summary

Immunogen
Human embryonal carcinoma cell line 2102Ep.
Localization
Cell Membrane
Marker
Embryonic Stem Cell Marker
Specificity
Specific for the neuraminidase resistant form of the TRA-1-60 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). TRA-1-60 reacts with a sialidase-sensitive epitope.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA reported in scientific literature (PMID 30617132)
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 33234532)
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Control
Podocalyxin Like Overexpression Lysate
Publications
Read Publications using
NB100-730 in the following applications:

Reactivity Notes

No immunoreactivity is seen with murine EC, EG or ES cells.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
IgM purified

Alternate Names for TRA-1-60(R) Antibody (TRA-1-60)

  • RA160
  • TRA160(R)
  • TRA-1-60(R)
  • tra61

Background

TRA-1-60 is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors. Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan. Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRA-1-60(R) Antibody (NB100-730)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ELISA, FLOW, ICC/IF, IHC-P, WB.


Filter By Application
ELISA
(1)
FLOW
(3)
ICC/IF
(7)
IHC-P
(2)
WB
(1)
All Applications
Filter By Species
Human
(12)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB100-730 Applications Species
Wisniewski L, Braak S, Klamer Z et al. Heterogeneity of glycan biomarker clusters as an indicator of recurrence in pancreatic cancer Frontiers in oncology 2023-04-12 [PMID: 37124496] (IHC-P, Human) IHC-P Human
McNeill RV, Radtke F, Nieberler M et al. Generation of four human induced pluripotent stem cells derived from ADHD patients carrying different genotypes for the risk SNP rs1397547 in the ADHD-associated gene ADGRL3 Stem cell research 2023-01-05 [PMID: 36640473] (ICC/IF, Mouse)

Details:
Dilution used in ICC 1:100
ICC/IF Mouse
Calabrese D, Roma G, Bergling S et al. Liver biopsy derived induced pluripotent stem cells provide unlimited supply for the generation of hepatocyte-like cells PLoS One. 2019-01-01 [PMID: 31465481] (ICC/IF, Human, Mouse) ICC/IF Human, Mouse
Staal B, Liu Y, Barnett D et al. The sTRA Plasma Biomarker: Blinded Validation of Improved Accuracy over CA19-9 in Pancreatic Cancer Diagnosis Clin. Cancer Res. 2019-01-07 [PMID: 30617132] (ELISA, Human) ELISA Human
Palladino VS, Subrata NOC, Geburtig-Chiocchetti A et al. Generation of human induced pluripotent stem cell lines (hiPSC) from one bipolar disorder patient carrier of a DGKH risk haplotype and one non-risk-variant-carrier bipolar disorder patient. Stem Cell Res 2018-09-17 [PMID: 30266033] (ICC/IF, Human) ICC/IF Human
Rossbach B, Hildebrand L, El-Ahmad L. Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector. Stem Cell Research. 2016-03-01 [PMID: 28677531] (ICC/IF, Human)

Details:
This citation used the DyLight 650 form of this antibody.
ICC/IF Human
Rossbach B, Hildebrand L, El-Ahmad L. Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector Stem Cell Research 2016-01-01 [PMID: 27638042] (ICC/IF, Human)

Details:
This reference used the DyLight 650 version of NB100-1833.
ICC/IF Human
Rossbach B, Hildebrand L, El-Ahmad L. Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector. Stem Cell Res 2016-06-28 [PMID: 27345993] (ICC/IF, Human) ICC/IF Human
Tang H, Partyka K, Hsueh P et al. Glycans Related to the CA19-9 Antigen Are Increased in Distinct Subsets of Pancreatic Cancers and Improve Diagnostic Accuracy Over CA19-9. CMGH. 2015-12-20 [PMID: 26998508]
Badcock, G et al. The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. Cancer Res;59(18):4715-9. 1999-09-15 [PMID: 10493530] (WB, Human) WB Human
Show All 16 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRA-1-60(R) Antibody (NB100-730). (Showing 1 - 1 of 1 FAQs).

  1. We would like to know the host of the following antibodies, is BALC/c mouse or not?
    • The lab has informed me that these were both produced in BALB/c mice.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional TRA-1-60(R) Products

Array NB100-730

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