Palladin Antibody (1E6)


Western Blot: Palladin Antibody (1E6) [NBP1-25959] - Western blot analysis of HeLa (A), A431 (B), NIH3T3 (C), and PC-12 (D) cell line extracts using Palladin antibody at 1ug/ml.
Immunocytochemistry/ Immunofluorescence: Palladin Antibody (1E6) [NBP1-25959] - Immunofluorescence on a7r5 cells.
Western Blot: Palladin Antibody (1E6) [NBP1-25959] - MDA-MB-231 lysate.

Product Details

Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
1 mg/ml

Palladin Antibody (1E6) Summary

Endogenous Palladin immunoprecipitated from chicken embryo fibroblasts.
Cytoplasm, cytoskeleton. Note: Localizes to stress fibers and Z-disks. Cell junction, focal adhesion. Cell projection, ruffle. Cell projection, lamellipodium.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:20-1:100
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 0.05 ml / 2 ml lysate
Application Notes
This Palladin (1E6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB a band is seen at ~85 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NBP1-25959 in the following applications:

  • 4 publications
  • 4 publications
  • IP
    2 publications
  • WB
    9 publications

Reactivity Notes

Chicken, Human, Mouse, Rat.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
1 mg/ml
Protein G purified

Alternate Names for Palladin Antibody (1E6)

  • CGI151
  • CGI-151
  • FLJ22190
  • FLJ38193
  • KIAA0992FLJ39139
  • MYN
  • myoneurin
  • Palladin
  • palladin, cytoskeletal associated protein
  • PNCA1
  • Sarcoma antigen NY-SAR-77
  • SIH002
  • SIH002FLJ61376


Palladin (PALLD) is an actin associated cytoskeletal protein which is widely expressed and found in stress fibers, focal adhesions, growth cones, Z-discs, and other actin-based subcellular structures. It belongs to a small gene family that includes the Z-disc proteins myopalladin and myotilin, all of which share similar Ig-like domains and interact with specific molecular partners: all three bind to alpha-actinin; in addition, palladin also binds to VASP and ezrin, myotilin binds to filamin and actin, and myopalladin also binds to nebulin and CARP. While myotilin and myopalladin are most abundant in skeletal and cardiac muscle, palladin is ubiquitously expressed in the organs of developing vertebrates. Palladin functions as a molecular scaffold as well as an actin cross linker, and its nuclear presence and ability to translocate to the nucleus upon stimulation reflects that palladin has signaling functions in excitation-transcription. It interacts with EPS8, LASP1, VASP, ACTN, ARGBP2, LPP, PFN1, SPIN90, SRC and EZR, and plays key role in smooth muscle differentiation, migration, contraction, embryonic development and in some pathological conditions including cardiovascular diseases as well as cancers. Palladin levels are typically upregulated actively migrating cells e.g. developing vertebrate embryos, in cells along a wound edge, and in metastatic cancer cells; and genetic variations in PALLD gene have been linked with susceptibility to pancreatic cancer type 1 (PNCA1) and myocardial infarction.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Palladin Antibody (NBP1-25959)(14)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Chicken.

We have publications tested in 4 applications: ICC/IF, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NBP1-25959 Applications Species
Bhome R, Goh RW, Bullock MD et al. Exosomal microRNAs derived from colorectal cancer-associated fibroblasts: role in driving cancer progression Aging (Albany NY) 2017 Dec 28 [PMID: 29283887] (WB, Human) WB Human
Li S, Liu C, Gu L et al. Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36. Open Biol. Aug 1 2016 [PMID: 27512143] (Mouse) Mouse
Morishige N, Murata S, Nakamura Y et al. Coordinated Regulation of Palladin and alpha-Smooth Muscle Actin by Transforming Growth Factor-b in Human Corneal Fibroblasts. Invest. Ophthalmol. Vis. Sci. Jul 8 1905 [PMID: 27367503] (ICC/IF, Human) ICC/IF Human
Nguyen NU, Wang HV. Dual roles of palladin protein in in vitro myogenesis: inhibition of early induction but promotion of myotube maturation PLoS ONE 2015 Apr 16 [PMID: 25875253] (WB, Mouse) WB Mouse
Chang EH, Gasim AH, Kerber ML et al. Palladin is Upregulated in Kidney Disease and Contributes to Epithelial Cell Migration After Injury Sci Rep. 2015 Jan 09 [PMID: 25573828] (WB, Mouse, Human) WB Mouse, Human
van Steenbeek FG, Van den Bossche L, Grinwis GC et al. Aberrant Gene Expression in Dogs with Portosystemic Shunts. PLoS One 2013 [PMID: 23451256] (IHC-P, Canine)

Using the DyLight 488 conjugated version of NBP1-25959, catalog number NBP1-25959G.
IHC-P Canine
Brentnall TA, Lai LA, Coleman J et al. Arousal of cancer-associated stroma: overexpression of palladin activates fibroblasts to promote tumor invasion. PLoS ONE 20127(1):e30219. [PMID: 22291919] (IHC-P, ICC/IF, Human) IHC-P, ICC/IF Human
Goicoechea SM, Bednarski B, Stack C, Cowan DW, Volmar K, Thorne L, Cukierman E, Rustgi AK, Brentnall T, Hwang RF, McCulloch CA, Yeh JJ, Bentrem DJ, Hochwald SN, Hingorani SR, Kim HJ, Otey CA. Isoform-specific upregulation of palladin in human and murine pancreas tumors. PLoS One;5(4):e10347. 2010 Apr 26. [PMID: 20436683] (WB, IHC-P, Human) WB, IHC-P Human
Goicoechea, S et al. Palladin contributes to invasive motility in human breast cancer cells. Oncogene;28(4):587-98. 2009 Jan 29. [PMID: 18978809] (WB, Human) WB Human
Endlich N, Otey CA, Kriz W, Endlich K. Movement of stress fibers away from focal adhesions identifies focal adhesions as sites of stress fiber assembly in stationary cells. Cell Motil Cytoskeleton;64(12):966-76. 2007 Dec. [PMID: 17868136] (ICC/IF, Mouse) ICC/IF Mouse
Show All 14 Publications.

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Product General Protocols

View specific protocols for Palladin Antibody (NBP1-25959): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Palladin Antibody (NBP1-25959). (Showing 1 - 1 of 1 FAQs).

  1. What is the MW of the Palladin when run on a native gel?
    • The only QC experiments we have for this antibody in WB were run under reducing conditions, so we do not know the MW size when run on a native gel. I checked our WB publication list and did not see any evidence of the size of Palladin on a native gel. 

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-25959AF405
Alexa Fluor 488 Labeled NBP1-25959AF488
Alexa Fluor 647 Labeled NBP1-25959AF647
Alexa Fluor 700 Labeled NBP1-25959AF700
Biotin Labeled NBP1-25959B
DyLight 350 Labeled NBP1-25959UV
DyLight 405 Labeled NBP1-25959V
DyLight 488 Labeled NBP1-25959G
DyLight 550 Labeled NBP1-25959R
DyLight 650 Labeled NBP1-25959C
DyLight 680 Labeled NBP1-25959FR
DyLight 755 Labeled NBP1-25959IR
FITC Labeled NBP1-25959F
HRP Labeled NBP1-25959H

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Gene Symbol PALLD