Palladin Antibody (1E6)


Genetic Strategies: Knockdown Validated: Palladin Antibody (1E6) [NBP1-25959] - C2C12 cells were transfected with shRNA targeting palladin or nontargeting shRNA as a control. Stable transfectants were submitted more
Western Blot: Palladin Antibody (1E6) [NBP1-25959] - MDA-MB-231 lysate.
Genetic Strategies: Knockdown Validated: Palladin Antibody (1E6) [NBP1-25959] - Western blots analysis of C2C12 cells labeled with a monoclonal antibody against 90-kDa palladin or a polyclonal antibody against more
Immunocytochemistry/ Immunofluorescence: Palladin Antibody (1E6) [NBP1-25959] - Immunofluorescence on a7r5 cells.
Western Blot: Palladin Antibody (1E6) [NBP1-25959] - Western blot analysis of HeLa (A), A431 (B), NIH3T3 (C), and PC-12 (D) cell line extracts using Palladin antibody at 1ug/ml.
Immunohistochemistry-Paraffin: Palladin Antibody (1E6) [NBP1-25959] - Palladin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Palladin (1E6) Monoclonal Antibody (Catalog more
Flow Cytometry: Palladin Antibody (1E6) [NBP1-25959] - An intracellular stain was performed on HeLa cells with Palladin Antibody [1E6] NBP1-25959AF488 (blue) and a matched isotype control (orange). Cells were fixed with more

Product Details

Reactivity Hu, Mu, Rt, Ca, ChSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, KD
1 mg/ml
Validated by:

Genetic Strategies


Order Details

Palladin Antibody (1E6) Summary

Endogenous Palladin immunoprecipitated from chicken embryo fibroblasts.
Cytoplasm, cytoskeleton. Note: Localizes to stress fibers and Z-disks. Cell junction, focal adhesion. Cell projection, ruffle. Cell projection, lamellipodium.
Protein G purified
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  • Flow Cytometry 5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:20-1:100
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 0.05 ml / 2 ml lysate
  • Knockdown Validated
  • Western Blot 1 ug/ml
Application Notes
In WB a band is seen at ~85 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NBP1-25959 in the following applications:

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 23451256).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
1 mg/ml
Protein G purified

Alternate Names for Palladin Antibody (1E6)

  • CGI151
  • CGI-151
  • FLJ22190
  • FLJ38193
  • KIAA0992FLJ39139
  • MYN
  • myoneurin
  • Palladin
  • palladin, cytoskeletal associated protein
  • PNCA1
  • Sarcoma antigen NY-SAR-77
  • SIH002
  • SIH002FLJ61376


Palladin (PALLD) is an actin associated cytoskeletal protein which is widely expressed and found in stress fibers, focal adhesions, growth cones, Z-discs, and other actin-based subcellular structures. It belongs to a small gene family that includes the Z-disc proteins myopalladin and myotilin, all of which share similar Ig-like domains and interact with specific molecular partners: all three bind to alpha-actinin; in addition, palladin also binds to VASP and ezrin, myotilin binds to filamin and actin, and myopalladin also binds to nebulin and CARP. While myotilin and myopalladin are most abundant in skeletal and cardiac muscle, palladin is ubiquitously expressed in the organs of developing vertebrates. Palladin functions as a molecular scaffold as well as an actin cross linker, and its nuclear presence and ability to translocate to the nucleus upon stimulation reflects that palladin has signaling functions in excitation-transcription. It interacts with EPS8, LASP1, VASP, ACTN, ARGBP2, LPP, PFN1, SPIN90, SRC and EZR, and plays key role in smooth muscle differentiation, migration, contraction, embryonic development and in some pathological conditions including cardiovascular diseases as well as cancers. Palladin levels are typically upregulated actively migrating cells e.g. developing vertebrate embryos, in cells along a wound edge, and in metastatic cancer cells; and genetic variations in PALLD gene have been linked with susceptibility to pancreatic cancer type 1 (PNCA1) and myocardial infarction.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Palladin Antibody (NBP1-25959)(19)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Canine, Chicken, Mammal.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NBP1-25959 Applications Species
Mayer O, Bugis J, Kozlova D et al. Cytoskeletal Protein Palladin in Adult Gliomas Predicts Disease Incidence, Progression, and Prognosis Cancers 2022-10-19 [PMID: 36291914] (IF/IHC, Mouse) IF/IHC Mouse
Davidson B, Bock Aj, Holth A, Nymoen Da Expression of palladin is associated with disease progression in metastatic high-grade serous carcinoma Cytopathology 2020-08-02 [PMID: 32741023] (IF/IHC, Human) IF/IHC Human
Bhome R, Goh RW, Bullock MD et al. Exosomal microRNAs derived from colorectal cancer-associated fibroblasts: role in driving cancer progression Aging (Albany NY) 2017-12-28 [PMID: 29283887] (WB, Human) WB Human
Li S, Liu C, Gu L et al. Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36. Open Biol. 2016-08-01 [PMID: 27512143] (Mouse) Mouse
Morishige N, Murata S, Nakamura Y et al. Coordinated Regulation of Palladin and alpha-Smooth Muscle Actin by Transforming Growth Factor-b in Human Corneal Fibroblasts. Invest. Ophthalmol. Vis. Sci. 1905-07-08 [PMID: 27367503] (ICC/IF, Human) ICC/IF Human
Chang EH, Gasim AH, Kerber ML et al. Palladin is Upregulated in Kidney Disease and Contributes to Epithelial Cell Migration After Injury Sci Rep. 2015-01-09 [PMID: 25573828] (WB, Human, Mouse) WB Human, Mouse
van Steenbeek FG, Van den Bossche L, Grinwis GC et al. Aberrant Gene Expression in Dogs with Portosystemic Shunts. PLoS One 2013-01-01 [PMID: 23451256] (IHC-P, Canine) IHC-P Canine
Dhanda A Morphological and functional characterization of host proteins during infections by actin-hijacking bacterial pathogens Thesis 2020-01-01 (ICC/IF, Human, Mammal)

Potorous tridactylus kidney
ICC/IF Human, Mammal
Dhanda AS, Vogl AW, Albraiki SE et al. Palladin Compensates for the Arp2/3 Complex and Supports Actin Structures during Listeria Infections MBio 2018-04-10 [PMID: 29636431] (ICC/IF, WB, Human) ICC/IF, WB Human
Nguyen NU, Wang HV. Dual roles of palladin protein in in vitro myogenesis: inhibition of early induction but promotion of myotube maturation PLoS ONE 2015-04-16 [PMID: 25875253] (WB, Mouse) WB Mouse
Show All 19 Publications.

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Product General Protocols

View specific protocols for Palladin Antibody (NBP1-25959): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Palladin Antibody (NBP1-25959). (Showing 1 - 1 of 1 FAQs).

  1. What is the MW of the Palladin when run on a native gel?
    • The only QC experiments we have for this antibody in WB were run under reducing conditions, so we do not know the MW size when run on a native gel. I checked our WB publication list and did not see any evidence of the size of Palladin on a native gel. 

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Gene Symbol PALLD