Palladin Antibody (1E6)

Images

 
Genetic Strategies: Knockdown Validated: Palladin Antibody (1E6) [NBP1-25959] - C2C12 cells were transfected with shRNA targeting palladin or nontargeting shRNA as a control. Stable transfectants were submitted ...read more
Western Blot: Palladin Antibody (1E6) [NBP1-25959] - MDA-MB-231 lysate.
Genetic Strategies: Knockdown Validated: Palladin Antibody (1E6) [NBP1-25959] - Western blots analysis of C2C12 cells labeled with a monoclonal antibody against 90-kDa palladin or a polyclonal antibody against ...read more
Immunocytochemistry/ Immunofluorescence: Palladin Antibody (1E6) [NBP1-25959] - Immunofluorescence on a7r5 cells.
Western Blot: Palladin Antibody (1E6) [NBP1-25959] - Western blot analysis of HeLa (A), A431 (B), NIH3T3 (C), and PC-12 (D) cell line extracts using Palladin antibody at 1ug/ml.
Immunohistochemistry-Paraffin: Palladin Antibody (1E6) [NBP1-25959] - Palladin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Palladin (1E6) Monoclonal Antibody (Catalog ...read more
Flow Cytometry: Palladin Antibody (1E6) [NBP1-25959] - An intracellular stain was performed on HeLa cells with Palladin Antibody [1E6] NBP1-25959AF488 (blue) and a matched isotype control (orange). Cells were fixed with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, ChSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP, KD
Clone
1E6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

Palladin Antibody (1E6) Summary

Immunogen
Endogenous Palladin immunoprecipitated from chicken embryo fibroblasts.
Localization
Cytoplasm, cytoskeleton. Note: Localizes to stress fibers and Z-disks. Cell junction, focal adhesion. Cell projection, ruffle. Cell projection, lamellipodium.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PALLD
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:20-1:100
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 0.05 ml / 2 ml lysate
  • Knockdown Validated
  • Western Blot 1 ug/ml
Application Notes
This Palladin (1E6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB a band is seen at ~85 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-25959 in the following applications:

  • 6 publications
  • IHC
    1 publication
  • 4 publications
  • IP
    2 publications
  • WB
    11 publications

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 23451256).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Palladin Antibody (1E6)

  • CGI151
  • CGI-151
  • FLJ22190
  • FLJ38193
  • KIAA0992FLJ39139
  • MYN
  • myoneurin
  • Palladin
  • palladin, cytoskeletal associated protein
  • PALLD
  • PNCA1
  • Sarcoma antigen NY-SAR-77
  • SIH002
  • SIH002FLJ61376

Background

Palladin (PALLD) is an actin associated cytoskeletal protein which is widely expressed and found in stress fibers, focal adhesions, growth cones, Z-discs, and other actin-based subcellular structures. It belongs to a small gene family that includes the Z-disc proteins myopalladin and myotilin, all of which share similar Ig-like domains and interact with specific molecular partners: all three bind to alpha-actinin; in addition, palladin also binds to VASP and ezrin, myotilin binds to filamin and actin, and myopalladin also binds to nebulin and CARP. While myotilin and myopalladin are most abundant in skeletal and cardiac muscle, palladin is ubiquitously expressed in the organs of developing vertebrates. Palladin functions as a molecular scaffold as well as an actin cross linker, and its nuclear presence and ability to translocate to the nucleus upon stimulation reflects that palladin has signaling functions in excitation-transcription. It interacts with EPS8, LASP1, VASP, ACTN, ARGBP2, LPP, PFN1, SPIN90, SRC and EZR, and plays key role in smooth muscle differentiation, migration, contraction, embryonic development and in some pathological conditions including cardiovascular diseases as well as cancers. Palladin levels are typically upregulated actively migrating cells e.g. developing vertebrate embryos, in cells along a wound edge, and in metastatic cancer cells; and genetic variations in PALLD gene have been linked with susceptibility to pancreatic cancer type 1 (PNCA1) and myocardial infarction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-38361
Species: Hu
Applications: IHC, IHC-P
NBP2-00555
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-38924
Species: Hu
Applications: IHC, IHC-P
NB200-106
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP2-52977
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NB600-302
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
MAB2476
Species: Hu
Applications: IHC, WB
NBP3-09537
Species: Hu
Applications: WB
NBP2-45626
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-09512
Species: Hu
Applications: WB
NBP1-84443
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-14292
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-89932
Species: Hu
Applications: IHC, IHC-P
NB100-793
Species: Hu, Rt
Applications: IF, IHC, IHC-P, PEP-ELISA, WB
NBP1-89549
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
DNST0
Species: Hu
Applications: ELISA
NBP1-85299
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-25959
Species: Hu, Mu, Rt, Ca, Ch
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, KD

Publications for Palladin Antibody (NBP1-25959)(18)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Canine, Chicken, Mammal.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(6)
IHC
(1)
IHC-P
(4)
IP
(2)
WB
(11)
All Applications
Filter By Species
Human
(12)
Mouse
(6)
Rat
(1)
Canine
(1)
Chicken
(1)
Mammal
(1)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NBP1-25959 Applications Species
Davidson B, Bock Aj, Holth A, Nymoen Da Expression of palladin is associated with disease progression in metastatic high-grade serous carcinoma Cytopathology Aug 2 2020 [PMID: 32741023] (IHC, Human) IHC Human
Bhome R, Goh RW, Bullock MD et al. Exosomal microRNAs derived from colorectal cancer-associated fibroblasts: role in driving cancer progression Aging (Albany NY) 2017 Dec 28 [PMID: 29283887] (WB, Human) WB Human
Li S, Liu C, Gu L et al. Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36. Open Biol. Aug 1 2016 [PMID: 27512143] (Mouse) Mouse
Morishige N, Murata S, Nakamura Y et al. Coordinated Regulation of Palladin and alpha-Smooth Muscle Actin by Transforming Growth Factor-b in Human Corneal Fibroblasts. Invest. Ophthalmol. Vis. Sci. Jul 8 1905 [PMID: 27367503] (ICC/IF, Human) ICC/IF Human
Chang EH, Gasim AH, Kerber ML et al. Palladin is Upregulated in Kidney Disease and Contributes to Epithelial Cell Migration After Injury Sci Rep. 2015 Jan 09 [PMID: 25573828] (WB, Mouse, Human) WB Mouse, Human
van Steenbeek FG, Van den Bossche L, Grinwis GC et al. Aberrant Gene Expression in Dogs with Portosystemic Shunts. PLoS One 2013 [PMID: 23451256] (IHC-P, Canine)

Details:
Using the DyLight 488 conjugated version of NBP1-25959, catalog number NBP1-25959G.
IHC-P Canine
Dhanda A Morphological and functional characterization of host proteins during infections by actin-hijacking bacterial pathogens Thesis Jan 1 2020 (ICC/IF, Human, Mammal)

Details:
Potorous tridactylus kidney
ICC/IF Human, Mammal
Dhanda AS, Vogl AW, Albraiki SE et al. Palladin Compensates for the Arp2/3 Complex and Supports Actin Structures during Listeria Infections MBio 2018 Apr 10 [PMID: 29636431] (WB, ICC/IF, Human) WB, ICC/IF Human
Stasiak M, Gawryś K, Popielarski M et al. Differential Quantitative Proteomics of Human Microvascular Endothelial Cells 1 by iTRAQ Reveals Palladin to be a New Biomarker During TGF-beta 1 Induced Endothelial Mesenchymal Transition Journal of Proteomics & Bioinformatics 2017 Oct 11 (WB, Human) WB Human
Nguyen NU, Wang HV. Dual roles of palladin protein in in vitro myogenesis: inhibition of early induction but promotion of myotube maturation PLoS ONE 2015 Apr 16 [PMID: 25875253] (WB, Mouse) WB Mouse
Show All 18 Publications.

Reviews for Palladin Antibody (NBP1-25959) (0)

There are no reviews for Palladin Antibody (NBP1-25959). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for Palladin Antibody (NBP1-25959): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Palladin Antibody (NBP1-25959). (Showing 1 - 1 of 1 FAQs).

  1. What is the MW of the Palladin when run on a native gel?
    • The only QC experiments we have for this antibody in WB were run under reducing conditions, so we do not know the MW size when run on a native gel. I checked our WB publication list and did not see any evidence of the size of Palladin on a native gel. 

Secondary Antibodies

 

Isotype Controls

Additional Palladin Products

Bioinformatics Tool for Palladin Antibody (NBP1-25959)

Discover related pathways, diseases and genes to Palladin Antibody (NBP1-25959). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Palladin Antibody (NBP1-25959)

Discover more about diseases related to Palladin Antibody (NBP1-25959).
 

Pathways for Palladin Antibody (NBP1-25959)

View related products by pathway.

Research Areas for Palladin Antibody (NBP1-25959)

Find related products by research area.

Blogs on Palladin

There are no specific blogs for Palladin, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Palladin Antibody (1E6) and receive a gift card or discount.

Bioinformatics

Gene Symbol PALLD
Entrez
Uniprot