Palladin Antibody (1E6)

Images

 
Western Blot: Palladin Antibody (1E6) [NBP1-25959] - Western blot analysis of HeLa (A), A431 (B), NIH3T3 (C), and PC-12 (D) cell line extracts using Palladin antibody at 1ug/ml.
Immunocytochemistry/ Immunofluorescence: Palladin Antibody (1E6) [NBP1-25959] - Immunofluorescence on a7r5 cells.
Western Blot: Palladin Antibody (1E6) [NBP1-25959] - MDA-MB-231 lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
1E6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Palladin Antibody (1E6) Summary

Immunogen
Endogenous Palladin immunoprecipitated from chicken embryo fibroblasts.
Localization
Cytoplasm, cytoskeleton. Note: Localizes to stress fibers and Z-disks. Cell junction, focal adhesion. Cell projection, ruffle. Cell projection, lamellipodium.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PALLD
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:20-1:100
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation 0.05 ml / 2 ml lysate
Application Notes
This Palladin (1E6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB a band is seen at ~85 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-25959 in the following applications:

  • 4 publications
  • 4 publications
  • IP
    2 publications
  • WB
    8 publications

Reactivity Notes

Chicken, Human, Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Palladin Antibody (1E6)

  • CGI151
  • CGI-151
  • FLJ22190
  • FLJ38193
  • KIAA0992FLJ39139
  • MYN
  • myoneurin
  • Palladin
  • palladin, cytoskeletal associated protein
  • PALLD
  • PNCA1
  • Sarcoma antigen NY-SAR-77
  • SIH002
  • SIH002FLJ61376

Background

Palladin (PALLD) is an actin associated cytoskeletal protein which is widely expressed and found in stress fibers, focal adhesions, growth cones, Z-discs, and other actin-based subcellular structures. It belongs to a small gene family that includes the Z-disc proteins myopalladin and myotilin, all of which share similar Ig-like domains and interact with specific molecular partners: all three bind to alpha-actinin; in addition, palladin also binds to VASP and ezrin, myotilin binds to filamin and actin, and myopalladin also binds to nebulin and CARP. While myotilin and myopalladin are most abundant in skeletal and cardiac muscle, palladin is ubiquitously expressed in the organs of developing vertebrates. Palladin functions as a molecular scaffold as well as an actin cross linker, and its nuclear presence and ability to translocate to the nucleus upon stimulation reflects that palladin has signaling functions in excitation-transcription. It interacts with EPS8, LASP1, VASP, ACTN, ARGBP2, LPP, PFN1, SPIN90, SRC and EZR, and plays key role in smooth muscle differentiation, migration, contraction, embryonic development and in some pathological conditions including cardiovascular diseases as well as cancers. Palladin levels are typically upregulated actively migrating cells e.g. developing vertebrate embryos, in cells along a wound edge, and in metastatic cancer cells; and genetic variations in PALLD gene have been linked with susceptibility to pancreatic cancer type 1 (PNCA1) and myocardial infarction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Palladin Antibody (NBP1-25959)(13)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Chicken.

We have publications tested in 4 applications: ICC/IF, IHC-P, IP, WB.


Filter By Application
ICC/IF
(4)
IHC-P
(4)
IP
(2)
WB
(8)
All Applications
Filter By Species
Human
(7)
Mouse
(6)
Rat
(1)
Canine
(1)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NBP1-25959 Applications Species
Li S, Liu C, Gu L et al. Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36. Open Biol. Aug 1 2016 [PMID: 27512143] (Mouse) Mouse
Morishige N, Murata S, Nakamura Y et al. Coordinated Regulation of Palladin and alpha-Smooth Muscle Actin by Transforming Growth Factor-b in Human Corneal Fibroblasts. Invest. Ophthalmol. Vis. Sci. Jul 8 1905 [PMID: 27367503] (ICC/IF, Human) ICC/IF Human
Nguyen NU, Wang HV. Dual roles of palladin protein in in vitro myogenesis: inhibition of early induction but promotion of myotube maturation PLoS ONE 2015 Apr 16 [PMID: 25875253] (WB, Mouse) WB Mouse
Chang EH, Gasim AH, Kerber ML et al. Palladin is Upregulated in Kidney Disease and Contributes to Epithelial Cell Migration After Injury Sci Rep. 2015 Jan 09 [PMID: 25573828] (WB, Mouse, Human) WB Mouse, Human
van Steenbeek FG, Van den Bossche L, Grinwis GC et al. Aberrant Gene Expression in Dogs with Portosystemic Shunts. PLoS One 2013 [PMID: 23451256] (IHC-P, Canine)

Details:
Using the DyLight 488 conjugated version of NBP1-25959, catalog number NBP1-25959G.
IHC-P Canine
Brentnall TA, Lai LA, Coleman J et al. Arousal of cancer-associated stroma: overexpression of palladin activates fibroblasts to promote tumor invasion. PLoS ONE 20127(1):e30219. [PMID: 22291919] (IHC-P, ICC/IF, Human) IHC-P, ICC/IF Human
Goicoechea SM, Bednarski B, Stack C, Cowan DW, Volmar K, Thorne L, Cukierman E, Rustgi AK, Brentnall T, Hwang RF, McCulloch CA, Yeh JJ, Bentrem DJ, Hochwald SN, Hingorani SR, Kim HJ, Otey CA. Isoform-specific upregulation of palladin in human and murine pancreas tumors. PLoS One;5(4):e10347. 2010 Apr 26. [PMID: 20436683] (WB, IHC-P, Human) WB, IHC-P Human
Goicoechea, S et al. Palladin contributes to invasive motility in human breast cancer cells. Oncogene;28(4):587-98. 2009 Jan 29. [PMID: 18978809] (WB, Human) WB Human
Endlich N, Otey CA, Kriz W, Endlich K. Movement of stress fibers away from focal adhesions identifies focal adhesions as sites of stress fiber assembly in stationary cells. Cell Motil Cytoskeleton;64(12):966-76. 2007 Dec. [PMID: 17868136] (ICC/IF, Mouse) ICC/IF Mouse
Wall ME, Rachlin A, Otey CA, Loboa EG. Human adipose-derived adult stem cells upregulate palladin during osteogenesis and in response to cyclic tensile strain. Am J Physiol Cell Physiol;293(5):C1532-8. 2007 Nov. [PMID: 17687002] (WB, IHC-P, Human) WB, IHC-P Human
Show All 13 Publications.

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Product General Protocols

View specific protocols for Palladin Antibody (NBP1-25959): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Palladin Antibody (NBP1-25959) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-25959AF405
Alexa Fluor 488 Labeled NBP1-25959AF488
Alexa Fluor 647 Labeled NBP1-25959AF647
Alexa Fluor 700 Labeled NBP1-25959AF700
Biotin Labeled NBP1-25959B
DyLight 350 Labeled NBP1-25959UV
DyLight 405 Labeled NBP1-25959V
DyLight 488 Labeled NBP1-25959G
DyLight 550 Labeled NBP1-25959R
DyLight 650 Labeled NBP1-25959C
DyLight 680 Labeled NBP1-25959FR
DyLight 755 Labeled NBP1-25959IR
FITC Labeled NBP1-25959F
HRP Labeled NBP1-25959H

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Bioinformatics

Gene Symbol PALLD
Entrez
Uniprot