Genetic Strategies: Knockdown Validated: Palladin Antibody (1E6) [NBP1-25959] - C2C12 cells were transfected with shRNA targeting palladin or nontargeting shRNA as a control. Stable transfectants were submitted ...read more
Western Blot: Palladin Antibody (1E6) [NBP1-25959] - MDA-MB-231 lysate.
Genetic Strategies: Knockdown Validated: Palladin Antibody (1E6) [NBP1-25959] - Western blots analysis of C2C12 cells labeled with a monoclonal antibody against 90-kDa palladin or a polyclonal antibody against ...read more
Western Blot: Palladin Antibody (1E6) [NBP1-25959] - Western blot analysis of HeLa (A), A431 (B), NIH3T3 (C), and PC-12 (D) cell line extracts using Palladin antibody at 1ug/ml.
Immunohistochemistry-Paraffin: Palladin Antibody (1E6) [NBP1-25959] - Palladin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Palladin (1E6) Monoclonal Antibody (Catalog ...read more
Flow Cytometry: Palladin Antibody (1E6) [NBP1-25959] - An intracellular stain was performed on HeLa cells with Palladin Antibody [1E6] NBP1-25959AF488 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
This Palladin (1E6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB a band is seen at ~85 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
85 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP1-25959 in the following applications:
Canine reactivity reported in scientific literature (PMID: 23451256).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
Protein G purified
Alternate Names for Palladin Antibody (1E6)
palladin, cytoskeletal associated protein
Sarcoma antigen NY-SAR-77
Palladin (PALLD) is an actin associated cytoskeletal protein which is widely expressed and found in stress fibers, focal adhesions, growth cones, Z-discs, and other actin-based subcellular structures. It belongs to a small gene family that includes the Z-disc proteins myopalladin and myotilin, all of which share similar Ig-like domains and interact with specific molecular partners: all three bind to alpha-actinin; in addition, palladin also binds to VASP and ezrin, myotilin binds to filamin and actin, and myopalladin also binds to nebulin and CARP. While myotilin and myopalladin are most abundant in skeletal and cardiac muscle, palladin is ubiquitously expressed in the organs of developing vertebrates. Palladin functions as a molecular scaffold as well as an actin cross linker, and its nuclear presence and ability to translocate to the nucleus upon stimulation reflects that palladin has signaling functions in excitation-transcription. It interacts with EPS8, LASP1, VASP, ACTN, ARGBP2, LPP, PFN1, SPIN90, SRC and EZR, and plays key role in smooth muscle differentiation, migration, contraction, embryonic development and in some pathological conditions including cardiovascular diseases as well as cancers. Palladin levels are typically upregulated actively migrating cells e.g. developing vertebrate embryos, in cells along a wound edge, and in metastatic cancer cells; and genetic variations in PALLD gene have been linked with susceptibility to pancreatic cancer type 1 (PNCA1) and myocardial infarction.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Stasiak M, Gawryś K, Popielarski M et al. Differential Quantitative Proteomics of Human Microvascular Endothelial Cells 1 by iTRAQ Reveals Palladin to be a New Biomarker During TGF-beta 1 Induced Endothelial Mesenchymal Transition Journal of Proteomics & Bioinformatics 2017 Oct 11 (WB, Human)
FAQs for Palladin Antibody (NBP1-25959). (Showing 1 - 1 of 1 FAQs).
What is the MW of the Palladin when run on a native gel?
The only QC experiments we have for this antibody in WB were run under reducing conditions, so we do not know the MW size when run on a native gel. I checked our WB publication list and did not see any evidence of the size of Palladin on a native gel.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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