LOX propeptide Antibody

Images

 
Western Blot: LOX propeptide Antibody [NB110-41568] - Detection of LOPP in MC3T3-E1 cell lysate.
Immunohistochemistry-Paraffin: LOX propeptide Antibody [NB110-41568] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse intestine using 1:200 dilution of rabbit anti- LOX propeptide (LOX-PP) ...read more
Western Blot: LOX propeptide Antibody [NB110-41568] - Total protein from human and mouse brain was separated on a 12% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Immunocytochemistry/ Immunofluorescence: LOX propeptide Antibody [NB110-41568] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells ...read more
Western Blot: LOX propeptide Antibody [NB110-41568] - LOX propeptide levels analyzed in supernatants from human vascular smooth muscle cells. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

LOX propeptide Antibody Summary

Immunogen
A synthetic peptide made to an internal region of mouse LOX propeptide (residues 78-115). [UniProt# P28301]
Specificity
This antibody specifically recognizes both the pro-enzyme and pro-peptide forms of LOX protein in mouse and rat.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LOX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:200 - 1:1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1:1000
Application Notes
This LOX propeptide antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin and Western Blot where a band is seen at ~18 kDa. The strong band at 50 kDa and smear of bands that run between 46 kDa and 50 kDa are glycosylated and non-glycosylated forms of pro-lysyl oxidase. Bands between 28-36 kDa are glycosylated propeptide. The variation in MW depends on the extent of glycosylation.
Reviewed Applications
Read 2 Reviews rated 3.5
using
NB110-41568 in the following applications:

Publications
Read Publications using
NB110-41568 in the following applications:

Reactivity Notes

Human, mouse, rat, primate, and pig.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LOX propeptide Antibody

  • lysyl oxidaseEC 1.4.3.13
  • MGC105112
  • protein-lysine 6-oxidase

Background

Lysyl oxidase (LOX) is a critical enzyme in extracellular matrix biosynthesis as well as in the maintainance of normal bone phenotype. It is synthesized/secreted as a 50-kD proenzyme 'pro-LOX' which is then processed to 30-kD mature enzyme 'LOX' and 18-kD lysyl oxidase propeptide 'LOX-PP' by extracellular procollagen C-proteinases (BMP1, Tll1, and Tll2). LOX-PP has been proposed to implicate in the maintenance of inactive state of LOX within the secretory pathway and to act as intramolecular chaperones which facilitate the accurate protein folding/targeting to correct destinations. LOX-PP involves tumor suppression by inhibiting RAS-dependent cellular transformation and its expression has also been associated with inhibition of anchorage-independent progression as well as migration and growth suppression xenografts in Her-2/neu-driven breast cancer. LOX-PP also inhibits PI3K/AKT and ERK1/2 MAP kinase pathways, as well as the levels of downstream NF-kB/cyclin D1 signals in certain cancers - breast, pancreatic, lung, prostate and oral cancers. LOX-PP has also been shown to inhibit DNA synthesis in rat's VSMCs cultures, osteoblast proliferation and FGF-2 signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LOX propeptide Antibody (NB110-41568)(5)

Reviews for LOX propeptide Antibody (NB110-41568) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB110-41568:
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WB
(2)
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Human
(1)
Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 11/29/2016
View

Summary

ApplicationWestern Blot
Sample Testedcardiac tissue
SpeciesMouse

Comments

CommentsProtein was extracted from infarcted tissue by homogenizing the samples sequentially in phosphate buffered saline (PBS) with 1x protease inhibitor cocktail (16 μL per mg tissue, soluble protein fraction), and in protein extraction reagent type 4 (Sigma; 7 M urea, 2 M thiourea, 40 mM Trizma® base and the detergent 1% C7BzO, 15 μL per mg tissue). Immunoblotting was performed with insoluble fraction.
Western Blot LOX propeptide NB110-41568
Enlarge
reviewed by:
Anonymous
WB Human 03/14/2014
View

Summary

ApplicationWestern Blot
Sample Testedsupernatants from human vascular smooth muscle cells
SpeciesHuman
LotB1

Blocking

Blocking Details5% BSA- TBS-tween 0.05%, 1hour, 25º Temperature

Primary Anitbody

Dilution Ratio1:1000 in blocking buffer, overnight, 4º Temperature

Secondary Antibody

Secondary DescriptionPolyclonal Goat anti-rabbit IgG HRP
Secondary Manufacturer Cat#Dako Code No. P 0448
Secondary Concentration1:2000

Details

Detection NotesSuper Signal WestDura, 1minute

Product General Protocols

View specific protocols for LOX propeptide Antibody (NB110-41568): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LOX propeptide Antibody (NB110-41568). (Showing 1 - 2 of 2 FAQs).

  1. Just wondered whether the LOX propeptide Antibody [NB110-41568] has been tested on frozen sections?
    • This antibody has been tested on frozen sections.
  2. Does that mean it has been tested on frozen sections and didn't work?
    • This has been validated for both Paraffin and Frozen. In the applications list we list IHC. This includes both IHC-Frozen and IHC-Paraffin.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-41568AF405
Alexa Fluor 488 Labeled NB110-41568AF488
Alexa Fluor 647 Labeled NB110-41568AF647
Alexa Fluor 700 Labeled NB110-41568AF700
Biotin Labeled NB110-41568B
DyLight 405 Labeled NB110-41568V
DyLight 488 Labeled NB110-41568G
DyLight 550 Labeled NB110-41568R
DyLight 650 Labeled NB110-41568C
FITC Labeled NB110-41568F
HRP Labeled NB110-41568H

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Research Areas for LOX propeptide Antibody (NB110-41568)

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Blogs on LOX propeptide.

LOX propeptide: A novel peptide cancer therapeutic
Lysyl oxidase, also known as LOX, is a copper-dependent enzyme that cross-links collagen and elastin through the oxidative deamination of peptidyl lysine (collagen and elastin) and hydroxylysine (collagen only) residues, thereby playing a critical rol...  Read full blog post.

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Recent Reviews

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Anonymous
11/29/2016
Application: WB
Species: Mouse

Anonymous
03/14/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol LOX
Entrez