Western Blot: LOX propeptide Antibody [NB110-41568] - Detection of LOPP in MC3T3-E1 cell lysate.
Immunohistochemistry-Paraffin: LOX propeptide Antibody [NB110-41568] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse intestine using 1:200 dilution of rabbit anti- LOX propeptide (LOX-PP) ...read more
Western Blot: LOX propeptide Antibody [NB110-41568] - Total protein from human and mouse brain was separated on a 12% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Immunocytochemistry/ Immunofluorescence: LOX propeptide Antibody [NB110-41568] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells ...read more
Western Blot: LOX propeptide Antibody [NB110-41568] - LOX propeptide levels analyzed in supernatants from human vascular smooth muscle cells. Image from verified customer review.
This LOX propeptide antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin and Western Blot where a band is seen at ~18 kDa. The strong band at 50 kDa and smear of bands that run between 46 kDa and 50 kDa are glycosylated and non-glycosylated forms of pro-lysyl oxidase. Bands between 28-36 kDa are glycosylated propeptide. The variation in MW depends on the extent of glycosylation.
Read 2 Reviews rated 3.5 using NB110-41568 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for LOX propeptide Antibody
lysyl oxidaseEC 220.127.116.11
Lysyl oxidase (LOX) is a critical enzyme in extracellular matrix biosynthesis as well as in the maintainance of normal bone phenotype. It is synthesized/secreted as a 50-kD proenzyme 'pro-LOX' which is then processed to 30-kD mature enzyme 'LOX' and 18-kD lysyl oxidase propeptide 'LOX-PP' by extracellular procollagen C-proteinases (BMP1, Tll1, and Tll2). LOX-PP has been proposed to implicate in the maintenance of inactive state of LOX within the secretory pathway and to act as intramolecular chaperones which facilitate the accurate protein folding/targeting to correct destinations. LOX-PP involves tumor suppression by inhibiting RAS-dependent cellular transformation and its expression has also been associated with inhibition of anchorage-independent progression as well as migration and growth suppression xenografts in Her-2/neu-driven breast cancer. LOX-PP also inhibits PI3K/AKT and ERK1/2 MAP kinase pathways, as well as the levels of downstream NF-kB/cyclin D1 signals in certain cancers - breast, pancreatic, lung, prostate and oral cancers. LOX-PP has also been shown to inhibit DNA synthesis in rat's VSMCs cultures, osteoblast proliferation and FGF-2 signaling.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Reviews for LOX propeptide Antibody (NB110-41568) (2)
Average Rating: 3.5 (Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.
Reviews using NB110-41568:
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reviewed by: Anonymous
Protein was extracted from infarcted tissue by homogenizing the samples sequentially in phosphate buffered saline (PBS) with 1x protease inhibitor cocktail (16 μL per mg tissue, soluble protein fraction), and in protein extraction reagent type 4 (Sigma; 7 M urea, 2 M thiourea, 40 mM Trizma® base and the detergent 1% C7BzO, 15 μL per mg tissue). Immunoblotting was performed with insoluble fraction.
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