Vinculin Antibody (hVIN-1)

Images

 
Immunocytochemistry: Vinculin Antibody (hVIN-1) [NB600-1293] - Mouse primary keratinocytes stained with the clone hVIN-1. This image was submitted via customer Review.
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - MCF7 breast carcinoma cells expressing GFP fused CIN85 were labeled at a 1:400 dilution.
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - Primary human osteoblast cells labeled (red), actin was stained with Phalloidin (green) and nuclei stained blue.
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - Double labeling of cultured chicken fibroblasts for vinculin using Monoclonal Anti-Human Vinculin and Rabbit Anti-Tubulin (green).
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - Using confocal microscopy, human skin fibroblasts. The actin filaments were labeled with phalloidin (red) and hyaluronan was stained ...read more
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - A computer-generated micrograph showing the superimposed immunofluorescent staining of chicken lens cells using FITC-phalloidin (green), ...read more
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - Staining of a formaldehyde-fixed section of human myocardium using Monoclonal Anti-Human Vinculin, Clone No. hVIN-1. Nuclei are stained ...read more
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - Human monocytes (J-111) labeled with Monoclonal Anti-Vinculin. Actin filaments (red) and nuclear DNA (blue) were stained with ...read more
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - Primary human osteoblast cells labeled with Anti-Vinculin (red). From Eng San Thian, Materials Science and Metallurgy, University of ...read more
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - Staining of a formaldehyde-fixed section of human myocardium using Monoclonal Anti-Human Vinculin, Clone No. hVIN-1. Nuclei are stained ...read more
Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (hVIN-1) [NB600-1293] - Mouse primary keratinocytes stained with the clone hVIN-1. Image is courtesy of customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Am, Bv, Ca, Ch, TrSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, ICC
Clone
hVIN-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Vinculin Antibody (hVIN-1) Summary

Immunogen
Purified human vinculin from uterus.
Localization
Cytoplasm; cytoskeleton. Cell junction; adherens junction; cytoplasmic side. Cytoplasmic face of adhesion plaques.
Marker
Focal Adhesion Marker
Specificity
Specifically labels vinculin at cell-cell and cell-substrate contacts. Shows cross-reactivity with smooth muscle metavinculin.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VCL
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:400
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunocytochemistry
Application Notes
WB: Detects a band of approximately 116 kDa.
Theoretical MW
116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Vinculin Lysate (NBL1-17706)
Reviewed Applications
Read 1 Review rated 5
using
NB600-1293 in the following applications:

Publications
Read Publications using NB600-1293.

Reactivity Notes

Frog (100%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for Vinculin Antibody (hVIN-1)

  • CMD1W
  • CMH15
  • Metavinculin
  • MVCL
  • VCL
  • Vinculin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Am, Bv, Ca, Ch, Tr
Applications: WB, ICC/IF, IHC, IHC-Fr, ICC

Publications for Vinculin Antibody (NB600-1293)(2)

Review for Vinculin Antibody (NB600-1293) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB600-1293:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Vinculin NB600-1293
Enlarge
reviewed by:
Qi Miao
ICC Mouse 04/26/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedskin keratinocytes
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Vinculin Antibody (NB600-1293). (Showing 1 - 1 of 1 FAQs).

  1. Do you have any 100 coda or bigger controls?
    • I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
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2
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1
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Qi Miao
04/26/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol VCL
Entrez
Uniprot