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MUC2 Antibody (996/1) [DyLight 594]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, Flow-IC, IHC, IHC-Fr, IHC-P
Clone
996/1
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 594

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB120-11197AF350
Alexa Fluor 405
NB120-11197AF405
Alexa Fluor 488
NB120-11197AF488
Alexa Fluor 532
NB120-11197AF532
Alexa Fluor 594
NB120-11197AF594
Alexa Fluor 647
NB120-11197AF647
Alexa Fluor 700
NB120-11197AF700
Alexa Fluor 750
NB120-11197AF750
Allophycocyanin
NB120-11197APC
Allophycocyanin/Cy7
NB120-11197APCCY7
Biotin
NB120-11197B
DyLight 350
NB120-11197UV
DyLight 405
NB120-11197V
DyLight 488
NB120-11197G
DyLight 550
NB120-11197R
DyLight 650
NB120-11197C
DyLight 680
NB120-11197FR
DyLight 755
NB120-11197IR
FITC
NB120-11197F
HRP
NB120-11197H
Janelia Fluor 549
NB120-11197JF549
Janelia Fluor 646
NB120-11197JF646
PE
NB120-11197PE
PE/Atto594
NB120-11197PEATT594
PE/Cy5.5
NB120-11197PECY55
PE/Cy7
NB120-11197PECY7
PerCP
NB120-11197PCP
Unconjugated
NB120-11197
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MUC2 Antibody (996/1) [DyLight 594] Summary

Immunogen
MUC2 tandem repeat peptide
Localization
Secreted
Specificity
NB120-11197 recognizes the human MUC2 mucin, and shows no cross-reactivity with MUC1, MUC3 or MUC4 mucins.In tissue sections NB120-11197 recognizes colon, liver and prostate tissues strongly. The recognizes malignant colonic mucosa and normal mucosa.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MUC2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Flow (Intracellular)
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Mouse reactivity reported in scientific literature (PMID: 24045942).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for MUC2 Antibody (996/1) [DyLight 594]

  • Intestinal mucin-2
  • MLP
  • MUC2
  • MUC-2
  • mucin 2, intestinal/tracheal
  • mucin 2, oligomeric mucus/gel-forming
  • mucin-2
  • SMUC

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MUC1 [SM3] at 5 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: MUC-1 Antibody (SM3) [NB120-22711] - IHC analysis of formalin fixed paraffin embedded tissue section of human breast cancer xenograft using MUC-1 antibody clone SM3 at 1:10 dilution. The xenograft section depicted a very specific and intense signal in the periphery of the cancer cells. The necrotic cells also developed a strong immune-positivity while the tumor stroma as well as the nuclei of cells were negative for immunostaining.
Immunocytochemistry/Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MUC-1 antibody was tested in MCF7 cells at 10 ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.
(1 Review)
(11 Publications)
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
MUC5AC Antibody
(45M1)
NBP2-15196
Western Blot: MUC5AC Antibody (45M1) [NBP2-15196] - AGS human gastric adenocarcinoma and KATO-III human gastric carcinoma cell line lysates. Image from verified customer review.
Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (45M1).
Flow Cytometry: MUC5AC Antibody (45M1) [NBP2-15196] - An intracellular stain was performed on A549 cells with Mucin 5AC (45M1) antibody NBP2-15196PE (blue) and a matched isotype control NBP2-27287PE (orange). To block the secretion of Mucin 5AC, cells were treated with 3uM Monensin for 3 hours. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both Antibodies were conjugated to Phycoerythrin.
(4 Reviews)
(11 Publications)
Species: Hu, Mu, Rt, Po, Ch, Fe, Pm, Rb, Bv(-)
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC
MUC6 Antibody
(MUC6/916)
NBP2-44376
Immunohistochemistry-Paraffin: MUC6 Antibody (MUC6/916) [NBP2-44376] - Human Gastric Carcinoma stained with MUC6 Monoclonal Antibody (MUC6/916).
Immunohistochemistry-Paraffin: MUC6 Antibody (MUC6/916) [NBP2-44376] - Human Gastric Carcinoma stained with MUC6 Monoclonal Antibody (MUC6/916).
Species: Hu, Rt(-)
Applications: Flow, IHC, IHC-P, IF
MUC5B Antibody
NBP1-92151
Immunocytochemistry/Immunofluorescence: MUC5B Antibody [NBP1-92151] - Staining of human cell line A549 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: MUC5B Antibody [NBP1-92151] - Analysis in human cervix, uterine and skeletal muscle tissues. Corresponding MUC5B RNA-seq data are presented for the same tissues.
(3 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
MUC4 Antibody
NBP1-52193
Western Blot: MUC4 Antibody [NBP1-52193] - MUC4 is shown in the green channel, with beta-tubulin in red channel and Precision Plus dual color protein ladder in the left. The main band is about 60 kDa, with another band about 40 kDa. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: MUC4 Antibody [NBP1-52193] - Staining of MUC4 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: MUC4 Antibody [NBP1-52193] - Staining of MUC4 in mouse prostate
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(2 Publications)
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Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Cytokeratin 20
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NBP2-67309
Western Blot: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Analysis of Cytokeratin 20 on different cell lysates using anti-Cytokeratin 20 antibody at 1/1,000 dilution. Positive control: Lane 1: Lovo Lane 2: HCT116 Lane 3: CRC
Immunocytochemistry/Immunofluorescence: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Staining Cytokeratin 20 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Analysis of paraffin-embedded rat small intestine tissue using anti-Cytokeratin 20 antibody. Counter stained with hematoxylin.
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
MUC3 Antibody
(M3.1) - IHC-Predilut
NBP2-48392
Immunohistochemistry-Paraffin: MUC3 Antibody (M3.1) - IHC-Prediluted [NBP2-48392] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC3 Mouse Monoclonal Antibody (M3.1).
Immunohistochemistry-Paraffin: MUC3 Antibody (M3.1) - IHC-Prediluted [NBP2-48392] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC3 Mouse Monoclonal Antibody (M3.1).
Immunohistochemistry-Paraffin: MUC3 Antibody (M3.1) - IHC-Prediluted [NBP2-48392] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC3 Mouse Monoclonal Antibody (M3.1).
Species: Hu
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CDX2 Antibody
NB100-2136
Immunohistochemistry-Paraffin: CDX2 Antibody [NB100-2136] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CDX2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: CDX2 Antibody [NB100-2136] - Detection of Human CDX2 on HeLa whole cell lysate using NB100-2136. CDX2 was also immunoprecipitated by rabbit anti-CDX2 antibody NB100-2137 using 3 mcg/mg lysate.
(1 Review)
(9 Publications)
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Applications: WB (-), IHC, IHC-P, IP
Neprilysin/CD10
Antibody (SN75-07)
NBP2-67717
Western Blot: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of CD10 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Staining CD10 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of paraffin-embedded human tonsil tissue using anti-CD10 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
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Cytokeratin 7
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NBP2-47940
Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).
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p53 Antibody (PAb
240)
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1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
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TFF3 Antibody (3D9)
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Immunohistochemistry-Paraffin: TFF3 Antibody (3D9) [H00007033-M01] - Analysis of monoclonal antibody to TFF3 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
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CSRP3 Antibody
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Western Blot: CSRP3 Antibody [NBP1-06983] - (0.01ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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MUC7 Antibody
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MUC7 Antibody (4D2-1D7)
(1 Publication)
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Applications: WB, ELISA, IHC, PAGE
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Western Blot: PDLIM5 Antibody [NBP1-84843] - Analysis using Anti-PDLIM5 antibody NBP1-84843 (A) shows similar pattern to independent antibody NBP1-84842 (B).
Immunocytochemistry/Immunofluorescence: PDLIM5 Antibody [NBP1-84843] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & focal adhesion sites.
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EGFR Antibody
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Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
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MUC2 Antibody
(996/1) [DyLight 594]
NB120-11197DL594
MUC2 Antibody (996/1) [DyLight 594]
Species: Hu, Mu
Applications: WB, Flow, Flow-IC, IHC, IHC-Fr, IHC-P

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB120-11197AF350
Alexa Fluor 405 NB120-11197AF405
Alexa Fluor 488 NB120-11197AF488
Alexa Fluor 532 NB120-11197AF532
Alexa Fluor 594 NB120-11197AF594
Alexa Fluor 647 NB120-11197AF647
Alexa Fluor 700 NB120-11197AF700
Alexa Fluor 750 NB120-11197AF750
Allophycocyanin NB120-11197APC
Allophycocyanin/Cy7 NB120-11197APCCY7
Biotin NB120-11197B
DyLight 350 NB120-11197UV
DyLight 405 NB120-11197V
DyLight 488 NB120-11197G
DyLight 550 NB120-11197R
DyLight 594 NB120-11197DL594
DyLight 650 NB120-11197C
DyLight 680 NB120-11197FR
DyLight 755 NB120-11197IR
FITC NB120-11197F
HRP NB120-11197H
Janelia Fluor 549 NB120-11197JF549
Janelia Fluor 646 NB120-11197JF646
PE NB120-11197PE
PE/Atto594 NB120-11197PEATT594
PE/Cy5.5 NB120-11197PECY55
PE/Cy7 NB120-11197PECY7
PerCP NB120-11197PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol MUC2