MUC2 Antibody (996/1)

Images

 
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody [996/1] NB120-11197AF647 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Permeabilised HL-60 cells stained with Mouse anti Human MUC-2:FITC
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Analysis of Allophycocyanin conjugate of NB120-11197. An intracellular stain was performed on HeLa cells with MUC2 (996/1) antibody NB120-11197APC (blue) and a ...read more
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Analysis of Allophycocyanin conjugate of NB120-11197. An intracellular stain was performed on NTERA-2 cells with MUC2 (996/1) antibody NB120-11197APC (blue) and a ...read more
Flow (Intracellular): MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody (996/1) NB120-11197AF488 (blue) and a matched isotype control (orange). Cells were fixed ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, Flow-IC, IHC, IHC-Fr, IHC-P
Clone
996/1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MUC2 Antibody (996/1) Summary

Immunogen
MUC2 tandem repeat peptide
Localization
Secreted
Specificity
NB120-11197 recognizes the human MUC2 mucin, and shows no cross-reactivity with MUC1, MUC3 or MUC4 mucins.In tissue sections NB120-11197 recognizes colon, liver and prostate tissues strongly. The recognizes malignant colonic mucosa and normal mucosa.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MUC2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Flow (Intracellular)
Application Notes
Membrane permeabilization is required for Flow Cytometry. Mouse reactivity reported in scientific literature (PMID: 24045942)
Control
Colon Lysate (NB820-59205)
Reviewed Applications
Read 1 Review rated 3
using
NB120-11197 in the following applications:

Publications
Read Publication using
NB120-11197 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for MUC2 Antibody (996/1)

  • Intestinal mucin-2
  • MLP
  • MUC-2
  • mucin 2, intestinal/tracheal
  • mucin 2, oligomeric mucus/gel-forming
  • mucin-2
  • mucin-like protein
  • SMUC

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MUC2 Antibody (NB120-11197)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-Fr.


Filter By Application
IHC-Fr
(1)
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Filter By Species
Mouse
(1)
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Review for MUC2 Antibody (NB120-11197) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB120-11197:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Other 03/11/2009
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedpig lung
SpeciesOther
Lot996/1
CommentsDid not work on pig as we were hoping.

Blocking

Blocking DetailsBlocking Buffer: background buster, Blocking Time: 30 minutes, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: several, Incubation Dilution Buffer: DAKO 1x buffer, Incubation Time: several, Incubation Temp: RT

Details

Detection NotesAntigen Retrieval: PK citrate buffer EDTA microwave steamer

Comments

CommentsDid not work on pig as we were hoping.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MUC2 Antibody (NB120-11197) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB120-11197AF405
Alexa Fluor 488 Labeled NB120-11197AF488
Alexa Fluor 647 Labeled NB120-11197AF647
Alexa Fluor 700 Labeled NB120-11197AF700
Allophycocyanin Labeled NB120-11197APC
DyLight 350 Labeled NB120-11197UV
DyLight 405 Labeled NB120-11197V
DyLight 488 Labeled NB120-11197G
DyLight 680 Labeled NB120-11197FR
DyLight 755 Labeled NB120-11197IR
PE Labeled NB120-11197PE
PerCP Labeled NB120-11197PCP

Additional Array Products

Array NB120-11197

Bioinformatics Tool for MUC2 Antibody (NB120-11197)

Discover related pathways, diseases and genes to MUC2 Antibody (NB120-11197). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for MUC2 Antibody (NB120-11197)

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Blogs on MUC2

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Recent Reviews

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Anonymous
03/11/2009
Application: IHC-P
Species: Other

Bioinformatics

Gene Symbol MUC2
Entrez
Uniprot