MUC2 Antibody (996/1)

Images

 
Immunocytochemistry/ Immunofluorescence: MUC2 Antibody (996/1) [NB120-11197] - MS-induced intestinal epithelium injury was CRHR1 dependent.Photomicrographs of immunofluorescence of Mucin 2 (Muc2; mucous-forming protein) ...read more
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody [996/1] NB120-11197AF647 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Immunocytochemistry/ Immunofluorescence: MUC2 Antibody (996/1) [NB120-11197] - MS-induced intestinal epithelium injury was CRHR1 dependent. Photomicrographs of immunofluorescence of Mucin 2 (Muc2; mucous-forming ...read more
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Permeabilised HL-60 cells stained with Mouse anti Human MUC-2:FITC
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Analysis of Allophycocyanin conjugate of NB120-11197. An intracellular stain was performed on HeLa cells with MUC2 (996/1) antibody NB120-11197APC (blue) and a ...read more
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Analysis of Allophycocyanin conjugate of NB120-11197. An intracellular stain was performed on NTERA-2 cells with MUC2 (996/1) antibody NB120-11197APC (blue) and a ...read more
Flow (Intracellular): MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody (996/1) NB120-11197AF488 (blue) and a matched isotype control (orange). Cells were fixed ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
996/1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

MUC2 Antibody (996/1) Summary

Immunogen
MUC2 tandem repeat peptide
Localization
Secreted
Specificity
NB120-11197 recognizes the human MUC2 mucin, and shows no cross-reactivity with MUC1, MUC3 or MUC4 mucins.In tissue sections NB120-11197 recognizes colon, liver and prostate tissues strongly. The recognizes malignant colonic mucosa and normal mucosa.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MUC2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Flow (Intracellular)
Application Notes
Membrane permeabilization is required for Flow Cytometry.
Control
Human Colon Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 1 Review rated 3
using
NB120-11197 in the following applications:

Publications
Read Publications using
NB120-11197 in the following applications:

  • IHC
    2 publications
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Mouse reactivity reported in scientific literature (PMID: 24045942).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for MUC2 Antibody (996/1)

  • Intestinal mucin-2
  • MLP
  • MUC2
  • MUC-2
  • mucin 2, intestinal/tracheal
  • mucin 2, oligomeric mucus/gel-forming
  • mucin-2
  • SMUC

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB120-11197
Species: Hu, Mu
Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for MUC2 Antibody (NB120-11197)(5)

Review for MUC2 Antibody (NB120-11197) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB120-11197:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
  3
reviewed by:
Anonymous
IHC-P Other 03/11/2009
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedpig lung
SpeciesOther
Lot996/1
CommentsDid not work on pig as we were hoping.

Blocking

Blocking DetailsBlocking Buffer: background buster, Blocking Time: 30 minutes, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: several, Incubation Dilution Buffer: DAKO 1x buffer, Incubation Time: several, Incubation Temp: RT

Details

Detection NotesAntigen Retrieval: PK citrate buffer EDTA microwave steamer

Comments

CommentsDid not work on pig as we were hoping.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MUC2 Antibody (NB120-11197) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB120-11197AF350
Alexa Fluor 405 NB120-11197AF405
Alexa Fluor 488 NB120-11197AF488
Alexa Fluor 532 NB120-11197AF532
Alexa Fluor 594 NB120-11197AF594
Alexa Fluor 647 NB120-11197AF647
Alexa Fluor 700 NB120-11197AF700
Alexa Fluor 750 NB120-11197AF750
Allophycocyanin NB120-11197APC
Allophycocyanin/Cy7 NB120-11197APCCY7
Biotin NB120-11197B
DyLight 350 NB120-11197UV
DyLight 405 NB120-11197V
DyLight 488 NB120-11197G
DyLight 550 NB120-11197R
DyLight 594 NB120-11197DL594
DyLight 650 NB120-11197C
DyLight 680 NB120-11197FR
DyLight 755 NB120-11197IR
FITC NB120-11197F
HRP NB120-11197H
Janelia Fluor 549 NB120-11197JF549
Janelia Fluor 646 NB120-11197JF646
PE NB120-11197PE
PE/Atto594 NB120-11197PEATT594
PE/Cy5.5 NB120-11197PECY55
PE/Cy7 NB120-11197PECY7
PerCP NB120-11197PCP

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Recent Reviews

3
5
0
4
0
3
1
2
0
1
0

 
Anonymous
03/11/2009
Application: IHC-P
Species: Other

Bioinformatics

Gene Symbol MUC2
Entrez
Uniprot