MUC2 Antibody (996/1) - BSA Free

Immunohistochemistry: MUC2 Antibody (996/1) [NB120-11197] - Immunofluorescence of Mucin 2 (Muc2; mucous-forming protein) (F-J) in proximal colon in all experimental groups. Histological scores were highest in MS, demonstrated injury in MS compared to control. Treatment with Antalarmin and Astressin prevented this MS-induced colonic injury, but not by Astressin-2B. Image collected and cropped by CiteAb from the following publication (nature.com/articles/srep46616), licensed under a CC-BY licence.

Immunohistochemistry: MUC2 Antibody (996/1) [NB120-11197] - Maternal LB modulates IL-1B-induced goblet cell Tff3 and MUC2 response to inflammatory stress in pre-weaned mice. a,b) Immunofluorescence detection of MUC2 molecules (red) in the mouse goblet cells in SPF (a) vs SPF/LB pups (b). Representative areas (n = 3 per group) are shown Bar 100 uM, magnification 400x. c,d) Representative areas of MUC2 exclusion from goblet cells in response to IL-1B stimulation in SPF (c) or SPF/LB (d) pups are shown; magnification 400x. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0237182), licensed under a CC-BY licence.

Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody [996/1] NB120-11197AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Analysis of Allophycocyanin conjugate of NB120-11197. An intracellular stain was performed on HeLa cells with MUC2 (996/1) antibody NB120-11197APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were fixed with 4% PFA and then permeabli

Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Analysis of Allophycocyanin conjugate of NB120-11197. An intracellular stain was performed on NTERA-2 cells with MUC2 (996/1) antibody NB120-11197APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were fixed with 4% PFA and then permea

Flow (Intracellular): MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody (996/1) NB120-11197AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

• Reactivity: Hu, Mu
• Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: 996/1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MUC2 Antibody (996/1) - BSA Free Summary

• Immunogen: This MUC2 Antibody (996/1) was developed against MUC2 tandem repeat peptide
• Localization: Secreted
• Specificity: MUC2 Antibody (996/1) recognizes the human MUC2 mucin, and shows no cross-reactivity with MUC1, MUC3 or MUC4 mucins. In tissue sections colon, liver and prostate tissues strongly. It recognizes malignant colonic mucosa and normal mucosa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MUC2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Membrane permeabilization is required for Flow Cytometry.
• Theoretical MW: 540 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Colon Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Mouse reactivity reported in scientific literature (PMID: 24045942).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for MUC2 Antibody (996/1) - BSA Free

• Intestinal mucin-2
• MLP
• MUC2
• MUC-2
• mucin 2, intestinal/tracheal
• mucin 2, oligomeric mucus/gel-forming
• mucin-2
• SMUC

Background

MUC2, also called mucin 2, is a member of the mucin protein family which are heavily glycosylated, high molecular weight proteins that are produced by goblet cells in epithelial tissues including the intestinal and respiratory tract (1). Mucins can form gels and function in lubrication of mucosal surfaces which serve as a protective barrier of the epithelial lining and an innate host defense (1). Mucins are divided into two groups based on whether they are membrane-bound or secreted, where MUC2 belongs to the secretory group (1,2). Secreted mucins have a distinctive composition characterized by a large central exon "mucin domain" containing O-glycosylated tandem repeat regions rich in proline, threonine, and serine residues, non-repetitive domains, and cysteine-rich domains (1,2). The mucin domain is flanked by 5' and 3' D domains, a B-like domain, a C-like domain, and a Ck-domain (1,2). MUC2 was the first secretory mucin identified in humans (3,4). Full-length MUC2 protein is synthesized as 5179 amino acids (aa) in length with a theoretical molecular weight of ~540 kDa (5).

Changes or perturbations to MUC2 expression has been associated with a number of disease pathologies (1-4, 6). Specifically, altered MUC2 composition has been indicated in colorectal cancer, inflammatory bowel diseases (IBD) including ulcerative colitis and Chron's disease, and chronic obstructive pulmonary disease (COPD) (1-4, 6). In general, decreased or reduced MUC2 expression is associated with colorectal cancer disease progression and development of IBD (1-4, 6). Additionally, studies have found that upon intestinal infection due to parasites or bacteria MUC2 expression is increased, as is overall mucus secretion (3, 6).

References

1. Pothuraju, R., Krishn, S. R., Gautam, S. K., Pai, P., Ganguly, K., Chaudhary, S., Rachagani, S., Kaur, S., & Batra, S. K. (2020). Mechanistic and Functional Shades of Mucins and Associated Glycans in Colon Cancer. Cancers. https://doi.org/10.3390/cancers12030649

2. Ballester, B., Milara, J., & Cortijo, J. (2019). Mucins as a New Frontier in Pulmonary Fibrosis. Journal of Clinical Medicine. https://doi.org/10.3390/jcm8091447

3. Liu, Y., Yu, X., Zhao, J., Zhang, H., Zhai, Q., & Chen, W. (2020). The role of MUC2 mucin in intestinal homeostasis and the impact of dietary components on MUC2 expression. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2020.07.191

4. Allen, A., Hutton, D. A., & Pearson, J. P. (1998). The MUC2 gene product: a human intestinal mucin. The International Journal of Biochemistry & Cell Biology. https://doi.org/10.1016/s1357-2725(98)00028-4

5. Uniprot (Q02817)

6. Kim, Y. S., & Ho, S. B. (2010). Intestinal goblet cells and mucins in health and disease: recent insights and progress. Current Gastroenterology Reports. https://doi.org/10.1007/s11894-010-0131-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MUC2 Antibody (NB120-11197)(10)

Publications using NB120-11197

• Wanner N, Barnhart J, Apostolakis N Et al. Using the Autofluorescence Finder on the Sony ID7000(TM) Spectral Cell Analyzer to Identify and Unmix Multiple Highly Autofluorescent Murine Lung Populations Front Bioeng Biotechnol 2022-04-04 [PMID: 35372303] (FLOW, Mouse)
  
  Details:
  Citation using the Alexa Fluor 532 version of this antibody.
• Bao L, Cui X, Wang X et al. Carbon Nanotubes Promote the Development of Intestinal Organoids through Regulating Extracellular Matrix Viscoelasticity and Intracellular Energy Metabolism ACS Nano 2021-10-26 [PMID: 34622660]
  
  Details:
  Citation using the PE format of this antibody.
• Xu P, Xi Y, Zhu J et al. Intestinal Sulfation Is Essential to Protect Against Colitis and Colonic Carcinogenesis Gastroenterology 2021-04-02 [PMID: 33819483] (IHC, Mouse)
• Yang KS, Ciprani D, O'Shea A et al. EXTRACELLULAR VESICLE ANALYSIS ALLOWS FOR IDENTIFICATION OF INVASIVE IPMN Gastroenterology 2020-12-07 [PMID: 33301777] (Human)
• Wu H, Chen QY, Wang WZ et al. Compound sophorae decoction enhances intestinal barrier function of dextran sodium sulfate induced colitis via regulating notch signaling pathway in mice Biomed Pharmacother 2020-11-17 [PMID: 33217689] (WB, Mouse)
• Yu Y, Lu J, Oliphant K et al. Maternal administration of probiotics promotes gut development in mouse offsprings PLoS ONE 2020-08-07 [PMID: 32764797] (Mouse)
• Talbot S, Doyle B, et al. Vagal sensory neurons drive mucous cell metaplasia. J Allergy Clin Immunol 2020-06-01 [PMID: 31954778] (FLOW, Mouse)
• Li B, Lee C et al. Inhibition of corticotropin-releasing hormone receptor 1 and activation of receptor 2 protect against colonic injury and promote epithelium repair. Sci Rep 2017-11-05 [PMID: 31748698] (IHC, Mouse)
• Li B, Lee C, Filler T, Hock A Inhibition of corticotropin-releasing hormone receptor 1 and activation of receptor 2 protect against colonic injury and promote epithelium repair Sci Rep. 2017-05-10 [PMID: 28492284] (IHC, Mouse)
• Mishra J, Verma RK, Alpini G et al. Role of janus kinase 3 in mucosal differentiation and predisposition to colitis. J Biol Chem. 2013-11-01 [PMID: 24045942] (IHC-Fr, Mouse)

Review for MUC2 Antibody (NB120-11197) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: System Admin IHC-P Other 03/11/2009

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: pig lung
• Species: Other
• Lot: 996/1

Bioinformatics

• Gene Symbol: MUC2
• Entrez:
  • 4583(Human)
• Uniprot:
  • Q02817(Human)