MUC2 Antibody (996/1) - BSA Free

Images

 
Biological Strategies: Immunohistochemistry: MUC2 Antibody (996/1) [NB120-11197] - Immunofluorescence of Mucin 2 (Muc2; mucous-forming protein) (F-J) in proximal colon in all experimental groups. Histological ...read more
Immunohistochemistry: MUC2 Antibody (996/1) [NB120-11197] - Maternal LB modulates IL-1B-induced goblet cell Tff3 and MUC2 response to inflammatory stress in pre-weaned mice. a,b) Immunofluorescence detection of MUC2 ...read more
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody [996/1] NB120-11197AF647 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Analysis of Allophycocyanin conjugate of NB120-11197. An intracellular stain was performed on HeLa cells with MUC2 (996/1) antibody NB120-11197APC (blue) and a ...read more
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Analysis of Allophycocyanin conjugate of NB120-11197. An intracellular stain was performed on NTERA-2 cells with MUC2 (996/1) antibody NB120-11197APC (blue) and a ...read more
Flow (Intracellular): MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody (996/1) NB120-11197AF488 (blue) and a matched isotype control (orange). Cells were fixed ...read more
Characterization of polyclonal antibodies directed to human rab31. (A) The reaction pattern of polyclonal antibody (pAb) RT3-IgG (black) and of the pre-immune rabbit serum (gray) was analyzed by a ‘one-sided ELISA’ ...read more
GAS6 increases the amount of both CIP2A and p-DAPK in RSC96 cells(A) RSC96 cells were incubated with GAS6 (100 ng/ml), and immunoblotting evaluations of CIP2A and p-DAPK expression were performed. The lower panel shows ...read more
Ddx19 interacts with MKL1 RPEL domain and Ipo beta .(a) Ddx19 recruitment from Hela cell lysate by GST-RPEL and GST-Ipo beta (left). WB, western blotting; (−), without Ran-Q69L; (+), with RanQ69L. Corresponding GST ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
996/1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

MUC2 Antibody (996/1) - BSA Free Summary

Immunogen
This MUC2 Antibody (996/1) was developed against MUC2 tandem repeat peptide
Localization
Secreted
Specificity
MUC2 Antibody (996/1) recognizes the human MUC2 mucin, and shows no cross-reactivity with MUC1, MUC3 or MUC4 mucins. In tissue sections colon, liver and prostate stain strongly. It recognizes malignant colonic mucosa and normal mucosa.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MUC2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
Membrane permeabilization is required for Flow Cytometry.
Theoretical MW
540 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Colon Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 1 Review rated 3
using
NB120-11197 in the following applications:

Publications
Read Publications using
NB120-11197 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Mouse reactivity reported in scientific literature (PMID: 24045942).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for MUC2 Antibody (996/1) - BSA Free

  • Intestinal mucin-2
  • MLP
  • MUC2
  • MUC-2
  • mucin 2, intestinal/tracheal
  • mucin 2, oligomeric mucus/gel-forming
  • mucin-2
  • SMUC

Background

MUC2, also called mucin 2, is a member of the mucin protein family which are heavily glycosylated, high molecular weight proteins that are produced by goblet cells in epithelial tissues including the intestinal and respiratory tract (1). Mucins can form gels and function in lubrication of mucosal surfaces which serve as a protective barrier of the epithelial lining and an innate host defense (1). Mucins are divided into two groups based on whether they are membrane-bound or secreted, where MUC2 belongs to the secretory group (1,2). Secreted mucins have a distinctive composition characterized by a large central exon "mucin domain" containing O-glycosylated tandem repeat regions rich in proline, threonine, and serine residues, non-repetitive domains, and cysteine-rich domains (1,2). The mucin domain is flanked by 5' and 3' D domains, a B-like domain, a C-like domain, and a Ck-domain (1,2). MUC2 was the first secretory mucin identified in humans (3,4). Full-length MUC2 protein is synthesized as 5179 amino acids (aa) in length with a theoretical molecular weight of ~540 kDa (5).

Changes or perturbations to MUC2 expression has been associated with a number of disease pathologies (1-4, 6). Specifically, altered MUC2 composition has been indicated in colorectal cancer, inflammatory bowel diseases (IBD) including ulcerative colitis and Chron's disease, and chronic obstructive pulmonary disease (COPD) (1-4, 6). In general, decreased or reduced MUC2 expression is associated with colorectal cancer disease progression and development of IBD (1-4, 6). Additionally, studies have found that upon intestinal infection due to parasites or bacteria MUC2 expression is increased, as is overall mucus secretion (3, 6).

References

1. Pothuraju, R., Krishn, S. R., Gautam, S. K., Pai, P., Ganguly, K., Chaudhary, S., Rachagani, S., Kaur, S., & Batra, S. K. (2020). Mechanistic and Functional Shades of Mucins and Associated Glycans in Colon Cancer. Cancers. https://doi.org/10.3390/cancers12030649

2. Ballester, B., Milara, J., & Cortijo, J. (2019). Mucins as a New Frontier in Pulmonary Fibrosis. Journal of Clinical Medicine. https://doi.org/10.3390/jcm8091447

3. Liu, Y., Yu, X., Zhao, J., Zhang, H., Zhai, Q., & Chen, W. (2020). The role of MUC2 mucin in intestinal homeostasis and the impact of dietary components on MUC2 expression. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2020.07.191

4. Allen, A., Hutton, D. A., & Pearson, J. P. (1998). The MUC2 gene product: a human intestinal mucin. The International Journal of Biochemistry & Cell Biology. https://doi.org/10.1016/s1357-2725(98)00028-4

5. Uniprot (Q02817)

6. Kim, Y. S., & Ho, S. B. (2010). Intestinal goblet cells and mucins in health and disease: recent insights and progress. Current Gastroenterology Reports. https://doi.org/10.1007/s11894-010-0131-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MUC2 Antibody (NB120-11197)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: FLOW, IF/IHC, IHC-Fr, WB.


Filter By Application
FLOW
(2)
IF/IHC
(3)
IHC-Fr
(1)
WB
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(8)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB120-11197 Applications Species
Xie Z, Li M, Qian M et al. Co-Cultures of Lactobacillus acidophilus and Bacillus subtilis Enhance Mucosal Barrier by Modulating Gut Microbiota-Derived Short-Chain Fatty Acids Nutrients 2022-10-25 [PMID: 36364738] (WB) WB
Wanner N, Barnhart J, Apostolakis N Et al. Using the Autofluorescence Finder on the Sony ID7000(TM) Spectral Cell Analyzer to Identify and Unmix Multiple Highly Autofluorescent Murine Lung Populations Front Bioeng Biotechnol 2022-04-04 [PMID: 35372303] (FLOW, Mouse)

Details:
Citation using the Alexa Fluor 532 version of this antibody.
FLOW Mouse
Bao L, Cui X, Wang X et al. Carbon Nanotubes Promote the Development of Intestinal Organoids through Regulating Extracellular Matrix Viscoelasticity and Intracellular Energy Metabolism ACS Nano 2021-10-26 [PMID: 34622660]

Details:
Citation using the PE format of this antibody.
Xu P, Xi Y, Zhu J et al. Intestinal Sulfation Is Essential to Protect Against Colitis and Colonic Carcinogenesis Gastroenterology 2021-04-02 [PMID: 33819483] (IF/IHC, Mouse) IF/IHC Mouse
Yang KS, Ciprani D, O'Shea A et al. EXTRACELLULAR VESICLE ANALYSIS ALLOWS FOR IDENTIFICATION OF INVASIVE IPMN Gastroenterology 2020-12-07 [PMID: 33301777] (Human) Human
Wu H, Chen QY, Wang WZ et al. Compound sophorae decoction enhances intestinal barrier function of dextran sodium sulfate induced colitis via regulating notch signaling pathway in mice Biomed Pharmacother 2020-11-17 [PMID: 33217689] (WB, Mouse) WB Mouse
Yu Y, Lu J, Oliphant K et al. Maternal administration of probiotics promotes gut development in mouse offsprings PLoS ONE 2020-08-07 [PMID: 32764797] (Mouse) Mouse
Talbot S, Doyle B, et al. Vagal sensory neurons drive mucous cell metaplasia. J Allergy Clin Immunol 2020-06-01 [PMID: 31954778] (FLOW, Mouse) FLOW Mouse
Li B, Lee C et al. Inhibition of corticotropin-releasing hormone receptor 1 and activation of receptor 2 protect against colonic injury and promote epithelium repair. Sci Rep 2017-11-05 [PMID: 31748698] (IF/IHC, Mouse) IF/IHC Mouse
Li B, Lee C, Filler T, Hock A Inhibition of corticotropin-releasing hormone receptor 1 and activation of receptor 2 protect against colonic injury and promote epithelium repair Sci Rep. 2017-05-10 [PMID: 28492284] (IF/IHC, Mouse) IF/IHC Mouse
Show All 11 Publications.

Review for MUC2 Antibody (NB120-11197) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB120-11197:
Filter by Applications
IHC-P
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
  3
reviewed by:
Verified Customer
IHC-P Other 03/11/2009
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedpig lung
SpeciesOther
Lot996/1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MUC2 Antibody (NB120-11197) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional MUC2 Products

Array NB120-11197

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Verified Customer
03/11/2009
Application: IHC-P
Species: Other

Bioinformatics

Gene Symbol MUC2
Entrez
Uniprot