MUC2 Antibody (996/1)

Images

 
Biological Strategies: Immunohistochemistry: MUC2 Antibody (996/1) [NB120-11197] - Immunofluorescence of Mucin 2 (Muc2; mucous-forming protein) (F-J) in proximal colon in all experimental groups. Histological ...read more
Immunohistochemistry: MUC2 Antibody (996/1) [NB120-11197] - Maternal LB modulates IL-1B-induced goblet cell Tff3 and MUC2 response to inflammatory stress in pre-weaned mice. a,b) Immunofluorescence detection of MUC2 ...read more
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody [996/1] NB120-11197AF647 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Analysis of Allophycocyanin conjugate of NB120-11197. An intracellular stain was performed on HeLa cells with MUC2 (996/1) antibody NB120-11197APC (blue) and a ...read more
Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Analysis of Allophycocyanin conjugate of NB120-11197. An intracellular stain was performed on NTERA-2 cells with MUC2 (996/1) antibody NB120-11197APC (blue) and a ...read more
Flow (Intracellular): MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody (996/1) NB120-11197AF488 (blue) and a matched isotype control (orange). Cells were fixed ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
996/1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

MUC2 Antibody (996/1) Summary

Immunogen
This MUC2 Antibody (996/1) was developed against MUC2 tandem repeat peptide
Localization
Secreted
Specificity
MUC2 Antibody (996/1) recognizes the human MUC2 mucin, and shows no cross-reactivity with MUC1, MUC3 or MUC4 mucins. In tissue sections colon, liver and prostate tissues strongly. It recognizes malignant colonic mucosa and normal mucosa.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MUC2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
Membrane permeabilization is required for Flow Cytometry.
Theoretical MW
540 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Colon Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 1 Review rated 3
using
NB120-11197 in the following applications:

Publications
Read Publications using
NB120-11197 in the following applications:

  • IHC
    3 publications
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Mouse reactivity reported in scientific literature (PMID: 24045942).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for MUC2 Antibody (996/1)

  • Intestinal mucin-2
  • MLP
  • MUC2
  • MUC-2
  • mucin 2, intestinal/tracheal
  • mucin 2, oligomeric mucus/gel-forming
  • mucin-2
  • SMUC

Background

MUC2, also called mucin 2, is a member of the mucin protein family which are heavily glycosylated, high molecular weight proteins that are produced by goblet cells in epithelial tissues including the intestinal and respiratory tract (1). Mucins can form gels and function in lubrication of mucosal surfaces which serve as a protective barrier of the epithelial lining and an innate host defense (1). Mucins are divided into two groups based on whether they are membrane-bound or secreted, where MUC2 belongs to the secretory group (1,2). Secreted mucins have a distinctive composition characterized by a large central exon "mucin domain" containing O-glycosylated tandem repeat regions rich in proline, threonine, and serine residues, non-repetitive domains, and cysteine-rich domains (1,2). The mucin domain is flanked by 5' and 3' D domains, a B-like domain, a C-like domain, and a Ck-domain (1,2). MUC2 was the first secretory mucin identified in humans (3,4). Full-length MUC2 protein is synthesized as 5179 amino acids (aa) in length with a theoretical molecular weight of ~540 kDa (5).

Changes or perturbations to MUC2 expression has been associated with a number of disease pathologies (1-4, 6). Specifically, altered MUC2 composition has been indicated in colorectal cancer, inflammatory bowel diseases (IBD) including ulcerative colitis and Chron's disease, and chronic obstructive pulmonary disease (COPD) (1-4, 6). In general, decreased or reduced MUC2 expression is associated with colorectal cancer disease progression and development of IBD (1-4, 6). Additionally, studies have found that upon intestinal infection due to parasites or bacteria MUC2 expression is increased, as is overall mucus secretion (3, 6).

References

1. Pothuraju, R., Krishn, S. R., Gautam, S. K., Pai, P., Ganguly, K., Chaudhary, S., Rachagani, S., Kaur, S., & Batra, S. K. (2020). Mechanistic and Functional Shades of Mucins and Associated Glycans in Colon Cancer. Cancers. https://doi.org/10.3390/cancers12030649

2. Ballester, B., Milara, J., & Cortijo, J. (2019). Mucins as a New Frontier in Pulmonary Fibrosis. Journal of Clinical Medicine. https://doi.org/10.3390/jcm8091447

3. Liu, Y., Yu, X., Zhao, J., Zhang, H., Zhai, Q., & Chen, W. (2020). The role of MUC2 mucin in intestinal homeostasis and the impact of dietary components on MUC2 expression. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2020.07.191

4. Allen, A., Hutton, D. A., & Pearson, J. P. (1998). The MUC2 gene product: a human intestinal mucin. The International Journal of Biochemistry & Cell Biology. https://doi.org/10.1016/s1357-2725(98)00028-4

5. Uniprot (Q02817)

6. Kim, Y. S., & Ho, S. B. (2010). Intestinal goblet cells and mucins in health and disease: recent insights and progress. Current Gastroenterology Reports. https://doi.org/10.1007/s11894-010-0131-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB120-11197
Species: Hu, Mu
Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for MUC2 Antibody (NB120-11197)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IHC-Fr, WB.


Filter By Application
IHC
(3)
IHC-Fr
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(6)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB120-11197 Applications Species
Xu P, Xi Y, Zhu J et al. Intestinal Sulfation Is Essential to Protect Against Colitis and Colonic Carcinogenesis Gastroenterology Apr 2 2021 [PMID: 33819483] (IHC, Mouse)

Details:
Immunohistochemistry was performed using the MUC2 antibody on tissue from the intestinal track of mice.
IHC Mouse
Yang KS, Ciprani D, O'Shea A et al. EXTRACELLULAR VESICLE ANALYSIS ALLOWS FOR IDENTIFICATION OF INVASIVE IPMN Gastroenterology Dec 7 2020 [PMID: 33301777] (Human) Human
Wu H, Chen QY, Wang WZ et al. Compound sophorae decoction enhances intestinal barrier function of dextran sodium sulfate induced colitis via regulating notch signaling pathway in mice Biomed Pharmacother Nov 17 2020 [PMID: 33217689] (WB, Mouse) WB Mouse
Yu Y, Lu J, Oliphant K et al. Maternal administration of probiotics promotes gut development in mouse offsprings PLoS ONE Aug 7 2020 [PMID: 32764797] (Mouse) Mouse
Li B, Lee C et al. Inhibition of corticotropin-releasing hormone receptor 1 and activation of receptor 2 protect against colonic injury and promote epithelium repair. Sci Rep Nov 5 2017 [PMID: 31748698] (IHC, Mouse) IHC Mouse
Li B, Lee C, Filler T, Hock A Inhibition of corticotropin-releasing hormone receptor 1 and activation of receptor 2 protect against colonic injury and promote epithelium repair Sci Rep. 2017 May 10 [PMID: 28492284] (IHC, Mouse) IHC Mouse
Mishra J, Verma RK, Alpini G et al. Role of janus kinase 3 in mucosal differentiation and predisposition to colitis. J Biol Chem. 2013 Nov 1 [PMID: 24045942] (IHC-Fr, Mouse) IHC-Fr Mouse

Review for MUC2 Antibody (NB120-11197) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB120-11197:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
  3
reviewed by:
Verified Customer
IHC-P Other 03/11/2009
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedpig lung
SpeciesOther
Lot996/1
CommentsDid not work on pig as we were hoping.

Blocking

Blocking DetailsBlocking Buffer: background buster, Blocking Time: 30 minutes, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: several, Incubation Dilution Buffer: DAKO 1x buffer, Incubation Time: several, Incubation Temp: RT

Details

Detection NotesAntigen Retrieval: PK citrate buffer EDTA microwave steamer

Comments

CommentsDid not work on pig as we were hoping.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MUC2 Antibody (NB120-11197) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB120-11197AF350
Alexa Fluor 405 NB120-11197AF405
Alexa Fluor 488 NB120-11197AF488
Alexa Fluor 532 NB120-11197AF532
Alexa Fluor 594 NB120-11197AF594
Alexa Fluor 647 NB120-11197AF647
Alexa Fluor 700 NB120-11197AF700
Alexa Fluor 750 NB120-11197AF750
Allophycocyanin NB120-11197APC
Allophycocyanin/Cy7 NB120-11197APCCY7
Biotin NB120-11197B
DyLight 350 NB120-11197UV
DyLight 405 NB120-11197V
DyLight 488 NB120-11197G
DyLight 550 NB120-11197R
DyLight 594 NB120-11197DL594
DyLight 650 NB120-11197C
DyLight 680 NB120-11197FR
DyLight 755 NB120-11197IR
FITC NB120-11197F
HRP NB120-11197H
Janelia Fluor 549 NB120-11197JF549
Janelia Fluor 646 NB120-11197JF646
PE NB120-11197PE
PE/Atto594 NB120-11197PEATT594
PE/Cy5.5 NB120-11197PECY55
PE/Cy7 NB120-11197PECY7
PerCP NB120-11197PCP

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Recent Reviews

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4
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1
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Verified Customer
03/11/2009
Application: IHC-P
Species: Other

Bioinformatics

Gene Symbol MUC2
Entrez
Uniprot