Palladin Antibody (1E6) [DyLight 488]

• Reactivity: Hu, Mu, Rt, Ca, Ch
• Applications: WB, Flow, ICC/IF, IHC, IP, Mycoplasma
• Clone: 1E6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 488

Palladin Antibody (1E6) [DyLight 488] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Endogenous Palladin immunoprecipitated from chicken embryo fibroblasts.
• Localization: Cytoplasm, cytoskeleton. Note: Localizes to stress fibers and Z-disks. Cell junction, focal adhesion. Cell projection, ruffle. Cell projection, lamellipodium.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PALLD
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP1-25959. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 23451256).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Palladin Antibody (1E6) [DyLight 488]

• CGI151
• CGI-151
• FLJ22190
• FLJ38193
• KIAA0992FLJ39139
• MYN
• myoneurin
• Palladin
• palladin, cytoskeletal associated protein
• PALLD
• PNCA1
• Sarcoma antigen NY-SAR-77
• SIH002
• SIH002FLJ61376

Background

Palladin is a cytoskeletal protein required for organization of normal actin cytoskeleton. It plays roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. Palladin may function as a scaffolding molecule with the potential to impact both actin polymerization as well as the assembly of existing actin filaments into higher-order arrays. It binds to proteins that bind to either monomeric or filamentous actin. It localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Palladin's different isoforms may have functional differences. It is also involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Palladin Antibody (NBP1-25959G)(1)

Publication using NBP1-25959G

• van Steenbeek FG, Van den Bossche L, Grinwis GC et al. Aberrant Gene Expression in Dogs with Portosystemic Shunts. PLoS One 2013-01-01 [PMID: 23451256] (IHC-P, Canine)

Bioinformatics

• Gene Symbol: PALLD