XPF Antibody

Images

 
Western Blot: XPF Antibody [NB100-60679] - Detection of Human XPF/ERCC4 on HeLa whole cell lysate using NB100-60679.
Immunohistochemistry: XPF Antibody [NB100-60679] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- XPF/ERCC4 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: XPF Antibody [NB100-60679] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-XPF/ERCC4 used at a dilution of 1:500. Detection: DAB staining using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

XPF Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 855 and 905 of human xeroderma pigmentosum, complementation group F using the numbering given in entry AAB07689.1 (GeneID 2072).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ERCC4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • ELISA 1:100-1:2000
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. ELISA reactivity reported in scientific literature (PMID:20812782). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
XPF Lysate (NBL1-10322)
Publications
Read Publications using
NB100-60679 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for XPF Antibody

  • DNA excision repair protein ERCC-4
  • DNA repair protein complementing XP-F cells
  • EC 3.1
  • ERCC11
  • ERCC4
  • excision repair cross-complementing rodent repair deficiency, complementationgroup 4
  • xeroderma pigmentosum, complementation group F
  • XPF
  • XPFcomplementing defective, in Chinese hamster

Background

XPF/ERCC4 is suggested to play a role in the repair of DNA double-strand breaks (DSB), homologous recombination, and gene conversion via single-strand annealing (SSA). XPF/ERCC4 is an endonuclease that incises 5-prime DNA. Defects in XPF/ERCC4 cause xeroderma pigmentosum VI (XP6) an autosomal recessive disease characterized by hypersensitivity to sunlight and a predisposition to skin cancer as well as neurological abnormalities. Defects in XPF/ERCC4 are also responsible for XFE progeroid syndrome, a syndrome characterized by dwarfism, cachexia, and microcephaly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for XPF Antibody (NB100-60679)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ELISA, IP, WB.


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(1)
IP
(1)
WB
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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for XPF Antibody (NB100-60679)

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Blogs on XPF.

NER Antibodies in Cancer Research
We at Novus Biologicals have over 230 products in our antibody catalog devoted to nucleotide excision repair. NER is a multi-stage sequential process involving over 30 proteins, all of which have been widely studied. Being the primary method to repair...  Read full blog post.

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Bioinformatics

Gene Symbol ERCC4
Entrez
Uniprot