XRCC3 Antibody

Western Blot: XRCC3 Antibody [NB100-165] - (A) Human XRCC3 (hXRCC3) expression was down regulated by siRNA in human transofrmed fibroblasts. (B) XRCC3 was inactivated in human colon cancer cells by gene targeting [Yoshihara et all., 2004, EMBO J., 23 (3), 670-680]. hXRCC3 was detected by WB using XRCC3 antibody (NB100-165). The star indicates non-specific cross-reactivity of the antibody. Image provided by confirmed customer review.

Western Blot: XRCC3 Antibody [NB100-165] - Detection of XRCC3 (38 kDa) from Hela cell extract using NB100-165 (1:2500). Lanes 1 and 2 contain HTC116 and Hela whole cell extracts, respectively.

• Reactivity: Hu, Dr
• Applications: WB, IP, ICC/IF (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

XRCC3 Antibody Summary

• Immunogen: A synthetic partial peptide of human XRCC3. [UniProt# O43542]
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: XRCC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:5000
  • Immunoprecipitation
• Application Notes: This XRCC3 antibody is useful for Western blot, where a band can be seen at 38 kDa. No cross-reactivity was observed with XRCC2, RAD51, RAD51B, RAD51C, or RAD51D. The antibody is not applicable for Immunocytochemistry/Immunofluorescence. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Positive Control:

  XRCC3 Lysate (NBL1-17915)

Reactivity Notes

Human. Drosophila reactivity reported in scientific literature (PMID: 28743001).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for XRCC3 Antibody

• CMM6
• DNA repair protein XRCC3
• X-ray repair complementing defective repair in Chinese hamster cells 3
• X-ray repair cross-complementing protein 3

Background

XRCC3 (X-ray repair cross-complementing group 3) belongs to RecA family and participates in HRR/homologous recombination repair pathway which is an "error free" DNA repair mechanism that utilizes information encoded by homologous sequence to repair double-strand breaks (DSBs), chromosomal fragmentation, translocations and deletions. XRCC3 is required for Rad51 assembly/stabilization and in HRR pathway, XRCC3 protein interacts with Rad51 protein for contributing to chromosome integrity maintainance, thereby preventing mutations which can lead to carcinogenesis. In addition to repairing DSBs, XRCC3 also plays a role repairing wide range of DNA damage arising from carcinogens. Moreover, XRCC3 can control mtDNA copy number under conditions of oxidative stress in the presence of RAD51 as well as RAD51C. It forms a part of a complex consisting of RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3, and has been suggested to forms complex with FANCD2, BRCA2 and phosphorylated FANCG. XRCC3 also interacts with SWSAP1 and ZSWIM7 for executing homologous recombination repair and defective XRCC3 have been linked to susceptibility to breast cancer as well as cutaneous malignant melanoma type 6 (CMM6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XRCC3 Antibody (NB100-165)(13)

Publications using NB100-165

• Pae J, Cinalli RM, Marzio A et al. GCL and CUL3 Control the Switch between Cell Lineages by Mediating Localized Degradation of an RTK Dev. Cell 2017 Jul 24 [PMID: 28743001] (Drosophila)
• Parplys AC, Zhao W, Sharma N et al. NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability. Nucleic Acids Res. 2015 Aug 31 [PMID: 26323318] (WB, Human)
• Park JY, Singh TR, Nassar N et al. Breast cancer-associated missense mutants of the PALB2 WD40 domain, which directly binds RAD51C, RAD51 and BRCA2, disrupt DNA repair. Oncogene. 2013 Oct 21 [PMID: 24141787] (WB, Human)
• Girard PM, Graindorge D, Smirnova V et al. Oxidative Stress in Mammalian Cells Impinges on the Cysteines Redox State of Human XRCC3 Protein and on Its Cellular Localization. PLoS One. 2013 Oct 8 [PMID: 24116071] (WB, Hamster)
• Huang JW, Wang Y, Dhillon KK et al. Systematic Screen Identifies miRNAs That Target RAD51 and RAD51D to Enhance Chemosensitivity. Mol Cancer Res 2013 Dec [PMID: 24088786] (WB, Human)
• Al-Minawi AZ, Lee Y-F, Hakansson D et al. The ERCC1/XPF endonuclease is required for completion of homologous recombination at DNA replication forks stalled by inter-str cross-links. Nucleic Acids Res:gkp705. 2009 [PMID: 19713438]
• Katsura M, Tsuruga T, Date O et al. The ATR-Chk1 pathway plays a role in the generation of centrosome aberrations induced by Rad51C dysfunction. Nucleic Acids Res;37(12):3959-3968. 2009 [PMID: 19403737]
• Compton, SA et al. Xrcc3 and Nbs1 are required for the production of extrachromosomal telomeric circles in human alternative lengthening of telomere cells. Cancer Res;67(4):1513-9. 2007 Feb 15. [PMID: 17308089]
• Nagaraju, G et al. Differential regulation of short- and long-tract gene conversion between sister chromatids by Rad51C. Mol Cell Biol;26(21):8075-86. 2006 Nov. [PMID: 16954385] (WB, Human)
• Szuts, D et al. Role for RAD18 in homologous recombination in DT40 cells. Mol Cell Biol;26(21):8032-41. 2006 Nov. [PMID: 16923963] (WB, Human)
• Yoshihara, T et al. XRCC3 deficiency results in a defect in recombination and increased endoreduplication in human cells. EMBO J;23(3):670-80. 2004 Feb 11. [PMID: 14749735] (IP, Human)
• Girard PM, Francesconi S, Pozzebon M et al. UVA-induced damage to DNA and proteins: direct versus indirect photochemical processes. J. Phys.: Conf. Ser. 2011
• Nicolay NH, Carter R, Hatch SB et al. Homologous recombination mediates S-phase-dependent radioresistance in cells deficient in DNA polymerase eta Carcinogenesis 2012 Sep 3 [PMID: 22822095] (WB, Human)

Review for XRCC3 Antibody (NB100-165) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 01/08/2014

Summary

• Application: Western Blot
• Sample Tested: Human transformed fibroblasts whole cell extract
• Species: Human

Blocking

• Blocking Details: Nitrocellulose membranes were blocked 1h at room temperature in 5% nonfat dry milk diluted in TBS-0.05% tween (TBS-T)

Primary Anitbody

• Dilution Ratio: 1/10000 in 5%BSA in TBS-T over-night 4°C or 1h at room temperature

Secondary Antibody

• Secondary Description: Goat anti-rabbit IgG HRP
• Secondary Manufacturer Cat#: Jackson ImmunoResearch #111-035-003
• Secondary Concentration: 1/10000 to 1/20000 in 5% nonfat dry milk in TBS-T

Details

• Detection Notes: autoradiography from few seconds to 1 min depending on the ECL detection reagents (normal or plus)

Bioinformatics

• Gene Symbol: XRCC3