XRCC3 Antibody - BSA Free

Images

 
Genetic Strategies: Western Blot: XRCC3 Antibody [NB100-165] - (A) Human XRCC3 (hXRCC3) expression was down regulated by siRNA in human transofrmed fibroblasts. (B) XRCC3 was inactivated in human colon cancer ...read more
Western Blot: XRCC3 Antibody [NB100-165] - Detection of XRCC3 (38 kDa) from HeLa cell extract using NB100-165 (1:2500). Lanes 1 and 2 contain HTC116 and Hela whole cell extracts, respectively.

Product Details

Summary
Reactivity Hu, Dr, HaSpecies Glossary
Applications WB, IP, ICC/IF (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

XRCC3 Antibody - BSA Free Summary

Immunogen
A synthetic partial peptide of human XRCC3. [UniProt# O43542]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XRCC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation
  • Western Blot 1:5000
Application Notes
This XRCC3 antibody is useful for Western blot, where a band can be seen at 38 kDa. No cross-reactivity was observed with XRCC2, RAD51, RAD51B, RAD51C, or RAD51D. The antibody is not applicable for Immunocytochemistry/Immunofluorescence.
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
XRCC3 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-165 in the following applications:

Publications
Read Publications using
NB100-165 in the following applications:

  • IP
    2 publications
  • WB
    9 publications

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 28743001). Hamster reactivity reported in scientific literature (PMID: 24116071).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for XRCC3 Antibody - BSA Free

  • CMM6
  • DNA repair protein XRCC3
  • X-ray repair complementing defective repair in Chinese hamster cells 3
  • X-ray repair cross-complementing protein 3

Background

XRCC3 (X-ray repair cross-complementing group 3) belongs to RecA family and participates in HRR/homologous recombination repair pathway which is an "error free" DNA repair mechanism that utilizes information encoded by homologous sequence to repair double-strand breaks (DSBs), chromosomal fragmentation, translocations and deletions. XRCC3 is required for Rad51 assembly/stabilization and in HRR pathway, XRCC3 protein interacts with Rad51 protein for contributing to chromosome integrity maintainance, thereby preventing mutations which can lead to carcinogenesis. In addition to repairing DSBs, XRCC3 also plays a role repairing wide range of DNA damage arising from carcinogens. Moreover, XRCC3 can control mtDNA copy number under conditions of oxidative stress in the presence of RAD51 as well as RAD51C. It forms a part of a complex consisting of RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3, and has been suggested to forms complex with FANCD2, BRCA2 and phosphorylated FANCG. XRCC3 also interacts with SWSAP1 and ZSWIM7 for executing homologous recombination repair and defective XRCC3 have been linked to susceptibility to breast cancer as well as cutaneous malignant melanoma type 6 (CMM6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XRCC3 Antibody (NB100-165)(15)

We have publications tested in 3 confirmed species: Human, Drosophila, Hamster.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(2)
WB
(9)
All Applications
Filter By Species
Human
(8)
Drosophila
(1)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB100-165 Applications Species
Longo MA, Roy S, Chen Y et al. RAD51C-XRCC3 structure and cancer patient mutations define DNA replication roles Nature communications 2023-07-24 [PMID: 37488098] (WB, IP) WB, IP
Shafi AA, McNair CM, McCann JJ, et al. The circadian cryptochrome, CRY1, is a pro-tumorigenic factor that rhythmically modulates DNA repair Nature communications 2021-01-15 [PMID: 33452241] (WB, Human) WB Human
Pae J, Cinalli RM, Marzio A et al. GCL and CUL3 Control the Switch between Cell Lineages by Mediating Localized Degradation of an RTK Dev. Cell 2017-07-24 [PMID: 28743001] (Drosophila) Drosophila
Parplys AC, Zhao W, Sharma N et al. NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability. Nucleic Acids Res. 2015-08-31 [PMID: 26323318] (WB, Human) WB Human
Park JY, Singh TR, Nassar N et al. Breast cancer-associated missense mutants of the PALB2 WD40 domain, which directly binds RAD51C, RAD51 and BRCA2, disrupt DNA repair. Oncogene. 2013-10-21 [PMID: 24141787] (WB, Human) WB Human
Girard PM, Graindorge D, Smirnova V et al. Oxidative Stress in Mammalian Cells Impinges on the Cysteines Redox State of Human XRCC3 Protein and on Its Cellular Localization. PLoS One. 2013-10-08 [PMID: 24116071] (WB, Hamster) WB Hamster
Huang JW, Wang Y, Dhillon KK et al. Systematic Screen Identifies miRNAs That Target RAD51 and RAD51D to Enhance Chemosensitivity. Mol Cancer Res 2013-12-01 [PMID: 24088786] (WB, Human) WB Human
Nicolay NH, Carter R, Hatch SB et al. Homologous recombination mediates S-phase-dependent radioresistance in cells deficient in DNA polymerase eta Carcinogenesis 2012-09-03 [PMID: 22822095] (WB, Human) WB Human
Al-Minawi AZ, Lee Y-F, Hakansson D et al. The ERCC1/XPF endonuclease is required for completion of homologous recombination at DNA replication forks stalled by inter-str cross-links. Nucleic Acids Res:gkp705. 2009-01-01 [PMID: 19713438]
Katsura M, Tsuruga T, Date O et al. The ATR-Chk1 pathway plays a role in the generation of centrosome aberrations induced by Rad51C dysfunction. Nucleic Acids Res;37(12):3959-3968. 2009-01-01 [PMID: 19403737]
Show All 15 Publications.

Review for XRCC3 Antibody (NB100-165) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-165:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot XRCC3 NB100-165
Enlarge
5
reviewed by:
Verified Customer
WB Human 01/08/2014
View

Summary

ApplicationWestern Blot
Sample TestedHuman transformed fibroblasts whole cell extract
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for XRCC3 Antibody (NB100-165) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
01/08/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol XRCC3