XRCC3 Antibody

Images

 
Western Blot: XRCC3 Antibody [NB100-165] - (A) Human XRCC3 (hXRCC3) expression was down regulated by siRNA in human transofrmed fibroblasts. (B) XRCC3 was inactivated in human colon cancer cells by gene targeting ...read more
Western Blot: XRCC3 Antibody [NB100-165] - Detection of XRCC3 (38 kDa) from Hela cell extract using NB100-165 (1:2500). Lanes 1 and 2 contain HTC116 and Hela whole cell extracts, respectively.

Product Details

Summary
Reactivity Hu, DrSpecies Glossary
Applications WB, IP, ICC/IF (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
     

Genetic Strategies

   

XRCC3 Antibody Summary

Immunogen
A synthetic partial peptide of human XRCC3. [UniProt# O43542]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XRCC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Immunoprecipitation
Application Notes
This XRCC3 antibody is useful for Western blot, where a band can be seen at 38 kDa. No cross-reactivity was observed with XRCC2, RAD51, RAD51B, RAD51C, or RAD51D. The antibody is not applicable for Immunocytochemistry/Immunofluorescence. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
XRCC3 Lysate (NBL1-17915)
Reviewed Applications
Read 1 Review rated 5
using
NB100-165 in the following applications:

Publications
Read Publications using
NB100-165 in the following applications:

  • IP
    1 publication
  • WB
    7 publications

Reactivity Notes

Human. Drosophila reactivity reported in scientific literature (PMID: 28743001).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for XRCC3 Antibody

  • CMM6
  • DNA repair protein XRCC3
  • X-ray repair complementing defective repair in Chinese hamster cells 3
  • X-ray repair cross-complementing protein 3

Background

XRCC3 (X-ray repair cross-complementing group 3) belongs to RecA family and participates in HRR/homologous recombination repair pathway which is an "error free" DNA repair mechanism that utilizes information encoded by homologous sequence to repair double-strand breaks (DSBs), chromosomal fragmentation, translocations and deletions. XRCC3 is required for Rad51 assembly/stabilization and in HRR pathway, XRCC3 protein interacts with Rad51 protein for contributing to chromosome integrity maintainance, thereby preventing mutations which can lead to carcinogenesis. In addition to repairing DSBs, XRCC3 also plays a role repairing wide range of DNA damage arising from carcinogens. Moreover, XRCC3 can control mtDNA copy number under conditions of oxidative stress in the presence of RAD51 as well as RAD51C. It forms a part of a complex consisting of RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3, and has been suggested to forms complex with FANCD2, BRCA2 and phosphorylated FANCG. XRCC3 also interacts with SWSAP1 and ZSWIM7 for executing homologous recombination repair and defective XRCC3 have been linked to susceptibility to breast cancer as well as cutaneous malignant melanoma type 6 (CMM6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for XRCC3 Antibody (NB100-165)(13)

We have publications tested in 3 confirmed species: Human, Drosophila, Hamster.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(1)
WB
(7)
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Filter By Species
Human
(7)
Drosophila
(1)
Hamster
(1)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB100-165 Applications Species
Pae J, Cinalli RM, Marzio A et al. GCL and CUL3 Control the Switch between Cell Lineages by Mediating Localized Degradation of an RTK Dev. Cell 2017 Jul 24 [PMID: 28743001] (Drosophila) Drosophila
Parplys AC, Zhao W, Sharma N et al. NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability. Nucleic Acids Res. 2015 Aug 31 [PMID: 26323318] (WB, Human) WB Human
Park JY, Singh TR, Nassar N et al. Breast cancer-associated missense mutants of the PALB2 WD40 domain, which directly binds RAD51C, RAD51 and BRCA2, disrupt DNA repair. Oncogene. 2013 Oct 21 [PMID: 24141787] (WB, Human) WB Human
Girard PM, Graindorge D, Smirnova V et al. Oxidative Stress in Mammalian Cells Impinges on the Cysteines Redox State of Human XRCC3 Protein and on Its Cellular Localization. PLoS One. 2013 Oct 8 [PMID: 24116071] (WB, Hamster) WB Hamster
Huang JW, Wang Y, Dhillon KK et al. Systematic Screen Identifies miRNAs That Target RAD51 and RAD51D to Enhance Chemosensitivity. Mol Cancer Res 2013 Dec [PMID: 24088786] (WB, Human) WB Human
Nicolay NH, Carter R, Hatch SB et al. Homologous recombination mediates S-phase-dependent radioresistance in cells deficient in DNA polymerase eta Carcinogenesis 2012 Sep 3 [PMID: 22822095] (WB, Human) WB Human
Al-Minawi AZ, Lee Y-F, Hakansson D et al. The ERCC1/XPF endonuclease is required for completion of homologous recombination at DNA replication forks stalled by inter-str cross-links. Nucleic Acids Res:gkp705. 2009 [PMID: 19713438]
Katsura M, Tsuruga T, Date O et al. The ATR-Chk1 pathway plays a role in the generation of centrosome aberrations induced by Rad51C dysfunction. Nucleic Acids Res;37(12):3959-3968. 2009 [PMID: 19403737]
Compton, SA et al. Xrcc3 and Nbs1 are required for the production of extrachromosomal telomeric circles in human alternative lengthening of telomere cells. Cancer Res;67(4):1513-9. 2007 Feb 15. [PMID: 17308089]
Nagaraju, G et al. Differential regulation of short- and long-tract gene conversion between sister chromatids by Rad51C. Mol Cell Biol;26(21):8075-86. 2006 Nov. [PMID: 16954385] (WB, Human) WB Human
Show All 13 Publications.

Review for XRCC3 Antibody (NB100-165) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-165:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot XRCC3 NB100-165
Enlarge
reviewed by:
Anonymous
WB Human 01/08/2014
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Summary

ApplicationWestern Blot
Sample TestedHuman transformed fibroblasts whole cell extract
SpeciesHuman

Blocking

Blocking DetailsNitrocellulose membranes were blocked 1h at room temperature in 5% nonfat dry milk diluted in TBS-0.05% tween (TBS-T)

Primary Anitbody

Dilution Ratio1/10000 in 5%BSA in TBS-T over-night 4°C or 1h at room temperature

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG HRP
Secondary Manufacturer Cat#Jackson ImmunoResearch #111-035-003
Secondary Concentration1/10000 to 1/20000 in 5% nonfat dry milk in TBS-T

Details

Detection Notesautoradiography from few seconds to 1 min depending on the ECL detection reagents (normal or plus)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for XRCC3 Antibody (NB100-165) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-165AF405
Alexa Fluor 488 Labeled NB100-165AF488
Alexa Fluor 647 Labeled NB100-165AF647
Alexa Fluor 700 Labeled NB100-165AF700
Biotin Labeled NB100-165B
DyLight 405 Labeled NB100-165V
DyLight 488 Labeled NB100-165G
DyLight 550 Labeled NB100-165R
DyLight 650 Labeled NB100-165C
FITC Labeled NB100-165F
HRP Labeled NB100-165H

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
01/08/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol XRCC3