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XPB Antibody

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Western Blot: XPB Antibody [NB100-61060] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiERCC3 antibody used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: XPB Antibody [NB100-61060] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiERCC3 antibody ...read more
Independent Antibodies: Immunoprecipitation: XPB Antibody [NB100-61060] - Detection of human ERCC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, ChIP, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

        

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XPB Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 732 and 782 of human excision repair cross-complementing rodent repair deficiency, complementation group 3 using the numbering given in entry NP_000113.1 (GeneID 2071).
Predicted Species
Rat (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ERCC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID 24609361)
Control
XPB Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-61060 in the following applications:
Publications
Read Publication using
NB100-61060 in the following applications:

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for XPB Antibody

  • Basic transcription factor 2 89 kDa subunit
  • BTF2 p89
  • BTF2
  • DNA excision repair protein ERCC-3
  • DNA repair protein complementing XP-B cells
  • EC 3.6.1
  • EC 3.6.4.12
  • ERCC3
  • excision repair cross-complementing rodent repair deficiency, complementationgroup 3 (xeroderma pigmentosum group B complementing)
  • GTF2H
  • RAD25
  • TFIIH basal transcription factor complex 89 kDa subunit
  • TFIIH basal transcription factor complex helicase XPB subunit
  • TFIIH p89
  • TFIIH
  • Xeroderma pigmentosum group B-complementing protein
  • xeroderma pigmentosum, complementation group B
  • XPB
  • XPBC
  • XPBTFIIH 89 kDa subunit

Background

ERCC3 is a component of the core TFIIH basal transcription factor and functions as an ATP-dependent 3'-5' DNA helicase. ERCC3 is involved in nucleotide excision repair and is the cause of xeroderma pigmentosum complementation group B (XPB), also known as xeroderma pigmentosum II (XP2). Additionally, it has been found to be the cuase of Cockayne syndrome, and trichothiodystrophy (TTD). XPB is an autosomal recessive disease characterized by skin photosensitivity and a predisposition to skin cancer, and neurological abnormalities. Cockayne syndrome and TTD are similarly characterized by photosensitivity and neural abnormalities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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LXR beta/NR1H2
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Western Blot: LXR beta/NR1H2 Antibody [NB100-74457] - 25 ug of rat liver (Lane 1), mouse liver (Lane 2) and Hela (Lane 3) cell lysates.
Immunocytochemistry/Immunofluorescence: LXR beta/NR1H2 Antibody [NB100-74457] - Analysis of LXR beta (green) showing positive staining in the nuclear envelope of C2C12 cells (right) compared with a negative control in the absence of primary antibody (left).
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
Cdk7 Antibody
(JJ203-01)
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Immunocytochemistry/Immunofluorescence: Cdk7 Antibody (JJ203-01) [NBP2-67850] - Staining Cdk7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Cdk7 Antibody (JJ203-01) [NBP2-67850] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cdk7 antibody. Counter stained with hematoxylin.
Flow Cytometry: Cdk7 Antibody (JJ203-01) [NBP2-67850] - Analysis of A431 cells with Cdk7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Cyclin H Antibody
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Western Blot: Cyclin H Antibody (SN20-48) [NBP2-67719] - Analysis of Cyclin H on K562 cells lysates using anti-Cyclin H antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: Cyclin H Antibody (SN20-48) [NBP2-67719] - Staining Cyclin H in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Cyclin H Antibody (SN20-48) [NBP2-67719] - Analysis of paraffin-embedded mouse testis tissue using anti-Cyclin H antibody. Counter stained with hematoxylin.
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
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Western Blot: XPG Antibody [NB100-74611] - Detection of Human ERCC5/XPG on HeLa whole cell lysate using Nb100-74611. ERCC5/XPG was also immunoprecipitated by rabbit anti-ERCC5/XPG antibody NB100-74612.
Immunohistochemistry: XPG Antibody [NB100-74611] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ERCC5/XPG used at a dilution of 1:200 (1ug/ml). Detection: DAB
(2 Publications)
Species: Hu
Applications: WB, IHC, IHC-P, IP
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NB100-93321
Western Blot: XPA Antibody [NB100-93321] - Detection of human XPA by western blot. Samples: Whole cell lysate (50 µg) from HeLa, K-562, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XPA antibody NB100-93321 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry: XPA Antibody [NB100-93321] - Detection of human XPA by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- XPA (NB100-93321). Detection: DAB.
Western Blot: XPA Antibody [NB100-93321] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-93321 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate
Species: Hu
Applications: WB, IHC, IHC-P, IP
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NB100-477
Western Blot: XPC Antibody (3.26) [NB100-477] - Whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with XPC antibody [3.26] diluted at 1:500.
Immunocytochemistry/Immunofluorescence: XPC Antibody (3.26) [NB100-477] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: XPC protein stained by XPC antibody [3.26] diluted at 1:100.Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.
Western Blot: XPC Antibody (3.26) [NB100-477] - HeLa whole cell lysate detected using rabbit polyclonal.
(1 Review)
(17 Publications)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
TATA binding
protein TBP Antibody ...
NBP2-59208
Western Blot: TATA binding protein TBP Antibody (C.15200002) [NBP2-59208] - Whole cell extracts (40 ug) from HeLa cells transfected with TBP siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against TBP diluted 1:500 in TBSTween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Chromatin Immunoprecipitation Sequencing: TATA binding protein TBP Antibody (C.15200002) [NBP2-59208] - IP'd DNA was analyzed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets (A). The IP'd DNA was analyzed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.
Chromatin Immunoprecipitation: TATA binding protein TBP Antibody (C.15200002) [NBP2-59208] - ChIP results obtained with the antibody against TBP. ChIP assays were performed using U2OS cells, the antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes, a region 0.5 and 1 kb upstream of the GAPDH promoter, respectively, and for exon 2 of the myoglobin gene as a negative control. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.
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Applications: WB, ChIP, ChIP, CHIP-SEQ, KD
CSB Knockout HeLa
Cell Lysate
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CSB Knockout HeLa Cell Lysate
Species: Hu
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(1F12-1B5)
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Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 Analysis of GTF2H1 expression in Jurkat.
Immunocytochemistry/Immunofluorescence: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue.Antibody concentration 5 ug/ml.
(2 Publications)
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, RNAi
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Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Formalin fixed paraffin embedded human tonsil stained with ERCC1 antibody.
Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Analysis of ERCC1 in human non-small cell lung cancer. Image courtesy of product review submitted by Alex Soltermann.
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Species: Hu
DDR1 Antibody
[Unconjugated]
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(1 Review)
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RAP30 Antibody
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Western Blot: RAP30 Antibody (4B10) [NBP2-01265] Analysis of extracts (35ug) from 9 different cell lines by using anti-RAP30 monoclonal antibody.
Immunohistochemistry-Paraffin: RAP30 Antibody (4B10) [NBP2-01265] - Staining of paraffin-embedded Human endometrium tissue using anti-RAP30 mouse monoclonal antibody.
Flow Cytometry: RAP30 Antibody (4B10) [NBP2-01265] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RAP30 antibody, and then analyzed by flow cytometry.
Species: Hu, Ca, Pm
Applications: WB, Flow, IHC, IHC-P
RAP74 Antibody
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Western Blot: RAP74 Antibody [NBP1-89922] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-459
Immunocytochemistry/Immunofluorescence: RAP74 Antibody [NBP1-89922] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & microtubules.
Immunohistochemistry-Paraffin: RAP74 Antibody [NBP1-89922] - Staining of human rectum shows strong nuclear positivity in glandular cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
p53 Antibody (PAb
240)
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Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
(3 Reviews)
(23 Publications)
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
XPF Antibody
(OTI4E11)
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Western Blot: XPF Antibody (4E11) [NBP2-01020] Analysis of extracts (35ug) from 9 different cell lines by using anti-XPF monoclonal antibody.
Immunocytochemistry/Immunofluorescence: XPF Antibody (4E11) [NBP2-01020] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY XPF.
Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Human tonsil using anti-XPF mouse monoclonal antibody.
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
p62/SQSTM1
Antibody (2C11)
H00008878-M01
Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of recombinant p62 protein (immunogen, 74.51 KDa) using SQSTM1/p62 antibody (clone 2C11).
Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of p62 in p62 WT and KO mouse hepatocytes and HepG2 cell lysate using anti-p62/SQSTM1 antibody clone 2C11. Sample lane1: p62 +/- mouse hepatocytes, lane 2: p62 -/- mouse hepatocytes, lane3: Wt mouse hepatocytes, lane 4: HepG2 cells. This image is from a verified customer product review.
Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - ICC-IF analysis of SQSTM1 protein in HeLa cell using SQSTM1/p62 antibody (clone 2C11) at 10 ug/ml concentration.
(7 Reviews)
(214 Publications)
Species: Hu, Mu, Rt, Rb
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NUP62 Antibody
NBP1-31381
Western Blot: NUP62 Antibody [NBP1-31381] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and blotted with Nucleoporin p62 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Nucleoporin p62 Antibody [NBP1-31381] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleoporin p62 protein stained by Nucleoporin p62 antibody diluted at 1:750. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: Nucleoporin p62 Antibody [NBP1-31381] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
dynactin 4
Antibody (3G9D7)
NBP2-37399
Western Blot: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Western blot analysis using DCTN4 mouse mAb against Raw264.7 (1) and NIH3T3 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Immunofluorescence analysis of HepG2 cells using DCTN4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using DCTN4 mouse mAb with DAB staining.
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready

Publications for XPB Antibody (NB100-61060)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ChIP.


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Review for XPB Antibody (NB100-61060) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-61060:
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Images Ratings Applications Species Date Details
 
reviewed by:
Christophe Martella
 ChIP Human 04/23/2019
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Summary

ApplicationChromatin Immunoprecipitation
Sample TestedCD8 T lymphocytes,human lymphocytes, whole cell lysate
SpeciesHuman
LotA1

Comments

CommentschIP on T lymphocyte

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FAQs for XPB Antibody (NB100-61060) (0)

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Control Lysate(s)

XPB Overexpression Lysate

Secondary Antibodies

 

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Recent Reviews

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 Christophe Martella
04/23/2019
Application: ChIP
Species: Human
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Bioinformatics

Gene Symbol ERCC3
Entrez
Uniprot