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XPB Antibody

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Western Blot: XPB Antibody [NB100-61060] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiERCC3 antibody used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: XPB Antibody [NB100-61060] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiERCC3 antibody ...read more
Independent Antibodies: Immunoprecipitation: XPB Antibody [NB100-61060] - Detection of human ERCC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from ...read more

Product Details

Summary
Reactivity Hu, Mu, V-Vi, Rt, Bv, Pm-OrSpecies Glossary
Applications WB, ChIP, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

        

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XPB Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 732 and 782 of human excision repair cross-complementing rodent repair deficiency, complementation group 3 using the numbering given in entry NP_000113.1 (GeneID 2071).
Predicted Species
Rat (100%), Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ERCC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:32434920).
Control
XPB Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-61060 in the following applications:
Publications
Read Publications using
NB100-61060 in the following applications:

  • ChIP
    2 publications

Reactivity Notes

Use in Epstein-Barr virus reported in scientific publication (PMID: 32434920).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for XPB Antibody

  • Basic transcription factor 2 89 kDa subunit
  • BTF2 p89
  • BTF2
  • DNA excision repair protein ERCC-3
  • DNA repair protein complementing XP-B cells
  • EC 3.6.1
  • EC 3.6.4.12
  • ERCC3
  • excision repair cross-complementing rodent repair deficiency, complementationgroup 3 (xeroderma pigmentosum group B complementing)
  • GTF2H
  • RAD25
  • TFIIH basal transcription factor complex 89 kDa subunit
  • TFIIH basal transcription factor complex helicase XPB subunit
  • TFIIH p89
  • TFIIH
  • Xeroderma pigmentosum group B-complementing protein
  • xeroderma pigmentosum, complementation group B
  • XPB
  • XPBC
  • XPBTFIIH 89 kDa subunit

Background

ERCC3 is a component of the core TFIIH basal transcription factor and functions as an ATP-dependent 3'-5' DNA helicase. ERCC3 is involved in nucleotide excision repair and is the cause of xeroderma pigmentosum complementation group B (XPB), also known as xeroderma pigmentosum II (XP2). Additionally, it has been found to be the cuase of Cockayne syndrome, and trichothiodystrophy (TTD). XPB is an autosomal recessive disease characterized by skin photosensitivity and a predisposition to skin cancer, and neurological abnormalities. Cockayne syndrome and TTD are similarly characterized by photosensitivity and neural abnormalities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunoprecipitation: Cdk7 Antibody [NB100-581] - Detection of human CDK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDK7 antibody NB100-581 (lot NB100-581-2) used for IP at 6 ug per reaction. CDK7 was also immunoprecipitated by a previous lot of this antibody NB100-581-1 For blotting immunoprecipitated CDK7, NB100-581 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: Cdk7 Antibody [NB100-581] - Analysis using the Biotin conjugate of NB100-581. Detection ofHeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 minutes.
Species: Hu, Mu, Rt
Applications: ICC, IHC, IP, WB
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Flow Cytometry: Cyclin H Antibody (SN20-48) [NBP2-67719] - Analysis of Hela cells with Cyclin H antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: Cyclin H Antibody (SN20-48) [NBP2-67719] - Staining Cyclin H in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
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Immunoprecipitation: XPG Antibody [NB100-74611] - Detection of human ERCC5/XPG by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ERCC5/XPG antibody NB100-74611 used for IP at 6 ug per reaction. For blotting immunoprecipitated ERCC5/XPG, NB100-74611 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: XPG Antibody [NB100-74611] - Detection of human ERCC5/XPG by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ERCC5/XPG antibody NB100-74611 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: XPG Antibody [NB100-74611] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ERCC5/XPG used at a dilution of 1:200 (1ug/ml). Detection: DAB
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPA Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3416) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPA at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 5.
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Immunocytochemistry/Immunofluorescence: XPC Antibody (3.26) [NB100-477] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: XPC protein stained by XPC antibody [3.26] diluted at 1:100.Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.
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Immunocytochemistry/Immunofluorescence: XPC Antibody (3.26) [NB100-477] - Left: HeLa cells stained with XPC (3.26) monoclonal antibody (NB100-477). Right: DAPI Staining Photo is kindly provided by Dr. S. Liu at U of Arizona.
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Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody (M01), clone 1F12-1B5.Lane 1: GTF2H1 transfected lysate(62 KDa).Lane 2: Non-transfected lysate.
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Immunohistochemistry-Paraffin: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue.Antibody concentration 5 ug/ml.
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(1 Review)
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Flow Cytometry: RAP30 Antibody (4B10) [NBP2-01265] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RAP30 antibody, and then analyzed by flow cytometry.
Western Blot: RAP30 Antibody (4B10) [NBP2-01265] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAP30 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAP30.
Immunohistochemistry-Paraffin: RAP30 Antibody (4B10) [NBP2-01265] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RAP30 mouse monoclonal antibody.
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Applications: Flow, IHC, IHC-P, WB
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Immunocytochemistry/Immunofluorescence: RAP74 Antibody [NBP1-89922] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & microtubules.
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Applications: ICC/IF, IHC, IHC-P, WB
p53 Antibody (PAb
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Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.
(3 Reviews)
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Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
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Flow Cytometry: XPF Antibody (4E11) [NBP2-01020] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-XPF antibody, and then analyzed by flow cytometry.
Western Blot: XPF Antibody (4E11) [NBP2-01020] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XPF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XPF.
Immunohistochemistry-Paraffin: XPF Antibody (4E11) [NBP2-01020] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-XPF mouse monoclonal antibody.
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
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Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Analysis of p62 in SH-SY5Y cells using anti-p62/SQSTM1 antibody. Red - p62 puncta; Blue - nuclear DAPI; Green - Cytoskeleton. Image from verified customer review.
Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Confirmation of loss of hepatic ATG7 protein and a phenotype of hepatic autophagy inactivation. Fourth ug of liver protein were loaded on 4-20% gradient Tris-Glycine gels and subsequently immunoblotted with antibodies against ATG7, p62 and LC3. Blots were visualized with G:BOX Chemi XRQ (SynGene). Representative immunoblots are shown for WT and KO mice. Full-length immunoblots are presented in Supplementary Figure S1. ATG7: autophagy-related protein 7; p62: sequestosome 1; LC3: microtubule-associated protein 1 light chain 3; WT: Mx1-Cre-Atg7F/F mouse; KO: Mx1-Cre+Atg7F/F mouse. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-017-14405-w), licensed under a CC-BY licence.
Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - Disruption of the VANGL2-p62/SQSTM1 interaction in breast cancer cells. Soft agar colony formation of T47D cells overexpressing GFP, GFP-VANGL2 and GFP-p62/SQSTM1 (right). Protein expression was revealed with anti-p62/SQSTM1, anti-VANGL2 and anti-GFP antibodies by western blot analysis (right). In anti-GFP blot, the arrowhead indicates position of co-migrating GFP-VANGL2 and GFP-p62/SQSTM1 and the asterisk pinpoints GFP alone. Error bars represent mean+/-s.d. (n=3). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/ncomms10318), licensed under a CC-BY licence.
(8 Reviews)
(253 Publications)
Species: Bv, Ha, Hu, Mu, Rb, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NUP62 Antibody
NBP1-31381
Western Blot: NUP62 Antibody [NBP1-31381] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and blotted with Nucleoporin p62 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Nucleoporin p62 Antibody [NBP1-31381] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleoporin p62 protein stained by Nucleoporin p62 antibody diluted at 1:750. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Western Blot: Nucleoporin p62 Antibody [NBP1-31381] - PC-12 whole cell lysate (30 ug) stained wtih Nucleoporin p62 Antibody diluted at 1:5000.
(3 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
dynactin 4
Antibody (3G9D7)
NBP2-37399
ELISA: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western Blot: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Western blot analysis using DCTN4 mAb against human DCTN4 recombinant protein. (Expected MW is 53.2 kDa)
Western Blot: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Western blot analysis using DCTN4 mAb against HEK293 (1) and DCTN4 (AA: ***)-hIgGFc transfected HEK293 (2) cell lysate.
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB

Publications for XPB Antibody (NB100-61060)(2)

We have publications tested in 2 confirmed species: Human, Virus.

We have publications tested in 1 application: ChIP.


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(2)
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Review for XPB Antibody (NB100-61060) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-61060:
Filter by Applications
ChIP
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Christophe Martella
 ChIP Human 04/23/2019
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Summary

ApplicationChromatin Immunoprecipitation
Sample TestedCD8 T lymphocytes,human lymphocytes, whole cell lysate
SpeciesHuman
LotA1

Comments

CommentschIP on T lymphocyte

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FAQs for XPB Antibody (NB100-61060) (0)

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Control Lysate(s)

XPB Overexpression Lysate

Secondary Antibodies

 

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 Christophe Martella
04/23/2019
Application: ChIP
Species: Human
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Bioinformatics

Gene Symbol ERCC3
Entrez
Uniprot