XPC Antibody (3.26)

Western Blot: XPC Antibody (3.26) [NB100-477] - DNA repair protein expression in tumors. A. Western blot determination of p53, XPC, XPA, ERCC1 and MSH2 in tumors. GAPDH is loading control. Image collected and cropped by CiteAb from the following publication (//ovarianresearch.biomedcentral.com/articles/10.1186/1757-2215-4-9) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: XPC Antibody (3.26) [NB100-477] - Left: HeLa cells stained with XPC (3.26) monoclonal antibody (NB100-477). Right: DAPI Staining Photo is kindly provided by Dr. S. Liu at U of Arizona.

Western Blot: XPC Antibody (3.26) [NB100-477] - HeLa whole cell lysate detected using rabbit polyclonal .

Western Blot: XPC Antibody (3.26) [NB100-477] - Whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with XPC antibody [3.26] diluted at 1:500.

Immunocytochemistry/Immunofluorescence: XPC Antibody (3.26) [NB100-477] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: XPC protein stained by XPC antibody [3.26] diluted at 1:100.Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clone: 3.26
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

XPC Antibody (3.26) Summary

• Immunogen: Recombinant XPC purified from E. coli
• Localization: Nuclear
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: XPC
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000
• Application Notes: IHC-P (Paraffin sections) Assay dependent
• Theoretical MW: 123 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Raji Whole Cell Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 17229834). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for XPC Antibody (3.26)

• complementation group C antibody
• p125
• RAD4
• Xeroderma pigmentosum group C-complementing protein
• xeroderma pigmentosum, complementation group C
• XP3
• XPCCDNA repair protein complementing XP-C cells

Background

Human XPC (Xeroderma pigmentosum group C) is a member of a family of proteins that has been shown to be involved in the repair of DNA via the nucleotide excision repair (NER) pathway. Specifically, XPC is believed to be a part of a heteromeric protein complex that is involved in the recognition of the DNA lesions during global genomic repair but not transcription-coupled repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XPC Antibody (NB100-477)(17)

Publications using NB100-477

• Fast OG, Gentry B, Strouth L et al. Polynuclear ruthenium organometallic compounds induce DNA damage in human cells identified by the nucleotide excision repair factor XPC Biosci. Rep. 2019-06-21 [PMID: 31227614] (IF, Human)
• Beresova L, Vesela E, Chamrad I et al. Role of DNA Repair Factor Xeroderma Pigmentosum Protein Group C in Response to Replication Stress As Revealed by DNA Fragile Site Affinity Chromatography and Quantitative Proteomics. J. Proteome Res. 2016-12-02 [PMID: 27794614]
• Mohni KN, Kavanaugh GM, Cortez D. ATR pathway inhibition is synthetically lethal in cancer cells with ERCC1 deficiency. Cancer Res. 2014-03-24 [PMID: 24662920] (WB, Human)
• Maria Berra C, Santos de Oliveira C, Carriao Machado Garcia C et al. Nucleotide excision repair activity on DNA damage induced by photoactivated methylene blue. Free Radic Biol Med 2013-04-05 [PMID: 23567189] (WB, Human)
• Kothandapani A, Gopalakrishnan K, Kahali B et al. Downregulation Of Swi/Snf Chromatin Remodeling Factor Subunits Modulate Cisplatin Cytotoxicity Exp Cell Res 2012-06-18 [PMID: 22721696] (ICC/IF, Human)
• Muenyi CS, States VA, Masters JH et al. Sodium arsenite and hyperthermia modulate cisplatin-DNA damage responses and enhance platinum accumulation in murine metastatic ovarian cancer xenograft after hyperthermic intraperitoneal chemotherapy (HIPEC). J Ovarian Res. 2011-06-01 [PMID: 21696631] (WB, Human)
• de Feraudy S, Ridd K, Richards LM et al. The DNA damage-binding protein XPC is a frequent target for inactivation in squamous cell carcinomas. Am J Pathol 2010-08-01 [PMID: 20616346] (ICC/IF, Human)
• Acu ID, Liu T, Suino-Powell K et al. Coordination of centrosome homeostasis and DNA repair is intact in MCF-7 and disrupted in MDA-MB 231 breast cancer cells. Cancer Res 2010-04-01 [PMID: 20388771] (ICC/IF, Human)
• Takedachi A, Saijo M, Tanaka K et al. DDB2 complex-mediated ubiquitylation around DNA damage is oppositely regulated by XPC and ku and contributes to the recruitment of XPA. Mol Cell Biol 2010-06-01 [PMID: 20368362] (ICC/IF, Human)
• Rezvani HR, Mahfouf W, Ali N et al. Hypoxia-inducible factor-1alpha regulates the expression of nucleotide excision repair proteins in keratinocytes. Nucleic Acids Res 2010-01-01 [PMID: 19934262] (ICC/IF, Human)
• Yan T, Seo Y, Kinsella TJ et al. Differential cellular responses to prolonged LDR-IR in MLH1-proficient and MLH1-deficient colorectal cancer HCT116 cells. Clin Cancer Res 2009-11-01 [PMID: 19861440] (ICC/IF, Human)
• Klein UR, Nigg EA. SUMO-dependent regulation of centrin-2. J Cell Sci 2009-09-01 [PMID: 19706679] (ICC/IF, Human)
• Wang Y, Liu X, Matsuda A et al. Repair of 2'-C-cyano-2'-deoxy-1-beta-D-arabino-pentofuranosylcytosine-induced DNA single-strand breaks by transcription-coupled nucleotide excision repair. Cancer Res 2008-05-01 [PMID: 18483273] (ICC/IF, Human)
• Ferguson-Yates BE, Li H, Dong TK et al. Impaired repair of cyclobutane pyrimidine dimers in human keratinocytes deficient in p53 and p63. Carcinogenesis 2008-01-01 [PMID: 17984111] (ICC/IF, Human)
• Laposa RR, Huang EJ, Cleaver JE. Increased apoptosis, p53 up-regulation, and cerebellar neuronal degeneration in repair-deficient Cockayne syndrome mice. Proc Natl Acad Sci USA 2007-01-01 [PMID: 17229834] (ICC/IF, Human)
• Lovejoy CA et al. DDB1 maintains genome integrity through regulation of Cdt1. Mol Cell Biol 26(21):7977-90. 2006-11-01 [PMID: 16940174]
• Ferguson BE, Oh DH. Proficient global nucleotide excision repair in human keratinocytes but not in fibroblasts deficient in p53. Cancer Res 2005-10-01 [PMID: 16204041] (ICC/IF, Human)

Review for XPC Antibody (NB100-477) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 07/18/2017

Summary

• Application: Western Blot
• Sample Tested: MEFs whole cell lysate
• Species: Mouse

Bioinformatics

• Gene Symbol: XPC
• Entrez:
  • 7508(Human)
• Uniprot:
  • Q01831(Human)