XPC Antibody (3.26)

Images

 
Western Blot: XPC Antibody (3.26) [NB100-477] - Whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with XPC antibody [3.26] diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: XPC Antibody (3.26) [NB100-477] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: XPC protein stained by XPC antibody [3.26] diluted at 1:100.Red: ...read more
Western Blot: XPC Antibody (3.26) [NB100-477] - HeLa whole cell lysate.
Immunocytochemistry/ Immunofluorescence: XPC Antibody (3.26) [NB100-477] - Left: HeLa cells stained with XPC (3.26) monoclonal antibody (NB100-477). Right: DAPI Staining Photo is kindly provided by Dr. S. Liu at U of ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
3.26
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.15 mg/ml

XPC Antibody (3.26) Summary

Immunogen
Recombinant XPC purified from E. coli
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
XPC
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
WB: Detects an approx. 113 kDa.
Positive Control
Raji Lysate (NB820-59466)
Reviewed Applications
Read 1 Review rated 5
using
NB100-477 in the following applications:

Publications
Read Publications using
NB100-477 in the following applications:

  • 11 publications
  • WB
    3 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 17229834). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.15 mg/ml
Purity
Protein G purified

Alternate Names for XPC Antibody (3.26)

  • complementation group C antibody
  • p125
  • RAD4
  • Xeroderma pigmentosum group C-complementing protein
  • xeroderma pigmentosum, complementation group C
  • XP3
  • XPCCDNA repair protein complementing XP-C cells

Background

Human XPC (Xeroderma pigmentosum group C) is a member of a family of proteins that has been shown to be involved in the repair of DNA via the nucleotide excision repair (NER) pathway. Specifically, XPC is believed to be a part of a heteromeric protein complex that is involved in the recognition of the DNA lesions during global genomic repair but not transcription-coupled repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for XPC Antibody (NB100-477)(16)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(11)
WB
(3)
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Human
(14)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB100-477 Applications Species
Beresova L, Vesela E, Chamrad I et al. Role of DNA Repair Factor Xeroderma Pigmentosum Protein Group C in Response to Replication Stress As Revealed by DNA Fragile Site Affinity Chromatography and Quantitative Proteomics. J. Proteome Res. Dec 2 2016 [PMID: 27794614]
Mohni KN, Kavanaugh GM, Cortez D. ATR pathway inhibition is synthetically lethal in cancer cells with ERCC1 deficiency. Cancer Res. 3/24/2014 [PMID: 24662920] (WB, Human) WB Human
Maria Berra C, Santos de Oliveira C, Carriao Machado Garcia C et al. Nucleotide excision repair activity on DNA damage induced by photoactivated methylene blue. Free Radic Biol Med 2013 Apr 5 [PMID: 23567189] (WB, Human) WB Human
Kothandapani A, Gopalakrishnan K, Kahali B et al. Downregulation Of Swi/Snf Chromatin Remodeling Factor Subunits Modulate Cisplatin Cytotoxicity Exp Cell Res 2012 Jun 18 [PMID: 22721696] (ICC/IF, Human) ICC/IF Human
Muenyi CS, States VA, Masters JH et al. Sodium arsenite and hyperthermia modulate cisplatin-DNA damage responses and enhance platinum accumulation in murine metastatic ovarian cancer xenograft after hyperthermic intraperitoneal chemotherapy (HIPEC). J Ovarian Res. Jun. 2011 [PMID: 21696631] (WB, Human) WB Human
de Feraudy S, Ridd K, Richards LM et al. The DNA damage-binding protein XPC is a frequent target for inactivation in squamous cell carcinomas. Am J Pathol 2010 Aug [PMID: 20616346] (ICC/IF, Human) ICC/IF Human
Acu ID, Liu T, Suino-Powell K et al. Coordination of centrosome homeostasis and DNA repair is intact in MCF-7 and disrupted in MDA-MB 231 breast cancer cells. Cancer Res 2010 Apr [PMID: 20388771] (ICC/IF, Human) ICC/IF Human
Takedachi A, Saijo M, Tanaka K et al. DDB2 complex-mediated ubiquitylation around DNA damage is oppositely regulated by XPC and ku and contributes to the recruitment of XPA. Mol Cell Biol 2010 Jun [PMID: 20368362] (ICC/IF, Human) ICC/IF Human
Rezvani HR, Mahfouf W, Ali N et al. Hypoxia-inducible factor-1alpha regulates the expression of nucleotide excision repair proteins in keratinocytes. Nucleic Acids Res 2010 Jan [PMID: 19934262] (ICC/IF, Human) ICC/IF Human
Yan T, Seo Y, Kinsella TJ et al. Differential cellular responses to prolonged LDR-IR in MLH1-proficient and MLH1-deficient colorectal cancer HCT116 cells. Clin Cancer Res 2009 Nov [PMID: 19861440] (ICC/IF, Human) ICC/IF Human
Show All 16 Publications.

Review for XPC Antibody (NB100-477) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB100-477:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot XPC NB100-477
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reviewed by:
Darshil Patel
WB Mouse 07/18/2017
View

Summary

ApplicationWestern Blot
Sample TestedMEFs whole cell lysate
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for XPC Antibody (NB100-477) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-477AF405
Alexa Fluor 488 Labeled NB100-477AF488
Alexa Fluor 647 Labeled NB100-477AF647
Alexa Fluor 700 Labeled NB100-477AF700
DyLight 350 Labeled NB100-477UV
DyLight 405 Labeled NB100-477V
DyLight 488 Labeled NB100-477G
DyLight 680 Labeled NB100-477FR
DyLight 755 Labeled NB100-477IR

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Bioinformatics Tool for XPC Antibody (NB100-477)

Discover related pathways, diseases and genes to XPC Antibody (NB100-477). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for XPC Antibody (NB100-477)

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Pathways for XPC Antibody (NB100-477)

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Blogs on XPC

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Recent Reviews

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5
1
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2
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1
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Darshil Patel
07/18/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol XPC
Entrez
Uniprot