FEN-1 Antibody (4E7)

Images

 
Western Blot: FEN-1 Antibody (4E7) [NB100-150] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: FEN-1 Antibody (4E7) [NB100-150] - Analysis of paraformaldehyde-fixed MCF7, using antibody at 1:200 dilution.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IP
Clone
4E7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.73 mg/ml

FEN-1 Antibody (4E7) Summary

Immunogen
Recombinant human FEN-1 protein encoding amino acids 1-380 purified E. coli.
Localization
Nuclear
Specificity
This is specific for FEN-1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FEN1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Application Notes
WB: Predicted molecular weight: 43 kDa.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
FEN-1 Lysate (NBL1-10676)
Publications
Read Publications using
NB100-150 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.73 mg/ml
Purity
Protein G purified

Alternate Names for FEN-1 Antibody (4E7)

  • EC 3.1
  • FEN-1
  • FEN-1hFEN-1
  • flap structure-specific endonuclease 1DNase IV
  • Maturation factor 1
  • maturation factor-1
  • MF1flap endonuclease 1
  • RAD2

Background

FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FEN-1 Antibody (NB100-150)(17)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(13)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB100-150 Applications Species
Jaiswal AS, Armas ML, Izumi T et al. Adenomatous Polyposis Coli Interacts with Flap Endonuclease 1 to Block Its Nuclear Entry and Function Neoplasia 2012 Jun [PMID: 22787431] (WB, Human) WB Human
Ma Y, Liang D, Liu J et al. Prostate Cancer Cell Lines under Hypoxia Exhibit Greater Stem-Like Properties. PLoS ONE 6(12). 2011 [PMID: 22216200]
Tann AW, Boldogh I, Meiss G et al. Apoptosis induced by persistent single-strand breaks in mitochondrial genome: critical role of EXOG (5'-EXO/endonuclease) in their repair. J Biol Chem 2011 Sep [PMID: 21768646] (Human) Human
Jaiswal AS, Narayan S. Assembly of the Base Excision Repair Complex on Abasic DNA and Role of Adenomatous Polyposis Coli on Its Functional Activity. Biochemistry;50(11):1901-9. 2011 Mar 22. [PMID: 21261287]
Guo Z, Zheng L, Xu H et al. Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding. Nat Chem Biol 2010 Oct [PMID: 20729856] (Human) Human
Asagoshi K, Liu Y, Masaoka A et al. DNA polymerase beta-dependent long patch base excision repair in living cells. DNA Repair (Amst) 2010 Feb [PMID: 20006562] (Human) Human
Kong X, Mohanty SK, Stephens J et al. Comparative analysis of different laser systems to study cellular responses to DNA damage in mammalian cells. Nucleic Acids Res 2009 May [PMID: 19357094] (Human) Human
Guo Z, Zheng L, Dai H et al. Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity. Nucleic Acids Res 2009 Jun [PMID: 19336415] (Human) Human
Szczesny B, Tann AW, Longley MJ et al. Long patch base excision repair in mammalian mitochondrial genomes. J Biol Chem 2008 Sep [PMID: 18635552] (Human) Human
Liu P, Qian L, Sung JS et al. Removal of oxidative DNA damage via FEN1-dependent long-patch base excision repair in human cell mitochondria. Mol Cell Biol 2008 Aug [PMID: 18541666] (Human) Human
Show All 17 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FEN-1 Antibody (NB100-150) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-150AF405
Alexa Fluor 488 Labeled NB100-150AF488
Alexa Fluor 647 Labeled NB100-150AF647
Alexa Fluor 700 Labeled NB100-150AF700
DyLight 350 Labeled NB100-150UV
DyLight 405 Labeled NB100-150V
DyLight 488 Labeled NB100-150G
DyLight 680 Labeled NB100-150FR
DyLight 755 Labeled NB100-150IR
PerCP Labeled NB100-150PCP

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Bioinformatics

Gene Symbol FEN1
Entrez
Uniprot