FEN-1 Antibody (4E7)


Western Blot: FEN-1 Antibody (4E7) [NB100-150] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: FEN-1 Antibody (4E7) [NB100-150] - Analysis of paraformaldehyde-fixed MCF7, using antibody at 1:200 dilution.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IP, ChIP

Order Details

FEN-1 Antibody (4E7) Summary

Recombinant human FEN-1 protein encoding amino acids 1-380 purified E. coli.
This is specific for FEN-1.
Protein G purified
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  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
  • Western Blot 1:500-1:3000
Application Notes
Chip, ICC/IF,IP- Assay dependent
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
FEN-1 Overexpression Lysate
Read Publications using
NB100-150 in the following applications:

  • B/N
    1 publication
  • WB
    3 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No Preservative
Protein G purified

Alternate Names for FEN-1 Antibody (4E7)

  • EC 3.1
  • FEN1
  • FEN-1
  • FEN-1hFEN-1
  • flap structure-specific endonuclease 1DNase IV
  • Maturation factor 1
  • maturation factor-1
  • MF1flap endonuclease 1
  • RAD2


FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB
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Applications: ELISA, IHC, IHC-P, KD, ELISA(Cap), S-ELISA, WB
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Applications: ICC/IF, IHC, IHC-P, WB
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Applications: ChIP, ICC/IF, IHC, IP, Simple Western, WB
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Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Rt(-)
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Applications: ICC/IF, IHC, IHC-P, WB
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Species: Hu, Mu
Applications: WB, ICC/IF, IP, ChIP

Publications for FEN-1 Antibody (NB100-150)(20)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: B/N, WB.

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Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NB100-150 Applications Species
Yuan B, Kikuchi H, Li J et al. Cytotoxic Effects of Darinaparsin, a Novel Organic Arsenical, against Human Leukemia Cells International Journal of Molecular Sciences 2023-01-23 [PMID: 36768603] (WB) WB
Zhang J, Chen M, Pang Y et al. Flap endonuclease 1 and DNA-PKcs synergistically participate in stabilizing replication fork to encounter replication stress in glioma cells Journal of experimental & clinical cancer research : CR 2022-04-12 [PMID: 35414100] (WB, Human) WB Human
Zhang J, Jing L, Tan S et al. Inhibition of miR-1193 leads to synthetic lethality in glioblastoma multiforme cells deficient of DNA-PKcs Cell Death Dis 2020-07-30 [PMID: 32732911] (B/N) B/N
Jaiswal AS, Armas ML, Izumi T et al. Adenomatous Polyposis Coli Interacts with Flap Endonuclease 1 to Block Its Nuclear Entry and Function Neoplasia 2012-06-01 [PMID: 22787431] (WB, Human) WB Human
Ma Y, Liang D, Liu J et al. Prostate Cancer Cell Lines under Hypoxia Exhibit Greater Stem-Like Properties. PLoS ONE 6(12). 2011-01-01 [PMID: 22216200]
Tann AW, Boldogh I, Meiss G et al. Apoptosis induced by persistent single-strand breaks in mitochondrial genome: critical role of EXOG (5'-EXO/endonuclease) in their repair. J Biol Chem 2011-09-01 [PMID: 21768646] (Human) Human
Jaiswal AS, Narayan S. Assembly of the Base Excision Repair Complex on Abasic DNA and Role of Adenomatous Polyposis Coli on Its Functional Activity. Biochemistry;50(11):1901-9. 2011-03-22 [PMID: 21261287]
Guo Zhigang, Zheng Li, Xu Hong et al. Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding. Nat Chem Biol. 2010-10-01 [PMID: 20729856] (Human) Human
Asagoshi K, Liu Y, Masaoka A et al. DNA polymerase beta-dependent long patch base excision repair in living cells. DNA Repair (Amst) 2010-02-01 [PMID: 20006562] (Human) Human
Kong X, Mohanty SK, Stephens J et al. Comparative analysis of different laser systems to study cellular responses to DNA damage in mammalian cells. Nucleic Acids Res 2009-05-01 [PMID: 19357094] (Human) Human
Show All 20 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies


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Gene Symbol FEN1