XPD Antibody

Western Blot: XPD Antibody [NBP2-20923] - Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: XPD Antibody [NBP2-20923] - ES2 xenograft, using ERCC2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

ERCC2 antibody [N2C2], Internal detects ERCC2 protein at cytosol and nucleus on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. ERCC2 antibody [N2C2], Internal (NBP2-20923) dilution: 1:500. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu, Mu, Rt, Bv, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

XPD Antibody Summary

• Description: Novus Biologicals Rabbit XPD Antibody (NBP2-20923) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human XPD. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rat (99%), Bovine (98%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ERCC2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 87 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for XPD Antibody

• Basic transcription factor 2 80 kDa subunit
• BTF2 p80
• COFS2
• CXPD
• DNA excision repair protein ERCC-2
• DNA repair protein complementing XP-D cells
• EC 3.6.1
• EC 3.6.4.12
• EM9
• EM9TFIIH basal transcription factor complex 80 kDa subunit
• ERCC2
• excision repair cross-complementing rodent repair deficiency, complementationgroup 2
• MAG
• MGC102762
• MGC126218
• MGC126219
• TFIIH 80 kDa subunit
• TFIIH basal transcription factor complex helicase subunit
• TFIIH basal transcription factor complex helicase XPD subunit
• TTD
• xeroderma pigmentosum complementary group D
• Xeroderma pigmentosum group D-complementing protein
• XPD
• XPDC
• XPDTFIIH p80

Background

The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ERCC2
• Uniprot:
  • P18074()