Using Aflatoxin B1 Antibody for Hepatocellular Carcinoma Studies

Thu, 05/13/2010 - 07:29

We at Novus Biologicals are constantly updating our antibody catalog in order to provide as comprehensive a database as possible for molecular biology researchers. Not all our antibodies are derived from proteins found in mammalian or human tissue. Some are derived from single-celled or non-eucaryotic organisms which produce harmful effects when introduced to humans or animals.

This is the case with aflatoxin antibodies. Aflatoxin is a naturally occurring fungal toxin produced by the Aspergillus moulds A. flavus and A. parasiticus. At least 13 aflatoxins are known to exist in nature, with aflatoxin B1 being the most toxic to humans. Unfortunately, the A. flavus spores which produce the toxin are widely found in human habitats, commonly where grain is grown under poor conditions such as drought.

Immunohistochemistry: Aflatoxin B1 Antibody Immunohistochemistry: Aflatoxin B1 Antibody

Aflatoxin B1 antibody is of interest to cancer research groups, as it is thought to be a cause of hepatocellular carcinoma (HCC) to those exposed to the toxin in, for example, agricultural and grain processing environments. This risk is increased where changes in hepatic DNA occur. In November 2009, Long, et. al. reported that polymorphisms in the XPD (xeroderma pigmentosum) gene could influence the DNA repair following exposure to AFB1 (aflatoxin B1), thus increasing the risk of hepatocellular tumors. The study focused on the 312 and 751 XPD codons, which are commonly associated with nucleotide excision repair.

In the experiment, case-control studies, using TaqMan-PCR and and PCR-RFLP antibody analysis, were conducted on the Guangxi population of China. HCC and control patients were used. It was discovered that the HCC risk was raised in those subjects exhibiting the relevant XPD genotype at codon 751. Codon 312 alleles had no effect.

Novus Biologicals offers many Aflatoxin B1 reagents for your research needs including:


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