XPD Antibody (S08-2I3)

Western Blot: XPD Antibody (S08-2I3) [NBP3-15112] - Western blot detection of XPD in Jurkat, Hela cell lysates using XPD Rabbit mAb (1:500 diluted). Predicted band size: 87kDa. Observed band size: 80kDa.

Immunocytochemistry/Immunofluorescence: XPD Antibody (S08-2I3) [NBP3-15112] - Immunocytochemistry of XPD (green) in Hela using NBP3-15112 at dilution 1/50, and DAPI (blue)

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: S08-2I3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

XPD Antibody (S08-2I3) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human XPD (Uniprot#: P18074)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ERCC2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1/100
  • Western Blot 1/1000-1/5000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for XPD Antibody (S08-2I3)

• Basic transcription factor 2 80 kDa subunit
• BTF2 p80
• COFS2
• CXPD
• DNA excision repair protein ERCC-2
• DNA repair protein complementing XP-D cells
• EC 3.6.1
• EC 3.6.4.12
• EM9
• EM9TFIIH basal transcription factor complex 80 kDa subunit
• ERCC2
• excision repair cross-complementing rodent repair deficiency, complementationgroup 2
• MAG
• MGC102762
• MGC126218
• MGC126219
• TFIIH 80 kDa subunit
• TFIIH basal transcription factor complex helicase subunit
• TFIIH basal transcription factor complex helicase XPD subunit
• TTD
• xeroderma pigmentosum complementary group D
• Xeroderma pigmentosum group D-complementing protein
• XPD
• XPDC
• XPDTFIIH p80

Background

The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERCC2