xCT Antibody

Images

 
Western Blot: xCT Antibody [NB300-317] - Detection of XCT in total human stomach lysate using NB 300-317. 15 minute ECL exposure.
Immunocytochemistry/ Immunofluorescence: xCT Antibody [NB300-317] - xCT antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

xCT Antibody Summary

Immunogen
A synthetic peptide made to a region within the N-terminus of the mouse xCT protein (between residues 1-50). [UniProt# Q9WTR6]
Predicted Species
Mouse (100%), Rat (94%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC7A11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Application Notes
This xCT antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band is observed at ~55 kDa; in ICC/IF membrane and ER staining was visualized in HepG2 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Stomach Lysate (NB820-59260)
Control Peptide
xCT Peptide (NB300-317PEP)
Publications
Read Publications using
NB300-317 in the following applications:

Reactivity Notes

Human. Predicted to react with mouse and canine based on 100% sequence homology. Immunogen sequence has 94% homology to rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for xCT Antibody

  • Amino acid transport system xc-
  • Calcium channel blocker resistance protein CCBR1
  • CCBR1
  • cystine/glutamate transporter
  • Member 11 (Slc7a11)
  • SLC7A11
  • Solute carrier family 7 member 11
  • solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
  • xCT
  • xCTCCBR1

Background

System xc- is a cystine-glutamate exchange transporter that is composed of a light-chain subunit (xCT, SLC7A11) as well as a heavy-chain subunit (CD98hc, SLC3A2), and the xCT expression levels correlate with system xc-activity. SLC7A11 gene encodes xCT which is a multipass membrane protein with 12 transmembrane domains having both termini located intracellularly. xCT is widely expressed in various tissues and exert multiple roles such as pheomelanin production, cellular proliferation/metastasis, Kaposi's sarcoma herpesvirus (KSHV) entry into the host cells, learning and memory. Because cystine absorbed by cells via system xc- is rapidly reduced to cysteine for incorporation into GSH, the cell surface xCT expression is considered an important determinant of intracellular redox balance. Most cells require little cystine uptake to sustain cellular protein biosynthesis and GSH production, however this is different in cancer cells which generate high ROS levels via enhanced metabolism and require high GSH levels to survive oxidative stress. Accordingly, xCT is expressed at high levels in various malignant tumors including leukemias, lymphomas, Karposi's sarcoma, pancreatic cancer, and brain cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF

Publications for xCT Antibody (NB300-317)(4)

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Product General Protocols

View specific protocols for xCT Antibody (NB300-317): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for xCT Antibody (NB300-317) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-317AF405
Alexa Fluor 488 Labeled NB300-317AF488
Alexa Fluor 647 Labeled NB300-317AF647
Alexa Fluor 700 Labeled NB300-317AF700
Biotin Labeled NB300-317B
DyLight 405 Labeled NB300-317V
DyLight 488 Labeled NB300-317G
DyLight 550 Labeled NB300-317R
DyLight 650 Labeled NB300-317C
FITC Labeled NB300-317F
HRP Labeled NB300-317H

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Bioinformatics Tool for xCT Antibody (NB300-317)

Discover related pathways, diseases and genes to xCT Antibody (NB300-317). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for xCT Antibody (NB300-317)

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Pathways for xCT Antibody (NB300-317)

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Research Areas for xCT Antibody (NB300-317)

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Blogs on xCT.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

xCT: The Membrane's Gatekeeper
xCT is an obligate, electroneutral, membrane-bound anionic transporter responsible for regulating particular amino acid gradients via their transport through plasma membrane. The antiporter xCT superficially resembles an ion channel and preferentially...  Read full blog post.

xCT: Amino Acid Transport and Disorders of the Central Nervous System
xCT, encoded by the gene SLC7A11, is a member of the heterodimeric amino acid transporter family. Proteins within this family are linked to one another via a disulphide bond to form heterodimers consisting of one light subunit and one heavy subunit (1...  Read full blog post.

xCT: Friend or Foe?
There are two opposing sides to the controversial cysteine/glutamate antiporter. On one hand, it can be viewed a guardian of the cell, protecting it from the damaging oxidative stress that can cause cell death and even cancer. But, conversely, it has...  Read full blog post.

Glutathione and xCT: Chemoresistance in Tumor Cells
Glutathione, called GSH in its reduced form and GSSG or L(-)-Glutathione in its oxidized form, is an endogenous antioxidant found in most cells in the body. Glutathione's functions include detoxifying xenobiotics from the body, assisting in membrane t...  Read full blog post.

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Bioinformatics

Gene Symbol SLC7A11
Entrez