xCT Antibody - BSA Free

Images

 
Western Blot: xCT Antibody [NB300-317] - Total protein from human HeLa, Huvec, HCT-116 and A549 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Immunohistochemistry-Paraffin: xCT Antibody [NB300-317] - xCT expression is enhanced in CD68+ cells from MS spinal cord. CD68+ cells (arrows) show enhanced xCT expression in MS patients as compared to controls. CD68+ ...read more
Biological Strategies: Western Blot: xCT Antibody [NB300-317] - Total protein from Human HeLa cells treated with and without 0.1 mM Diethyl Maleate for 24 hours was separated on a 12% gel by SDS-PAGE, transferred ...read more
Flow Cytometry: xCT Antibody [NB300-317] - An intracellular stain was performed on HeLa cells with NB300-317G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% ...read more
Immunocytochemistry/ Immunofluorescence: xCT Antibody [NB300-317] - xCT antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Flow Cytometry: xCT Antibody [NB300-317] - An intracellular stain was performed on HeLa with NB300-317 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were ...read more
Simple Western: xCT Antibody [NB300-317] - (1) ladder, (2) no lysate + xCT, 100ug/ml, (3) human brain frontal cortex membrane lysate,no primary antibody, (4-7) human brain lysates, 0.03mg/ml, xCT, 100ug/ml.
Analysis of a FFPE tissue section of human stomach using 1:200 dilution of xCT antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

xCT Antibody - BSA Free Summary

Immunogen
This xCT Antibody was prepared from a synthetic peptide made to a region within the N-terminus of the mouse xCT protein (between residues 1-50). [Uniprot: Q9WTR6]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC7A11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1 - 5 ug/ml
  • Immunoblotting reported in scientific literature (PMID 28185919)
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:1000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:10-1:500. Use reported in scientific literature (PMID 21639880)
  • Immunohistochemistry-Paraffin 1:200
  • Simple Western
  • Western Blot 0.5-2 ug/ml
Application Notes
In Western blot, a band is observed at ~35 kDa. In ICC/IF, membrane and ER staining was visualized in HepG2 cells.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Stomach Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
xCT Protein (NB300-317PEP)
Reviewed Applications
Read 1 Review rated 4
using
NB300-317 in the following applications:

Publications
Read Publications using
NB300-317 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: orangutan (82%). Mouse reactivity reported in scientific literature (PMID: 30279737).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for xCT Antibody - BSA Free

  • Amino acid transport system xc-
  • Calcium channel blocker resistance protein CCBR1
  • CCBR1
  • cystine/glutamate transporter
  • SLC7A11
  • solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11
  • Solute carrier family 7 member 11
  • solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
  • xCT

Background

xCT, also called SLC7A11, is the light chain component of the cysteine/glutamate amino acid exchange transporter system Xc (1,2). System Xc is composed of two subunits, the light chain (xCT) and the heavy chain (CD98hc, SLC3A2) and functions by cellular uptake of cysteine in exchange for glutamate in a 1:1 ratio (1,2). The human xCT gene is located on chromosome 4q28.3 and is synthesized as a 12-pass transmembrane protein with both the N- and C-terminals located intracellularly (2, 3). xCT is a 501 amino acids (aa) protein with a theoretical molecular weight of 55.4 kDa (3, 4). xCT expression serves many functional purposes in cells including redox balance, ferroptosis, and chemotherapy or cancer drug resistance (1-3, 5-7). Import of cysteine by xCT plays a role in promoting oxidative stress response as cysteine is a precursor for glutathione synthesis (2, 3, 5-7). Glutathione is a cofactor for ROS-detoxifying enzymes, including glutathione peroxidase (GPX), which help defend from cellular ROS-induced damage (2, 3, 5-7). In addition to its antioxidant role, xCT also utilizes glutathione and GPX to inhibit ferroptosis, which is iron-dependent, non-apoptotic cell-death that occurs with overproduction of lipid hydroperoxides (1-3, 5-7). As cancer cells often experience high oxidative stress, it is understandable that xCT is overexpressed in a variety of cancer types, such as acute myeloid leukemia and breast cancer, and affects cancer growth, invasion, metastasis, and prognosis (1-3, 5-7). xCT expression has also been shown to play a role in glutathione-mediated drug resistance during cancer treatment (1,5,7). However, studies have shown that xCT knockdown results in increased tumor cell death, highlighting its suitability as a druggable target (1,5,7). Specifically, the xCT inhibitors Sulfasalazine, an approved anti-inflammatory drug, and Erastin, a small molecule inhibitor, are potential therapeutic modalities for treating a variety of cancers when used in combination with radiotherapy or immunotherapy (1-3, 5-7).

References

1. Liu, J., Xia, X., & Huang, P. (2020). xCT: A Critical Molecule That Links Cancer Metabolism to Redox Signaling. Molecular therapy : the journal of the American Society of Gene Therapy. https://doi.org/10.1016/j.ymthe.2020.08.021

2. Koppula, P., Zhang, Y., Zhuang, L., & Gan, B. (2018). Amino acid transporter SLC7A11/xCT at the crossroads of regulating redox homeostasis and nutrient dependency of cancer. Cancer communications. https://doi.org/10.1186/s40880-018-0288-x

3. Lin, W., Wang, C., Liu, G., Bi, C., Wang, X., Zhou, Q., & Jin, H. (2020). SLC7A11/xCT in cancer: biological functions and therapeutic implications. American journal of cancer research.

4. xCT: Uniprot (Q9UPY5)

5. Koppula, P., Zhuang, L., & Gan, B. (2020). Cystine transporter SLC7A11/xCT in cancer: ferroptosis, nutrient dependency, and cancer therapy. Protein & cell. https://doi.org/10.1007/s13238-020-00789-5

6. Liu, L., Liu, R., Liu, Y., Li, G., Chen, Q., Liu, X., & Ma, S. (2020). Cystine-glutamate antiporter xCT as a therapeutic target for cancer. Cell biochemistry and function. https://doi.org/10.1002/cbf.3581

7. Cui, Q., Wang, J. Q., Assaraf, Y. G., Ren, L., Gupta, P., Wei, L., Ashby, C. R., Jr, Yang, D. H., & Chen, Z. S. (2018). Modulating ROS to overcome multidrug resistance in cancer. Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy. https://doi.org/10.1016/j.drup.2018.11.001

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for xCT Antibody (NB300-317)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: B/N, IHC-Fr, IHC-P, WB.


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B/N
(1)
IHC-Fr
(2)
IHC-P
(1)
WB
(4)
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Human
(3)
Mouse
(3)
Rat
(2)
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Showing Publications 1 - 10 of 10.
Publications using NB300-317 Applications Species
Wang J, Wang Y, Liu Y et al. Ferroptosis, a new target for treatment of renal injury and fibrosis in a 5/6 nephrectomy-induced CKD rat model Cell death discovery 2022-03-22 [PMID: 35318301] (IHC-P, Rat) IHC-P Rat
Zhang HF, Hughes CS, Li W et al. Proteomic Screens for Suppressors of Anoikis Identify IL1RAP as a Promising Surface Target in Ewing Sarcoma Cancer Discovery 2021-11-01 [PMID: 34021002]
Martinez R 3rd, Huang W, Samadani R et al. Mechanistic Analysis of an Extracellular Signal-Regulated Kinase 2-Interacting Compound that Inhibits Mutant BRAF-Expressing Melanoma Cells by Inducing Oxidative Stress Journal of Pharmacology and Experimental Therapeutics 2021-01-01 [PMID: 33109619] (B/N) B/N
Long Y, Tao H, Karachi A et al. Dysregulation of glutamate transport enhances Treg function that promotes VEGF blockade resistance in glioblastoma Cancer Res. 2019-11-13 [PMID: 31723000] (IHC-Fr, Mouse) IHC-Fr Mouse
Huang M, Lin Y, Chang C et al. RGS4 deficit in prefrontal cortex contributes to the behaviors related to schizophrenia via system xc--mediated glutamatergic dysfunction in mice. Theranostics 2018-09-13 [PMID: 30279737] (WB, Mouse) WB Mouse
Chew SH, Okazaki Y, Akatsuka S et al. Rheostatic CD44 isoform expression and its association with oxidative stress in human malignant mesothelioma. Free Radic. Biol. Med. 2017-02-06 [PMID: 28185919]
Linher-Melville K, Haftchenary S, Gunning P et al. Signal transducer and activator of transcription 3 and 5 regulate system Xc- and redox balance in human breast cancer cells. Mol. Cell. Biochem. 2015-04-21 [PMID: 25896132] (WB, Human) WB Human
Pampliega O, Domercq M, Soria FN et al. Increased expression of cystine/glutamate antiporter in multiple sclerosis. J Neuroinflammation. 2011-06-01 [PMID: 21639880] (IHC-Fr, Rat, Human) IHC-Fr Rat, Human
Liu, R et al. Cystine-glutamate transporter SLC7A11 mediates resistance to geldanamycin but not to 17-(allylamino)-17-demethoxygeldanamycin. Mol Pharmacol;72(6):1637-46. 2007-12-01 [PMID: 17875604] (WB, Human) WB Human
Davidson-Hunt A Supplemental glutamine to restore glutathione homeostasis in the aging mouse liver Thesis 2022-01-01 (WB, Mouse)

Details:
Dilution used in WB 1:500
WB Mouse

Review for xCT Antibody (NB300-317) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB300-317:
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Simple Western
(1)
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Images Ratings Applications Species Date Details
Simple Western xCT NB300-317
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4
reviewed by:
Diane Brooks
Simple Western Human 01/18/2018
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Summary

ApplicationSimple Western
Sample TestedHuman Brain Tissue,human glioblastoma
SpeciesHuman
LotD1

Comments

CommentsMembrane lysates were extracted using ThermoFisher Mem-PER Plus Membrane Protein Extraction Kit Cat# 89842. Samples were reduced following Simple Western kit instructions in 40mM DTT for 5 minutes at 95oC and separated using the 12-230kDa separation kit.
Primary antibody followed by HRP-conjugated Anti-Rabbit IgG secondary antibody (Protein Simple Cat # 042-206).

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for xCT Antibody (NB300-317). (Showing 1 - 1 of 1 FAQs).

  1. Do you have a slc7a11 antibody that is conjugated, reacts to mouse, works with cell surface staining, does not need permeabilization?
    • SLC7A11 or xCT antibody NB300-318AF594 targets a cytoplasmic region of the protein, so it should work without permeabilization. However, the data does say it was permeabilized with saponin, so we cannot say for certain if it will work without permeabilization.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Array NB300-317

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Research Areas for xCT Antibody (NB300-317)

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Blogs on xCT.

The effects of ethanol consumption on glutamate production and xCT
xCT is a sodium independent glutamate transporter that regulates the exchange of extracellular l-cystine and intracellular l-glutamate across the plasma membrane. This process is critical to glutathione production and protection from subsequent ox...  Read full blog post.

xCT: The Membrane's Gatekeeper
xCT is an obligate, electroneutral, membrane-bound anionic transporter responsible for regulating particular amino acid gradients via their transport through plasma membrane. The antiporter xCT superficially resembles an ion channel and preferentially...  Read full blog post.

xCT: Amino Acid Transport and Disorders of the Central Nervous System
xCT, encoded by the gene SLC7A11, is a member of the heterodimeric amino acid transporter family. Proteins within this family are linked to one another via a disulphide bond to form heterodimers consisting of one light subunit and one heavy subunit (1...  Read full blog post.

xCT: Friend or Foe?
There are two opposing sides to the controversial cysteine/glutamate antiporter. On one hand, it can be viewed a guardian of the cell, protecting it from the damaging oxidative stress that can cause cell death and even cancer. But, conversely, it has ...  Read full blog post.

Glutathione and xCT: Chemoresistance in Tumor Cells
Glutathione, called GSH in its reduced form and GSSG or L(-)-Glutathione in its oxidized form, is an endogenous antioxidant found in most cells in the body. Glutathione's functions include detoxifying xenobiotics from the body, assisting in membrane t...  Read full blog post.

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Diane Brooks
01/18/2018
Application: Simple Western
Species: Human

Bioinformatics

Gene Symbol SLC7A11