Western Blot: xCT Antibody [NB300-317] - Detection of XCT in total human stomach lysate using NB 300-317. 15 minute ECL exposure.
Immunocytochemistry/ Immunofluorescence: xCT Antibody [NB300-317] - xCT antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
This xCT antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band is observed at ~55 kDa; in ICC/IF membrane and ER staining was visualized in HepG2 cells.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human. Predicted to react with mouse and canine based on 100% sequence homology. Immunogen sequence has 94% homology to rat.
Alternate Names for xCT Antibody
Amino acid transport system xc-
Calcium channel blocker resistance protein CCBR1
Member 11 (Slc7a11)
Solute carrier family 7 member 11
solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
System xc- is a cystine-glutamate exchange transporter that is composed of a light-chain subunit (xCT, SLC7A11) as well as a heavy-chain subunit (CD98hc, SLC3A2), and the xCT expression levels correlate with system xc-activity. SLC7A11 gene encodes xCT which is a multipass membrane protein with 12 transmembrane domains having both termini located intracellularly. xCT is widely expressed in various tissues and exert multiple roles such as pheomelanin production, cellular proliferation/metastasis, Kaposi's sarcoma herpesvirus (KSHV) entry into the host cells, learning and memory. Because cystine absorbed by cells via system xc- is rapidly reduced to cysteine for incorporation into GSH, the cell surface xCT expression is considered an important determinant of intracellular redox balance. Most cells require little cystine uptake to sustain cellular protein biosynthesis and GSH production, however this is different in cancer cells which generate high ROS levels via enhanced metabolism and require high GSH levels to survive oxidative stress. Accordingly, xCT is expressed at high levels in various malignant tumors including leukemias, lymphomas, Karposi's sarcoma, pancreatic cancer, and brain cancer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
xCT: The Membrane's Gatekeeper xCT is an obligate, electroneutral, membrane-bound anionic transporter responsible for regulating particular amino acid gradients via their transport through plasma membrane. The antiporter xCT superficially resembles an ion channel and preferentially... Read full blog post.
xCT: Friend or Foe? There are two opposing sides to the controversial cysteine/glutamate antiporter. On one hand, it can be viewed a guardian of the cell, protecting it from the damaging oxidative stress that can cause cell death and even cancer. But, conversely, it has... Read full blog post.