CHAF1A Antibody (SS 1 1-13)

Images

 
Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.
Immunocytochemistry/ Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Immunofluorescent staining of CAF-1-p150 in HeLa cells (Shibahara et al 1999).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clone
SS 1 1-13
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CHAF1A Antibody (SS 1 1-13) Summary

Immunogen
Recombinant human CHAF1A [UniProt# Q13111]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CHAF1A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
This CHAF1A (SS 1 1-13) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot, where a band at 150 kDa is seen.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CHAF1A Overexpression Lysate
Publications
Read Publications using
NB500-207 in the following applications:

  • 3 publications
  • IP
    2 publications
  • WB
    1 publication

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for CHAF1A Antibody (SS 1 1-13)

  • CAF
  • CAF-1 subunit A
  • CAF1
  • CAF-1
  • CAF1B
  • CAF1P150CAF-I 150 kDa subunit
  • CAF-I p150
  • chromatin assembly factor 1 subunit A
  • chromatin assembly factor 1, subunit A (p150)
  • chromatin assembly factor I (150 kDa)
  • Chromatin assembly factor I p150 subunit
  • hp150
  • MGC71229
  • P150

Background

Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CHAF1A Antibody (NB500-207)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(3)
IP
(2)
WB
(1)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB500-207 Applications Species
Cohen C, Corpet A, Maroui MA et al. Promyelocytic Leukemia (PML) Nuclear Bodies (NBs) Induce Latent/Quiescent HSV-1 Genomes Chromatinization Through a PML-NB/Histone H3.3/H3.3 Chaperone Axis. bioRxiv [PMID: 30235352] (ICC/IF, Human) ICC/IF Human
Rivera C, Saavedra F, Alvarez F et al. Methylation of histone H3 lysine 9 occurs during translation. Nucleic Acids Res. 2015 Sep 24 [PMID: 26405197] (WB, Human) WB Human
Galvani A, Courbeyrette R, Agez M et al. In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells. Mol Cell Biol;28(11):3672-85. 2008 Jun. [PMID: 18378699]
Ghosh JC et al. Hsp60 regulation of tumor cell apoptosis. J Biol Chem;283(8):5188-94. 2008 Feb 22. [PMID: 18086682]
Mocquet V, Laine JP, Riedl T et al. Sequential recruitment of the repair factors during NER: the role of XPG in initiating the resynthesis step. EMBO J 2008 Jan 9 [PMID: 18079701] (Human) Human
Naryzhny, S et al. Proliferating cell nuclear antigen (PCNA) may function as a double homotrimer complex in the mammalian cell. J Biol Chem;280(14):13888-94. 2005 Apr 8. [PMID: 15805117] (IP, Human) IP Human
Jiao, R et al. Physical and functional interaction between the Bloom's syndrome gene product and the largest subunit of chromatin assembly factor 1. Mol Cell Biol;24(11):4710-9. 2004 Jun. [PMID: 15143166]
Green, CM, Almouzni, G. Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo. EMBO J;22(19):5163-74. 2003 Oct 1. [PMID: 14517254] (ICC/IF, Human) ICC/IF Human
Shibahara, K et al. Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. Cell;96(4):575-85. 1999 Feb 19. [PMID: 10052459] (IP, ICC/IF, Human) IP, ICC/IF Human

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Product General Protocols

View specific protocols for CHAF1A Antibody (NB500-207): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CHAF1A
Entrez
Uniprot