CHAF1A Antibody (SS 1 1-13) - BSA Free

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Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.
Immunocytochemistry/ Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Immunofluorescent staining of CAF-1-p150 in HeLa cells (Shibahara et al 1999).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clone
SS 1 1-13
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

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Catalog# & Formulation Size Price

CHAF1A Antibody (SS 1 1-13) - BSA Free Summary

Immunogen
Recombinant human CHAF1A [UniProt# Q13111]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CHAF1A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
Application Notes
This CHAF1A (SS 1 1-13) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot, where a band at 150 kDa is seen. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CHAF1A Overexpression Lysate
Publications
Read Publications using
NB500-207 in the following applications:

  • 3 publications
  • IP
    2 publications
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for CHAF1A Antibody (SS 1 1-13) - BSA Free

  • CAF
  • CAF-1 subunit A
  • CAF1
  • CAF-1
  • CAF1B
  • CAF1P150CAF-I 150 kDa subunit
  • CAF-I p150
  • chromatin assembly factor 1 subunit A
  • chromatin assembly factor 1, subunit A (p150)
  • chromatin assembly factor I (150 kDa)
  • Chromatin assembly factor I p150 subunit
  • hp150
  • MGC71229
  • P150

Background

Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CHAF1A Antibody (NB500-207)(12)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(3)
IP
(2)
WB
(1)
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Filter By Species
Human
(6)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB500-207 Applications Species
Dreyer J, Ricci G, van den Berg J et Al. Acute multi-level response to defective de novo chromatin assembly in S-phase Mol Cell 2024-11-05 [PMID: 39536749]
Robert Siddaway, Scott Milos, Étienne Coyaud, Hwa Young Yun, Shahir M. Morcos, Sanja Pajovic, Eric I. Campos, Brian Raught, Cynthia Hawkins The in v ivo Interaction Landscape of Histones H3.1 and H3.3 Molecular & Cellular Proteomics : MCP 2022-09-09 [PMID: 36089195]
Cohen C, Corpet A, Maroui MA et al. Promyelocytic Leukemia (PML) Nuclear Bodies (NBs) Induce Latent/Quiescent HSV-1 Genomes Chromatinization Through a PML-NB/Histone H3.3/H3.3 Chaperone Axis. bioRxiv [PMID: 30235352] (ICC/IF, Human) ICC/IF Human
Rivera C, Saavedra F, Alvarez F et al. Methylation of histone H3 lysine 9 occurs during translation. Nucleic Acids Res. 2015-09-24 [PMID: 26405197] (WB, Human) WB Human
Galvani, A et al. In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells. Mol Cell Biol;28(11):3672-85. 2008-06-01 [PMID: 18378699]
Ghosh JC et al. Hsp60 regulation of tumor cell apoptosis. J Biol Chem;283(8):5188-94. 2008-02-22 [PMID: 18086682]
Mocquet V, Laine JP, Riedl T et al. Sequential recruitment of the repair factors during NER: the role of XPG in initiating the resynthesis step. EMBO J 2008-01-09 [PMID: 18079701] (Human) Human
Naryzhny, S et al. Proliferating cell nuclear antigen (PCNA) may function as a double homotrimer complex in the mammalian cell. J Biol Chem;280(14):13888-94. 2005-04-08 [PMID: 15805117] (IP, Human) IP Human
Jiao, R et al. Physical and functional interaction between the Bloom's syndrome gene product and the largest subunit of chromatin assembly factor 1. Mol Cell Biol;24(11):4710-9. 2004-06-01 [PMID: 15143166]
Green, CM, Almouzni, G. Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo. EMBO J;22(19):5163-74. 2003-10-01 [PMID: 14517254] (ICC/IF, Human) ICC/IF Human
Show All 12 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol CHAF1A
Entrez
Uniprot