Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.
Immunocytochemistry/ Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Immunofluorescent staining of CAF-1-p150 in HeLa cells (Shibahara et al 1999).
This CHAF1A (SS 1 1-13) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot, where a band at 150 kDa is seen. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
150 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified
Alternate Names for CHAF1A Antibody (SS 1 1-13)
CAF
CAF-1 subunit A
CAF1
CAF-1
CAF1B
CAF1P150CAF-I 150 kDa subunit
CAF-I p150
chromatin assembly factor 1 subunit A
chromatin assembly factor 1, subunit A (p150)
chromatin assembly factor I (150 kDa)
Chromatin assembly factor I p150 subunit
hp150
MGC71229
P150
Background
Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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