CHAF1A Antibody (SS 1 1-13)

Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.

Immunocytochemistry/Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Immunofluorescent staining of CAF-1-p150 in HeLa cells (Shibahara et al 1999).

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clone: SS 1 1-13
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CHAF1A Antibody (SS 1 1-13) Summary

• Immunogen: Recombinant human CHAF1A [UniProt# Q13111]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHAF1A
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
• Application Notes: This CHAF1A (SS 1 1-13) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot, where a band at 150 kDa is seen. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 150 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  CHAF1A Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for CHAF1A Antibody (SS 1 1-13)

• CAF
• CAF-1 subunit A
• CAF1
• CAF-1
• CAF1B
• CAF1P150CAF-I 150 kDa subunit
• CAF-I p150
• chromatin assembly factor 1 subunit A
• chromatin assembly factor 1, subunit A (p150)
• chromatin assembly factor I (150 kDa)
• Chromatin assembly factor I p150 subunit
• hp150
• MGC71229
• P150

Background

Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHAF1A Antibody (NB500-207)(10)

Publications using NB500-207

• Cohen C, Corpet A, Maroui MA et al. Promyelocytic Leukemia (PML) Nuclear Bodies (NBs) Induce Latent/Quiescent HSV-1 Genomes Chromatinization Through a PML-NB/Histone H3.3/H3.3 Chaperone Axis. bioRxiv [PMID: 30235352] (ICC/IF, Human)
• Rivera C, Saavedra F, Alvarez F et al. Methylation of histone H3 lysine 9 occurs during translation. Nucleic Acids Res. 2015-09-24 [PMID: 26405197] (WB, Human)
• Galvani, A et al. In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells. Mol Cell Biol;28(11):3672-85. 2008-06-01 [PMID: 18378699]
• Ghosh JC et al. Hsp60 regulation of tumor cell apoptosis. J Biol Chem;283(8):5188-94. 2008-02-22 [PMID: 18086682]
• Mocquet V, Laine JP, Riedl T et al. Sequential recruitment of the repair factors during NER: the role of XPG in initiating the resynthesis step. EMBO J 2008-01-09 [PMID: 18079701] (Human)
• Naryzhny, S et al. Proliferating cell nuclear antigen (PCNA) may function as a double homotrimer complex in the mammalian cell. J Biol Chem;280(14):13888-94. 2005-04-08 [PMID: 15805117] (IP, Human)
• Jiao, R et al. Physical and functional interaction between the Bloom's syndrome gene product and the largest subunit of chromatin assembly factor 1. Mol Cell Biol;24(11):4710-9. 2004-06-01 [PMID: 15143166]
• Green, CM, Almouzni, G. Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo. EMBO J;22(19):5163-74. 2003-10-01 [PMID: 14517254] (ICC/IF, Human)
• Shibahara, K, Stillman, B. Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. Cell;96(4):575-85. 1999-02-19 [PMID: 10052459] (ICC/IF, IP, Human)
• Yun HYH Molecular Characterization of Oncogenic H3. 3 K27M and G34R Histone Mutants Thesis 2021-01-01

Bioinformatics

• Gene Symbol: CHAF1A
• Entrez:
  • 10036(Human)
• Uniprot:
  • Q13111(Human)