PIK3R4 Antibody

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Independent Antibodies: Western Blot: PIK3R4 Antibody [NBP1-30463] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. Vps15 was also immunoprecipitated by rabbit anti-Vps15 antibody NBP1-30462.

Product Details

Summary
Reactivity Hu, Mu, Ze, RtSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

PIK3R4 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 1300 and 1358 of human vacuolar protein sorting 15 using the numbering given in entry NP_055417.1 (GeneID 30849).
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIK3R4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24089209)
Theoretical MW
153 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-30463 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    3 publications

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan. Zebrafish reactivity reported in scientific literature (PMID: 27882921)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PIK3R4 Antibody

  • EC 2.7.11.1
  • MGC102700
  • p150
  • phosphatidylinositol 3-kinase-associated p150
  • Phosphoinositide 3-kinase adaptor protein
  • phosphoinositide 3-kinase regulatory subunit 4
  • phosphoinositide-3-kinase, regulatory subunit 4
  • phosphoinositide-3-kinase, regulatory subunit 4, p150
  • PI3-kinase p150 subunit
  • PI3-kinase regulatory subunit 4
  • PIK3R4
  • VPS 15
  • VPS15

Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PIK3R4 Antibody (NBP1-30463)(5)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PIK3R4
Entrez
Uniprot