PIK3R4 Antibody


Independent Antibodies: Western Blot: PIK3R4 Antibody [NBP1-30463] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. Vps15 was also immunoprecipitated by rabbit anti-Vps15 antibody NBP1-30462.

Product Details

Reactivity Hu, Mu, Rt, Ze, Pm-OrSpecies Glossary
Applications WB, ICC/IF, IP
0.2 mg/ml

Order Details

PIK3R4 Antibody Summary

The immunogen for this product maps to a region between residue 1300 and 1358 of human vacuolar protein sorting 15 using the numbering given in entry NP_055417.1 (GeneID 30849).
Predicted Species
Orangutan (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 24089209). Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Theoretical MW
153 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NBP1-30463 in the following applications:

  • 2 publications
  • IP
    1 publication
  • WB
    5 publications

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25961502). Zebrafish reactivity reported in scientific literature (PMID: 27882921).

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
TBS and 0.1% BSA
0.09% Sodium Azide
0.2 mg/ml
Immunogen affinity purified

Alternate Names for PIK3R4 Antibody

  • EC
  • MGC102700
  • p150
  • phosphatidylinositol 3-kinase-associated p150
  • Phosphoinositide 3-kinase adaptor protein
  • phosphoinositide 3-kinase regulatory subunit 4
  • phosphoinositide-3-kinase, regulatory subunit 4
  • phosphoinositide-3-kinase, regulatory subunit 4, p150
  • PI3-kinase p150 subunit
  • PI3-kinase regulatory subunit 4
  • PIK3R4
  • VPS 15
  • VPS15


Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
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Species: Bv, Ce, ChHa, Hu, Pm, Mu, Rt, RM
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Species: Mu
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Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Mu
Applications: BA
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu
Applications: BA
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, MiAr, WB
Species: Hu, Mu, Po, Rt
Applications: ICC/IF, KD, Simple Western, WB
Species: Hu
Applications: ICC/IF, IP, WB
Species: Hu, Mu
Applications: ICC/IF, KO, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ICC/IF, IP, KD, WB
Species: Hu, Mu, Rt, Ze, Pm-Or
Applications: WB, ICC/IF, IP

Publications for PIK3R4 Antibody (NBP1-30463)(12)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Zebrafish.

We have publications tested in 3 applications: ICC/IF, IP, WB.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP1-30463 Applications Species
Bork T, Liang W, Kretz O et al. BECLIN1 Is Essential for Podocyte Secretory Pathways Mediating VEGF Secretion and Podocyte-Endothelial Crosstalk International journal of molecular sciences 2022-03-30 [PMID: 35409185]
Mercer TJ, Ohashi Y, Boeing S et al. Phosphoproteomic identification of ULK substrates reveals VPS15-dependent ULK/VPS34 interplay in the regulation of autophagy The EMBO journal 2021-06-14 [PMID: 34121209]
Wang R, Simoneau CR, Kulsuptrakul J et al. Genetic Screens Identify Host Factors for SARS-CoV-2 and Common Cold Coronaviruses Cell 2020-12-09 [PMID: 33333024] (WB, Human) WB Human
Meena N, Ralston E, Raben N, Puertollano R Enzyme Replacement Therapy Can Reverse Pathogenic Cascade in Pompe Disease Mol Ther Methods Clin Dev 2020-07-17 [PMID: 32671132]
Franco-Iborra S, Plaza-Zabala A, Montpeyo M et al. Mutant HTT (huntingtin) impairs mitophagy in a cellular model of Huntington disease Autophagy 2020-02-24 [PMID: 32093570]
Gstrein T, Edwards A, PristoupilovA A et al. Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans. Nat. Neurosci. 2018-02-01 [PMID: 29311744] (WB, Human) WB Human
Liu Y, Hu M, Luo D et al. Class III PI3K Positively Regulates Platelet Activation and Thrombosis via PI(3)P-Directed Function of NADPH Oxidase Arterioscler. Thromb. Vasc. Biol. 2017-09-07 [PMID: 28882875] (Mouse) Mouse
Stoetzel C, Bar S, De Craene JO et al. A mutation in VPS15 (PIK3R4) causes a ciliopathy and affects IFT20 release from the cis-Golgi. Nat Commun. 2016-11-24 [PMID: 27882921] (WB, Zebrafish) WB Zebrafish
Bejarano E, Yuste A, Patel B et al. Connexins modulate autophagosome biogenesis. Nat. Cell Biol. 2014-04-06 [PMID: 24705551] (WB, IP, Mouse) WB, IP Mouse
Pampliega O, Orhon I, Patel B et al. Functional interaction between autophagy and ciliogenesis. Nature. 2013-10-10 [PMID: 24089209] (ICC/IF, Mouse) ICC/IF Mouse
Show All 12 Publications.

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ICC/IF Video Protocol

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Gene Symbol PIK3R4