EEA1 Antibody (6D4.1D4)

Western Blot: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of partial recombinant human EEA1 protein (14kDa) with EEA1 antibody clone 6D4.1D4 at 0.5 ug mL.

Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of FFPE tissue section of human testes using mouse monoclonal EEA1 antibody (clone 6D4.1D4) at 10 ug/ml concentration. The interstitial cells depicted a punctate to granular cytoplasmic staining pattern for EEA1 protein.

Flow (Intracellular): EEA1 Antibody (6D4.1D4) [NBP2-36568] - Intracellular staining of human EEA1 in Flow cytometry using 2.5 ug of antibody per 1 million cells. Isotype control was mouse IgG1.

Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of FFPE tissue section of mouse testes using mouse monoclonal EEA1 antibody (clone 6D4.1D4) at 10 ug/ml concentration. The interstitial cells depicted a punctate to granular cytoplasmic staining pattern for EEA1 protein with no signal in other cell types.

• Reactivity: Hu, Mu, V-Vi, Rt, Po, Bt, Pm
• Applications: WB, Flow-IC, IHC, IHC-P
• Clone: 6D4.1D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

EEA1 Antibody (6D4.1D4) Summary

• Immunogen: Partial recombinant human EEA1 protein (between amino acids 50-300). [UniProt Q15075]
• Localization: Cytoplasm. Early endosome membrane.
• Marker: Early Endosome Marker
• Predicted Species: Rat (95%), Porcine (95%), Primate (99%), Bat (93%). Backed by our 100% Guarantee.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EEA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Immunohistochemistry 5-10 ug/mL
  • Immunohistochemistry-Paraffin 5-10 ug/mL
  • Flow (Intracellular) 2.5 ug/million cells
• Control:

  EEA1 Overexpression Lysate

Reactivity Notes

Virus reactivity reported in scientific literature (Lu et al).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for EEA1 Antibody (6D4.1D4)

• early endosome antigen 1
• early endosome antigen 1, 162kD
• early endosome-associated protein
• EEA1
• Endosome-associated protein p162
• MST105
• MSTP105
• ZFYVE2
• ZFYVE2MST105
• Zinc finger FYVE domain-containing protein 2

Background

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc finger like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl terminal domains of EEA1. The carboxyl terminal zinc finger like domain is conserved in several other non nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3 kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EEA1 Antibody (NBP2-36568)(1)

Publication using NBP2-36568

• Lu G, Li S, Zhou C et al. Tenuivirus uses a molecular bridge strategy to overcome insect midgut barriers for virus persistent transmission. bioRxiv Aug 31 2018 (ICC/IF, Virus)

Bioinformatics

• Gene Symbol: EEA1
• Entrez:
  • 8411(Human)
  • 216238(Mouse)
• Uniprot:
  • Q15075(Human)