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EEA1 Antibody (6D4.1D4)

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Western Blot: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of partial recombinant human EEA1 protein (14kDa) with EEA1 antibody clone 6D4.1D4 at 0.5 ug mL.
Western Blot: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of partial recombinant human EEA1 protein (14kDa) with EEA1 antibody clone 6D4.1D4 at 0.5 ug mL.
Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of FFPE tissue section of human testes using mouse monoclonal EEA1 antibody (clone 6D4.1D4) at 10 ug/ml concentration. The interstitial ...read more
Flow (Intracellular): EEA1 Antibody (6D4.1D4) [NBP2-36568] - Intracellular staining of human EEA1 in Flow cytometry using 2.5 ug of antibody per 1 million cells. Isotype control was mouse IgG1.
Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of FFPE tissue section of mouse testes using mouse monoclonal EEA1 antibody (clone 6D4.1D4) at 10 ug/ml concentration. The interstitial ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bt, PmSpecies Glossary
Applications WB, Flow-IC, ICC/IF, IHC, IHC-P
Clone
6D4.1D4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-36568AF350
Alexa Fluor 405
NBP2-36568AF405
Alexa Fluor 488
NBP2-36568AF488
Alexa Fluor 532
NBP2-36568AF532
Alexa Fluor 594
NBP2-36568AF594
Alexa Fluor 647
NBP2-36568AF647
Alexa Fluor 700
NBP2-36568AF700
Alexa Fluor 750
NBP2-36568AF750
Allophycocyanin
NBP2-36568APC
Biotin
NBP2-36568B
DyLight 350
NBP2-36568UV
DyLight 405
NBP2-36568V
DyLight 488
NBP2-36568G
DyLight 550
NBP2-36568R
DyLight 594
NBP2-36568DL594
DyLight 650
NBP2-36568C
DyLight 680
NBP2-36568FR
DyLight 755
NBP2-36568IR
FITC
NBP2-36568F
HRP
NBP2-36568H
Janelia Fluor 549
NBP2-36568JF549
Janelia Fluor 646
NBP2-36568JF646
PE
NBP2-36568PE
PerCP
NBP2-36568PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-80711
100% Guarantee on every product

EEA1 Antibody (6D4.1D4) Summary

Immunogen
Partial recombinant human EEA1 protein (between amino acids 50-300). [UniProt Q15075]
Localization
Cytoplasm. Early endosome membrane.
Marker
Early Endosome Marker
Predicted Species
Rat (95%), Porcine (95%), Bat (93%), Primate (99%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EEA1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Flow (Intracellular) 2.5 ug/million cells
Application Notes
Use in ICC/IF reported in scientific literature (PMID:33212210)
Control
EEA1 Overexpression Lysate
Publications
Read Publications using
NBP2-36568 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for EEA1 Antibody (6D4.1D4)

  • early endosome antigen 1
  • early endosome antigen 1, 162kD
  • early endosome-associated protein
  • EEA1
  • Endosome-associated protein p162
  • MST105
  • MSTP105
  • ZFYVE2
  • ZFYVE2MST105
  • Zinc finger FYVE domain-containing protein 2

Background

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc finger like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl terminal domains of EEA1. The carboxyl terminal zinc finger like domain is conserved in several other non nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3 kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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(1 Publication)
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Applications: WB, ICC/IF, IHC, IHC-P
AKT [p Ser473]
Antibody - Pan Specif
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho‑Akt (S473) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF887) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
(25 Publications)
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Antibody (7C8)
NB100-615
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Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - Subcellular localization of mPARM-1 and hPARM-1 (full-length and mutant proteins). NIH/3T3 cells transiently expressing hPARM-1-GFP or deltaCT-GFP were fixed, immunostained for caveolin-1 (1:100, Novus Biologicals), and examined by confocal fluorescence microscopy. For hPARM-1-GFP-caveolin-1 co-localization, cells that demonstrated cell membrane PARM-1 localization were chosen. All co-localizations were observed following merging images of GFP-tagged proteins with those of Golgi, endosomes, plasma membrane, alpha-tubulin or caveolin-1 labeling. Image collected and cropped by CiteAb from the following publication (http://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-12-84), licensed under a CC-BY licence.
Immunohistochemistry-Frozen: Caveolin-1 Antibody (7C8) [NB100-615] - Immunofluorescence of human adipose tissue. Primary antibody at 1:250. IHC-Fr image submitted by a verified customer review.
(3 Reviews)
(26 Publications)
Species: Hu, Mu, Rt, Sh
Applications: WB, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
EEA1 Antibody
(6D4.1D4)
NBP2-36568
EEA1 Antibody (6D4.1D4)
Species: Hu, Mu, Rt, Po, Bt, Pm
Applications: WB, Flow-IC, ICC/IF, IHC, IHC-P

Publications for EEA1 Antibody (NBP2-36568)(2)

We have publications tested in 2 confirmed species: Human, Virus.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(2)
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Human
(1)
Virus
(1)
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Showing Publications 1 - 2 of 2.
Publications using NBP2-36568 Applications Species
Lu G, Li S, Zhou C et al. Tenuivirus uses a molecular bridge strategy to overcome insect midgut barriers for virus persistent transmission. bioRxiv Aug 31 2018 (ICC/IF, Virus) ICC/IF Virus
Murakami R, Chiba Y, Nishi N et al. Immunoreactivity of receptor and transporters for lactate located in astrocytes and epithelial cells of choroid plexus of human brain Neurosci Lett Nov 16 2020 [PMID: 33212210] (ICC/IF, Human)

Details:
Immunofluorescence analysis of human brain samples. 		ICC/IF Human

Reviews for EEA1 Antibody (NBP2-36568) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EEA1 Antibody (NBP2-36568) (0)

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Control Lysate(s)

EEA1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-36568AF350
Alexa Fluor 405 NBP2-36568AF405
Alexa Fluor 488 NBP2-36568AF488
Alexa Fluor 532 NBP2-36568AF532
Alexa Fluor 594 NBP2-36568AF594
Alexa Fluor 647 NBP2-36568AF647
Alexa Fluor 700 NBP2-36568AF700
Alexa Fluor 750 NBP2-36568AF750
Allophycocyanin NBP2-36568APC
Biotin NBP2-36568B
DyLight 350 NBP2-36568UV
DyLight 405 NBP2-36568V
DyLight 488 NBP2-36568G
DyLight 550 NBP2-36568R
DyLight 594 NBP2-36568DL594
DyLight 650 NBP2-36568C
DyLight 680 NBP2-36568FR
DyLight 755 NBP2-36568IR
FITC NBP2-36568F
HRP NBP2-36568H
Janelia Fluor 549 NBP2-36568JF549
Janelia Fluor 646 NBP2-36568JF646
PE NBP2-36568PE
PerCP NBP2-36568PCP
Learn about Custom Antibody Labeling

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Blogs on EEA1.

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Understanding EEA1's Role in Membrane Endosome Fusion
EEA1, or Early Endosome Antigen 1 is a Rab5 effector essential for early endocytic membrane fusion. The EEA1 antibody is used in membrane trafficking and chaperone studies, and as an endosome marker. We at Novus Biologicals have a comprehensive antibo...  Read full blog post.
EEA1 Antibodies in Endosomal Transport and Signalling Studies
EEA1, or Early Endosome Antigen 1, is a phospholipid-binding protein essential for endosomal membrane trafficking and fusion, and is a useful endosomal marker. We at Novus Biologicals have an extensive EEA1 antibody catalog, with antibodies that have...  Read full blog post.

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Bioinformatics

Gene Symbol EEA1
Entrez
Uniprot