Rab5a Antibody

Images

 
Western Blot: Rab5a Antibody [NB120-13253] - analysis of Human Cell line lysates showing detection of Rab5 protein using Rabbit Anti-Rab5 Polyclonal Antibody. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. ...read more
Immunocytochemistry/ Immunofluorescence: Rab5a Antibody [NB120-13253] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody at 1:80 for ...read more
Immunohistochemistry-Paraffin: Rab5a Antibody [NB120-13253] - analysis using Rabbit Anti-Rab5 Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative Solution. Primary Antibody: Rabbit Anti-Rab5 ...read more
Immunocytochemistry/ Immunofluorescence: Rab5a Antibody [NB120-13253] - IF staining of Rab5a in hepatocytes. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Rab5a Antibody [NB120-13253] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody at 1:80 for ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Rab5a Antibody Summary

Immunogen
Human Rab5 synthetic peptide conjugated to KLH; identical to dog Rab5 sequence over the residues
Localization
Cell Membrane, Endosomone, Early Endosome Membrane, Melanosome
Marker
Early Endosome Marker
Specificity
Detects approx 26kDa.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAB5A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
Application Notes
1 ul/ml of Rab5 Antibody was sufficient for detection of Rab5 in 15 ug of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary Antibody. Use in Immunoprecipitation reported in scientific literature (PMID 23954637)
Positive Control
Brain Lysate (NB820-59705)
Brain Lysate (NB820-59657)
Rab5a Lysate (NBP2-65454)
Reviewed Applications
Read 2 Reviews rated 4
using
NB120-13253 in the following applications:

Publications
Read Publications using
NB120-13253 in the following applications:

  • IP
    1 publication
  • WB
    3 publications

Reactivity Notes

Based on homology, It's predicted to detect Rat, Bovine and Monkey. Mouse reactivity reported in scientific literature (PMID: 23954637).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Rab5a Antibody

  • RAB5A, member RAS oncogene family
  • RAB5RAS-associated protein RAB5A
  • ras-related protein Rab-5A

Background

Rab5 is a 24 kDa GTP-binding protein that regulates the fusion of plasma membrane -derived clathrin-coated vesicles with early endosomes, and homotypic fusion among early endosomes1. Localized to the cytoplasmic side of the plasma membrane, clathrin -coated vesicles and early endosomes, Rab5 appears to regulate vesicle fusion through a cycle of GDP/GTP e xchange and GTP hydrolysis. The different guanine nuc leotide binding states of Rab5 may affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. The GTP-bound, or active, form of Rab5 associates with membrane s and regulates vesicle docking and fusion. Studies using a Rab5 mutant that hydrolyzed xanthosine 5-triphosphate (XTP) indicated that nucleotide hydrolysis occurs even in the absence of membrane fusion. GTP hydrolysis by Rab5 may determine the frequency of membrane docking and fusion events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rab5a Antibody (NB120-13253)(8)

Reviews for Rab5a Antibody (NB120-13253) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB120-13253:
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WB
(1)
IF
(1)
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(1)
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot Rab5a NB120-13253
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reviewed by:
Anonymous
WB Human 12/15/2017
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Summary

ApplicationWestern Blot
Sample TestedHuman primary Colon Cancer cell line,human primary hepatocytes and Hep G2
SpeciesHuman
Lot140819

Comments

Commentshuman primary hepatocytes and Hep G2 cells
Name: Anti-Rab5a antibody (NB120-13253)
Catalog #: Anti-Rab5a antibody (NB120-13253)
Lot Number: Anti-Rab5a antibody (NB120-13253, Lot # 140819)
PO/Order Number: Click here to enter text..
WB Image Description (Please provide labels for all lanes): lane 1: human primary hepatocytes; lane 2: Hep G2
Sample Information:
     Cell Line or Tissue: human primary hepatocytes, Hep G2
     Species: human
     Treatment: No treatment
Lysate Preparation:
     Date of lysate preparation: December 11, 2017
     Lysis buffer used: 1X lysis buffer from Cell Signaling by adding PMSF
Reducing agent: beta-mercaptoethanol, DTT
If boiled (temperature/time): Yes
Controls:
     Positive Control: No
     Negative Control: No
     Loading Control (please attach additional images if applicable): No
Protein Amount Loaded per lane: 20 ug
Antibody Storage Conditions: -20℃
Electrophoresis:
Gel Percentage: 15%
Electrophoresis Conditions: Tris-Glycine-SDS at room temperature
     Voltage: 120V
     Time: 2 hours
Membrane Transfer:
     Method (Submersion/Semi-dry): wet transfer
     Membrane Type (PVDF/Nitrocellulose): Nitrocellulose
     Time: 2 hours
     Voltage: 100V
Blocking:
     Blocking Solution: 5% milk in 1X TBST
     Time: 1 hour at room temperature
Primary Antibody:
     Dilution: 1/1000
     Diluent Buffer: 2.5% BSA
     Incubation Time: overnight
     Incubation Temperature: 4℃
Washing Conditions:
     Wash Solution: 1X TBST
     Time and Repetitions: 5 min each for 3 times

Secondary Antibody
Manufacturer and Catalog #: Promega, W401B, Lot # 0000187662
Secondary description: goat anti-rabbit secondary antibody
     Dilution: 1/2000
     Diluent Buffer: 3% milk
     Incubation Time: 1 hour
     Incubation Temperature: room temperature
Detection Method:
Detection: ECL (GE, cat # RPN2209, lot # 9838243)
Procedure: Add equal volume of A and B, mix and apply on the membranes for 3-5 min before exposure
Development Time: 20 seconds
Molecular weight of band(s): ~ 23 kDa
Experimental Concerns and Observations: A specific band around 23 kDa was observed
Immunofluorescence Rab5a NB120-13253
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reviewed by:
Liyong Zhang
IF Mouse 08/19/2014
View

Summary

ApplicationImmunofluorescence
SpeciesMouse
Lot140110
FileView PDF

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rab5a Antibody (NB120-13253) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Anonymous
12/15/2017
Application: WB
Species: Human

Liyong Zhang
08/19/2014
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol RAB5A
Entrez
Uniprot