UVRAG Antibody

Images

 
Western Blot: UVRAG Antibody [NBP1-18885] - Beclin 1 is acetylated at lysines 430 and 437. TSA and NAM increase the binding of Beclin 1 to Rubicon. Immunoprecipitation of indicated Beclin 1-binding partners with ...read more
Immunohistochemistry-Paraffin: UVRAG Antibody [NBP1-18885] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti- UVRAG used at a dilution of 1:200 (1ug/ml). Detection: DAB
Independent Antibodies: Western Blot: UVRAG Antibody [NBP1-18885] - Whole cell lysate from HeLa and 293T cells. UVRAG was also immunoprecipitated by rabbit anti-UVRAG antibody NBP1-18884.
Western Blot: UVRAG Antibody [NBP1-18885] - Beclin 1 is acetylated at lysines 430 and 437. Mutations of K430 and K437 decrease the binding of Beclin 1 and Rubicon. Immunoprecipitation of indicated proteins with Beclin 1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

UVRAG Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 649 and 699 of human UV radiation resistance associated gene using the numbering given in entry NP_003360.2 (GeneID 7405).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UVRAG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 mcg/mg lysate
  • Knockout Validated
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in ICC/IF was reported in scientific literature (PMID: 30488027).
Publications
Read Publications using
NBP1-18885 in the following applications:

  • 1 publication
  • IP
    1 publication
  • KO
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UVRAG Antibody

  • beclin 1 binding protein
  • DHTX
  • disrupted in heterotaxy
  • p63
  • UV radiation resistance associated gene
  • UV radiation resistance-associated gene protein
  • UVRAG
  • VPS38

Background

This gene complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a protein with a C2 domain. The protein activates the Beclin1-PI(3)KC3 complex, promoting autophagy and suppressing the proliferation and tumorigenicity of human colon cancer cells. Chromosomal aberrations involving this gene are associated with left-right axis malformation and mutations in this gene have been associated with colon cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UVRAG Antibody (NBP1-18885)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC/IF, IP, KO, WB.


Filter By Application
ICC/IF
(1)
IP
(1)
KO
(1)
WB
(1)
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Filter By Species
Human
(2)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for UVRAG Antibody (NBP1-18885) (0)

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Secondary Antibodies

 

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Blogs on UVRAG.

UVRAG - A regulator of membrane trafficking in autophagy and endocytosis
UV resistance-associated gene (UVRAG) is a tumor suppressor that is commonly mutated in colon and breast cancer. While UVRAG was discovered for its ability to complement UV sensitivity in xeroderma pigmentosum cells, its main functions are in auto...  Read full blog post.

TFEB - An essential regulator of lysosome biogenesis
Transcription factor EB (TFEB) is a member of the MiTF/TFE (Microphthalmia/TFE) subfamily of basic/helix-loop-helix/leucine zipper transcription factors. This group of proteins is involved in the proliferation and development of specific cell type...  Read full blog post.

Beclin 2, a mammal-specific homolog of Beclin 1 with unique functional similarities and differences
Beclin 2 (BECN2) is also called Beclin-1-like protein 1/ BECN1P1 and it was recently identified by He et al 2013 as a mammal-specific homolog of the evolutionarily conserved protein Beclin 1 which is well established for its role in the regulation...  Read full blog post.

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Bioinformatics

Gene Symbol UVRAG
Entrez
Uniprot