PIK3CA Antibody

Western Blot: PIK3CA Antibody [NBP2-19804] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry/Immunofluorescence: PIK3CA Antibody [NBP2-19804] - HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PI3 kinase p110 alpha protein stained by PI3 kinase p110 alpha antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: PIK3CA Antibody [NBP2-19804] - Human Breast Cancer, using PI3 kinase p110 alpha antibody. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: PIK3CA Antibody [NBP2-19804] - PIK3CA Antibody Sample (50 ug of whole cell lysate) A: C2C12 B: C2C12+AEA10mM NBP2-19804 diluted at 1:1000.

Western Blot: PIK3CA Antibody [NBP2-19804] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 alpha antibody diluted at 1:3000.

• Reactivity: Hu, Mu, Rt, Bv, Ch, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PIK3CA Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human PI3 kinase p110 alpha. The exact sequence is proprietary.
• Predicted Species: Rat (99%), Chicken (95%), Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIK3CA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 124 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for PIK3CA Antibody

• CLAPO
• CLOVE
• CWS5
• EC 2.7.1
• EC 2.7.1.153
• MCMTC
• MGC142161
• MGC142163
• P110alpha
• p110-alpha
• Phosphatidylinositol 3-Kinase catalytic subunit alpha
• phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha
• phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
• Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha
• phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
• phosphoinositide-3-kinase, catalytic, alpha polypeptide
• PI3K
• PI3Kalpha
• PI3K-alpha
• PI3-kinase p110 subunit alpha
• PI3-kinase subunit alpha
• PIK3CA
• PtdIns-3-kinase p110
• PtdIns-3-kinase subunit alpha
• PtdIns-3-kinase subunit p110-alpha

Background

PIK3CA Antibody is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PIK3CA Antibody (NBP2-19804)(3)

Publications using NBP2-19804

• Chen YH, Wu JX, Yang SF et al. Metformin Potentiates the Anticancer Effect of Everolimus on Cervical Cancer In Vitro and In Vivo Cancers 2021-09-14 [PMID: 34572837] (WB, Mouse)
• Chen YH, Huang YC, Yang SF et al. Pitavastatin and metformin synergistically activate apoptosis and autophagy in pancreatic cancer cells Environmental toxicology 2021-04-22 [PMID: 33886150]
• Chen YH, Yang SF, Yang CK et al. Metformin induces apoptosis and inhibits migration by activating the AMPK/p53 axis and suppressing PI3K/AKT signaling in human cervical cancer cells Mol Med Rep 2021-01-01 [PMID: 33236135] (WB, Human)
  
  Details:
  Western blot analysis performed on CaSki, HeLa and C33A human cell lines.

Bioinformatics

• Gene Symbol: PIK3CA
• Uniprot:
  • P42336(Human)